ID   Q29ST6_DANRE            Unreviewed;       442 AA.
AC   Q29ST6; B3DJ59;
DT   04-APR-2006, integrated into UniProtKB/TrEMBL.
DT   04-APR-2006, sequence version 1.
DT   27-MAR-2024, entry version 137.
DE   RecName: Full=Adenosine receptor A2 {ECO:0000256|RuleBase:RU201114};
GN   Name=adora2aa {ECO:0000313|Ensembl:ENSDARP00000121566,
GN   ECO:0000313|RefSeq:NP_001034904.1, ECO:0000313|RefSeq:XP_005167233.1,
GN   ECO:0000313|ZFIN:ZDB-GENE-080723-28};
GN   Synonyms=a2aa {ECO:0000313|RefSeq:NP_001034904.1}, adora2a.1
GN   {ECO:0000313|EMBL:AAX63345.1, ECO:0000313|RefSeq:XP_005167233.1};
GN   ORFNames=zgc:194773 {ECO:0000313|EMBL:AAI63368.1,
GN   ECO:0000313|RefSeq:NP_001034904.1, ECO:0000313|RefSeq:XP_005167233.1};
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955 {ECO:0000313|EMBL:AAI63368.1};
RN   [1] {ECO:0000313|EMBL:AAX63345.1}
RP   NUCLEOTIDE SEQUENCE.
RA   Boehmler W.A., Woll M.P., Canfield V.A., Levenson R.;
RL   Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases.
RN   [2] {ECO:0000313|EMBL:AAI63368.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RG   NIH - Zebrafish Gene Collection (ZGC) project;
RL   Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases.
RN   [3] {ECO:0000313|EMBL:AAX63345.1, ECO:0000313|RefSeq:NP_001034904.1}
RP   NUCLEOTIDE SEQUENCE.
RX   PubMed=19070682; DOI=10.1016/j.gep.2008.11.006;
RA   Boehmler W., Petko J., Woll M., Frey C., Thisse B., Thisse C.,
RA   Canfield V.A., Levenson R.;
RT   "Identification of zebrafish A2 adenosine receptors and expression in
RT   developing embryos.";
RL   Gene Expr. Patterns 9:144-151(2009).
RN   [4] {ECO:0000313|RefSeq:NP_001034904.1}
RP   NUCLEOTIDE SEQUENCE.
RX   PubMed=21496211;
RA   Maximino C., Lima M.G., Olivera K.R., Picanco-Diniz D.L., Herculano A.M.;
RT   "Adenosine A1, but not A2, receptor blockade increases anxiety and arousal
RT   in Zebrafish.";
RL   Basic Clin. Pharmacol. Toxicol. 109:203-207(2011).
RN   [5] {ECO:0000313|RefSeq:NP_001034904.1}
RP   NUCLEOTIDE SEQUENCE.
RX   PubMed=21603935;
RA   Piato A.L., Rosemberg D.B., Capiotti K.M., Siebel A.M., Herrmann A.P.,
RA   Ghisleni G., Vianna M.R., Bogo M.R., Lara D.R., Bonan C.D.;
RT   "Acute restraint stress in zebrafish: behavioral parameters and purinergic
RT   signaling.";
RL   Neurochem. Res. 36:1876-1886(2011).
RN   [6] {ECO:0000313|RefSeq:NP_001034904.1}
RP   NUCLEOTIDE SEQUENCE.
RX   PubMed=21914471; DOI=10.1016/j.ntt.2011.08.010;
RA   Capiotti K.M., Menezes F.P., Nazario L.R., Pohlmann J.B., de Oliveira G.M.,
RA   Fazenda L., Bogo M.R., Bonan C.D., Da Silva R.S.;
RT   "Early exposure to caffeine affects gene expression of adenosine receptors,
RT   DARPP-32 and BDNF without affecting sensibility and morphology of
RT   developing zebrafish (Danio rerio).";
RL   Neurotoxicol. Teratol. 33:680-685(2011).
RN   [7] {ECO:0000313|RefSeq:NP_001034904.1}
RP   NUCLEOTIDE SEQUENCE.
RX   PubMed=22608007;
RA   Andersson O., Adams B.A., Yoo D., Ellis G.C., Gut P., Anderson R.M.,
RA   German M.S., Stainier D.Y.;
RT   "Adenosine signaling promotes regeneration of pancreatic beta cells in
RT   vivo.";
RL   Cell Metab. 15:885-894(2012).
RN   [8] {ECO:0000313|Ensembl:ENSDARP00000121566}
RP   IDENTIFICATION.
RC   STRAIN=Tuebingen {ECO:0000313|Ensembl:ENSDARP00000121566};
RG   Ensembl;
RL   Submitted (FEB-2012) to UniProtKB.
RN   [9] {ECO:0000313|Ensembl:ENSDARP00000121566, ECO:0000313|Proteomes:UP000000437}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Tuebingen {ECO:0000313|Ensembl:ENSDARP00000121566};
RX   PubMed=23594743; DOI=10.1038/nature12111;
RG   Genome Reference Consortium Zebrafish;
RA   Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA   Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA   Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA   White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA   Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA   Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA   Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA   Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA   Clee C., Oliver K., Clark R., Riddle C., Elliot D., Eliott D.,
RA   Threadgold G., Harden G., Ware D., Begum S., Mortimore B., Mortimer B.,
RA   Kerry G., Heath P., Phillimore B., Tracey A., Corby N., Dunn M.,
RA   Johnson C., Wood J., Clark S., Pelan S., Griffiths G., Smith M.,
RA   Glithero R., Howden P., Barker N., Lloyd C., Stevens C., Harley J.,
RA   Holt K., Panagiotidis G., Lovell J., Beasley H., Henderson C., Gordon D.,
RA   Auger K., Wright D., Collins J., Raisen C., Dyer L., Leung K.,
RA   Robertson L., Ambridge K., Leongamornlert D., McGuire S., Gilderthorp R.,
RA   Griffiths C., Manthravadi D., Nichol S., Barker G., Whitehead S., Kay M.,
RA   Brown J., Murnane C., Gray E., Humphries M., Sycamore N., Barker D.,
RA   Saunders D., Wallis J., Babbage A., Hammond S., Mashreghi-Mohammadi M.,
RA   Barr L., Martin S., Wray P., Ellington A., Matthews N., Ellwood M.,
RA   Woodmansey R., Clark G., Cooper J., Cooper J., Tromans A., Grafham D.,
RA   Skuce C., Pandian R., Andrews R., Harrison E., Kimberley A., Garnett J.,
RA   Fosker N., Hall R., Garner P., Kelly D., Bird C., Palmer S., Gehring I.,
RA   Berger A., Dooley C.M., Ersan-Urun Z., Eser C., Geiger H., Geisler M.,
RA   Karotki L., Kirn A., Konantz J., Konantz M., Oberlander M.,
RA   Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G., Osoegawa K., Zhu B.,
RA   Rapp A., Widaa S., Langford C., Yang F., Schuster S.C., Carter N.P.,
RA   Harrow J., Ning Z., Herrero J., Searle S.M., Enright A., Geisler R.,
RA   Plasterk R.H., Lee C., Westerfield M., de Jong P.J., Zon L.I.,
RA   Postlethwait J.H., Nusslein-Volhard C., Hubbard T.J., Roest Crollius H.,
RA   Rogers J., Stemple D.L.;
RT   "The zebrafish reference genome sequence and its relationship to the human
RT   genome.";
RL   Nature 496:498-503(2013).
RN   [10] {ECO:0000313|RefSeq:NP_001034904.1}
RP   NUCLEOTIDE SEQUENCE.
RX   PubMed=27802896;
RA   Nathan J.R., Lakshmanan G., Michael F.M., Seppan P., Ragunathan M.;
RT   "Expression of adenosine receptors and vegf during angiogenesis and its
RT   inhibition by pentoxifylline-A study using zebrafish model.";
RL   Biomed. Pharmacother. 84:1406-1418(2016).
RN   [11] {ECO:0000313|RefSeq:NP_001034904.1}
RP   NUCLEOTIDE SEQUENCE.
RX   PubMed=28163255;
RA   Cruz F.F., Leite C.E., Kist L.W., de Oliveira G.M., Bogo M.R., Bonan C.D.,
RA   Campos M.M., Morrone F.B.;
RT   "Effects of caffeine on behavioral and inflammatory changes elicited by
RT   copper in zebrafish larvae: Role of adenosine receptors.";
RL   Comp. Biochem. Physiol. C Toxicol. Pharmacol. 194:28-36(2017).
RN   [12] {ECO:0000313|RefSeq:NP_001034904.1}
RP   NUCLEOTIDE SEQUENCE.
RX   PubMed=28502661;
RA   Wakisaka N., Miyasaka N., Koide T., Masuda M., Hiraki-Kajiyama T.,
RA   Yoshihara Y.;
RT   "An Adenosine Receptor for Olfaction in Fish.";
RL   Curr. Biol. 27:1437-1447(2017).
RN   [13] {ECO:0000313|RefSeq:NP_001034904.1}
RP   NUCLEOTIDE SEQUENCE.
RX   PubMed=29329032;
RA   Panneerselvan N., Ragunathan M.;
RT   "Targeting expression of adenosine receptors during hypoxia induced
RT   angiogenesis - A study using zebrafish model.";
RL   Biomed. Pharmacother. 99:101-112(2018).
RN   [14] {ECO:0000313|RefSeq:NP_001034904.1}
RP   NUCLEOTIDE SEQUENCE.
RX   PubMed=29327202;
RA   Menezes F.P., Machado Torresini F., Nery L.R., da Silva R.S.;
RT   "Transient Disruption of Adenosine Signaling During Embryogenesis Triggers
RT   a Pro-epileptic Phenotype in Adult Zebrafish.";
RL   Mol. Neurobiol. 55:6547-6557(2018).
RN   [15] {ECO:0000313|Ensembl:ENSDARP00000150585}
RP   IDENTIFICATION.
RC   STRAIN=Tuebingen {ECO:0000313|Ensembl:ENSDARP00000150585};
RG   Ensembl;
RL   Submitted (APR-2018) to UniProtKB.
RN   [16] {ECO:0000313|RefSeq:NP_001034904.1, ECO:0000313|RefSeq:XP_005167233.1}
RP   IDENTIFICATION.
RC   STRAIN=Tuebingen {ECO:0000313|RefSeq:XP_005167233.1};
RG   RefSeq;
RL   Submitted (NOV-2023) to UniProtKB.
CC   -!- FUNCTION: Receptor for adenosine. The activity of this receptor is
CC       mediated by G proteins which activate adenylyl cyclase.
CC       {ECO:0000256|RuleBase:RU201114}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000256|ARBA:ARBA00004651,
CC       ECO:0000256|RuleBase:RU201114}; Multi-pass membrane protein
CC       {ECO:0000256|ARBA:ARBA00004651, ECO:0000256|RuleBase:RU201114}.
CC       Membrane {ECO:0000256|ARBA:ARBA00004141}; Multi-pass membrane protein
CC       {ECO:0000256|ARBA:ARBA00004141}.
CC   -!- SIMILARITY: Belongs to the G-protein coupled receptor 1 family.
CC       {ECO:0000256|RuleBase:RU201114}.
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DR   EMBL; AL953914; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; CR547121; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; CR848681; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; BC163368; AAI63368.1; -; mRNA.
DR   EMBL; BC163378; AAI63378.1; -; mRNA.
DR   EMBL; AY945800; AAX63345.1; -; mRNA.
DR   RefSeq; NP_001034904.1; NM_001039815.1.
DR   RefSeq; XP_005167233.1; XM_005167176.4.
DR   STRING; 7955.ENSDARP00000153512; -.
DR   PaxDb; 7955-ENSDARP00000121566; -.
DR   Ensembl; ENSDART00000138750.2; ENSDARP00000121566.1; ENSDARG00000033706.8.
DR   Ensembl; ENSDART00000182752.1; ENSDARP00000150585.1; ENSDARG00000114856.1.
DR   Ensembl; ENSDART00000193749.1; ENSDARP00000153512.1; ENSDARG00000033706.8.
DR   GeneID; 561701; -.
DR   KEGG; dre:561701; -.
DR   AGR; ZFIN:ZDB-GENE-080723-28; -.
DR   CTD; 561701; -.
DR   ZFIN; ZDB-GENE-080723-28; adora2aa.
DR   eggNOG; KOG3656; Eukaryota.
DR   HOGENOM; CLU_009579_11_5_1; -.
DR   OMA; ICICWIL; -.
DR   OrthoDB; 2909020at2759; -.
DR   TreeFam; TF325296; -.
DR   Reactome; R-DRE-417973; Adenosine P1 receptors.
DR   Reactome; R-DRE-418555; G alpha (s) signalling events.
DR   Reactome; R-DRE-5683826; Surfactant metabolism.
DR   Proteomes; UP000000437; Chromosome 8.
DR   Bgee; ENSDARG00000033706; Expressed in heart and 11 other cell types or tissues.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0001609; F:G protein-coupled adenosine receptor activity; IBA:GO_Central.
DR   GO; GO:0007189; P:adenylate cyclase-activating G protein-coupled receptor signaling pathway; IBA:GO_Central.
DR   GO; GO:0001973; P:G protein-coupled adenosine receptor signaling pathway; IBA:GO_Central.
DR   GO; GO:0060047; P:heart contraction; IMP:ZFIN.
DR   GO; GO:0060784; P:regulation of cell proliferation involved in tissue homeostasis; IMP:ZFIN.
DR   GO; GO:0061469; P:regulation of type B pancreatic cell proliferation; IMP:ZFIN.
DR   CDD; cd15068; 7tmA_Adenosine_R_A2A; 1.
DR   Gene3D; 1.20.1070.10; Rhodopsin 7-helix transmembrane proteins; 1.
DR   InterPro; IPR001513; Adeno_A2A_rcpt.
DR   InterPro; IPR001634; Adenosn_rcpt.
DR   InterPro; IPR000276; GPCR_Rhodpsn.
DR   InterPro; IPR017452; GPCR_Rhodpsn_7TM.
DR   PANTHER; PTHR24246:SF47; ADENOSINE RECEPTOR A2A; 1.
DR   PANTHER; PTHR24246; OLFACTORY RECEPTOR AND ADENOSINE RECEPTOR; 1.
DR   Pfam; PF00001; 7tm_1; 1.
DR   PRINTS; PR00553; ADENOSINA2AR.
DR   PRINTS; PR00424; ADENOSINER.
DR   PRINTS; PR00237; GPCRRHODOPSN.
DR   SMART; SM01381; 7TM_GPCR_Srsx; 1.
DR   SUPFAM; SSF81321; Family A G protein-coupled receptor-like; 1.
DR   PROSITE; PS00237; G_PROTEIN_RECEP_F1_1; 1.
DR   PROSITE; PS50262; G_PROTEIN_RECEP_F1_2; 1.
PE   2: Evidence at transcript level;
KW   Cell membrane {ECO:0000256|ARBA:ARBA00022475,
KW   ECO:0000256|RuleBase:RU201114};
KW   Disulfide bond {ECO:0000256|ARBA:ARBA00023157,
KW   ECO:0000256|RuleBase:RU201114};
KW   G-protein coupled receptor {ECO:0000256|ARBA:ARBA00023040,
KW   ECO:0000256|RuleBase:RU201114};
KW   Glycoprotein {ECO:0000256|ARBA:ARBA00023180,
KW   ECO:0000256|RuleBase:RU201114};
KW   Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|RuleBase:RU201114};
KW   Receptor {ECO:0000256|ARBA:ARBA00023170, ECO:0000256|RuleBase:RU201114};
KW   Reference proteome {ECO:0000313|Proteomes:UP000000437};
KW   Transducer {ECO:0000256|ARBA:ARBA00023224, ECO:0000256|RuleBase:RU201114};
KW   Transmembrane {ECO:0000256|ARBA:ARBA00022692,
KW   ECO:0000256|RuleBase:RU201114};
KW   Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW   ECO:0000256|RuleBase:RU201114}.
FT   TRANSMEM        12..32
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|RuleBase:RU201114"
FT   TRANSMEM        44..67
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|RuleBase:RU201114"
FT   TRANSMEM        73..101
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|RuleBase:RU201114"
FT   TRANSMEM        122..144
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|RuleBase:RU201114"
FT   TRANSMEM        176..196
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|RuleBase:RU201114"
FT   TRANSMEM        234..257
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|RuleBase:RU201114"
FT   TRANSMEM        269..289
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|RuleBase:RU201114"
FT   DOMAIN          24..287
FT                   /note="G-protein coupled receptors family 1 profile"
FT                   /evidence="ECO:0000259|PROSITE:PS50262"
FT   REGION          346..372
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   442 AA;  49111 MW;  8603AF4D797FFAEE CRC64;
     MLNNVFDVLY MILELLIALL SVLGNVLVCW AVGLNSNLQS ITNFFVVSLA VADIAVGVLA
     IPFSIVISTG FCANFYGCLF IACFVLVLTQ SSIFSLLAIA IDRYIAIKIP LRYNSLVTGQ
     RARGIIAICW VLSVIIGLTP MLGWHKARLQ EGHNGTCPPG MMECLFEEVV VMDYMVYFNF
     FACVLVPLLL MLAIYLRIFM AARHQLKCIE SKAIPCELKS RSTLQKEVHA AKSLAIIVGL
     FAVCWLPLHI INCFTLFCPE CERPPALIMY LAIILSHANS VVNPFIYAYR IREFRHTFRK
     IVRYHILGRR EPLSCNGSTR TSTRTSVADS LRIKVNGLVR ELYAEQSSTT SSCESAEPGH
     THRPVSTENS ILDNQPIEIS NSHRHTALRH PESPLTGNNE GLACRKHAGL DITDGKDLSS
     PLHIKSALYV QTAHCVELTE VS
//