Q29RP1 (UBP1_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin carboxyl-terminal hydrolase 1
EC=3.4.19.12

Alternative name(s):
Deubiquitinating enzyme 1
Ubiquitin thioesterase 1
Ubiquitin-specific-processing protease 1

Gene names

Name:

USP1

Organism

Bos taurus (Bovine) [Reference proteome]

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	783 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Negative regulator of DNA damage repair which specifically deubiquitinates monoubiquitinated FANCD2. Also involved in PCNA-mediated translesion synthesis (TLS) by deubiquitinating monoubiquitinated PCNA. Has almost no deubiquitinating activity by itself and requires the interaction with WDR48 to have a high activity By similarity.
Catalytic activity	Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Subunit structure	Interacts with FANCD2 and PCNA. Interacts with WDR48 By similarity.
Subcellular location	Nucleus By similarity UniProtKB O94782.
Post-translational modification	Autocatalytic cleavage of USP1 following UV irradiation inactivates it leading to an increase in ubiquitinated PCNA, recruitment of POLH and translesion synthesis By similarity. Ubiquitinated; leading to its subsequent proteasomal degradation By similarity.
Sequence similarities	Belongs to the peptidase C19 family.

Ontologies

Keywords
Biological process	DNA damage DNA repair Ubl conjugation pathway
Cellular component	Nucleus
Molecular function	Hydrolase Protease Thiol protease
PTM	Autocatalytic cleavage Phosphoprotein Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA repair Inferred from electronic annotation. Source: UniProtKB-KW monoubiquitinated protein deubiquitination Inferred from sequence or structural similarity. Source: UniProtKB regulation of DNA repair Inferred from sequence or structural similarity. Source: UniProtKB response to UV Inferred from sequence or structural similarity. Source: UniProtKB ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	nucleus Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	ubiquitin thiolesterase activity Inferred from sequence or structural similarity. Source: UniProtKB ubiquitin-specific protease activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 783	783	Ubiquitin carboxyl-terminal hydrolase 1		PRO_0000306286
Sites
	Active site	90	1	Nucleophile By similarity UniProtKB Q93009
	Active site	591	1	Proton acceptor By similarity UniProtKB Q93009
	Site	669 – 670	2	Cleavage; by autolysis By similarity UniProtKB O94782
Amino acid modifications
	Modified residue	42	1	Phosphoserine By similarity UniProtKB O94782
	Modified residue	67	1	Phosphoserine By similarity
	Modified residue	473	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q29RP1 [UniParc]. Last modified April 4, 2006. Version 1. Checksum: 7910D2FC5668E87C	FASTA	783	88,459
	10 20 30 40 50 60 MPGVIPSESN GLSRGSPSKK NRLSLKFFQK KETKRALDFT DSQENEEKTS EYKGSEIDQV 70 80 90 100 110 120 VPAAQSSPIN CEKRENLLPF VGLNNLGNTC YLNSILQVLY FCPGFKSGVK HLFNIISRKK 130 140 150 160 170 180 EALKDEANQK DKGNCKEDSL ASYELICSLQ SLIISVEQLQ ASFLLNPEKY TDELATQPRR 190 200 210 220 230 240 LLNTLRELNP MYEGYLQHDA QEVLQCILGN IQETCQLLKK EEVKNVEDLS TKVEEYQKEE 250 260 270 280 290 300 MSDNNSMEMD NMRHSEDYKE KLSKGNGKRK SDAEFGNMKK KVKISKEHQS SEENQRQTRS 310 320 330 340 350 360 KRKAAGDTLE ISHKIIPKHI SENESTRPSQ RKSKVKINWL KSAAKQPSIL SKFCSMGKIA 370 380 390 400 410 420 TNQGSKGHCK ENEYDLEEDL GKYENDNTTN DCELESPGNN DMPVHVNEVK PINKGAEQIG 430 440 450 460 470 480 FELVEKLFQG QLVLRTRCLE CESLTERRED FQDISVPVQE DELSKVEENS EISPEPKTEM 490 500 510 520 530 540 KTLRWAISQF ASVERIVGED KYFCENCHHY TEAERSLLFD KMPEVITIHL KCFAASGLEF 550 560 570 580 590 600 DCYGGGLSKI NTPLLTPLKL SLEEWSTKPT NDSYGLFAVV MHSGITISSG HYTASVKVTD 610 620 630 640 650 660 LNSLELDKEN FVIDQTCEIG KPEPLNEEEV RGVVENYDNE EVSIRVSGNN QPSKVLNKKN 670 680 690 700 710 720 VEAIGLLGGQ KSKADYELYN KASNPDKVAS TALPENRNSE TNNTNGTDES DSNKESSDQT 730 740 750 760 770 780 GINISGFENK ISYVVQSLKE YEGKWLLFDD SEVKVTEEKD FLNSLSPSTS PTSTPYLLFY KKL

References

[1]	NIH - Mammalian Gene Collection (MGC) project Submitted (FEB-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Heart ventricle.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC114087 mRNA. Translation: AAI14088.1.
IPI	IPI00708651.
RefSeq	NP_001039689.1. NM_001046224.1.
UniGene	Bt.40485.
3D structure databases
HSSP	HSSP built from PDB template 2GFO based on UniProtKB P40818.
ProteinModelPortal	Q29RP1.
ModBase	Search...
Protein-protein interaction databases
STRING	9913.ENSBTAP00000027254.
Protein family/group databases
MEROPS	C19.019.
Proteomic databases
PRIDE	Q29RP1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	516328.
KEGG	bta:516328.
Organism-specific databases
CTD	7398.
Phylogenomic databases
eggNOG	COG5077.
HOGENOM	HOG000154756.
HOVERGEN	HBG017288.
InParanoid	Q29RP1.
KO	K11832.
OrthoDB	EOG4XSKP7.
Family and domain databases
InterPro	IPR018200. Pept_C19ubi-hydrolase_C_CS. IPR001394. Peptidase_C19. [Graphical view]
Pfam	PF00443. UCH. 1 hit. [Graphical view]
PROSITE	PS00972. UCH_2_1. 1 hit. PS00973. UCH_2_2. 1 hit. PS50235. UCH_2_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20872192.

Entry information

Entry name

UBP1_BOVIN

Accession

Primary (citable) accession number: Q29RP1

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 2, 2007
Last sequence update:	April 4, 2006
Last modified:	April 3, 2013
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program