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Q29RP1

- UBP1_BOVIN

UniProt

Q29RP1 - UBP1_BOVIN

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Protein

Ubiquitin carboxyl-terminal hydrolase 1

Gene
USP1
Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Negative regulator of DNA damage repair which specifically deubiquitinates monoubiquitinated FANCD2. Also involved in PCNA-mediated translesion synthesis (TLS) by deubiquitinating monoubiquitinated PCNA. Has almost no deubiquitinating activity by itself and requires the interaction with WDR48 to have a high activity By similarity.

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei90 – 901Nucleophile By similarityBy similarity
Active sitei591 – 5911Proton acceptor By similarityBy similarity
Sitei669 – 6702Cleavage; by autolysis By similarityBy similarity

GO - Molecular functioni

  1. ubiquitin-specific protease activity Source: UniProtKB
  2. ubiquitin thiolesterase activity Source: UniProtKB

GO - Biological processi

  1. DNA repair Source: UniProtKB-KW
  2. monoubiquitinated protein deubiquitination Source: UniProtKB
  3. protein deubiquitination Source: UniProtKB
  4. regulation of DNA repair Source: UniProtKB
  5. response to UV Source: UniProtKB
  6. ubiquitin-dependent protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

DNA damage, DNA repair, Ubl conjugation pathway

Protein family/group databases

MEROPSiC19.019.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 1 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 1
Ubiquitin thioesterase 1
Ubiquitin-specific-processing protease 1
Gene namesi
Name:USP1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Unplaced

Subcellular locationi

Nucleus By similarity By similarity

GO - Cellular componenti

  1. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 783783Ubiquitin carboxyl-terminal hydrolase 1PRO_0000306286Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei42 – 421Phosphoserine By similarityBy similarity
Modified residuei67 – 671Phosphoserine By similarity
Modified residuei473 – 4731Phosphoserine By similarity

Post-translational modificationi

Autocatalytic cleavage of USP1 following UV irradiation inactivates it leading to an increase in ubiquitinated PCNA, recruitment of POLH and translesion synthesis By similarity.
Ubiquitinated; leading to its subsequent proteasomal degradation By similarity.

Keywords - PTMi

Autocatalytic cleavage, Phosphoprotein, Ubl conjugation

Proteomic databases

PRIDEiQ29RP1.

Interactioni

Subunit structurei

Interacts with FANCD2 and PCNA. Interacts with WDR48 By similarity.

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000027254.

Structurei

3D structure databases

ProteinModelPortaliQ29RP1.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini81 – 783703USPAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase C19 family.
Contains 1 USP domain.

Phylogenomic databases

eggNOGiCOG5077.
HOGENOMiHOG000154756.
HOVERGENiHBG017288.
InParanoidiQ29RP1.
KOiK11832.

Family and domain databases

InterProiIPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
[Graphical view]
PfamiPF00443. UCH. 2 hits.
[Graphical view]
PROSITEiPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q29RP1-1 [UniParc]FASTAAdd to Basket

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MPGVIPSESN GLSRGSPSKK NRLSLKFFQK KETKRALDFT DSQENEEKTS    50
EYKGSEIDQV VPAAQSSPIN CEKRENLLPF VGLNNLGNTC YLNSILQVLY 100
FCPGFKSGVK HLFNIISRKK EALKDEANQK DKGNCKEDSL ASYELICSLQ 150
SLIISVEQLQ ASFLLNPEKY TDELATQPRR LLNTLRELNP MYEGYLQHDA 200
QEVLQCILGN IQETCQLLKK EEVKNVEDLS TKVEEYQKEE MSDNNSMEMD 250
NMRHSEDYKE KLSKGNGKRK SDAEFGNMKK KVKISKEHQS SEENQRQTRS 300
KRKAAGDTLE ISHKIIPKHI SENESTRPSQ RKSKVKINWL KSAAKQPSIL 350
SKFCSMGKIA TNQGSKGHCK ENEYDLEEDL GKYENDNTTN DCELESPGNN 400
DMPVHVNEVK PINKGAEQIG FELVEKLFQG QLVLRTRCLE CESLTERRED 450
FQDISVPVQE DELSKVEENS EISPEPKTEM KTLRWAISQF ASVERIVGED 500
KYFCENCHHY TEAERSLLFD KMPEVITIHL KCFAASGLEF DCYGGGLSKI 550
NTPLLTPLKL SLEEWSTKPT NDSYGLFAVV MHSGITISSG HYTASVKVTD 600
LNSLELDKEN FVIDQTCEIG KPEPLNEEEV RGVVENYDNE EVSIRVSGNN 650
QPSKVLNKKN VEAIGLLGGQ KSKADYELYN KASNPDKVAS TALPENRNSE 700
TNNTNGTDES DSNKESSDQT GINISGFENK ISYVVQSLKE YEGKWLLFDD 750
SEVKVTEEKD FLNSLSPSTS PTSTPYLLFY KKL 783
Length:783
Mass (Da):88,459
Last modified:April 4, 2006 - v1
Checksum:i7910D2FC5668E87C
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC114087 mRNA. Translation: AAI14088.1.
RefSeqiNP_001039689.1. NM_001046224.1.
UniGeneiBt.40485.

Genome annotation databases

GeneIDi516328.
KEGGibta:516328.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC114087 mRNA. Translation: AAI14088.1 .
RefSeqi NP_001039689.1. NM_001046224.1.
UniGenei Bt.40485.

3D structure databases

ProteinModelPortali Q29RP1.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9913.ENSBTAP00000027254.

Protein family/group databases

MEROPSi C19.019.

Proteomic databases

PRIDEi Q29RP1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 516328.
KEGGi bta:516328.

Organism-specific databases

CTDi 7398.

Phylogenomic databases

eggNOGi COG5077.
HOGENOMi HOG000154756.
HOVERGENi HBG017288.
InParanoidi Q29RP1.
KOi K11832.

Miscellaneous databases

NextBioi 20872192.

Family and domain databases

InterProi IPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
[Graphical view ]
Pfami PF00443. UCH. 2 hits.
[Graphical view ]
PROSITEi PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. NIH - Mammalian Gene Collection (MGC) project
    Submitted (FEB-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Heart ventricle.

Entry informationi

Entry nameiUBP1_BOVIN
AccessioniPrimary (citable) accession number: Q29RP1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: April 4, 2006
Last modified: April 16, 2014
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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