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Protein

Protein O-GlcNAcase

Gene

OG2516_04129

Organism
Oceanicola granulosus (strain ATCC BAA-861 / DSM 15982 / KCTC 12143 / HTCC2516)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cleaves GlcNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc and 4-methylumbelliferone-GlcNAc as substrate (in vitro).1 Publication

Catalytic activityi

[Protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-serine + H2O = [protein]-L-serine + N-acetyl-D-glucosamine.1 Publication
[Protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-threonine + H2O = [protein]-L-threonine + N-acetyl-D-glucosamine.1 Publication
Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides.1 Publication

Enzyme regulationi

Inhibited by PUGNac (O-(2-acetamido-2-deoxy-D-glucopyranosylidene)amino-N-phenylcarbamate).1 Publication

Kineticsi

  1. KM=8.5 mM for p-nitrophenyl-GlcNAc1 Publication
  2. KM=2.9 mM for 4-methylumbelliferone-GlcNAc1 Publication

    pH dependencei

    Optimum pH is 7.4-7.6.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei8 – 81Substrate; via carbonyl oxygenImported
    Binding sitei39 – 391SubstrateBy similarity
    Binding sitei115 – 1151SubstrateBy similarity
    Active sitei116 – 1161Proton donorBy similarity
    Binding sitei160 – 1601SubstrateImported
    Binding sitei226 – 2261SubstrateImported
    Binding sitei254 – 2541SubstrateImported

    GO - Molecular functioni

    • beta-N-acetylglucosaminidase activity Source: UniProtKB

    GO - Biological processi

    • carbohydrate metabolic process Source: UniProtKB
    • protein deglycosylation Source: UniProtKB
    Complete GO annotation...

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein O-GlcNAcase1 Publication (EC:3.2.1.1691 Publication)
    Short name:
    OGA1 Publication
    Alternative name(s):
    Beta-N-acetylglucosaminidase (EC:3.2.1.521 Publication)
    Beta-N-acetylhexosaminidase
    Beta-hexosaminidase
    N-acetyl-beta-D-glucosaminidase
    N-acetyl-beta-glucosaminidase
    Gene namesi
    ORF Names:OG2516_04129Imported
    OrganismiOceanicola granulosus (strain ATCC BAA-861 / DSM 15982 / KCTC 12143 / HTCC2516)Imported
    Taxonomic identifieri314256 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeOceanicola
    Proteomesi
    • UP000003635 Componenti: Unassembled WGS sequence

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 447447Protein O-GlcNAcasePRO_0000430934Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi314256.OG2516_04129.

    Structurei

    Secondary structure

    1
    447
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi2 – 76Combined sources
    Beta strandi10 – 123Combined sources
    Helixi16 – 2813Combined sources
    Beta strandi33 – 364Combined sources
    Turni42 – 498Combined sources
    Helixi54 – 6815Combined sources
    Turni69 – 713Combined sources
    Beta strandi73 – 786Combined sources
    Helixi89 – 10416Combined sources
    Beta strandi109 – 1135Combined sources
    Turni123 – 1286Combined sources
    Helixi132 – 14615Combined sources
    Turni147 – 1493Combined sources
    Beta strandi150 – 1567Combined sources
    Helixi162 – 1654Combined sources
    Helixi169 – 1713Combined sources
    Helixi173 – 1819Combined sources
    Beta strandi186 – 1905Combined sources
    Beta strandi193 – 1964Combined sources
    Helixi202 – 21211Combined sources
    Beta strandi217 – 2204Combined sources
    Helixi226 – 2283Combined sources
    Helixi242 – 2476Combined sources
    Beta strandi249 – 2535Combined sources
    Helixi259 – 2624Combined sources
    Helixi263 – 27412Combined sources
    Helixi280 – 29112Combined sources
    Helixi292 – 2954Combined sources
    Helixi306 – 31510Combined sources
    Helixi324 – 33613Combined sources
    Beta strandi338 – 3414Combined sources
    Helixi347 – 37125Combined sources
    Helixi376 – 40429Combined sources
    Helixi427 – 4348Combined sources
    Beta strandi435 – 4373Combined sources

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2XSAX-ray2.00A1-447[»]
    2XSBX-ray2.11A1-447[»]
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ2CEE3.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni219 – 2213Substrate bindingImported

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 84 family.Curated

    Phylogenomic databases

    eggNOGiENOG4105CPE. Bacteria.
    ENOG410XPBQ. LUCA.

    Family and domain databases

    InterProiIPR011496. Beta-N-acetylglucosaminidase.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF07555. NAGidase. 1 hit.
    [Graphical view]
    SUPFAMiSSF51445. SSF51445. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q2CEE3-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MLTGVIEGFY GRDWRRDERA TVMDWIAAAG MNTYIYGPKD DVHVRARWRV
    60 70 80 90 100
    PYDAAGLARL TELRDAAAAR GMVFYVSLAP CLDVTYSDPQ DRAALLARVD
    110 120 130 140 150
    QLARAGLRNL VLLFDDIPSV LPEADRHRFD SFAEAQADLS NMVLRHLRGA
    160 170 180 190 200
    GHVVFCPTEY CGRMAGGDPR GSAYLQRLGS TLDPAIDIFW TGPEIVSEEI
    210 220 230 240 250
    VAAHLAAVGE VLRRRPVIWD NFHANDYDIR RVFAGPLGGR SRDILPLVAG
    260 270 280 290 300
    WITNPNNEAE ANFPAIHTTG AYLADPDYAP ERAIAAAVAA WQPRFRLAFG
    310 320 330 340 350
    DGAVPSDLVA LLCDLFWQPF ALGPETTRIL SALRAALTVP RPDPSDPAWR
    360 370 380 390 400
    AALEDLRDLK RRINKLFTLM TEIENRDLFH TFHNYLWEAQ EEVGHLVAYC
    410 420 430 440
    DWLDEAPPPG AVFPATDRIH NFYRRGFGVA VQDILQRDRQ GRYHHGV
    Length:447
    Mass (Da):50,038
    Last modified:April 4, 2006 - v1
    Checksum:iB06BDD37C16C2F94
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AAOT01000018 Genomic DNA. Translation: EAR51054.1.
    RefSeqiWP_007254354.1. NZ_CH724107.1.

    Genome annotation databases

    EnsemblBacteriaiEAR51054; EAR51054; OG2516_04129.
    PATRICi28498282. VBIOceGra115923_0282.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AAOT01000018 Genomic DNA. Translation: EAR51054.1.
    RefSeqiWP_007254354.1. NZ_CH724107.1.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2XSAX-ray2.00A1-447[»]
    2XSBX-ray2.11A1-447[»]
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi314256.OG2516_04129.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiEAR51054; EAR51054; OG2516_04129.
    PATRICi28498282. VBIOceGra115923_0282.

    Phylogenomic databases

    eggNOGiENOG4105CPE. Bacteria.
    ENOG410XPBQ. LUCA.

    Miscellaneous databases

    EvolutionaryTraceiQ2CEE3.

    Family and domain databases

    InterProiIPR011496. Beta-N-acetylglucosaminidase.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF07555. NAGidase. 1 hit.
    [Graphical view]
    SUPFAMiSSF51445. SSF51445. 1 hit.
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. "Genome sequences of Oceanicola granulosus HTCC2516(T) and Oceanicola batsensis HTCC2597(TDelta)."
      Thrash J.C., Cho J.C., Vergin K.L., Giovannoni S.J.
      J. Bacteriol. 192:3549-3550(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC BAA-861 / DSM 15982 / KCTC 12143 / HTCC2516Imported.
    2. "Human OGA binds substrates in a conserved peptide recognition groove."
      Schimpl M., Schuttelkopf A.W., Borodkin V.S., van Aalten D.M.
      Biochem. J. 432:1-7(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS) OF APOPROTEIN AND IN COMPLEX WITH THE SUBSTRATE ANALOG PUGNAC, FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, ENZYME REGULATION.
      Strain: ATCC BAA-861 / DSM 15982 / KCTC 12143 / HTCC25161 Publication.

    Entry informationi

    Entry nameiOGA_OCEGH
    AccessioniPrimary (citable) accession number: Q2CEE3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 26, 2014
    Last sequence update: April 4, 2006
    Last modified: March 16, 2016
    This is version 33 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.