ID   ACON_FRATH              Reviewed;         937 AA.
AC   Q2A1K3;
DT   16-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT   04-APR-2006, sequence version 1.
DT   16-JUN-2009, entry version 23.
DE   RecName: Full=Aconitate hydratase;
DE            Short=Aconitase;
DE            EC=4.2.1.3;
DE   AltName: Full=Citrate hydro-lyase;
GN   Name=acn; OrderedLocusNames=FTL_1772;
OS   Francisella tularensis subsp. holarctica (strain LVS).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Thiotrichales;
OC   Francisellaceae; Francisella.
OX   NCBI_TaxID=376619;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Chain P., Larimer F., Land M., Stilwagen S., Larsson P., Bearden S.,
RA   Chu M., Oyston P., Forsman M., Andersson S., Lindler L., Titball R.,
RA   Garcia E.;
RT   "Complete genome sequence of Francisella tularensis LVS (Live Vaccine
RT   Strain).";
RL   Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY: Citrate = isocitrate.
CC   -!- COFACTOR: Binds 1 4Fe-4S cluster per subunit (By similarity).
CC   -!- PATHWAY: Carbohydrate metabolism; tricarboxylic acid cycle.
CC   -!- SIMILARITY: Belongs to the aconitase/IPM isomerase family.
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DR   EMBL; AM233362; CAJ80211.1; -; Genomic_DNA.
DR   RefSeq; YP_514399.1; -.
DR   GeneID; 3951896; -.
DR   GenomeReviews; AM233362_GR; FTL_1772.
DR   KEGG; ftl:FTL_1772; -.
DR   HOGENOM; Q2A1K3; -.
DR   OMA; Q2A1K3; RIKNEML.
DR   BioCyc; FTUL351581:FTL_1772-MON; -.
DR   GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0003994; F:aconitate hydratase activity; IEA:EC.
DR   GO; GO:0005506; F:iron ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-KW.
DR   InterPro; IPR015931; Acnase/IPM_dHydase_lsu_aba_1/3.
DR   InterPro; IPR001030; Acoase/IPM_deHydtase_lsu_aba.
DR   InterPro; IPR015937; Aconitase-like_core.
DR   InterPro; IPR015928; Aconitase/3IPM_dehydase_swvl.
DR   InterPro; IPR006249; Aconitase/Fe_reg_prot_2.
DR   InterPro; IPR015934; Aconitase/Fe_reg_prot_2/AcnD.
DR   InterPro; IPR015932; Aconitase/IPMdHydase_lsu_aba_2.
DR   InterPro; IPR018136; Aconitase_4Fe-4S_BS.
DR   InterPro; IPR000573; AconitaseA/IPMdHydase_ssu_swvl.
DR   Gene3D; G3DSA:3.30.499.10; Acnase/IPM_dHydase_lsu_aba_1/3; 2.
DR   Gene3D; G3DSA:3.20.19.10; Aconitase/3IPM_dehydase_swvl; 1.
DR   Gene3D; G3DSA:3.40.1060.10; Aconitase/IPMdHydase_lsu_aba_2; 1.
DR   PANTHER; PTHR11670; Aconitase-like_core; 1.
DR   PANTHER; PTHR11670:SF1; Aconitase/Fe_reg_prot_2/AcnD; 1.
DR   Pfam; PF00330; Aconitase; 1.
DR   Pfam; PF00694; Aconitase_C; 1.
DR   PRINTS; PR00415; ACONITASE.
DR   ProDom; PD000511; Aconitase_N; 1.
DR   TIGRFAMs; TIGR01341; aconitase_1; 1.
DR   PROSITE; PS00450; ACONITASE_1; 1.
DR   PROSITE; PS01244; ACONITASE_2; 1.
PE   3: Inferred from homology;
KW   4Fe-4S; Complete proteome; Iron; Iron-sulfur; Lyase; Metal-binding;
KW   Tricarboxylic acid cycle.
FT   CHAIN         1    937       Aconitate hydratase.
FT                                /FTId=PRO_0000235166.
FT   METAL       439    439       Iron-sulfur (4Fe-4S) (By similarity).
FT   METAL       505    505       Iron-sulfur (4Fe-4S) (By similarity).
FT   METAL       508    508       Iron-sulfur (4Fe-4S) (By similarity).
SQ   SEQUENCE   937 AA;  102704 MW;  3784B39F6F6980C9 CRC64;
     MSDIKNITKL QIEDKGKKYS LYSLKKLSQE LGKDVTRLPY SIRVLLENQL RNIDGYKVKE
     DDMHKVLDWD AKASSRPEIP HMPARVVMQD FTGVPAVVDL AAMRKAIKDA GGDADKINPL
     VDTAMVIDHS VQVDFYGTKT ALAQNVAKEF ERNGERYSLL KWAQKAFDDF IVVPPGMGII
     HQVNLEYLAK DALVKNINGE DVIYPDTLVG TDSHTTMING VGAVGWGVGG IEAEAVMLGQ
     PYYMVLPDVV GVKFTGKLKT GVTATDLVLK VTEVLRKHGV VGKFVEYYGE GLESLSLPDR
     ATIANMTPEY GATIGFFPVD EVTLDFFNNT NRSELVDAAR EMYKEQLLFR ENPAEEPEYS
     NIVEIDLSEV ESNLAGPKRP QDRVAFHDMK KAFAEALVHE QGLHGFGLTD EQLQKSAEVK
     GLNERITHGS VAIAAITSCT NTSNPSLLLG AGLLAKKANE KGLKVKPFVK TSLAPGSQVV
     TQYLEKANLL PELENLGFNL VGYGCTTCIG NSGPLDEPVV EAINEADLIV ASVSSGNRNF
     EGRINPHIKA NYLASPIHVV AYALAGTVDF DPVEDAIGKD AEGNDVYLAD IWPTTEEIAA
     IQSHVINSDM FKKAYATVLD GTEEWQKLKA PEGKLYEFDS SSTYIQCPNF FEKFAEGNDD
     LDIKGARTLL MLGDSVTTDH ISPAGAIPEE YPAGQYLKSH GVEKKDFNSY GSRRGNHEVM
     MRGTFANIRI RNLLLDNVEG GFTKYHLDGS QQYVFDAAMK YKEKGIPLVI LAGKEYGTGS
     SRDWAAKGTF LLGVKAVIAE SYERIHRSNL VGMGVLPLEY VNGQNAKTLG LDGTEMFNIK
     NLNNIKPRQI VIVEAVHPKT AHTTTFEALA RLDADVDVDY LKNGGILQTV LKDIMGDKKE
     SKSTQSTTSK GCGSADTSSE TSCPFAKIAN FFKKLFK
//