Reviewed, UniProtKB/Swiss-Prot Q29ZJ1 (LACG_LACRH)

Names and origin

Protein names

Recommended name:
    6-phospho-beta-galactosidase
    EC=3.2.1.85
Alternative name(s):
    Beta-D-phosphogalactoside galactohydrolase
      Short name=PGALase
    P-beta-Gal
      Short name=PBG

Gene names

Name:

lacG

Organism

Lactobacillus rhamnosus

Taxonomic identifier

47715 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Lactobacillaceae › Lactobacillus

Protein attributes

Sequence length	474 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	A 6-phospho-beta-D-galactoside + H2O = 6-phospho-D-galactose + an alcohol. HAMAP MF_01574
Pathway	Carbohydrate metabolism; lactose degradation; D-galactose 6-phosphate and beta-D-glucose from lactose 6-phosphate: step 1/1. HAMAP MF_01574
Sequence similarities	Belongs to the glycosyl hydrolase 1 family.

Ontologies

Keywords
Molecular function	Glycosidase Hydrolase
Gene Ontology (GO)
Biological process	lactose catabolic process via tagatose-6-phosphate Inferred from electronic annotation. Source: InterPro
Molecular function	6-phospho-beta-galactosidase activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro galactosidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 474	474	6-phospho-beta-galactosidase HAMAP MF_01574		PRO_0000260723
Sites
	Active site	160	1	Proton donor By similarity
	Active site	375	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q29ZJ1-1 [UniParc]. Last modified April 4, 2006. Version 1. Checksum: E115E26364815405	FASTA	474	53,727
	10 20 30 40 50 60 MRKQLPKDFV IGGATAAYQV EGATKEDGKG RVLWDDFLEK QGRFSPDPAA DFYHRYDEDL 70 80 90 100 110 120 ALAEAYGHQV IRLSIAWSRI FPDGAGAVEP RGVAFYHRLF AACAKHHLIP FVTLHHFDTP 130 140 150 160 170 180 ERLHAIGDWL SQEMLEDFVE YARFCFEEFP EIKHWITINE PTSMAVQQYT SGTFPPAETG 190 200 210 220 230 240 HFDKTFQAEH NQIVAHARIV NLYKSMGLDG EIGIVHALQT PYPYSDSSED QHAADLQDAL 250 260 270 280 290 300 ENRLYLDGTL AGDYAPKTLA LIKEILAANQ QPMFKYTDEE MAAIKKAAHQ LDFVGVNNYF 310 320 330 340 350 360 SKWLRAYHGK SETIHNGDGS KGSSVARLHG IGEEKKPAGI ETTDWDWSIY PRGMYDMLMR 370 380 390 400 410 420 IHQDYPLVPA IYVTENGIGL KESLPAEVTP NTVIADPKRI DYLKKYLSAV ADAIQAGANV 430 440 450 460 470 KGYFVWSLQD QFSWTNGYSK RYGLFFVDFP TQKRYVKQSA EWLKQVSQTH VIPE

References

[1]	"Sequence, organization, transcription and regulation of lactose and galactose operons in Lactobacillus rhamnosus TCELL-1." Tsai Y.-K., Lin T.-H. J. Appl. Microbiol. 100:446-459(2006) [PubMed: 16478484] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: TCELL-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY705915 Genomic DNA. Translation: AAW30155.1.
3D structure databases
SMR	Q29ZJ1. Positions 1-472.
ModBase	Search...
Enzyme and pathway databases
BRENDA	3.2.1.85. 141488.
Family and domain databases
HAMAP	MF_01574. LacG. [Tree]
InterPro	IPR001360. Glyco_hydro_1. IPR018120. Glyco_hydro_1_AS. IPR017853. Glyco_hydro_catalytic_core. IPR013781. Glyco_hydro_sg_catalytic. IPR005928. LacG. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
PANTHER	PTHR10353. Glyco_hydro_1. 1 hit.
Pfam	PF00232. Glyco_hydro_1. 1 hit. [Graphical view]
PRINTS	PR00131. GLHYDRLASE1.
TIGRFAMs	TIGR01233. lacG. 1 hit.
PROSITE	PS00572. GLYCOSYL_HYDROL_F1_1. 1 hit. PS00653. GLYCOSYL_HYDROL_F1_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LACG_LACRH

Accession

Primary (citable) accession number: Q29ZJ1

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2006
Last sequence update:	April 4, 2006
Last modified:	February 9, 2010
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)