Q2EMV9 (PAR14_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Poly [ADP-ribose] polymerase 14
Short name=PARP-14
EC=2.4.2.30

Alternative name(s):
Collaborator of STAT6
Short name=CoaSt6

Gene names

Name:	Parp14
Synonyms:	Kiaa1268

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	1817 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Has ADP-ribosyltransferase activity By similarity. Enhances STAT6-dependent transcription. Ref.1
Catalytic activity	NAD+ + (ADP-D-ribosyl)(n)-acceptor = nicotinamide + (ADP-D-ribosyl)(n+1)-acceptor.
Subunit structure	Interacts with STAT6. Ref.1
Subcellular location	Nucleus. Cytoplasm. Note: In steady state cells the protein is mostly nuclear. A minor proportion is detected in the cytoplasm. Ref.1
Tissue specificity	Weakly expressed in the thymus during development and in adulthood. Ref.6
Sequence similarities	Contains 3 Macro domains. Contains 1 PARP catalytic domain. Contains 1 WWE domain.
Sequence caution	The sequence AAH21340.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence AAH21340.1 differs from that shown. Reason: Contaminating sequence. Sequence of unknown origin in the N-terminal part.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Cytoplasm Nucleus
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Repeat
Ligand	NAD
Molecular function	Glycosyltransferase Transferase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NAD+ ADP-ribosyltransferase activity Inferred from electronic annotation. Source: EC nucleic acid binding Inferred from electronic annotation. Source: InterPro nucleotide binding Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Gpi	P06745	4	EBI-1534943,EBI-1534927

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q2EMV9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q2EMV9-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-589: Missing.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1817

1817

Poly [ADP-ribose] polymerase 14

PRO_0000247590

Regions

Domain

802 – 989

188

Macro 1

Domain

1014 – 1202

189

Macro 2

Domain

1227 – 1398

172

Macro 3

Domain

1539 – 1617

79

WWE

Domain

1621 – 1817

197

PARP catalytic

Compositional bias

771 – 800

30

Glu-rich

Natural variations

Alternative sequence

1 – 589

589

Missing in isoform 2.

VSP_020020

Natural variant

1384

1

P → S in strain: Czech II. Ref.5

Natural variant

1658

1

N → D in strain: Czech II. Ref.5

Natural variant

1682

1

S → G in strain: Czech II. Ref.5

Experimental info

Sequence conflict

708

1

N → Y in ABD16173. Ref.1

Sequence conflict

789

1

E → K in BAC40943. Ref.3

Sequence conflict

1045

1

S → T in ABD16173. Ref.1

Sequence conflict

1209

1

K → E in BAC40943. Ref.3

Sequence conflict

1609

1

F → L in ABD16173. Ref.1

Secondary structure

1

.

.

.

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.

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.

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1817

Helix Strand Turn

Details...

Beta strand

1532 – 1534

3

Turn

1537 – 1541

5

Helix

1542 – 1548

7

Beta strand

1552 – 1556

5

Beta strand

1561 – 1563

3

Helix

1568 – 1577

10

Beta strand

1581 – 1587

7

Beta strand

1590 – 1595

6

Turn

1596 – 1599

4

Beta strand

1600 – 1602

3

Beta strand

1608 – 1614

7

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified July 27, 2011. Version 3. Checksum: 42E902CC20858931	FASTA	1,817	203,802
	10 20 30 40 50 60 MAASGSFPLL VEGSWGPDPP KNLINKLQVY FQSRKKSGGG ECEVVPEPGN PARFRVLFSP 70 80 90 100 110 120 EDVRQNVLER GNHELVWQEK GTFKLTVLMP TDPEEASASK KSRKESPEEE SKTKEDAVKQ 130 140 150 160 170 180 GDLDITHSPS SGSEKTEDVP KECENISSMV AFENLPEKVS EMVLTILVEN ISGLPSDDFK 190 200 210 220 230 240 VEVNRDFAVA VVTFQKPIDI KKFIVDCISH RSNQQLQLAP RLLETTNVVR VENLPPGVDE 250 260 270 280 290 300 YQLQLFFENP FNGGGRVARV ECFPEESSAL VEFCDSKVLD TVMAKTHSYN KMPLSVFPYY 310 320 330 340 350 360 PSLGTALYGE EKPLIKLPAS FQESLDLPLW KFFQKNNHLI EEINNEMRCC HCELTWSEIN 370 380 390 400 410 420 GKLTIRPAAT LVNHRLSIKT WQRDASAVLS GIKSKYGVEL FEVCSPVWDI IKHELESGDD 430 440 450 460 470 480 RVLVEFEKES LNIAGKSEDV QGMSQKIREL IESTTEKLRR EEQSLKEKVA ISPGKHYLLH 490 500 510 520 530 540 HSGFLKDLSK GFPEMEISYD ATAQFLYLKG FRADVYKVKC DIQEKVFSMA QKDVQVSSEV 550 560 570 580 590 600 FEFLQQVDSQ RLSKSLFEAQ NILAIYELKG TALFLVGSSF KDLAEAETKM LSALSHKQIE 610 620 630 640 650 660 VEDKEVLISN GWKKKVHPLQ KRHSSCATII VQNELTSETP AKVIVTGCVK EVNEIHRQLF 670 680 690 700 710 720 EYLENNMKVE RALKIKPSLI VDYLRTDKRL LSKIKKAHVY VHFKPKDNPN SILLTGCKSK 730 740 750 760 770 780 VLECMNLVKE IQDSVCVQRF QTDKAGVRHF FKDKESYYKT EIGRQFGCVI ELEEDREEKG 790 800 810 820 830 840 EEEDGEEEEG EEEGESSINE QKCHLQRDIA PGVKLFVLEE DLSRFPVDVV VNAANENLKH 850 860 870 880 890 900 ISGLAQALSK AAGPELQTEC DQIVKEGGVV LPGNAVISKA GKLPCHHVIH AVGPRWKGDK 910 920 930 940 950 960 VLECVSLLKK VVRQSLSLAE EHRCRSIAMP AVSAGIFDFP LELCVANIVS AIKEHFQHKR 970 980 990 1000 1010 1020 DTHTLKKIYL VGLPAKVARA FAEAVKTTYK DSLSHTAFPS SLKALVPLGK TPQKQGSLLV 1030 1040 1050 1060 1070 1080 SPEGLRIRLV EEGVQNATTH AIVNSISPDL KLNKGPLSQA FLEKAGPKLQ EELTRSGQGV 1090 1100 1110 1120 1130 1140 SVDVGTILQT SGCNLNSRHV FHVVPPPWKS NNSAWSLKIM KNIIRDCLKT TENLSLQSIA 1150 1160 1170 1180 1190 1200 FPAIGTGNLR FPKPEFAKLI ISEVLKFSSR NQLKTLQEVQ FLLHPKDHEN IQAFSDEFDK 1210 1220 1230 1240 1250 1260 RNNGDPSDKN PKAEDTQGIY GSLSSPTLGM HEMNIGPILF QVATGNIIKE VADVIVNSTT 1270 1280 1290 1300 1310 1320 LTFDLKSGVS KAILEGAGQN VEQECSLLAK QSNHGYIVTG GGLLQCKNII HVVGGNDVKK 1330 1340 1350 1360 1370 1380 SVSCVLEECE QRNYSSICLP AIGTGNAQQD PNVVAKAIID AIEEFVQKKS VQAVKRVKVV 1390 1400 1410 1420 1430 1440 IFQPHILQFF YDNMKEREGS PAPPKQSPAK QSVMSKIASF LGFPKQASPK KNTLVLEKKI 1450 1460 1470 1480 1490 1500 EHTVFQVCGS GVDSVNKTIS WLKELITKEQ LSYTNDDECV SDFDMEEYEK LNEIQKELNI 1510 1520 1530 1540 1550 1560 TIEMNQKKTS IQVSGISRDV IKARDEIEGM IKSIRLAKEK ESQADYISTY VEWQYIDKNI 1570 1580 1590 1600 1610 1620 TQCFDKMTNM KLEVAWKAKK KDTVVQIHNQ DFTVDLSTNT ATAPQGQTFT VQRLVKAEAE 1630 1640 1650 1660 1670 1680 IPANWSDMKQ DKLLLVNLQT SDPEYNMVAS AFRQTCSNFF IEKIERIQNP ALWRRYQAYK 1690 1700 1710 1720 1730 1740 KSMDEKNGNV RNEKHLFHGT EASSLPQLNS NGFNRSYAGK NATAYGKGTY FAVKASYSAC 1750 1760 1770 1780 1790 1800 DTYSRPDTNG RKYMYYVRVL TGNYTNGNAS LIVPPSRDPQ NAADLYDTVT DNDKNPSIFV 1810 VFYDNQTYPE YLITFRQ
	Isoform 2 [UniParc]. Checksum: 3E066E6F69CD4193	FASTA	1,228	137,366
	10 20 30 40 50 60 MLSALSHKQI EVEDKEVLIS NGWKKKVHPL QKRHSSCATI IVQNELTSET PAKVIVTGCV 70 80 90 100 110 120 KEVNEIHRQL FEYLENNMKV ERALKIKPSL IVDYLRTDKR LLSKIKKAHV YVHFKPKDNP 130 140 150 160 170 180 NSILLTGCKS KVLECMNLVK EIQDSVCVQR FQTDKAGVRH FFKDKESYYK TEIGRQFGCV 190 200 210 220 230 240 IELEEDREEK GEEEDGEEEE GEEEGESSIN EQKCHLQRDI APGVKLFVLE EDLSRFPVDV 250 260 270 280 290 300 VVNAANENLK HISGLAQALS KAAGPELQTE CDQIVKEGGV VLPGNAVISK AGKLPCHHVI 310 320 330 340 350 360 HAVGPRWKGD KVLECVSLLK KVVRQSLSLA EEHRCRSIAM PAVSAGIFDF PLELCVANIV 370 380 390 400 410 420 SAIKEHFQHK RDTHTLKKIY LVGLPAKVAR AFAEAVKTTY KDSLSHTAFP SSLKALVPLG 430 440 450 460 470 480 KTPQKQGSLL VSPEGLRIRL VEEGVQNATT HAIVNSISPD LKLNKGPLSQ AFLEKAGPKL 490 500 510 520 530 540 QEELTRSGQG VSVDVGTILQ TSGCNLNSRH VFHVVPPPWK SNNSAWSLKI MKNIIRDCLK 550 560 570 580 590 600 TTENLSLQSI AFPAIGTGNL RFPKPEFAKL IISEVLKFSS RNQLKTLQEV QFLLHPKDHE 610 620 630 640 650 660 NIQAFSDEFD KRNNGDPSDK NPKAEDTQGI YGSLSSPTLG MHEMNIGPIL FQVATGNIIK 670 680 690 700 710 720 EVADVIVNST TLTFDLKSGV SKAILEGAGQ NVEQECSLLA KQSNHGYIVT GGGLLQCKNI 730 740 750 760 770 780 IHVVGGNDVK KSVSCVLEEC EQRNYSSICL PAIGTGNAQQ DPNVVAKAII DAIEEFVQKK 790 800 810 820 830 840 SVQAVKRVKV VIFQPHILQF FYDNMKEREG SPAPPKQSPA KQSVMSKIAS FLGFPKQASP 850 860 870 880 890 900 KKNTLVLEKK IEHTVFQVCG SGVDSVNKTI SWLKELITKE QLSYTNDDEC VSDFDMEEYE 910 920 930 940 950 960 KLNEIQKELN ITIEMNQKKT SIQVSGISRD VIKARDEIEG MIKSIRLAKE KESQADYIST 970 980 990 1000 1010 1020 YVEWQYIDKN ITQCFDKMTN MKLEVAWKAK KKDTVVQIHN QDFTVDLSTN TATAPQGQTF 1030 1040 1050 1060 1070 1080 TVQRLVKAEA EIPANWSDMK QDKLLLVNLQ TSDPEYNMVA SAFRQTCSNF FIEKIERIQN 1090 1100 1110 1120 1130 1140 PALWRRYQAY KKSMDEKNGN VRNEKHLFHG TEASSLPQLN SNGFNRSYAG KNATAYGKGT 1150 1160 1170 1180 1190 1200 YFAVKASYSA CDTYSRPDTN GRKYMYYVRV LTGNYTNGNA SLIVPPSRDP QNAADLYDTV 1210 1220 TDNDKNPSIF VVFYDNQTYP EYLITFRQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Selective potentiation of Stat-dependent gene expression by collaborator of Stat6 (CoaSt6), a transcriptional cofactor." Goenka S., Boothby M. Proc. Natl. Acad. Sci. U.S.A. 103:4210-4215(2006) [PubMed: 16537510] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, INTERACTION WITH STAT6, SUBCELLULAR LOCATION. Strain: BALB/c. Tissue: Spleen.
[2]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed: 19468303] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6.
[3]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-1393 (ISOFORM 2). Strain: NOD. Tissue: Spleen.
[4]	"Prediction of the coding sequences of mouse homologues of KIAA gene. The complete nucleotide sequences of mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries." Okazaki N., Kikuno R.F., Ohara R., Inamoto S., Seino S., Nishimura M., Nagase T., Ohara O., Koga H. Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 794-1817. Tissue: Pancreatic islet.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1382-1817, VARIANTS SER-1384; ASP-1658 AND GLY-1682. Strain: Czech II. Tissue: Mammary tumor.
[6]	"The macroPARP genes Parp-9 and Parp-14 are developmentally and differentially regulated in mouse tissues." Hakme A., Huber A., Dolle P., Schreiber V. Dev. Dyn. 237:209-215(2008) [PubMed: 18069692] [Abstract] Cited for: TISSUE SPECIFICITY.
[7]	"Solution structure of WWE domain in Parp14 protein." RIKEN structural genomics initiative (RSGI) Submitted (NOV-2005) to the PDB data bank Cited for: STRUCTURE BY NMR OF 1532-1617.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

DQ372930 mRNA. Translation: ABD16173.1.
AC129574 Genomic DNA. No translation available.
AK089685 mRNA. Translation: BAC40943.1.
AK220263 mRNA. Translation: BAD90188.1.
BC021340 mRNA. Translation: AAH21340.1. Different initiation.

IPI

IPI00551075.
IPI00776127.

RefSeq

NP_001034619.2. NM_001039530.3.

UniGene

Mm.244406.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1X4R	NMR	-	A	1532-1617	[»]

ProteinModelPortal

Q2EMV9.

SMR

Q2EMV9. Positions 803-989, 1008-1203, 1227-1398, 1534-1617, 1635-1817.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q2EMV9. 1 interaction.

STRING

Q2EMV9.

PTM databases

PhosphoSite

Q2EMV9.

Proteomic databases

PRIDE

Q2EMV9.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSMUST00000042665; ENSMUSP00000037657; ENSMUSG00000034422.

GeneID

547253.

KEGG

mmu:547253.

Organism-specific databases

CTD

54625.

MGI

MGI:1919489. Parp14.

Rouge

Search...

Phylogenomic databases

eggNOG

roNOG05330.

HOGENOM

HBG443548.

HOVERGEN

HBG082105.

InParanoid

Q2EMV9.

OrthoDB

EOG4HX505.

Gene expression databases

ArrayExpress

Q2EMV9.

Bgee

Q2EMV9.

CleanEx

MM_PARP14.

Genevestigator

Q2EMV9.

GermOnline

ENSMUSG00000034422. Mus musculus.

Family and domain databases

InterPro

IPR002589. A1pp.
IPR012677. Nucleotide-bd_a/b_plait.
IPR012317. Poly(ADP-ribose)pol_cat_dom.
IPR000504. RRM_dom.
IPR004170. WWE-dom.
[Graphical view]

Gene3D

G3DSA:3.30.70.330. a_b_plait_nuc_bd. 1 hit.

KO

K15261.

Pfam

PF01661. Macro. 3 hits.
PF00644. PARP. 1 hit.
[Graphical view]

SMART

SM00506. A1pp. 3 hits.
SM00360. RRM. 1 hit.
[Graphical view]

PROSITE

PS51154. MACRO. 3 hits.
PS51059. PARP_CATALYTIC. 1 hit.
PS50918. WWE. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

SOURCE

Search...

Entry information

Entry name

PAR14_MOUSE

Accession

Primary (citable) accession number: Q2EMV9
Secondary accession number(s): E9QP48 , Q571C6, Q8BN35, Q8VDT6

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2006
Last sequence update:	July 27, 2011
Last modified:	December 14, 2011
This is version 58 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program