Q2FGF8 (SYG_STAA3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycine--tRNA ligase
EC=6.1.1.14

Alternative name(s):
Glycyl-tRNA synthetase
Short name=GlyRS

Gene names

Name:	glyQS
Ordered Locus Names:	SAUSA300_1525

Organism

Staphylococcus aureus (strain USA300) [Complete proteome] [HAMAP]

Taxonomic identifier

367830 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Staphylococcaceae › Staphylococcus › Staphylococcus aureus › Staphylococcus aureus subsp. aureus

Protein attributes

Sequence length	463 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of glycine to tRNA(Gly) By similarity. HAMAP-Rule MF_00253
Catalytic activity	ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-tRNA(Gly). HAMAP-Rule MF_00253
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	glycyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP glycine-tRNA ligase activity Inferred from sequence or structural similarity. Source: UniProtKB protein dimerization activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 463	463	Glycine--tRNA ligase HAMAP-Rule MF_00253		PRO_1000047384
Regions
	Nucleotide binding	206 – 208	3	ATP By similarity
	Nucleotide binding	216 – 221	6	ATP By similarity
	Nucleotide binding	290 – 291	2	ATP By similarity
	Nucleotide binding	334 – 337	4	ATP By similarity
	Region	221 – 225	5	Substrate binding By similarity
	Region	330 – 334	5	Substrate binding By similarity
Sites
	Binding site	98	1	Substrate By similarity
	Binding site	174	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2FGF8 [UniParc]. Last modified March 21, 2006. Version 1. Checksum: F873C180BC51C467	FASTA	463	53,620
	10 20 30 40 50 60 MAKDMDTIVS LAKHRGFVFP GSDIYGGLSN TWDYGPLGVE LKNNVKKAWW QKFITQSPFN 70 80 90 100 110 120 VGIDAAILMN PKVWEASGHL NNFNDPMIDN KDSKIRYRAD KLIEDYMQDV KGDENFIADG 130 140 150 160 170 180 LSFEQMKKII DDEGIVCPVS KTANWTEIRQ FNLMFKTFQG VTEDSTNEIF LRPETAQGIF 190 200 210 220 230 240 VNYKNVQRSM RKKLPFGIGQ IGKSFRNEIT PGNFIFRTRE FEQMELEFFC KPGEEIEWQN 250 260 270 280 290 300 YWKTFASDWL TSLNMSSENM RLRDHDEDEL SHYSNATTDI EYKFPFGWGE LWGIASRTDF 310 320 330 340 350 360 DLRKHAEHSG EDFRYHDPET NEKYIPYCIE PSLGADRVTL AFLCDAYDEE GVEGSKDART 370 380 390 400 410 420 VLHFHPALAP YKAAILPLSK KLSGEAIKIF EQLSSKFSID FDESQSIGKR YRRQDEIGTP 430 440 450 460 YCVTFDFDSL EDNQVTVRDR DSMEQVRMPI SELEAFLTEK TKF

References

[1]

"Complete genome sequence of USA300, an epidemic clone of community-acquired meticillin-resistant Staphylococcus aureus." Diep B.A., Gill S.R., Chang R.F., Phan T.H., Chen J.H., Davidson M.G., Lin F., Lin J., Carleton H.A., Mongodin E.F., Sensabaugh G.F., Perdreau-Remington F. Lancet 367:731-739(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: USA300.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000255 Genomic DNA. Translation: ABD22244.1.
RefSeq	YP_494220.1. NC_007793.1.
3D structure databases
HSSP	HSSP built from PDB template 2G4C based on UniProtKB Q9UHN1.
ProteinModelPortal	Q2FGF8.
SMR	Q2FGF8. Positions 2-463.
ModBase	Search...
Protein-protein interaction databases
STRING	451515.SAUSA300_1525.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABD22244; ABD22244; SAUSA300_1525.
GeneID	3913901.
KEGG	saa:SAUSA300_1525.
PATRIC	19592475. VBIStaAur129981_1666.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0423.
HOGENOM	HOG000242016.
KO	K01880.
OMA	DLSYFDQ.
ProtClustDB	PRK04173.
Enzyme and pathway databases
BioCyc	SAUR451515:GH3C-1578-MONOMER.
Family and domain databases
Gene3D	3.40.50.800. 1 hit.
HAMAP	MF_00253_B. Gly_tRNA_synth_B.
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-dom. IPR006195. aa-tRNA-synth_II. IPR004154. Anticodon-bd. IPR027031. Gly-tRNA_synthase/POLG2. IPR022961. Gly_tRNA_ligase_bac. IPR002315. tRNA-synt_gly. [Graphical view]
PANTHER	PTHR10745. PTHR10745. 1 hit.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF00587. tRNA-synt_2b. 1 hit. [Graphical view]
PRINTS	PR01043. TRNASYNTHGLY.
SUPFAM	SSF52954. Anticodon_bd. 1 hit.
TIGRFAMs	TIGR00389. glyS_dimeric. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYG_STAA3

Accession

Primary (citable) accession number: Q2FGF8

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	March 21, 2006
Last modified:	May 1, 2013
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program