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Protein

Myosin-4

Gene

Myh4

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Muscle contraction.Curated

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi179 – 186ATPSequence analysis8

GO - Molecular functioni

GO - Biological processi

  • muscle structure development Source: RGD
  • response to gravity Source: RGD
  • response to muscle activity Source: RGD
  • response to muscle stretch Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Motor protein, Muscle protein, Myosin

Keywords - Ligandi

Actin-binding, ATP-binding, Calmodulin-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Myosin-4
Alternative name(s):
Myosin heavy chain 2b
Short name:
MyHC-2b
Myosin heavy chain 4
Gene namesi
Name:Myh4Imported
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi3139. Myh4.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Thick filament

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002406001 – 1939Myosin-4Add BLAST1939

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei36PhosphoserineCombined sources1
Modified residuei64PhosphothreonineCombined sources1
Modified residuei69PhosphothreonineCombined sources1
Modified residuei79PhosphoserineCombined sources1
Modified residuei130N6,N6,N6-trimethyllysineSequence analysis1
Modified residuei389PhosphotyrosineCombined sources1
Modified residuei391PhosphothreonineCombined sources1
Modified residuei392PhosphoserineCombined sources1
Modified residuei419PhosphothreonineCombined sources1
Modified residuei424PhosphotyrosineCombined sources1
Modified residuei625PhosphoserineCombined sources1
Modified residuei757Pros-methylhistidineBy similarity1
Modified residuei776PhosphothreonineCombined sources1
Modified residuei1092PhosphoserineCombined sources1
Modified residuei1096PhosphoserineCombined sources1
Modified residuei1162PhosphoserineCombined sources1
Modified residuei1237PhosphoserineCombined sources1
Modified residuei1241PhosphothreonineCombined sources1
Modified residuei1243PhosphoserineCombined sources1
Modified residuei1255PhosphothreonineCombined sources1
Modified residuei1261PhosphoserineCombined sources1
Modified residuei1265PhosphothreonineCombined sources1
Modified residuei1278PhosphoserineCombined sources1
Modified residuei1286PhosphothreonineCombined sources1
Modified residuei1288PhosphoserineCombined sources1
Modified residuei1292PhosphoserineCombined sources1
Modified residuei1303PhosphoserineCombined sources1
Modified residuei1306PhosphoserineCombined sources1
Modified residuei1413PhosphoserineCombined sources1
Modified residuei1464PhosphotyrosineCombined sources1
Modified residuei1467PhosphothreonineCombined sources1
Modified residuei1474PhosphoserineCombined sources1
Modified residuei1492PhosphotyrosineCombined sources1
Modified residuei1495PhosphoserineCombined sources1
Modified residuei1501PhosphothreonineCombined sources1
Modified residuei1514PhosphoserineCombined sources1
Modified residuei1517PhosphothreonineCombined sources1
Modified residuei1542PhosphoserineCombined sources1
Modified residuei1547PhosphoserineCombined sources1
Modified residuei1554PhosphoserineCombined sources1
Modified residuei1574PhosphoserineCombined sources1
Modified residuei1600PhosphoserineCombined sources1
Modified residuei1603PhosphoserineCombined sources1
Modified residuei1714PhosphoserineCombined sources1
Modified residuei1726PhosphoserineCombined sources1
Modified residuei1730PhosphothreonineCombined sources1
Modified residuei1736PhosphothreonineCombined sources1
Modified residuei1739PhosphoserineCombined sources1

Keywords - PTMi

Methylation, Phosphoprotein

Proteomic databases

PaxDbiQ29RW1.
PRIDEiQ29RW1.

PTM databases

iPTMnetiQ29RW1.
PhosphoSitePlusiQ29RW1.

Interactioni

Subunit structurei

Muscle myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2).Curated

Protein-protein interaction databases

BioGridi262010. 1 interactor.
IntActiQ29RW1. 1 interactor.
STRINGi10116.ENSRNOP00000046362.

Structurei

3D structure databases

ProteinModelPortaliQ29RW1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini86 – 782Myosin motorAdd BLAST697
Domaini785 – 814IQPROSITE-ProRule annotationAdd BLAST30

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni659 – 681Actin-bindingBy similarityAdd BLAST23
Regioni761 – 775Actin-bindingBy similarityAdd BLAST15

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili843 – 1939Sequence analysisAdd BLAST1097

Domaini

The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.Curated
Limited proteolysis of myosin heavy chain produces 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). HMM can be further cleaved into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2).Curated

Sequence similaritiesi

Contains 1 IQ domain.PROSITE-ProRule annotation
Contains 1 myosin motor domain.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0161. Eukaryota.
COG5022. LUCA.
HOGENOMiHOG000173959.
HOVERGENiHBG004704.
InParanoidiQ29RW1.
KOiK10352.

Family and domain databases

Gene3Di4.10.270.10. 1 hit.
InterProiIPR000048. IQ_motif_EF-hand-BS.
IPR027401. Myosin-like_IQ_dom.
IPR001609. Myosin_head_motor_dom.
IPR004009. Myosin_N.
IPR002928. Myosin_tail.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00063. Myosin_head. 1 hit.
PF02736. Myosin_N. 1 hit.
PF01576. Myosin_tail_1. 1 hit.
[Graphical view]
PRINTSiPR00193. MYOSINHEAVY.
SMARTiSM00242. MYSc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS50096. IQ. 1 hit.
PS51456. MYOSIN_MOTOR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q29RW1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSDAEMAVF GEAAPYLRKS EKERIEAQNK PFDAKSSVFV VDAKESYVKA
60 70 80 90 100
TVQSREGGKV TAKTEGGATV TVKEDQVFSM NPPKYDKIED MAMMTHLHEP
110 120 130 140 150
AVLYNLKERY AAWMIYTYSG LFCVTVNPYK WLPVYNPEVV AAYRGKKRQE
160 170 180 190 200
APPHIFSISD NAYQFMLTDR ENQSILITGE SGAGKTVNTK RVIQYFATIA
210 220 230 240 250
VTGDKKKEEA PSGKMQGTLE DQIISANPLL EAFGNAKTVR NDNSSRFGKF
260 270 280 290 300
IRIHFGATGK LASADIETYL LEKSRVTFQL KAERSYHIFY QVMSNKKPEL
310 320 330 340 350
IEMLLITTNP YDFAYVSQGE ITVPSIDDQE ELMATDTAVD ILGFTADEKV
360 370 380 390 400
AIYKLTGAVM HYGNMKFKQK QREEQAEPDG TEVADKAAYL TSLNSADLLK
410 420 430 440 450
ALCYPRVKVG NEYVTKGQTV QQVYNSVGAL AKAMYEKMFL WMVTRINQQL
460 470 480 490 500
DTKQPRQYFI GVLDIAGFEI FDFNTLEQLC INFTNEKLQQ FFNHHMFVLE
510 520 530 540 550
QEEYKKEGIE WEFIDFGMDL AACIELIEKP MGIFSILEEE CMFPKATDTS
560 570 580 590 600
FKNKLYEQHL GKSNNFQKPK PAKGKAEAHF SLVHYAGTVD YNIIGWLDKN
610 620 630 640 650
KDPLNETVVG LYQKSGLKTL AFLFSGGQAA EAEGGGGKKG GKKKGSSFQT
660 670 680 690 700
VSALFRENLN KLMTNLKSTH PHFVRCLIPN ETKTPGAMEH ELVLHQLRCN
710 720 730 740 750
GVLEGIRICR KGFPSRILYA DFKQRYKVLN ASAIPEGQFI DSKKASEKLL
760 770 780 790 800
GSIDIDHTQY KFGHTKVFFK AGLLGTLEEM RDEKLAQLIT RTQAVCRGYL
810 820 830 840 850
MRVEFRKMME RRESIFCIQY NVRAFMNVKH WPWMKLYFKI KPLLKSAETE
860 870 880 890 900
KEMATMKEDF EKAKEDLAKS EAKRKELEEK MVALMQEKND LQLQVQAEAD
910 920 930 940 950
GLADAEERCD QLIKTKIQLE AKIKELTERA EDEEEINAEL TAKKRKLEDE
960 970 980 990 1000
CSELKKDIDD LELTLAKVEK EKHATENKVK NLTEEMAGLD ENIVKLTKEK
1010 1020 1030 1040 1050
KALQEAHQQT LDDLQAEEDK VNTLTKAKTK LEQQVDDLEG SLEQEKKLRM
1060 1070 1080 1090 1100
DLERAKRKLE GDLKLAQEST MDIENDKQQL DEKLKKKEFE MSNLQSKIED
1110 1120 1130 1140 1150
EQALGMQLQK KIKELQARIE ELEEEIEAER ASRAKAEKQR SDLSRELEEI
1160 1170 1180 1190 1200
SERLEEAGGA TSAQIEMNKK REAEFQKMRR DLEEATLQHE ATAAALRKKH
1210 1220 1230 1240 1250
ADSVAELGEQ IDNLQRVKQK LEKEKSELKM EIDDLASNME TVSKAKGNLE
1260 1270 1280 1290 1300
KMCRTLEDQL SEVKTKEEEQ QRLINELSAQ KARLHTESGE FSRQLDEKDA
1310 1320 1330 1340 1350
MVSQLSRGKQ AFTQQIEELK RQLEEESKAK NALAHALQSA RHDCDLLREQ
1360 1370 1380 1390 1400
YEEEQEAKAE LQRAMSKANS EVAQWRTKYE TDAIQRTEEL EEAKKKLAQR
1410 1420 1430 1440 1450
LQDAEEHVEA VNSKCASLEK TKQRLQNEVE DLMIDVERSN AACAALDKKQ
1460 1470 1480 1490 1500
RNFDKVLAEW KQKYEETQAE LEASQKESRS LSTELFKVKN AYEESLDQLE
1510 1520 1530 1540 1550
TLKRENKNLQ QEISDLTEQI AEGGKHIHEL EKIKKQIDQE KSELQASLEE
1560 1570 1580 1590 1600
AEASLEHEEG KILRIQLELN QVKSEIDRKI AEKDEEIDQL KRNHLRVVES
1610 1620 1630 1640 1650
MQSTLDAEIR SRNDALRIKK KMEGDLNEME IQLNHANRQA AEAIRNLRNT
1660 1670 1680 1690 1700
QGMLKDTQLH LDDALRGQDD LKEQLAMVER RANLMQAEIE ELRASLEQTE
1710 1720 1730 1740 1750
RSRRVAEQEL LDASERVQLL HTQNTSLINT KKKLETDISQ IQGEMEDIVQ
1760 1770 1780 1790 1800
EARNAEEKAK KAITDAAMMA EELKKEQDTS AHLERMKKNM EQTVKDLQHR
1810 1820 1830 1840 1850
LDEAEQLALK GGKKQIQKLE ARVRELENEV ENEQKRNIEA VKGLRKHERR
1860 1870 1880 1890 1900
VKELTYQTEE DRKNVLRLQD LVDKLQTKVK AYKRQAEEAE EQSNVNLAKF
1910 1920 1930
RKIQHELEEA EERADIAESQ VNKLRVKSRE VHTKVISEE
Length:1,939
Mass (Da):222,880
Last modified:April 4, 2006 - v1
Checksum:iD875C3B566DE8151
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC113948 mRNA. Translation: AAI13949.1.
RefSeqiNP_062198.1. NM_019325.1.
UniGeneiRn.224464.

Genome annotation databases

GeneIDi360543.
KEGGirno:360543.
UCSCiRGD:3139. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC113948 mRNA. Translation: AAI13949.1.
RefSeqiNP_062198.1. NM_019325.1.
UniGeneiRn.224464.

3D structure databases

ProteinModelPortaliQ29RW1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi262010. 1 interactor.
IntActiQ29RW1. 1 interactor.
STRINGi10116.ENSRNOP00000046362.

PTM databases

iPTMnetiQ29RW1.
PhosphoSitePlusiQ29RW1.

Proteomic databases

PaxDbiQ29RW1.
PRIDEiQ29RW1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi360543.
KEGGirno:360543.
UCSCiRGD:3139. rat.

Organism-specific databases

CTDi4622.
RGDi3139. Myh4.

Phylogenomic databases

eggNOGiKOG0161. Eukaryota.
COG5022. LUCA.
HOGENOMiHOG000173959.
HOVERGENiHBG004704.
InParanoidiQ29RW1.
KOiK10352.

Miscellaneous databases

PROiQ29RW1.

Family and domain databases

Gene3Di4.10.270.10. 1 hit.
InterProiIPR000048. IQ_motif_EF-hand-BS.
IPR027401. Myosin-like_IQ_dom.
IPR001609. Myosin_head_motor_dom.
IPR004009. Myosin_N.
IPR002928. Myosin_tail.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00063. Myosin_head. 1 hit.
PF02736. Myosin_N. 1 hit.
PF01576. Myosin_tail_1. 1 hit.
[Graphical view]
PRINTSiPR00193. MYOSINHEAVY.
SMARTiSM00242. MYSc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS50096. IQ. 1 hit.
PS51456. MYOSIN_MOTOR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMYH4_RAT
AccessioniPrimary (citable) accession number: Q29RW1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: April 4, 2006
Last modified: November 30, 2016
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

Represents a conventional myosin. This protein should not be confused with the unconventional myosin-4 (MYO4).Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.