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Q29RP1

- UBP1_BOVIN

UniProt

Q29RP1 - UBP1_BOVIN

Protein

Ubiquitin carboxyl-terminal hydrolase 1

Gene

USP1

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 57 (01 Oct 2014)
      Sequence version 1 (04 Apr 2006)
      Previous versions | rss
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    Functioni

    Negative regulator of DNA damage repair which specifically deubiquitinates monoubiquitinated FANCD2. Also involved in PCNA-mediated translesion synthesis (TLS) by deubiquitinating monoubiquitinated PCNA. Has almost no deubiquitinating activity by itself and requires the interaction with WDR48 to have a high activity By similarity.By similarity

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei90 – 901NucleophilePROSITE-ProRule annotation
    Active sitei591 – 5911Proton acceptorPROSITE-ProRule annotation
    Sitei669 – 6702Cleavage; by autolysisBy similarity

    GO - Molecular functioni

    1. ubiquitin-specific protease activity Source: UniProtKB
    2. ubiquitin thiolesterase activity Source: UniProtKB

    GO - Biological processi

    1. DNA repair Source: UniProtKB-KW
    2. monoubiquitinated protein deubiquitination Source: UniProtKB
    3. protein deubiquitination Source: UniProtKB
    4. regulation of DNA repair Source: UniProtKB
    5. response to UV Source: UniProtKB
    6. ubiquitin-dependent protein catabolic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    DNA damage, DNA repair, Ubl conjugation pathway

    Protein family/group databases

    MEROPSiC19.019.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin carboxyl-terminal hydrolase 1 (EC:3.4.19.12)
    Alternative name(s):
    Deubiquitinating enzyme 1
    Ubiquitin thioesterase 1
    Ubiquitin-specific-processing protease 1
    Gene namesi
    Name:USP1By similarity
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Unplaced

    Subcellular locationi

    Nucleus By similarity

    GO - Cellular componenti

    1. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 783783Ubiquitin carboxyl-terminal hydrolase 1PRO_0000306286Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei42 – 421PhosphoserineBy similarity
    Modified residuei67 – 671PhosphoserineBy similarity
    Modified residuei473 – 4731PhosphoserineBy similarity

    Post-translational modificationi

    Autocatalytic cleavage of USP1 following UV irradiation inactivates it leading to an increase in ubiquitinated PCNA, recruitment of POLH and translesion synthesis.By similarity
    Ubiquitinated; leading to its subsequent proteasomal degradation.By similarity

    Keywords - PTMi

    Autocatalytic cleavage, Phosphoprotein, Ubl conjugation

    Proteomic databases

    PRIDEiQ29RP1.

    Interactioni

    Subunit structurei

    Interacts with FANCD2 and PCNA. Interacts with WDR48 By similarity.By similarity

    Protein-protein interaction databases

    STRINGi9913.ENSBTAP00000027254.

    Structurei

    3D structure databases

    ProteinModelPortaliQ29RP1.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini81 – 783703USPAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase C19 family.Sequence Analysis
    Contains 1 USP domain.Curated

    Phylogenomic databases

    eggNOGiCOG5077.
    HOGENOMiHOG000154756.
    HOVERGENiHBG017288.
    InParanoidiQ29RP1.
    KOiK11832.

    Family and domain databases

    InterProiIPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR028889. UCH/PAN2.
    [Graphical view]
    PfamiPF00443. UCH. 2 hits.
    [Graphical view]
    PROSITEiPS00972. USP_1. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q29RP1-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPGVIPSESN GLSRGSPSKK NRLSLKFFQK KETKRALDFT DSQENEEKTS    50
    EYKGSEIDQV VPAAQSSPIN CEKRENLLPF VGLNNLGNTC YLNSILQVLY 100
    FCPGFKSGVK HLFNIISRKK EALKDEANQK DKGNCKEDSL ASYELICSLQ 150
    SLIISVEQLQ ASFLLNPEKY TDELATQPRR LLNTLRELNP MYEGYLQHDA 200
    QEVLQCILGN IQETCQLLKK EEVKNVEDLS TKVEEYQKEE MSDNNSMEMD 250
    NMRHSEDYKE KLSKGNGKRK SDAEFGNMKK KVKISKEHQS SEENQRQTRS 300
    KRKAAGDTLE ISHKIIPKHI SENESTRPSQ RKSKVKINWL KSAAKQPSIL 350
    SKFCSMGKIA TNQGSKGHCK ENEYDLEEDL GKYENDNTTN DCELESPGNN 400
    DMPVHVNEVK PINKGAEQIG FELVEKLFQG QLVLRTRCLE CESLTERRED 450
    FQDISVPVQE DELSKVEENS EISPEPKTEM KTLRWAISQF ASVERIVGED 500
    KYFCENCHHY TEAERSLLFD KMPEVITIHL KCFAASGLEF DCYGGGLSKI 550
    NTPLLTPLKL SLEEWSTKPT NDSYGLFAVV MHSGITISSG HYTASVKVTD 600
    LNSLELDKEN FVIDQTCEIG KPEPLNEEEV RGVVENYDNE EVSIRVSGNN 650
    QPSKVLNKKN VEAIGLLGGQ KSKADYELYN KASNPDKVAS TALPENRNSE 700
    TNNTNGTDES DSNKESSDQT GINISGFENK ISYVVQSLKE YEGKWLLFDD 750
    SEVKVTEEKD FLNSLSPSTS PTSTPYLLFY KKL 783
    Length:783
    Mass (Da):88,459
    Last modified:April 4, 2006 - v1
    Checksum:i7910D2FC5668E87C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC114087 mRNA. Translation: AAI14088.1.
    RefSeqiNP_001039689.1. NM_001046224.1.
    UniGeneiBt.40485.

    Genome annotation databases

    GeneIDi516328.
    KEGGibta:516328.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC114087 mRNA. Translation: AAI14088.1 .
    RefSeqi NP_001039689.1. NM_001046224.1.
    UniGenei Bt.40485.

    3D structure databases

    ProteinModelPortali Q29RP1.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9913.ENSBTAP00000027254.

    Protein family/group databases

    MEROPSi C19.019.

    Proteomic databases

    PRIDEi Q29RP1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 516328.
    KEGGi bta:516328.

    Organism-specific databases

    CTDi 7398.

    Phylogenomic databases

    eggNOGi COG5077.
    HOGENOMi HOG000154756.
    HOVERGENi HBG017288.
    InParanoidi Q29RP1.
    KOi K11832.

    Miscellaneous databases

    NextBioi 20872192.

    Family and domain databases

    InterProi IPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR028889. UCH/PAN2.
    [Graphical view ]
    Pfami PF00443. UCH. 2 hits.
    [Graphical view ]
    PROSITEi PS00972. USP_1. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. NIH - Mammalian Gene Collection (MGC) project
      Submitted (FEB-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: HerefordImported.
      Tissue: Heart ventricleImported.

    Entry informationi

    Entry nameiUBP1_BOVIN
    AccessioniPrimary (citable) accession number: Q29RP1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 2, 2007
    Last sequence update: April 4, 2006
    Last modified: October 1, 2014
    This is version 57 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3