Reviewed, UniProtKB/Swiss-Prot Q29RP1 (UBP1_BOVIN)

Names and origin

Protein names

Recommended name:
    Ubiquitin carboxyl-terminal hydrolase 1
    EC=3.1.2.15
Alternative name(s):
    Ubiquitin thioesterase 1
    Ubiquitin-specific-processing protease 1
    Deubiquitinating enzyme 1

Gene names

Name:

USP1

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	783 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Negative regulator of DNA damage repair. Autocatalytic cleavage of USP1 leads to an increase in ubiquitinated PCNA, recruitment of POLH and translesion synthesis following DNA damage. Regulates ubiquitination of FANCD2 By similarity. UniProtKB O94782
Catalytic activity	Ubiquitin C-terminal thioester + H2O = ubiquitin + a thiol. UniProtKB O94782
Subunit structure	Interacts with FANCD2 By similarity. UniProtKB O94782
Subcellular location	Nucleus By similarity. UniProtKB O94782
Post-translational modification	Ubiquitinated; leading to its subsequent proteasomal degradation By similarity. Phosphorylated upon DNA damage, probably by ATM or ATR By similarity. UniProtKB O94782
Sequence similarities	Belongs to the peptidase C19 family.

Ontologies

Keywords
Biological process	DNA damage DNA repair Ubl conjugation pathway
Cellular component	Nucleus
Molecular function	Hydrolase Protease Thiol protease
PTM	Autocatalytic cleavage Phosphoprotein Ubl conjugation
Gene Ontology (GO)
Biological process	DNA repair Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cysteine-type peptidase activity Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin thiolesterase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 783	783	Ubiquitin carboxyl-terminal hydrolase 1		PRO_0000306286
Sites
	Active site	90	1	By similarity UniProtKB Q93009
	Active site	582	1	By similarity UniProtKB Q93009
	Active site	591	1	By similarity UniProtKB Q93009
	Site	669 – 670	2	Cleavage; by autolysis By similarity UniProtKB O94782
Amino acid modifications
	Modified residue	13	1	Phosphoserine By similarity
	Modified residue	42	1	Phosphoserine By similarity UniProtKB O94782
	Modified residue	67	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q29RP1-1 [UniParc]. Last modified April 4, 2006. Version 1. Checksum: 7910D2FC5668E87C	FASTA	783	88,459
	10 20 30 40 50 60 MPGVIPSESN GLSRGSPSKK NRLSLKFFQK KETKRALDFT DSQENEEKTS EYKGSEIDQV 70 80 90 100 110 120 VPAAQSSPIN CEKRENLLPF VGLNNLGNTC YLNSILQVLY FCPGFKSGVK HLFNIISRKK 130 140 150 160 170 180 EALKDEANQK DKGNCKEDSL ASYELICSLQ SLIISVEQLQ ASFLLNPEKY TDELATQPRR 190 200 210 220 230 240 LLNTLRELNP MYEGYLQHDA QEVLQCILGN IQETCQLLKK EEVKNVEDLS TKVEEYQKEE 250 260 270 280 290 300 MSDNNSMEMD NMRHSEDYKE KLSKGNGKRK SDAEFGNMKK KVKISKEHQS SEENQRQTRS 310 320 330 340 350 360 KRKAAGDTLE ISHKIIPKHI SENESTRPSQ RKSKVKINWL KSAAKQPSIL SKFCSMGKIA 370 380 390 400 410 420 TNQGSKGHCK ENEYDLEEDL GKYENDNTTN DCELESPGNN DMPVHVNEVK PINKGAEQIG 430 440 450 460 470 480 FELVEKLFQG QLVLRTRCLE CESLTERRED FQDISVPVQE DELSKVEENS EISPEPKTEM 490 500 510 520 530 540 KTLRWAISQF ASVERIVGED KYFCENCHHY TEAERSLLFD KMPEVITIHL KCFAASGLEF 550 560 570 580 590 600 DCYGGGLSKI NTPLLTPLKL SLEEWSTKPT NDSYGLFAVV MHSGITISSG HYTASVKVTD 610 620 630 640 650 660 LNSLELDKEN FVIDQTCEIG KPEPLNEEEV RGVVENYDNE EVSIRVSGNN QPSKVLNKKN 670 680 690 700 710 720 VEAIGLLGGQ KSKADYELYN KASNPDKVAS TALPENRNSE TNNTNGTDES DSNKESSDQT 730 740 750 760 770 780 GINISGFENK ISYVVQSLKE YEGKWLLFDD SEVKVTEEKD FLNSLSPSTS PTSTPYLLFY KKL

References

[1]	NIH - Mammalian Gene Collection (MGC) project Submitted (FEB-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Heart ventricle.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC114087 mRNA. Translation: AAI14088.1.
IPI	IPI00708651.
RefSeq	NP_001039689.1.
UniGene	Bt.40485
3D structure databases
ModBase	Search...
Protein family/group databases
MEROPS	C19.019.
Genome annotation databases
Ensembl	ENSBTAG00000020451. Bos taurus. [Contig view]
GeneID	516328.
KEGG	bta:516328.
Phylogenomic databases
HOVERGEN	Q29RP1.
Enzyme and pathway databases
BRENDA	3.1.2.15. 251.
Family and domain databases
InterPro	IPR018200. Pept_C19ubi-hydrolase_C_CS. IPR001394. Peptidase_C19. [Graphical view]
Pfam	PF00443. UCH. 1 hit. [Graphical view]
PROSITE	PS00972. UCH_2_1. 1 hit. PS00973. UCH_2_2. 1 hit. PS50235. UCH_2_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UBP1_BOVIN

Accession

Primary (citable) accession number: Q29RP1

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 2, 2007
Last sequence update:	April 4, 2006
Last modified:	June 16, 2009
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)