Q2F8F7 (PAP1_ORFN2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Poly(A) polymerase catalytic subunit
EC=2.7.7.19

Alternative name(s):
Poly(A) polymerase large subunit
Short name=PAP-L

Gene names

Name:

PAPL

Organism

Orf virus (strain NZ2) (OV NZ-2)

Taxonomic identifier

10259 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Poxviridae › Chordopoxvirinae › Parapoxvirus

Virus host

Homo sapiens (Human) [TaxID: 9606]
Capra hircus (Goat) [TaxID: 9925]
Ovis aries (Sheep) [TaxID: 9940]

Protein attributes

Sequence length	472 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Polymerase that creates the 3'-poly(A) tail of mRNA's.
Catalytic activity	ATP + RNA(n) = diphosphate + RNA(n+1).
Subunit structure	Heterodimer of a large (catalytic) subunit and a small (regulatory) subunit By similarity.
Sequence similarities	Belongs to the poxviridae poly(A) polymerase catalytic subunit family.

Ontologies

Keywords
Biological process	Transcription mRNA processing
Ligand	ATP-binding Nucleotide-binding
Molecular function	Transferase
Gene Ontology (GO)
Biological process	mRNA processing Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW polynucleotide adenylyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 472	472	Poly(A) polymerase catalytic subunit		PRO_0000308937
Sites
	Active site	191	1	By similarity
	Active site	193	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2F8F7 [UniParc]. Last modified March 21, 2006. Version 1. Checksum: FBE20290D08336F9	FASTA	472	53,032
	10 20 30 40 50 60 MAPPVIEEYL GSRPSMERCV LLRAQRRNMT RVLNFDRKLF LSLVVKSRRR FFKKLGGSEK 70 80 90 100 110 120 EIAARIEEYF SRQRRLEKLG QILTVLELQS VIVSEFTRTL GSLTTPTPAI NAAVRRVDAP 130 140 150 160 170 180 RARALASRAL NSYAVLPPPE EAAPMARHKH SDLVEVVKRL VKEHLRRHNK RCVCYGSYAL 190 200 210 220 230 240 HLLNPEIEYG DIDMVQTNAR PFLINLAFLI YFVTGRQTVL LRVPYLKNYV VLQDEEGGHI 250 260 270 280 290 300 LDSFNVRQAT LRALPTLLVD NIYVLHPFVQ LMAMLKMFSQ TDRIRDLADN FDKARARMET 310 320 330 340 350 360 LLAFALEELG EAPADGRTPL PCAFLPPEGG ASRVLEADAR ALDCGFARAV VFLDEPALVQ 370 380 390 400 410 420 TLLDMGVQDD EIVDFESVSN SAFLVRDNTL FTYFSNTVLM DGDAVHDLSR RGVSAHIVMF 430 440 450 460 470 LLLTRHPAAE GAVRSMLGSL VSDARPVTSV VERERKTGTH GVIDIAKNVI TH

References

[1]

"Comparative analysis of genome sequences of three isolates of Orf virus reveals unexpected sequence variation." Mercer A.A., Ueda N., Friederichs S.M., Hofmann K., Fraser K.M., Bateman T., Fleming S.B. Virus Res. 116:146-158(2006) [PubMed: 16274827] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	DQ184476 Genomic DNA. Translation: ABA00535.1.
3D structure databases
ProteinModelPortal	Q2F8F7.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR024231. Pox_polyA_pol_nucTrfase. IPR004976. Poxvirus_polyA_pol_cat. IPR024397. Poxvirus_polyA_pol_cat_C. IPR024398. Poxvirus_polyA_pol_cat_N. [Graphical view]
Pfam	PF03296. Pox_polyA_pol. 1 hit. PF12629. Pox_polyA_pol_C. 1 hit. PF12630. Pox_polyA_pol_N. 1 hit. [Graphical view]
PIRSF	PIRSF015693. VAC-48L_nuct. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PAP1_ORFN2

Accession

Primary (citable) accession number: Q2F8F7

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 23, 2007
Last sequence update:	March 21, 2006
Last modified:	October 19, 2011
This is version 20 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program