Reviewed, UniProtKB/Swiss-Prot Q29RV1 (PDIA4_BOVIN)

Names and origin

Protein names

Recommended name:
    Protein disulfide-isomerase A4
    EC=5.3.4.1

Gene names

Name:

PDIA4

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	643 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Catalyzes the rearrangement of -S-S- bonds in proteins.
Subunit structure	Part a large chaperone multiprotein complex comprising CABP1, DNAJB11, HSP90B1, HSPA5, HYOU, PDIA2, PDIA4, PPIB, SDF2L1, UGT1A1 and very small amounts of ERP29, but not, or at very low levels, CALR nor CANX.
Subcellular location	Endoplasmic reticulum lumen By similarity. Melanosome By similarity.
Sequence similarities	Belongs to the protein disulfide isomerase family. Contains 3 thioredoxin domains.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum
Domain	Redox-active center Repeat Signal
Molecular function	Isomerase
PTM	Disulfide bond
Gene Ontology (GO)
Biological process	cell redox homeostasis Inferred from electronic annotation. Source: InterPro
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW endoplasmic reticulum lumen Inferred from electronic annotation. Source: UniProtKB-SubCell melanosome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein binding Inferred from electronic annotation. Source: InterPro protein disulfide isomerase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	Potential
	Chain	21 – 643	623	Protein disulfide-isomerase A4		PRO_0000240338
Regions
	Domain	21 – 168	148	Thioredoxin 1
	Domain	170 – 300	131	Thioredoxin 2
	Domain	503 – 634	132	Thioredoxin 3
	Motif	640 – 643	4	Prevents secretion from ER By similarity
	Compositional bias	39 – 54	16	Asp/Glu-rich (acidic)
Amino acid modifications
	Disulfide bond	90 ↔ 93		Redox-active By similarity
	Disulfide bond	205 ↔ 208		Redox-active By similarity
	Disulfide bond	553 ↔ 556		Redox-active By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q29RV1-1 [UniParc]. Last modified April 4, 2006. Version 1. Checksum: EFBBB755DE97A35E	FASTA	643	72,526
	10 20 30 40 50 60 MRPRKAWMLV LLLALVQLLA VASAGAPDED STDKEDAIEE DEEEDEDDDD DDDDLEVKEE 70 80 90 100 110 120 NGVLILNDAN FDNFVADKDT VLLEFYAPWC GHCKQFAPEY EKIAATLKEN DPPIPVAKID 130 140 150 160 170 180 ATSESALASR FDVSGYPTIK ILKKGQEVDY EGSRTQEEIV AKVKEVSQPN WTPPPEVTLV 190 200 210 220 230 240 LTKDNFDEVV NDADIILVEF YAPWCGHCKK LAPEYEKAAK ELSKSSPPIP LAKVDAIAET 250 260 270 280 290 300 DLAKRFDVSS YPTLKIFRKG KAFSYNGPRE KYGIVDYMME QSGPPSKQIL ALKQVQEFLK 310 320 330 340 350 360 DGDDVIIIGV FKSESDPAYQ LYQDAANSLR EDYKFHHTFS TEIAKFLKVS LGKLVVMQPE 370 380 390 400 410 420 KFQSKYEPKS YVMDIKDSTE AAAITEHVVK HTLPLVGHRK AADAKRYTRR PLVVVYYSVD 430 440 450 460 470 480 FSFDYRAATQ FWRNKVLEVA KDFPEYTFAV ADEEDFATEL KDLGLSESGE EVNAAILDEG 490 500 510 520 530 540 GRRFAMEPDD FDADALRDFV TAFKKGKLKP VIKSQPVPKN NKGPVKVVVG KTFDSIVMDP 550 560 570 580 590 600 KKDVLIEFYA PWCGHCKQLE PVYTSLGKKY KGHKNLVIAK MDATANDVTS DRYKVEGFPT 610 620 630 640 IYFAPSGDKK KPIKFEDGNR DLEHLSKFIE EHATKLSRTK EEL

References

[1]	NIH - Mammalian Gene Collection (MGC) project Submitted (FEB-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Testis.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC114004 mRNA. Translation: AAI14005.1.
IPI	IPI00706136.
RefSeq	NP_001039344.1.
UniGene	Bt.23128
3D structure databases
SMR	Q29RV1. Positions 55-178, 177-283, 521-643.
ModBase	Search...
Genome annotation databases
Ensembl	ENSBTAG00000017143. Bos taurus. [Contig view]
GeneID	415110.
KEGG	bta:415110.
Phylogenomic databases
HOVERGEN	Q29RV1.
Enzyme and pathway databases
BRENDA	5.3.4.1. 251.
Family and domain databases
InterPro	IPR005788. Disulphide_isomerase. IPR000886. ER_targeting_sequence. IPR005792. Prot_disulphide_isomerase. IPR017068. Protein_diS-isomerase_A4. IPR017936. Thioredoxin-like. IPR006662. Thioredoxin-like_subdom. IPR017937. Thioredoxin_CS. IPR013766. Thioredoxin_domain. IPR012335. Thioredoxin_fold. [Graphical view]
Gene3D	G3DSA:3.40.30.10. Thioredoxin_fold. 3 hits.
Pfam	PF00085. Thioredoxin. 3 hits. [Graphical view]
PIRSF	PIRSF036862. Disulphide_isom_A4. 1 hit.
PRINTS	PR00421. THIOREDOXIN.
TIGRFAMs	TIGR01130. ER_PDI_fam. 1 hit. TIGR01126. pdi_dom. 3 hits.
PROSITE	PS00014. ER_TARGET. 1 hit. PS00194. THIOREDOXIN_1. 3 hits. PS51352. THIOREDOXIN_2. 3 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PDIA4_BOVIN

Accession

Primary (citable) accession number: Q29RV1

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 27, 2006
Last sequence update:	April 4, 2006
Last modified:	June 16, 2009
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)