Q2A1K3 (ACON_FRATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aconitate hydratase
Short name=Aconitase
EC=4.2.1.3

Alternative name(s):
Citrate hydro-lyase

Gene names

Name:	acn
Ordered Locus Names:	FTL_1772

Organism

Francisella tularensis subsp. holarctica (strain LVS) [Complete proteome] [HAMAP]

Taxonomic identifier

376619 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Thiotrichales › Francisellaceae › Francisella

Protein attributes

Sequence length	937 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the isomerization of citrate to isocitrate via cis-aconitate By similarity.
Catalytic activity	Citrate = isocitrate.
Cofactor	Binds 1 4Fe-4S cluster per subunit By similarity.
Pathway	Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate from oxaloacetate: step 2/2.
Sequence similarities	Belongs to the aconitase/IPM isomerase family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW aconitate hydratase activity Inferred from electronic annotation. Source: EC citrate hydro-lyase (cis-aconitate-forming) activity Inferred from electronic annotation. Source: EC isocitrate hydro-lyase (cis-aconitate-forming) activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 937	937	Aconitate hydratase		PRO_0000235166
Sites
	Metal binding	439	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	505	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	508	1	Iron-sulfur (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2A1K3 [UniParc]. Last modified April 4, 2006. Version 1. Checksum: 3784B39F6F6980C9	FASTA	937	102,704
	10 20 30 40 50 60 MSDIKNITKL QIEDKGKKYS LYSLKKLSQE LGKDVTRLPY SIRVLLENQL RNIDGYKVKE 70 80 90 100 110 120 DDMHKVLDWD AKASSRPEIP HMPARVVMQD FTGVPAVVDL AAMRKAIKDA GGDADKINPL 130 140 150 160 170 180 VDTAMVIDHS VQVDFYGTKT ALAQNVAKEF ERNGERYSLL KWAQKAFDDF IVVPPGMGII 190 200 210 220 230 240 HQVNLEYLAK DALVKNINGE DVIYPDTLVG TDSHTTMING VGAVGWGVGG IEAEAVMLGQ 250 260 270 280 290 300 PYYMVLPDVV GVKFTGKLKT GVTATDLVLK VTEVLRKHGV VGKFVEYYGE GLESLSLPDR 310 320 330 340 350 360 ATIANMTPEY GATIGFFPVD EVTLDFFNNT NRSELVDAAR EMYKEQLLFR ENPAEEPEYS 370 380 390 400 410 420 NIVEIDLSEV ESNLAGPKRP QDRVAFHDMK KAFAEALVHE QGLHGFGLTD EQLQKSAEVK 430 440 450 460 470 480 GLNERITHGS VAIAAITSCT NTSNPSLLLG AGLLAKKANE KGLKVKPFVK TSLAPGSQVV 490 500 510 520 530 540 TQYLEKANLL PELENLGFNL VGYGCTTCIG NSGPLDEPVV EAINEADLIV ASVSSGNRNF 550 560 570 580 590 600 EGRINPHIKA NYLASPIHVV AYALAGTVDF DPVEDAIGKD AEGNDVYLAD IWPTTEEIAA 610 620 630 640 650 660 IQSHVINSDM FKKAYATVLD GTEEWQKLKA PEGKLYEFDS SSTYIQCPNF FEKFAEGNDD 670 680 690 700 710 720 LDIKGARTLL MLGDSVTTDH ISPAGAIPEE YPAGQYLKSH GVEKKDFNSY GSRRGNHEVM 730 740 750 760 770 780 MRGTFANIRI RNLLLDNVEG GFTKYHLDGS QQYVFDAAMK YKEKGIPLVI LAGKEYGTGS 790 800 810 820 830 840 SRDWAAKGTF LLGVKAVIAE SYERIHRSNL VGMGVLPLEY VNGQNAKTLG LDGTEMFNIK 850 860 870 880 890 900 NLNNIKPRQI VIVEAVHPKT AHTTTFEALA RLDADVDVDY LKNGGILQTV LKDIMGDKKE 910 920 930 SKSTQSTTSK GCGSADTSSE TSCPFAKIAN FFKKLFK

References

[1]

"Complete genome sequence of Francisella tularensis LVS (Live Vaccine Strain)." Chain P., Larimer F., Land M., Stilwagen S., Larsson P., Bearden S., Chu M., Oyston P., Forsman M., Andersson S., Lindler L., Titball R., Garcia E. Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: LVS.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM233362 Genomic DNA. Translation: CAJ80211.1.
RefSeq	YP_514399.1. NC_007880.1.
3D structure databases
ProteinModelPortal	Q2A1K3.
SMR	Q2A1K3. Positions 8-895.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3951896.
GenomeReviews	Gene locus FTL_1772 in contig AM233362_GR.
KEGG	ftl:FTL_1772.
PATRIC	17944787. VBIFraTul90181_1927.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG289738.
OMA	YSKAQGM.
PhylomeDB	Q2A1K3.
ProtClustDB	PRK09277.
Enzyme and pathway databases
BioCyc	FTUL351581:FTL_1772-MONOMER.
Family and domain databases
InterPro	IPR015931. Acnase/IPM_dHydase_lsu_aba_1/3. IPR015937. Acoase/IPM_deHydtase. IPR001030. Acoase/IPM_deHydtase_lsu_aba. IPR015928. Aconitase/3IPM_dehydase_swvl. IPR006249. Aconitase/Fe_reg_prot_2. IPR015934. Aconitase/Fe_reg_prot_2/AcnD. IPR015932. Aconitase/IPMdHydase_lsu_aba_2. IPR018136. Aconitase_4Fe-4S_BS. IPR000573. AconitaseA/IPMdHydase_ssu_swvl. [Graphical view]
Gene3D	G3DSA:3.30.499.10. Acnase/IPM_dHydase_lsu_aba_1/3. 2 hits. G3DSA:3.20.19.10. Aconitase/3IPM_dehydase_swvl. 1 hit. G3DSA:3.40.1060.10. Aconitase/IPMdHydase_lsu_aba_2. 1 hit.
KO	K01681.
PANTHER	PTHR11670. Aconitase-like_core. 1 hit. PTHR11670:SF1. Aconitase/Fe_reg_prot_2/AcnD. 1 hit.
Pfam	PF00330. Aconitase. 1 hit. PF00694. Aconitase_C. 1 hit. [Graphical view]
PRINTS	PR00415. ACONITASE.
SUPFAM	SSF52016. Aconitase/3IPM_dehydase_swvl. 1 hit. SSF53732. Aconitase_N. 1 hit.
TIGRFAMs	TIGR01341. Aconitase_1. 1 hit.
PROSITE	PS00450. ACONITASE_1. 1 hit. PS01244. ACONITASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ACON_FRATH

Accession

Primary (citable) accession number: Q2A1K3

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 16, 2006
Last sequence update:	April 4, 2006
Last modified:	January 25, 2012
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program