Reviewed, UniProtKB/Swiss-Prot Q2FFI7 (PUR8_STAA3)

Names and origin

Protein names

Recommended name:
    Adenylosuccinate lyase
      Short name=ASL
    EC=4.3.2.2
Alternative name(s):
    Adenylosuccinase
      Short name=ASase

Gene names

Name:	purB
Ordered Locus Names:	SAUSA300_1889

Organism

Staphylococcus aureus (strain USA300) [Complete proteome] [HAMAP]

Taxonomic identifier

367830 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	431 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	N(6)-(1,2-dicarboxyethyl)AMP = fumarate + AMP. (S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido)succinate = fumarate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide.
Pathway	Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 2/2. Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 5/5.
Sequence similarities	Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily.

Ontologies

Keywords
Biological process	Purine biosynthesis
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	purine ribonucleotide biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	(S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido)succinate AMP-lyase (fumarate-forming) activity Inferred from electronic annotation. Source: EC N6-(1,2-dicarboxyethyl)AMP AMP-lyase (fumarate-forming) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 431	431	Adenylosuccinate lyase		PRO_0000259981
Sites
	Active site	68	1	Proton donor By similarity
	Active site	141	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2FFI7-1 [UniParc]. Last modified March 21, 2006. Version 1. Checksum: 493F79CBE814B9E5	FASTA	431	49,603
	10 20 30 40 50 60 MIERYSREEM SNIWTDQNRY EAWLEVEILA CEAWSELGHI PKADVQKIRQ NAKVNVERAQ 70 80 90 100 110 120 EIEQETRHDV VAFTRQVSET LGEERKWVHY GLTSTDVVDT ALSFVIKQAN DIIEKDLERF 130 140 150 160 170 180 IDVLAEKAKN YKYTLMMGRT HGVHAEPTTF GVKMALWYTE MQRNLQRFKQ VREEIEVGKM 190 200 210 220 230 240 SGAVGTFANI PPEIESYVCK HLGIGTAPVS TQTLQRDRHA YYIATLALIA TSLEKFAVEI 250 260 270 280 290 300 RNLQKTETRE VEEAFAKGQK GSSAMPHKRN PIGSENITGI SRVIRGYITT AYENVPLWHE 310 320 330 340 350 360 RDISHSSAER IMLPDVTIAL DYALNRFTNI VDRLTVFEDN MRNNIDKTFG LIFSQRVLLA 370 380 390 400 410 420 LINKGMVREE AYDKVQPKAM ISWETKTPFR ELIEQDESIT SVLTKEELDE CFDPKHHLNQ 430 VDTIFERAGL A

References

[1]

"Complete genome sequence of USA300, an epidemic clone of community-acquired meticillin-resistant Staphylococcus aureus." Diep B.A., Gill S.R., Chang R.F., Phan T.H., Chen J.H., Davidson M.G., Lin F., Lin J., Carleton H.A., Mongodin E.F., Sensabaugh G.F., Perdreau-Remington F. Lancet 367:731-739(2006) [PubMed: 16517273] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000255 Genomic DNA. Translation: ABD20399.1.
RefSeq	YP_494541.1.
3D structure databases
SMR	Q2FFI7. Positions 2-430.
ModBase	Search...
Genome annotation databases
GeneID	3914957.
GenomeReviews	Gene locus SAUSA300_1889 in contig CP000255_GR.
KEGG	saa:SAUSA300_1889.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q2FFI7.
OMA	Q2FFI7. HPIDFRY.
Family and domain databases
InterPro	IPR019468. Adenylosuccinate_lyase_C. IPR003031. D_crystallin. IPR000362. Fumarate_lyase. IPR004769. Pur_lyase. [Graphical view]
Pfam	PF10397. ADSL_C. 1 hit. PF00206. Lyase_1. 1 hit. [Graphical view]
PRINTS	PR00145. DCRYSTALLIN. PR00149. FUMRATELYASE.
TIGRFAMs	TIGR00928. purB. 1 hit.
PROSITE	PS00163. FUMARATE_LYASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR8_STAA3

Accession

Primary (citable) accession number: Q2FFI7

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 14, 2006
Last sequence update:	March 21, 2006
Last modified:	June 16, 2009
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)