Reviewed, UniProtKB/Swiss-Prot Q2FFU9 (MENE_STAA3)

Names and origin

Protein names

Recommended name:
    2-succinylbenzoate--CoA ligase
    EC=6.2.1.26
Alternative name(s):
    OSB-CoA synthetase
    o-succinylbenzoyl-CoA synthetase

Gene names

Name:	menE
Ordered Locus Names:	SAUSA300_1737

Organism

Staphylococcus aureus (strain USA300) [Complete proteome] [HAMAP]

Taxonomic identifier

367830 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	492 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Converts 2-succinylbenzoate (OSB) to 2-succinylbenzoyl-CoA (OSB-CoA). HAMAP MF_00731
Catalytic activity	ATP + 2-succinylbenzoate + CoA = AMP + diphosphate + 2-succinylbenzoyl-CoA. HAMAP MF_00731
Pathway	Cofactor biosynthesis; menaquinone biosynthesis; menaquinone from chorismate: step 5/8. HAMAP MF_00731
Sequence similarities	Belongs to the ATP-dependent AMP-binding enzyme family. MenE type 1 subfamily.

Ontologies

Keywords
Biological process	Menaquinone biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	menaquinone biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW o-succinylbenzoate-CoA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 492	492	2-succinylbenzoate--CoA ligase HAMAP MF_00731		PRO_1000045966

Sequences

	Sequence		Length	Mass (Da)
	Q2FFU9-1 [UniParc]. Last modified March 21, 2006. Version 1. Checksum: 36D409105A4F7930	FASTA	492	55,415
	10 20 30 40 50 60 MDFWLYKQAQ QNGHHIAITD GQESYTYQNL YCEASLLAKR LKAYQQSRVG LYIDNSIQSI 70 80 90 100 110 120 ILIHACWLAN IEIAMINTRL TPNEMTNQMK SIDVQLIFCT LPLELRGFQI VSLDDIEFAG 130 140 150 160 170 180 RDITTNSLLD NTMGIQYETS NETVVPKESP SNILNTSFNL DDIASIMFTS GTTGPQKAVP 190 200 210 220 230 240 QTFRNHYASA IGCKESLGFD RDTNWLSVLP IYHISGLSVL LRAVIEGFTV RIVDKFNAEQ 250 260 270 280 290 300 ILTMIKNERI THISLVPQTL NWLMQQGLHE PYNLQKILLG GAKLSATMIE TALQYNLPIY 310 320 330 340 350 360 NSFGMTETCS QFLTATPEML HARPDTVGMP SANVDVKIKN PNKEGHGELM IKGANVMNVY 370 380 390 400 410 420 LYPTDLTGTF ENGYFNTGDI AEIDHEGYVM IYDRRKDLII SGGENIYPYQ IETVAKQFPG 430 440 450 460 470 480 ISDAVCVGHP DDTWGQVPKL YFVSESDISK AQLIAYLSQH LAKYKVPKHF EKVDTLPYTS 490 TGKLQRNKLY RG

References

[1]

"Complete genome sequence of USA300, an epidemic clone of community-acquired meticillin-resistant Staphylococcus aureus." Diep B.A., Gill S.R., Chang R.F., Phan T.H., Chen J.H., Davidson M.G., Lin F., Lin J., Carleton H.A., Mongodin E.F., Sensabaugh G.F., Perdreau-Remington F. Lancet 367:731-739(2006) [PubMed: 16517273] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000255 Genomic DNA. Translation: ABD22067.1.
RefSeq	YP_494429.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3913648.
GenomeReviews	Gene locus SAUSA300_1737 in contig CP000255_GR.
KEGG	saa:SAUSA300_1737.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q2FFU9.
OMA	Q2FFU9. DSPSNIL.
Family and domain databases
HAMAP	MF_00731. [Tree]
InterPro	IPR000873. AMP-dep_Synth/Lig. IPR010192. MenE. [Graphical view]
Pfam	PF00501. AMP-binding. 1 hit. [Graphical view]
TIGRFAMs	TIGR01923. menE. 1 hit.
PROSITE	PS00455. AMP_BINDING. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MENE_STAA3

Accession

Primary (citable) accession number: Q2FFU9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	March 21, 2006
Last modified:	June 16, 2009
This is version 18 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)