Q2A2S0 (PYRG_FRATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
CTP synthase
EC=6.3.4.2

Alternative name(s):
CTP synthetase
UTP--ammonia ligase

Gene names

Name:	pyrG
Ordered Locus Names:	FTL_1311

Organism

Francisella tularensis subsp. holarctica (strain LVS) [Complete proteome] [HAMAP]

Taxonomic identifier

376619 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Thiotrichales › Francisellaceae › Francisella › Francisella tularensis › Francisella tularensis subsp. holarctica

Protein attributes

Sequence length	546 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen By similarity. HAMAP-Rule MF_01227
Catalytic activity	ATP + UTP + NH3 = ADP + phosphate + CTP. HAMAP-Rule MF_01227
Enzyme regulation	Allosterically activated by GTP, when glutamine is the substrate. Inhibited by CTP By similarity. HAMAP-Rule MF_01227
Pathway	Pyrimidine metabolism; CTP biosynthesis via de novo pathway; CTP from UDP: step 2/2. HAMAP-Rule MF_01227
Subunit structure	Homotetramer By similarity. HAMAP-Rule MF_01227
Sequence similarities	Belongs to the CTP synthase family. Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	'de novo' CTP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway glutamine metabolic process Inferred from electronic annotation. Source: HAMAP
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW CTP synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 546	546	CTP synthase HAMAP-Rule MF_01227		PRO_0000266119
Regions
	Domain	294 – 546	253	Glutamine amidotransferase type-1
	Region	1 – 256	256	Aminator domain HAMAP-Rule MF_01227
Sites
	Active site	383	1	Nucleophile By similarity
	Active site	519	1	By similarity
	Active site	521	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2A2S0 [UniParc]. Last modified April 4, 2006. Version 1. Checksum: 60D0C7DF40286E9D	FASTA	546	61,042
	10 20 30 40 50 60 MNSNTKIIFV TGGVVSSLGK GVTAASLATL LESRGLNVTM MKLDPYINVD PGTMSPLQHG 70 80 90 100 110 120 EVFVTEDGAE TDLDLGHYER FIRNKMTQAN NFTTGKVYQS VLRRERKGDY LGATIQVIPH 130 140 150 160 170 180 ITDEIKRRIC SGIADDVDVA IVEIGGTVGD IESQPFLEAI RQLRIELGRN RTLFVHLTLL 190 200 210 220 230 240 PYIKVAGEIK TKPTQHSVKE LRGIGIQADV LVCRCEKKFD DSEKRKIALF TNVDQDCIFT 250 260 270 280 290 300 AEDVDTIYEV PLKYNQQGFD AKLVELLNLN AKEADLSEWQ NVVNTIRDVK GEVIIAMVGK 310 320 330 340 350 360 YVSLTEAYKS LNEALYNAGY KKGVKVKIKF VDSEDVNENN VESYFKDVAA ILVPGGFGSR 370 380 390 400 410 420 GVEGKIISIK YARENQIPFL GICLGMQLAV IEYARNILGI KDAHSSELEP TTANPVIGLI 430 440 450 460 470 480 TEWQAEDGTV HQRTHSSDLG GTMRLGGYKC VLKQGSRARE IYQADEVVER HRHRYEVNSN 490 500 510 520 530 540 YVERLEEAGL IFSGRSEDNK LMELIEIPQH KWFIACQAHP EFTSTPRYGH KLFESYIQAA IENSNN

References

[1]

"Complete genome sequence of Francisella tularensis LVS (Live Vaccine Strain)." Chain P., Larimer F., Land M., Stilwagen S., Larsson P., Bearden S., Chu M., Oyston P., Forsman M., Andersson S., Lindler L., Titball R., Garcia E. Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: LVS.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM233362 Genomic DNA. Translation: CAJ79750.1.
RefSeq	YP_513982.1. NC_007880.1.
3D structure databases
ProteinModelPortal	Q2A2S0.
SMR	Q2A2S0. Positions 4-545.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAJ79750; CAJ79750; FTL_1311.
GeneID	3952302.
KEGG	ftl:FTL_1311.
PATRIC	17943756. VBIFraTul90181_1427.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0504.
HOGENOM	HOG000077515.
KO	K01937.
OMA	TNEIKDR.
ProtClustDB	PRK05380.
Enzyme and pathway databases
BioCyc	FTUL376619:GI22-1344-MONOMER.
UniPathway	UPA00159; UER00277.
Family and domain databases
HAMAP	MF_01227. PyrG.
InterPro	IPR004468. CTP_synthase. IPR017456. CTP_synthase_N. IPR017926. GATASE. IPR027417. P-loop_NTPase. [Graphical view]
PANTHER	PTHR11550. PTHR11550. 1 hit.
Pfam	PF06418. CTP_synth_N. 1 hit. PF00117. GATase. 1 hit. [Graphical view]
SUPFAM	SSF52540. SSF52540. 1 hit.
TIGRFAMs	TIGR00337. PyrG. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRG_FRATH

Accession

Primary (citable) accession number: Q2A2S0

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 12, 2006
Last sequence update:	April 4, 2006
Last modified:	May 29, 2013
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program