Reviewed, UniProtKB/Swiss-Prot Q2EN76 (NDKB_PIG)

Names and origin

Protein names

Recommended name:
    Nucleoside diphosphate kinase B
      Short name=NDP kinase B
      Short name=NDK B
    EC=2.7.4.6

Gene names

Name:

NME2

Organism

Sus scrofa (Pig)

Taxonomic identifier

9823 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Suina › Suidae › Sus

Protein attributes

Sequence length	152 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. Negatively regulates Rho activity by interacting with AKAP13/LBC By similarity.
Catalytic activity	ATP + nucleoside diphosphate = ADP + nucleoside triphosphate.
Cofactor	Magnesium By similarity.
Subunit structure	Hexamer of two different chains: A and B (A6, A5B, A4B2, A3B3, A2B4, AB5, B6). Interacts with AKAP13 and CAPN8 By similarity.
Subcellular location	Cytoplasm. Cell membrane By similarity.
Sequence similarities	Belongs to the NDK family.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Cellular component	Cell membrane Cytoplasm Membrane
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Acetylation Phosphoprotein
Gene Ontology (GO)
Biological process	CTP biosynthetic process Inferred from electronic annotation. Source: InterPro GTP biosynthetic process Inferred from electronic annotation. Source: InterPro UTP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleoside diphosphate kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 152	152	Nucleoside diphosphate kinase B		PRO_0000250201
Regions
	Region	1 – 66	66	Interaction with AKAP13 By similarity
Sites
	Active site	118	1	Pros-phosphohistidine intermediate By similarity
	Binding site	12	1	ATP By similarity
	Binding site	60	1	ATP By similarity
	Binding site	88	1	ATP By similarity
	Binding site	94	1	ATP By similarity
	Binding site	105	1	ATP By similarity
	Binding site	115	1	ATP By similarity
Amino acid modifications
	Modified residue	12	1	N6-acetyllysine By similarity
	Modified residue	49	1	N6-acetyllysine By similarity
	Modified residue	52	1	Phosphotyrosine By similarity
	Modified residue	56	1	N6-acetyllysine By similarity
	Modified residue	85	1	N6-acetyllysine By similarity
	Modified residue	94	1	Phosphothreonine By similarity
	Modified residue	100	1	N6-acetyllysine By similarity
	Modified residue	124	1	N6-acetyllysine By similarity
	Modified residue	128	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2EN76-1 [UniParc]. Last modified March 21, 2006. Version 1. Checksum: 446D0DFAC8D47019	FASTA	152	17,176
	10 20 30 40 50 60 MAHAERTFIA VKPDGVQRGL VGEIIKRFEQ KGFRLVALKF LQASEELLKQ HYIDLKDRPF 70 80 90 100 110 120 FPGLVKYMGS GPVVAMVWEG LNVVKTGRVM LGETNPADSK PGTIRGDFCI QVGRNIIHGS 130 140 150 DSVKSAEKEI SLWFKPEELV EYKSCAFDWI YE

References

[1]	Chen C.H., Ding S.T. Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	DQ372081 mRNA. Translation: ABD18456.1.
RefSeq	NP_001038075.1.
UniGene	Ssc.2257
3D structure databases
SMR	Q2EN76. Positions 2-152.
ModBase	Search...
Genome annotation databases
GeneID	733683.
KEGG	ssc:733683.
Organism-specific databases
CTD	733683.
Phylogenomic databases
HOVERGEN	Q2EN76.
Enzyme and pathway databases
BRENDA	2.7.4.6. 249.
Family and domain databases
InterPro	IPR001564. Nuc_diP_kinase_core. [Graphical view]
Gene3D	G3DSA:3.30.70.141. NDK. 1 hit.
PANTHER	PTHR11349. Nuc_diP_kinase_core. 1 hit.
Pfam	PF00334. NDK. 1 hit. [Graphical view]
PRINTS	PR01243. NUCDPKINASE.
ProDom	PD001018. NDK. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00562. NDK. 1 hit. [Graphical view]
PROSITE	PS00469. NDP_KINASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NDKB_PIG

Accession

Primary (citable) accession number: Q2EN76

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 19, 2006
Last sequence update:	March 21, 2006
Last modified:	November 3, 2009
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)