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Protein

Response regulator inhibitor for tor operon

Gene

torI

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Transcription inhibitory protein for the torCAD operon. Also acts as an excisionase and plays an essential role in the defective prophage CPS53 excision.2 Publications

GO - Molecular functioni

  • DNA binding Source: EcoCyc
  • identical protein binding Source: EcoCyc
  • sequence-specific DNA binding Source: EcoCyc

GO - Biological processi

  • DNA excision Source: EcoCyc
  • DNA recombination Source: UniProtKB-KW
  • negative regulation of DNA-templated transcription, initiation Source: EcoCyc
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

DNA recombination, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciEcoCyc:MONOMER0-1641.
ECOL316407:JW5387-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Response regulator inhibitor for tor operon
Short name:
Tor inhibitor
Gene namesi
Name:torI
Ordered Locus Names:b4501, JW5387
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG14411. torI.

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi28 – 281Y → F or S: Loss of all excisionase activity. 1 Publication
Mutagenesisi45 – 451R → K or Q: Retains approximately 10% excisionase activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 6666Response regulator inhibitor for tor operonPRO_0000252144Add
BLAST

Proteomic databases

PaxDbiQ2EES9.

Interactioni

Subunit structurei

Interacts with TorR. Binds to the effector domain of TorR. This interaction, which does not interfere with TorR DNA binding activity, probably prevents the recruitment of RNA polymerase to the torCAD promoter. Binds to DNA.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
sixAP765023EBI-9154838,EBI-548621

GO - Molecular functioni

  • identical protein binding Source: EcoCyc

Protein-protein interaction databases

BioGridi4260552. 15 interactions.
IntActiQ2EES9. 1 interaction.
STRINGi511145.b4501.

Structurei

Secondary structure

1
66
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi7 – 115Combined sources
Helixi13 – 208Combined sources
Helixi24 – 3512Combined sources
Beta strandi39 – 446Combined sources
Beta strandi46 – 494Combined sources
Helixi50 – 6314Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1Z4HNMR-A1-66[»]
ProteinModelPortaliQ2EES9.
SMRiQ2EES9. Positions 1-66.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ2EES9.

Family & Domainsi

Sequence similaritiesi

Belongs to the phage AlpA excisionase family.Curated

Phylogenomic databases

eggNOGiENOG41065VV. Bacteria.
ENOG41122W9. LUCA.
HOGENOMiHOG000252905.
OMAiCAFKSKL.
OrthoDBiEOG6R87M0.

Sequencei

Sequence statusi: Complete.

Q2EES9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQHELQPDSL VDLKFIMADT GFGKTFIYDR IKSGDLPKAK VIHGRARWLY
60
RDHCEFKNKL LSRANG
Length:66
Mass (Da):7,678
Last modified:March 21, 2006 - v1
Checksum:i587B519EF433DC5B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: ABD18695.1.
AP009048 Genomic DNA. Translation: BAE76704.1.
RefSeqiWP_001163428.1. NZ_LN832404.1.
YP_588463.1. NC_000913.3.

Genome annotation databases

EnsemblBacteriaiABD18695; ABD18695; b4501.
BAE76704; BAE76704; BAE76704.
GeneIDi1450232.
KEGGiecj:JW5387.
eco:b4501.
PATRICi32120105. VBIEscCol129921_2461.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: ABD18695.1.
AP009048 Genomic DNA. Translation: BAE76704.1.
RefSeqiWP_001163428.1. NZ_LN832404.1.
YP_588463.1. NC_000913.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1Z4HNMR-A1-66[»]
ProteinModelPortaliQ2EES9.
SMRiQ2EES9. Positions 1-66.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260552. 15 interactions.
IntActiQ2EES9. 1 interaction.
STRINGi511145.b4501.

Proteomic databases

PaxDbiQ2EES9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABD18695; ABD18695; b4501.
BAE76704; BAE76704; BAE76704.
GeneIDi1450232.
KEGGiecj:JW5387.
eco:b4501.
PATRICi32120105. VBIEscCol129921_2461.

Organism-specific databases

EcoGeneiEG14411. torI.

Phylogenomic databases

eggNOGiENOG41065VV. Bacteria.
ENOG41122W9. LUCA.
HOGENOMiHOG000252905.
OMAiCAFKSKL.
OrthoDBiEOG6R87M0.

Enzyme and pathway databases

BioCyciEcoCyc:MONOMER0-1641.
ECOL316407:JW5387-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ2EES9.
PROiQ2EES9.

Family and domain databases

ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  2. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. "TorI, a response regulator inhibitor of phage origin in Escherichia coli."
    Ansaldi M., Theraulaz L., Mejean V.
    Proc. Natl. Acad. Sci. U.S.A. 101:9423-9428(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN TOR OPERON REGULATION, INTERACTION WITH TORR.
  4. "Structural and genetic analyses reveal a key role in prophage excision for the TorI response regulator inhibitor."
    Elantak L., Ansaldi M., Guerlesquin F., Mejean V., Morelli X.
    J. Biol. Chem. 280:36802-36808(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR, FUNCTION AS AN EXCISIONASE, MUTAGENESIS OF TYR-28 AND ARG-45.

Entry informationi

Entry nameiTORI_ECOLI
AccessioniPrimary (citable) accession number: Q2EES9
Secondary accession number(s): Q2MAK2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 3, 2006
Last sequence update: March 21, 2006
Last modified: March 16, 2016
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.