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Q2FEN5

- TOP3_STAA3

UniProt

Q2FEN5 - TOP3_STAA3

Protein

DNA topoisomerase 3

Gene

topB

Organism
Staphylococcus aureus (strain USA300)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 61 (01 Oct 2014)
      Sequence version 1 (21 Mar 2006)
      Previous versions | rss
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    Functioni

    Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.UniRule annotation

    Catalytic activityi

    ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi8 – 81Magnesium; catalyticUniRule annotation
    Sitei60 – 601Interaction with DNAUniRule annotation
    Metal bindingi104 – 1041Magnesium; catalyticUniRule annotation
    Sitei167 – 1671Interaction with DNAUniRule annotation
    Sitei175 – 1751Interaction with DNAUniRule annotation
    Active sitei305 – 3051O-(5'-phospho-DNA)-tyrosine intermediateUniRule annotation
    Sitei307 – 3071Interaction with DNAUniRule annotation

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. DNA topoisomerase type I activity Source: UniProtKB-HAMAP
    3. magnesium ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. DNA topological change Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Isomerase, Topoisomerase

    Keywords - Ligandi

    DNA-binding, Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciSAUR451515:GH3C-2208-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA topoisomerase 3UniRule annotation (EC:5.99.1.2UniRule annotation)
    Alternative name(s):
    DNA topoisomerase IIIUniRule annotation
    Gene namesi
    Name:topBUniRule annotation
    Ordered Locus Names:SAUSA300_2208
    OrganismiStaphylococcus aureus (strain USA300)
    Taxonomic identifieri367830 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus
    ProteomesiUP000001939: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. chromosome Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 711711DNA topoisomerase 3PRO_0000286367Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi451515.SAUSA300_2208.

    Structurei

    3D structure databases

    ProteinModelPortaliQ2FEN5.
    SMRiQ2FEN5. Positions 1-640.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini2 – 135134ToprimUniRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni186 – 1916Interaction with DNAUniRule annotation

    Sequence similaritiesi

    Belongs to the type IA topoisomerase family.UniRule annotation
    Contains 1 Toprim domain.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0551.
    HOGENOMiHOG000086847.
    KOiK03169.
    OMAiMTKHEER.
    OrthoDBiEOG60CWM6.

    Family and domain databases

    Gene3Di1.10.290.10. 1 hit.
    1.10.460.10. 2 hits.
    3.40.50.140. 1 hit.
    HAMAPiMF_00953. Topoisom_3_prok.
    InterProiIPR000380. Topo_IA.
    IPR003601. Topo_IA_2.
    IPR023406. Topo_IA_AS.
    IPR013497. Topo_IA_cen.
    IPR013824. Topo_IA_cen_sub1.
    IPR013826. Topo_IA_cen_sub3.
    IPR023405. Topo_IA_core_domain.
    IPR003602. Topo_IA_DNA-bd.
    IPR005738. TopoIII.
    IPR006171. Toprim_domain.
    [Graphical view]
    PANTHERiPTHR11390. PTHR11390. 1 hit.
    PfamiPF01131. Topoisom_bac. 1 hit.
    PF01751. Toprim. 1 hit.
    [Graphical view]
    PRINTSiPR00417. PRTPISMRASEI.
    SMARTiSM00437. TOP1Ac. 1 hit.
    SM00436. TOP1Bc. 1 hit.
    SM00493. TOPRIM. 1 hit.
    [Graphical view]
    SUPFAMiSSF56712. SSF56712. 1 hit.
    TIGRFAMsiTIGR01056. topB. 1 hit.
    PROSITEiPS00396. TOPOISOMERASE_I_PROK. 1 hit.
    PS50880. TOPRIM. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q2FEN5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKSLILAEKP SVARDIADAL QINQKRNGYF ENNQYIVTWA LGHLVTNATP    50
    EQYDKNLKEW RLEDLPIIPK YMKTVVIGKT SKQFKTVKAL ILDNKVKDII 100
    IATDAGREGE LVARLILDKV GNKKPIRRLW ISSVTKKAIQ QGFKNLKDGR 150
    QYNDLYYAAL ARSEADWIVG INATRALTTK YDAQLSLGRV QTPTIQLVNT 200
    RQQEINQFKP QQYFTLSLTV KGFDFQLESN QRYTNKETLE QMVNNLKNVD 250
    GKIKSVATKH KKSYPQSLYN LTDLQQDMYR RYKIGPKETL NTLQSLYERH 300
    KVVTYPRTDS NYLTTDMVDT MKERIQATMA TTYKDQARPL MSKTFSSKMS 350
    IFNNQKVSDH HAIIPTEVRP VMSDLSNREL KLYDMIVERF LEALMPPHEY 400
    DAITVTLEVA GHTFVLKENV TTVLGFKSIR QGESITEMQQ PFSEGDEVKI 450
    SKTNIREHET TPPEYFNEGS LLKAMENPQN FIQLKDKKYA QTLKQTGGIG 500
    TVATRADIID KLFNMNAIES RDGKIKVTSK GKQILELAPE ELTSPLLTAQ 550
    WEEKLLLIER GKYQAKTFIN EMKDFTKDVV NGIKNSDRKY KHDNLTTTEC 600
    PTCGKFMIKV KTKNGQMLVC QDPSCKTKKN VQRKTNARCP NCKKKLTLFG 650
    KGKEAVYRCV CGHSETQAHM DQRMKSKSSG KVSRKEMKKY MNKNEGLDNN 700
    PFKDALKNLN L 711
    Length:711
    Mass (Da):81,524
    Last modified:March 21, 2006 - v1
    Checksum:i9C67B5BAC841CBB6
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000255 Genomic DNA. Translation: ABD21681.1.
    RefSeqiYP_494843.1. NC_007793.1.

    Genome annotation databases

    EnsemblBacteriaiABD21681; ABD21681; SAUSA300_2208.
    GeneIDi3914904.
    KEGGisaa:SAUSA300_2208.
    PATRICi19593946. VBIStaAur129981_2349.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000255 Genomic DNA. Translation: ABD21681.1 .
    RefSeqi YP_494843.1. NC_007793.1.

    3D structure databases

    ProteinModelPortali Q2FEN5.
    SMRi Q2FEN5. Positions 1-640.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 451515.SAUSA300_2208.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABD21681 ; ABD21681 ; SAUSA300_2208 .
    GeneIDi 3914904.
    KEGGi saa:SAUSA300_2208.
    PATRICi 19593946. VBIStaAur129981_2349.

    Phylogenomic databases

    eggNOGi COG0551.
    HOGENOMi HOG000086847.
    KOi K03169.
    OMAi MTKHEER.
    OrthoDBi EOG60CWM6.

    Enzyme and pathway databases

    BioCyci SAUR451515:GH3C-2208-MONOMER.

    Family and domain databases

    Gene3Di 1.10.290.10. 1 hit.
    1.10.460.10. 2 hits.
    3.40.50.140. 1 hit.
    HAMAPi MF_00953. Topoisom_3_prok.
    InterProi IPR000380. Topo_IA.
    IPR003601. Topo_IA_2.
    IPR023406. Topo_IA_AS.
    IPR013497. Topo_IA_cen.
    IPR013824. Topo_IA_cen_sub1.
    IPR013826. Topo_IA_cen_sub3.
    IPR023405. Topo_IA_core_domain.
    IPR003602. Topo_IA_DNA-bd.
    IPR005738. TopoIII.
    IPR006171. Toprim_domain.
    [Graphical view ]
    PANTHERi PTHR11390. PTHR11390. 1 hit.
    Pfami PF01131. Topoisom_bac. 1 hit.
    PF01751. Toprim. 1 hit.
    [Graphical view ]
    PRINTSi PR00417. PRTPISMRASEI.
    SMARTi SM00437. TOP1Ac. 1 hit.
    SM00436. TOP1Bc. 1 hit.
    SM00493. TOPRIM. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56712. SSF56712. 1 hit.
    TIGRFAMsi TIGR01056. topB. 1 hit.
    PROSITEi PS00396. TOPOISOMERASE_I_PROK. 1 hit.
    PS50880. TOPRIM. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete genome sequence of USA300, an epidemic clone of community-acquired meticillin-resistant Staphylococcus aureus."
      Diep B.A., Gill S.R., Chang R.F., Phan T.H., Chen J.H., Davidson M.G., Lin F., Lin J., Carleton H.A., Mongodin E.F., Sensabaugh G.F., Perdreau-Remington F.
      Lancet 367:731-739(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: USA300.

    Entry informationi

    Entry nameiTOP3_STAA3
    AccessioniPrimary (citable) accession number: Q2FEN5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 2007
    Last sequence update: March 21, 2006
    Last modified: October 1, 2014
    This is version 61 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3