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Protein

Thymidylate synthase

Gene

thyA

Organism
Francisella tularensis subsp. holarctica (strain LVS)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Provides the sole de novo source of dTMP for DNA biosynthesis.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.UniRule annotation

Pathway: dTTP biosynthesis

This protein is involved in the pathway dTTP biosynthesis, which is part of Pyrimidine metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway dTTP biosynthesis and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei156 – 1561UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Nucleotide biosynthesis

Enzyme and pathway databases

BioCyciFTUL376619:GI22-741-MONOMER.
UniPathwayiUPA00575.

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidylate synthaseUniRule annotation (EC:2.1.1.45UniRule annotation)
Short name:
TSUniRule annotation
Short name:
TSaseUniRule annotation
Gene namesi
Name:thyAUniRule annotation
Ordered Locus Names:FTL_0715
OrganismiFrancisella tularensis subsp. holarctica (strain LVS)
Taxonomic identifieri376619 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaThiotrichalesFrancisellaceaeFrancisella

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 274274Thymidylate synthasePRO_1000000599Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi376619.FTL_0715.

Structurei

3D structure databases

ProteinModelPortaliQ2A486.
SMRiQ2A486. Positions 2-274.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0207.
HOGENOMiHOG000257899.
KOiK00560.
OMAiNEWADEN.
OrthoDBiEOG6K6V53.

Family and domain databases

Gene3Di3.30.572.10. 1 hit.
HAMAPiMF_00008. Thymidy_synth_bact.
InterProiIPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
[Graphical view]
PfamiPF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PRINTSiPR00108. THYMDSNTHASE.
SUPFAMiSSF55831. SSF55831. 1 hit.
TIGRFAMsiTIGR03284. thym_sym. 2 hits.

Sequencei

Sequence statusi: Complete.

Q2A486-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MREYLNFLKY IKENGVLKND RTGTGTRSIF GYQMRFDLQK GFPLVTTKKI
60 70 80 90 100
HIPSVVHELL WFLSGSTNIK YLNDNNVRIW NEWATVDGEL GPIYGKQWRD
110 120 130 140 150
FNGQGIDQIA DVIQMLKTNP NSRRILVLAW NPCVVPSEKI SPQENVVKGN
160 170 180 190 200
SALPPCHAMF QFYVANNKLS CMLTQRSADA FLGVPFNIAS YSLLTHMVAQ
210 220 230 240 250
QCNLDVGELI WSGGDCHIYN NHIEQVNEQL SREPLALPTL KILRKSNSIF
260 270
DYKYEDFEFE NYNHHPAIKA KISV
Length:274
Mass (Da):31,415
Last modified:April 4, 2006 - v1
Checksum:i2414889305960F98
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM233362 Genomic DNA. Translation: CAJ79154.1.
RefSeqiWP_003015196.1. NC_007880.1.
YP_513466.1. NC_007880.1.

Genome annotation databases

EnsemblBacteriaiCAJ79154; CAJ79154; FTL_0715.
KEGGiftl:FTL_0715.
PATRICi17942443. VBIFraTul90181_0781.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM233362 Genomic DNA. Translation: CAJ79154.1.
RefSeqiWP_003015196.1. NC_007880.1.
YP_513466.1. NC_007880.1.

3D structure databases

ProteinModelPortaliQ2A486.
SMRiQ2A486. Positions 2-274.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi376619.FTL_0715.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAJ79154; CAJ79154; FTL_0715.
KEGGiftl:FTL_0715.
PATRICi17942443. VBIFraTul90181_0781.

Phylogenomic databases

eggNOGiCOG0207.
HOGENOMiHOG000257899.
KOiK00560.
OMAiNEWADEN.
OrthoDBiEOG6K6V53.

Enzyme and pathway databases

UniPathwayiUPA00575.
BioCyciFTUL376619:GI22-741-MONOMER.

Family and domain databases

Gene3Di3.30.572.10. 1 hit.
HAMAPiMF_00008. Thymidy_synth_bact.
InterProiIPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
[Graphical view]
PfamiPF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PRINTSiPR00108. THYMDSNTHASE.
SUPFAMiSSF55831. SSF55831. 1 hit.
TIGRFAMsiTIGR03284. thym_sym. 2 hits.
ProtoNetiSearch...

Publicationsi

  1. "Complete genome sequence of Francisella tularensis LVS (Live Vaccine Strain)."
    Chain P., Larimer F., Land M., Stilwagen S., Larsson P., Bearden S., Chu M., Oyston P., Forsman M., Andersson S., Lindler L., Titball R., Garcia E.
    Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: LVS.

Entry informationi

Entry nameiTYSY_FRATH
AccessioniPrimary (citable) accession number: Q2A486
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: April 4, 2006
Last modified: June 24, 2015
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.