Reviewed, UniProtKB/Swiss-Prot Q2FDU6 (CRTN_STAA3)

Names and origin

Protein names

Recommended name:
Dehydrosqualene desaturase
EC=1.14.99.-

Alternative name(s):
Diapophytoene desaturase
4,4'-diapophytoene desaturase

Gene names

Name:	crtN
Ordered Locus Names:	SAUSA300_2498

Organism

Staphylococcus aureus (strain USA300) [Complete proteome] [HAMAP]

Taxonomic identifier

367830 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	502 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes three successive dehydrogenation reactions that lead to the introduction of three double bonds into dehydrosqualene (4,4'-diapophytoene), with 4,4'-diapophytofluene and 4,4'-diapo-zeta-carotene as intermediates, and 4,4'-diaponeurosporene (the major deep-yellow pigment in staphylococci strains) as the end product. Those are initial steps in the biosynthesis of staphyloxanthin, an orange carotenoid present in most staphylococci strains By similarity.
Cofactor	FAD By similarity.
Pathway	Carotenoid biosynthesis; staphyloxanthin biosynthesis; staphyloxanthin from farnesyl diphosphate: step 2/5.
Sequence similarities	Belongs to the carotenoid/retinoid oxidoreductase family. CrtN subfamily.

Ontologies

Keywords
Biological process	Carotenoid biosynthesis
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	carotenoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW chlorophyll biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW photosynthesis Inferred from electronic annotation. Source: InterPro
Molecular function	oxidoreductase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 502	502	Dehydrosqualene desaturase		PRO_0000272190
Regions
	Nucleotide binding	5 – 17	13	FAD Potential

Sequences

	Sequence		Length	Mass (Da)
	Q2FDU6-1 [UniParc]. Last modified March 21, 2006. Version 1. Checksum: 96952B497B394203	FASTA	502	56,742
	10 20 30 40 50 60 MKIAVIGAGV TGLAAAARIA SQGHEVTIFE KNNNVGGRMN QLKKDGFTFD MGPTIVMMPD 70 80 90 100 110 120 VYKDVFTACG KNYEDYIELR QLRYIYDVYF DHDDRITVPT DLAELQQMLE SIEPGSTHGF 130 140 150 160 170 180 MSFLTDVYKK YEIARRYFLE RTYRKPSDFY NMTSLVQGAK LKTLNHADQL IEHYIDNEKI 190 200 210 220 230 240 QKLLAFQTLY IGIDPKRGPS LYSIIPMIEM MFGVHFIKGG MYGMAQGLAQ LNKDLGVNIE 250 260 270 280 290 300 LNAEIEQIII DPKFKRADAI KVNGDIRKFD KILCTADFPS VAESLMPDFA PIKKYPPHKI 310 320 330 340 350 360 ADLDYSCSAF LMYIGIDIDV TDQVRLHNVI FSDDFRGNIE EIFEGRLSYD PSIYVYVPAV 370 380 390 400 410 420 ADKSLAPEGK TGIYVLMPTP ELKTGSGIDW SDEALTQQIK EIIYRKLATI EVFEDIKSHI 430 440 450 460 470 480 VSETIFTPND FEQTYHAKFG SAFGLMPTLA QSNYYRPQNV SRDYKDLYFA GASTHPGAGV 490 500 PIVLTSAKIT VDEMIKDIER GV

References

[1]

"Complete genome sequence of USA300, an epidemic clone of community-acquired meticillin-resistant Staphylococcus aureus." Diep B.A., Gill S.R., Chang R.F., Phan T.H., Chen J.H., Davidson M.G., Lin F., Lin J., Carleton H.A., Mongodin E.F., Sensabaugh G.F., Perdreau-Remington F. Lancet 367:731-739(2006) [PubMed: 16517273] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000255 Genomic DNA. Translation: ABD20881.1.
RefSeq	YP_495132.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q2FDU6.
Genome annotation databases
GeneID	3912838.
GenomeReviews	Gene locus SAUSA300_2498 in contig CP000255_GR.
KEGG	saa:SAUSA300_2498.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1233.
HOGENOM	HBG735114.
OMA	LKAGIDW.
ProtClustDB	CLSK886071.
Family and domain databases
InterPro	IPR000759. Adrndx_reductase. IPR002937. Amino_oxidase. IPR014105. Zeta-phytoene_desaturase. [Graphical view]
Pfam	PF01593. Amino_oxidase. 1 hit. [Graphical view]
PRINTS	PR00419. ADXRDTASE.
TIGRFAMs	TIGR02734. crtI_fam. 1 hit.
PROSITE	PS00982. PHYTOENE_DH. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CRTN_STAA3

Accession

Primary (citable) accession number: Q2FDU6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 23, 2007
Last sequence update:	March 21, 2006
Last modified:	March 2, 2010
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)