Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q29RY9

- NPL_BOVIN

UniProt

Q29RY9 - NPL_BOVIN

Protein

N-acetylneuraminate lyase

Gene

NPL

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 67 (01 Oct 2014)
      Sequence version 1 (04 Apr 2006)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalyzes the cleavage of N-acetylneuraminic acid (sialic acid) to form pyruvate and N-acetylmannosamine via a Schiff base intermediate. It prevents sialic acids from being recycled and returning to the cell surface. Involved in the N-glycolylneuraminic acid (Neu5Gc) degradation pathway By similarity.By similarity

    Catalytic activityi

    N-acetylneuraminate = N-acetyl-D-mannosamine + pyruvate.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei143 – 1431Involved in proton transfer during cleavageBy similarity
    Active sitei173 – 1731Schiff-base intermediate with substrateBy similarity

    GO - Molecular functioni

    1. N-acetylneuraminate lyase activity Source: UniProtKB

    GO - Biological processi

    1. carbohydrate metabolic process Source: UniProtKB-KW
    2. N-acetylneuraminate catabolic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Carbohydrate metabolism

    Keywords - Ligandi

    Schiff base

    Enzyme and pathway databases

    ReactomeiREACT_213420. Sialic acid metabolism.
    UniPathwayiUPA00629.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    N-acetylneuraminate lyase (EC:4.1.3.3)
    Short name:
    NALase
    Alternative name(s):
    N-acetylneuraminate pyruvate-lyase
    N-acetylneuraminic acid aldolase
    Sialate lyase
    Sialate-pyruvate lyase
    Sialic acid aldolase
    Sialic acid lyase
    Gene namesi
    Name:NPL
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Chromosome 16

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 320320N-acetylneuraminate lyasePRO_0000273351Add
    BLAST

    Proteomic databases

    PRIDEiQ29RY9.

    Interactioni

    Subunit structurei

    Homotetramer.By similarity

    Protein-protein interaction databases

    STRINGi9913.ENSBTAP00000002925.

    Structurei

    3D structure databases

    ProteinModelPortaliQ29RY9.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni51 – 522Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the DapA family. NanA subfamily.Curated

    Phylogenomic databases

    eggNOGiCOG0329.
    GeneTreeiENSGT00530000063604.
    HOGENOMiHOG000218206.
    HOVERGENiHBG082055.
    InParanoidiQ29RY9.
    KOiK01639.
    OMAiLKPWNKD.
    OrthoDBiEOG7NKKKP.
    TreeFamiTF353639.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    InterProiIPR013785. Aldolase_TIM.
    IPR002220. DapA-like.
    [Graphical view]
    PANTHERiPTHR12128. PTHR12128. 1 hit.
    PfamiPF00701. DHDPS. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001365. DHDPS. 1 hit.
    PRINTSiPR00146. DHPICSNTHASE.

    Sequencei

    Sequence statusi: Complete.

    Q29RY9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MASPKKKLQG LVAATITPMT EHGEINFSVI GRYVDYLVEE QGVKNVFVNG    50
    TTGEGLSLSI SERCQVAEEW VTKGKNKLDQ IVIHVGALSL KESQELAQHA 100
    AEIGADGIAV IAPFFLKPWN KDVLINFLKE VAAAAPALPF YYYHIPALTG 150
    VKIRAEELLD GIQDKIPSFQ GLKFSDTDLL DFGQCVDQNR QRQFAFLFGV 200
    DEQLLSALVM GATGAVGSTY NYLGKKTKQM LEAFERKDFS SALNHQFCIQ 250
    RFINFVVKLG FGVSQTKAIM TLVSGIPMGP PRLPLQKASR EFTDNAEAKL 300
    KSLDFLSCTD LKDGNMEACS 320
    Length:320
    Mass (Da):35,166
    Last modified:April 4, 2006 - v1
    Checksum:iD91F221361E56BA6
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC113332 mRNA. Translation: AAI13333.1.
    RefSeqiNP_001039443.1. NM_001045978.1.
    XP_005217334.1. XM_005217277.1.
    XP_005217335.1. XM_005217278.1.
    UniGeneiBt.26155.

    Genome annotation databases

    EnsembliENSBTAT00000002925; ENSBTAP00000002925; ENSBTAG00000002266.
    GeneIDi507597.
    KEGGibta:507597.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC113332 mRNA. Translation: AAI13333.1 .
    RefSeqi NP_001039443.1. NM_001045978.1.
    XP_005217334.1. XM_005217277.1.
    XP_005217335.1. XM_005217278.1.
    UniGenei Bt.26155.

    3D structure databases

    ProteinModelPortali Q29RY9.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9913.ENSBTAP00000002925.

    Proteomic databases

    PRIDEi Q29RY9.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSBTAT00000002925 ; ENSBTAP00000002925 ; ENSBTAG00000002266 .
    GeneIDi 507597.
    KEGGi bta:507597.

    Organism-specific databases

    CTDi 80896.

    Phylogenomic databases

    eggNOGi COG0329.
    GeneTreei ENSGT00530000063604.
    HOGENOMi HOG000218206.
    HOVERGENi HBG082055.
    InParanoidi Q29RY9.
    KOi K01639.
    OMAi LKPWNKD.
    OrthoDBi EOG7NKKKP.
    TreeFami TF353639.

    Enzyme and pathway databases

    UniPathwayi UPA00629 .
    Reactomei REACT_213420. Sialic acid metabolism.

    Miscellaneous databases

    NextBioi 20868136.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    InterProi IPR013785. Aldolase_TIM.
    IPR002220. DapA-like.
    [Graphical view ]
    PANTHERi PTHR12128. PTHR12128. 1 hit.
    Pfami PF00701. DHDPS. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001365. DHDPS. 1 hit.
    PRINTSi PR00146. DHPICSNTHASE.
    ProtoNeti Search...

    Publicationsi

    1. NIH - Mammalian Gene Collection (MGC) project
      Submitted (FEB-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Hereford.
      Tissue: Uterus.

    Entry informationi

    Entry nameiNPL_BOVIN
    AccessioniPrimary (citable) accession number: Q29RY9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 23, 2007
    Last sequence update: April 4, 2006
    Last modified: October 1, 2014
    This is version 67 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3