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Protein

Queuine tRNA-ribosyltransferase

Gene

tgt

Organism
Staphylococcus aureus (strain USA300)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Exchanges the guanine residue with 7-aminomethyl-7-deazaguanine in tRNAs with GUN anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine).UniRule annotation

Catalytic activityi

Guanine(34) in tRNA + queuine = queuosine(34) in tRNA + guanine.UniRule annotation
Guanine(34) in tRNA + 7-aminomethyl-7-carbaguanine = 7-aminomethyl-7-carbaguanine(34) in tRNA + guanine.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Pathwayi: tRNA-queuosine biosynthesis

This protein is involved in the pathway tRNA-queuosine biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway tRNA-queuosine biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei94 – 941NucleophileUniRule annotation
Binding sitei95 – 951SubstrateUniRule annotation
Metal bindingi306 – 3061ZincUniRule annotation
Metal bindingi308 – 3081ZincUniRule annotation
Metal bindingi311 – 3111ZincUniRule annotation
Metal bindingi337 – 3371ZincUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Queuosine biosynthesis, tRNA processing

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciSAUR451515:GH3C-1595-MONOMER.
UniPathwayiUPA00392.

Names & Taxonomyi

Protein namesi
Recommended name:
Queuine tRNA-ribosyltransferaseUniRule annotation (EC:2.4.2.29UniRule annotation)
Alternative name(s):
Guanine insertion enzymeUniRule annotation
tRNA-guanine transglycosylaseUniRule annotation
Gene namesi
Name:tgtUniRule annotation
Ordered Locus Names:SAUSA300_1595
OrganismiStaphylococcus aureus (strain USA300)
Taxonomic identifieri367830 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000001939 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 379379Queuine tRNA-ribosyltransferasePRO_1000016862Add
BLAST

Proteomic databases

PRIDEiQ2FG88.

Structurei

3D structure databases

ProteinModelPortaliQ2FG88.
SMRiQ2FG88. Positions 13-369.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the queuine tRNA-ribosyltransferase family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000223473.
KOiK00773.
OMAiMGVGKPD.
OrthoDBiEOG6SNDVG.

Family and domain databases

Gene3Di3.20.20.105. 1 hit.
HAMAPiMF_00168. Q_tRNA_Tgt.
InterProiIPR004803. Queuine_tRNA-ribosylTrfase.
IPR002616. tRNA_ribo_trans-like.
[Graphical view]
PfamiPF01702. TGT. 1 hit.
[Graphical view]
SUPFAMiSSF51713. SSF51713. 1 hit.
TIGRFAMsiTIGR00430. Q_tRNA_tgt. 1 hit.
TIGR00449. tgt_general. 1 hit.

Sequencei

Sequence statusi: Complete.

Q2FG88-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPAVTYEHIK TCKQSGARLG IVHTPHGSFE TPMFMPVGTK ATVKTMSPEE
60 70 80 90 100
LRQIEAKIIL GNTYHLWLQP GNDIIKHAGG LHKFMNWDGP ILTDSGGFQV
110 120 130 140 150
FSLSNLRKIT EEGVEFRHHT NGSKLFLSPE KSMQIQNDLG SDIMMAFDEC
160 170 180 190 200
PPMPAEYDYV KKSIERTTRW AKRCLDAHQR PEDQALFGII QGGEYEDLRE
210 220 230 240 250
QSAKDLVELD FPGYAIGGLS VGEPKPVMYK MVEHTEQFMP KDKPRYLMGV
260 270 280 290 300
GSPDALIECS IRGMDMFDCV LPTRIARNGT CMTSQGRLVI KNAKFADDLR
310 320 330 340 350
PLDENCDCYT CQNYSRAYIR HLIKAEETFG IRLTTIHNLH FLLKLMEDIR
360 370
QAIREDRLLD FKEEFFEQYG LNVENPKNF
Length:379
Mass (Da):43,310
Last modified:March 21, 2006 - v1
Checksum:i22E92081A3C147CD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000255 Genomic DNA. Translation: ABD22610.1.

Genome annotation databases

EnsemblBacteriaiABD22610; ABD22610; SAUSA300_1595.
KEGGisaa:SAUSA300_1595.
PATRICi19592623. VBIStaAur129981_1741.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000255 Genomic DNA. Translation: ABD22610.1.

3D structure databases

ProteinModelPortaliQ2FG88.
SMRiQ2FG88. Positions 13-369.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiQ2FG88.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABD22610; ABD22610; SAUSA300_1595.
KEGGisaa:SAUSA300_1595.
PATRICi19592623. VBIStaAur129981_1741.

Phylogenomic databases

HOGENOMiHOG000223473.
KOiK00773.
OMAiMGVGKPD.
OrthoDBiEOG6SNDVG.

Enzyme and pathway databases

UniPathwayiUPA00392.
BioCyciSAUR451515:GH3C-1595-MONOMER.

Family and domain databases

Gene3Di3.20.20.105. 1 hit.
HAMAPiMF_00168. Q_tRNA_Tgt.
InterProiIPR004803. Queuine_tRNA-ribosylTrfase.
IPR002616. tRNA_ribo_trans-like.
[Graphical view]
PfamiPF01702. TGT. 1 hit.
[Graphical view]
SUPFAMiSSF51713. SSF51713. 1 hit.
TIGRFAMsiTIGR00430. Q_tRNA_tgt. 1 hit.
TIGR00449. tgt_general. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Complete genome sequence of USA300, an epidemic clone of community-acquired meticillin-resistant Staphylococcus aureus."
    Diep B.A., Gill S.R., Chang R.F., Phan T.H., Chen J.H., Davidson M.G., Lin F., Lin J., Carleton H.A., Mongodin E.F., Sensabaugh G.F., Perdreau-Remington F.
    Lancet 367:731-739(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: USA300.

Entry informationi

Entry nameiTGT_STAA3
AccessioniPrimary (citable) accession number: Q2FG88
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: March 21, 2006
Last modified: March 16, 2016
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.