Q2EES9 (TORI_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Response regulator inhibitor for tor operon
Short name=Tor inhibitor

Gene names

Name:	torI
Ordered Locus Names:	b4501, JW5387

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	66 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Transcription inhibitory protein for the torCAD operon. Also acts as an excisionase and plays an essential role in the defective prophage CPS53 excision. Ref.3 Ref.4
Subunit structure	Interacts with TorR. Binds to the effector domain of TorR. This interaction, which does not interfere with TorR DNA binding activity, probably prevents the recruitment of RNA polymerase to the torCAD promoter. Binds to DNA. Ref.3
Sequence similarities	Belongs to the phage AlpA excisionase family.

Ontologies

Keywords
Biological process	DNA recombination Transcription Transcription regulation
Ligand	DNA-binding
Molecular function	Repressor
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA excision Inferred from mutant phenotype Ref.4. Source: EcoCyc DNA recombination Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of DNA-dependent transcription, initiation Inferred from mutant phenotype Ref.3. Source: EcoCyc transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 66

66

Response regulator inhibitor for tor operon

PRO_0000252144

Experimental info

Mutagenesis

28

1

Y → F or S: Loss of all excisionase activity. Ref.4

Mutagenesis

45

1

R → K or Q: Retains approximately 10% excisionase activity. Ref.4

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

66

Helix Strand Turn

Details...

Beta strand

7 – 11

5

Helix

13 – 20

8

Helix

24 – 35

12

Beta strand

39 – 44

6

Beta strand

46 – 49

4

Helix

50 – 63

14

Sequences

	Sequence		Length	Mass (Da)
	Q2EES9 [UniParc]. Last modified March 21, 2006. Version 1. Checksum: 587B519EF433DC5B	FASTA	66	7,678
	10 20 30 40 50 60 MQHELQPDSL VDLKFIMADT GFGKTFIYDR IKSGDLPKAK VIHGRARWLY RDHCEFKNKL LSRANG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[2]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"TorI, a response regulator inhibitor of phage origin in Escherichia coli." Ansaldi M., Theraulaz L., Mejean V. Proc. Natl. Acad. Sci. U.S.A. 101:9423-9428(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION IN TOR OPERON REGULATION, INTERACTION WITH TORR.
[4]	"Structural and genetic analyses reveal a key role in prophage excision for the TorI response regulator inhibitor." Elantak L., Ansaldi M., Guerlesquin F., Mejean V., Morelli X. J. Biol. Chem. 280:36802-36808(2005) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR, FUNCTION AS AN EXCISIONASE, MUTAGENESIS OF TYR-28 AND ARG-45.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U00096 Genomic DNA. Translation: ABD18695.1.
AP009048 Genomic DNA. Translation: BAE76704.1.

RefSeq

YP_490605.1. NC_007779.1.
YP_588463.1. NC_000913.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1Z4H	NMR	-	A	1-66	[»]

ProteinModelPortal

Q2EES9.

SMR

Q2EES9. Positions 1-66.

ModBase

Search...

Protein-protein interaction databases

STRING

511145.b4501.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

ABD18695; ABD18695; b4501.
BAE76704; BAE76704; BAE76704.

GeneID

12931558.
1450232.

KEGG

ecj:Y75_p2330.
eco:b4501.

PATRIC

32120105. VBIEscCol129921_2461.

Organism-specific databases

EcoGene

EG14411. torI.

Phylogenomic databases

eggNOG

NOG270238.

HOGENOM

HOG000252905.

OMA

RYRDHCE.

ProtClustDB

CLSK753952.

Enzyme and pathway databases

BioCyc

EcoCyc:MONOMER0-1641.
ECOL316407:JW5387-MONOMER.

Gene expression databases

Genevestigator

Q2EES9.

Family and domain databases

InterPro

IPR010260. Phage_P4_Orf88.
[Graphical view]

Pfam

PF05930. Phage_AlpA. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q2EES9.

Entry information

Entry name

TORI_ECOLI

Accession

Primary (citable) accession number: Q2EES9
Secondary accession number(s): Q2MAK2

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 3, 2006
Last sequence update:	March 21, 2006
Last modified:	May 1, 2013
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program