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Protein

Ubiquitin carboxyl-terminal hydrolase 1

Gene

USP1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Negative regulator of DNA damage repair which specifically deubiquitinates monoubiquitinated FANCD2. Also involved in PCNA-mediated translesion synthesis (TLS) by deubiquitinating monoubiquitinated PCNA. Has almost no deubiquitinating activity by itself and requires the interaction with WDR48 to have a high activity.By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei90NucleophilePROSITE-ProRule annotation1
Active sitei591Proton acceptorPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

DNA damage, DNA repair, Ubl conjugation pathway

Protein family/group databases

MEROPSiC19.019.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 1 (EC:3.4.19.12By similarity)
Alternative name(s):
Deubiquitinating enzyme 1
Ubiquitin thioesterase 1
Ubiquitin-specific-processing protease 1
Gene namesi
Name:USP1By similarity
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

  • Nucleus By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003062861 – 783Ubiquitin carboxyl-terminal hydrolase 1Add BLAST783

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei16PhosphoserineBy similarity1
Modified residuei42PhosphoserineBy similarity1
Modified residuei67PhosphoserineBy similarity1
Modified residuei473PhosphoserineBy similarity1
Modified residuei766PhosphoserineBy similarity1

Post-translational modificationi

Autocatalytic cleavage of USP1 following UV irradiation inactivates it leading to an increase in ubiquitinated PCNA, recruitment of POLH and translesion synthesis.By similarity
Ubiquitinated; leading to its subsequent proteasomal degradation.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei669 – 670Cleavage; by autolysisBy similarity2

Keywords - PTMi

Autocatalytic cleavage, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ29RP1.
PRIDEiQ29RP1.

Interactioni

Subunit structurei

Interacts with FANCD2 and PCNA. Interacts with WDR48.By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000027254.

Structurei

3D structure databases

ProteinModelPortaliQ29RP1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini81 – 783USPAdd BLAST703

Sequence similaritiesi

Belongs to the peptidase C19 family.Sequence analysis
Contains 1 USP domain.Curated

Phylogenomic databases

eggNOGiKOG1864. Eukaryota.
ENOG410XQ81. LUCA.
HOGENOMiHOG000154756.
HOVERGENiHBG017288.
InParanoidiQ29RP1.
KOiK11832.

Family and domain databases

CDDicd02671. Peptidase_C19O. 1 hit.
InterProiIPR001394. Peptidase_C19_UCH.
IPR033815. USP1.
IPR018200. USP_CS.
IPR028889. USP_dom.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
[Graphical view]
PROSITEiPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q29RP1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPGVIPSESN GLSRGSPSKK NRLSLKFFQK KETKRALDFT DSQENEEKTS
60 70 80 90 100
EYKGSEIDQV VPAAQSSPIN CEKRENLLPF VGLNNLGNTC YLNSILQVLY
110 120 130 140 150
FCPGFKSGVK HLFNIISRKK EALKDEANQK DKGNCKEDSL ASYELICSLQ
160 170 180 190 200
SLIISVEQLQ ASFLLNPEKY TDELATQPRR LLNTLRELNP MYEGYLQHDA
210 220 230 240 250
QEVLQCILGN IQETCQLLKK EEVKNVEDLS TKVEEYQKEE MSDNNSMEMD
260 270 280 290 300
NMRHSEDYKE KLSKGNGKRK SDAEFGNMKK KVKISKEHQS SEENQRQTRS
310 320 330 340 350
KRKAAGDTLE ISHKIIPKHI SENESTRPSQ RKSKVKINWL KSAAKQPSIL
360 370 380 390 400
SKFCSMGKIA TNQGSKGHCK ENEYDLEEDL GKYENDNTTN DCELESPGNN
410 420 430 440 450
DMPVHVNEVK PINKGAEQIG FELVEKLFQG QLVLRTRCLE CESLTERRED
460 470 480 490 500
FQDISVPVQE DELSKVEENS EISPEPKTEM KTLRWAISQF ASVERIVGED
510 520 530 540 550
KYFCENCHHY TEAERSLLFD KMPEVITIHL KCFAASGLEF DCYGGGLSKI
560 570 580 590 600
NTPLLTPLKL SLEEWSTKPT NDSYGLFAVV MHSGITISSG HYTASVKVTD
610 620 630 640 650
LNSLELDKEN FVIDQTCEIG KPEPLNEEEV RGVVENYDNE EVSIRVSGNN
660 670 680 690 700
QPSKVLNKKN VEAIGLLGGQ KSKADYELYN KASNPDKVAS TALPENRNSE
710 720 730 740 750
TNNTNGTDES DSNKESSDQT GINISGFENK ISYVVQSLKE YEGKWLLFDD
760 770 780
SEVKVTEEKD FLNSLSPSTS PTSTPYLLFY KKL
Length:783
Mass (Da):88,459
Last modified:April 4, 2006 - v1
Checksum:i7910D2FC5668E87C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC114087 mRNA. Translation: AAI14088.1.
RefSeqiNP_001039689.1. NM_001046224.1.
UniGeneiBt.40485.

Genome annotation databases

GeneIDi516328.
KEGGibta:516328.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC114087 mRNA. Translation: AAI14088.1.
RefSeqiNP_001039689.1. NM_001046224.1.
UniGeneiBt.40485.

3D structure databases

ProteinModelPortaliQ29RP1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000027254.

Protein family/group databases

MEROPSiC19.019.

Proteomic databases

PaxDbiQ29RP1.
PRIDEiQ29RP1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi516328.
KEGGibta:516328.

Organism-specific databases

CTDi7398.

Phylogenomic databases

eggNOGiKOG1864. Eukaryota.
ENOG410XQ81. LUCA.
HOGENOMiHOG000154756.
HOVERGENiHBG017288.
InParanoidiQ29RP1.
KOiK11832.

Family and domain databases

CDDicd02671. Peptidase_C19O. 1 hit.
InterProiIPR001394. Peptidase_C19_UCH.
IPR033815. USP1.
IPR018200. USP_CS.
IPR028889. USP_dom.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
[Graphical view]
PROSITEiPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUBP1_BOVIN
AccessioniPrimary (citable) accession number: Q29RP1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: April 4, 2006
Last modified: November 30, 2016
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.