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Protein

Ubiquitin carboxyl-terminal hydrolase 1

Gene

USP1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Negative regulator of DNA damage repair which specifically deubiquitinates monoubiquitinated FANCD2. Also involved in PCNA-mediated translesion synthesis (TLS) by deubiquitinating monoubiquitinated PCNA. Has almost no deubiquitinating activity by itself and requires the interaction with WDR48 to have a high activity (By similarity).By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei90 – 901NucleophilePROSITE-ProRule annotation
Active sitei591 – 5911Proton acceptorPROSITE-ProRule annotation
Sitei669 – 6702Cleavage; by autolysisBy similarity

GO - Molecular functioni

  1. ubiquitin-specific protease activity Source: UniProtKB

GO - Biological processi

  1. DNA repair Source: UniProtKB-KW
  2. monoubiquitinated protein deubiquitination Source: UniProtKB
  3. protein deubiquitination Source: UniProtKB
  4. regulation of DNA repair Source: UniProtKB
  5. response to UV Source: UniProtKB
  6. ubiquitin-dependent protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

DNA damage, DNA repair, Ubl conjugation pathway

Protein family/group databases

MEROPSiC19.019.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 1 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 1
Ubiquitin thioesterase 1
Ubiquitin-specific-processing protease 1
Gene namesi
Name:USP1By similarity
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136 Componenti: Unplaced

Subcellular locationi

Nucleus By similarity

GO - Cellular componenti

  1. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 783783Ubiquitin carboxyl-terminal hydrolase 1PRO_0000306286Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei42 – 421PhosphoserineBy similarity
Modified residuei67 – 671PhosphoserineBy similarity
Modified residuei473 – 4731PhosphoserineBy similarity

Post-translational modificationi

Autocatalytic cleavage of USP1 following UV irradiation inactivates it leading to an increase in ubiquitinated PCNA, recruitment of POLH and translesion synthesis.By similarity
Ubiquitinated; leading to its subsequent proteasomal degradation.By similarity

Keywords - PTMi

Autocatalytic cleavage, Phosphoprotein, Ubl conjugation

Proteomic databases

PRIDEiQ29RP1.

Interactioni

Subunit structurei

Interacts with FANCD2 and PCNA. Interacts with WDR48 (By similarity).By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000027254.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini81 – 783703USPAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase C19 family.Sequence Analysis
Contains 1 USP domain.Curated

Phylogenomic databases

eggNOGiCOG5077.
HOGENOMiHOG000154756.
HOVERGENiHBG017288.
InParanoidiQ29RP1.
KOiK11832.

Family and domain databases

InterProiIPR001394. Peptidase_C19_UCH.
IPR018200. USP_CS.
IPR028889. USP_dom.
[Graphical view]
PfamiPF00443. UCH. 2 hits.
[Graphical view]
PROSITEiPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q29RP1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPGVIPSESN GLSRGSPSKK NRLSLKFFQK KETKRALDFT DSQENEEKTS
60 70 80 90 100
EYKGSEIDQV VPAAQSSPIN CEKRENLLPF VGLNNLGNTC YLNSILQVLY
110 120 130 140 150
FCPGFKSGVK HLFNIISRKK EALKDEANQK DKGNCKEDSL ASYELICSLQ
160 170 180 190 200
SLIISVEQLQ ASFLLNPEKY TDELATQPRR LLNTLRELNP MYEGYLQHDA
210 220 230 240 250
QEVLQCILGN IQETCQLLKK EEVKNVEDLS TKVEEYQKEE MSDNNSMEMD
260 270 280 290 300
NMRHSEDYKE KLSKGNGKRK SDAEFGNMKK KVKISKEHQS SEENQRQTRS
310 320 330 340 350
KRKAAGDTLE ISHKIIPKHI SENESTRPSQ RKSKVKINWL KSAAKQPSIL
360 370 380 390 400
SKFCSMGKIA TNQGSKGHCK ENEYDLEEDL GKYENDNTTN DCELESPGNN
410 420 430 440 450
DMPVHVNEVK PINKGAEQIG FELVEKLFQG QLVLRTRCLE CESLTERRED
460 470 480 490 500
FQDISVPVQE DELSKVEENS EISPEPKTEM KTLRWAISQF ASVERIVGED
510 520 530 540 550
KYFCENCHHY TEAERSLLFD KMPEVITIHL KCFAASGLEF DCYGGGLSKI
560 570 580 590 600
NTPLLTPLKL SLEEWSTKPT NDSYGLFAVV MHSGITISSG HYTASVKVTD
610 620 630 640 650
LNSLELDKEN FVIDQTCEIG KPEPLNEEEV RGVVENYDNE EVSIRVSGNN
660 670 680 690 700
QPSKVLNKKN VEAIGLLGGQ KSKADYELYN KASNPDKVAS TALPENRNSE
710 720 730 740 750
TNNTNGTDES DSNKESSDQT GINISGFENK ISYVVQSLKE YEGKWLLFDD
760 770 780
SEVKVTEEKD FLNSLSPSTS PTSTPYLLFY KKL
Length:783
Mass (Da):88,459
Last modified:April 3, 2006 - v1
Checksum:i7910D2FC5668E87C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC114087 mRNA. Translation: AAI14088.1.
RefSeqiNP_001039689.1. NM_001046224.1.
UniGeneiBt.40485.

Genome annotation databases

GeneIDi516328.
KEGGibta:516328.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC114087 mRNA. Translation: AAI14088.1.
RefSeqiNP_001039689.1. NM_001046224.1.
UniGeneiBt.40485.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000027254.

Protein family/group databases

MEROPSiC19.019.

Proteomic databases

PRIDEiQ29RP1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi516328.
KEGGibta:516328.

Organism-specific databases

CTDi7398.

Phylogenomic databases

eggNOGiCOG5077.
HOGENOMiHOG000154756.
HOVERGENiHBG017288.
InParanoidiQ29RP1.
KOiK11832.

Miscellaneous databases

NextBioi20872192.

Family and domain databases

InterProiIPR001394. Peptidase_C19_UCH.
IPR018200. USP_CS.
IPR028889. USP_dom.
[Graphical view]
PfamiPF00443. UCH. 2 hits.
[Graphical view]
PROSITEiPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. NIH - Mammalian Gene Collection (MGC) project
    Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: HerefordImported.
    Tissue: Heart ventricleImported.

Entry informationi

Entry nameiUBP1_BOVIN
AccessioniPrimary (citable) accession number: Q29RP1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 2007
Last sequence update: April 3, 2006
Last modified: March 31, 2015
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.