Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q29RP1

- UBP1_BOVIN

UniProt

Q29RP1 - UBP1_BOVIN

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Ubiquitin carboxyl-terminal hydrolase 1

Gene

USP1

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Negative regulator of DNA damage repair which specifically deubiquitinates monoubiquitinated FANCD2. Also involved in PCNA-mediated translesion synthesis (TLS) by deubiquitinating monoubiquitinated PCNA. Has almost no deubiquitinating activity by itself and requires the interaction with WDR48 to have a high activity (By similarity).By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei90 – 901NucleophilePROSITE-ProRule annotation
Active sitei591 – 5911Proton acceptorPROSITE-ProRule annotation
Sitei669 – 6702Cleavage; by autolysisBy similarity

GO - Molecular functioni

  1. ubiquitin-specific protease activity Source: UniProtKB
  2. ubiquitin thiolesterase activity Source: UniProtKB

GO - Biological processi

  1. DNA repair Source: UniProtKB-KW
  2. monoubiquitinated protein deubiquitination Source: UniProtKB
  3. protein deubiquitination Source: UniProtKB
  4. regulation of DNA repair Source: UniProtKB
  5. response to UV Source: UniProtKB
  6. ubiquitin-dependent protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

DNA damage, DNA repair, Ubl conjugation pathway

Protein family/group databases

MEROPSiC19.019.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 1 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 1
Ubiquitin thioesterase 1
Ubiquitin-specific-processing protease 1
Gene namesi
Name:USP1By similarity
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Unplaced

Subcellular locationi

Nucleus By similarity

GO - Cellular componenti

  1. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 783783Ubiquitin carboxyl-terminal hydrolase 1PRO_0000306286Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei42 – 421PhosphoserineBy similarity
Modified residuei67 – 671PhosphoserineBy similarity
Modified residuei473 – 4731PhosphoserineBy similarity

Post-translational modificationi

Autocatalytic cleavage of USP1 following UV irradiation inactivates it leading to an increase in ubiquitinated PCNA, recruitment of POLH and translesion synthesis.By similarity
Ubiquitinated; leading to its subsequent proteasomal degradation.By similarity

Keywords - PTMi

Autocatalytic cleavage, Phosphoprotein, Ubl conjugation

Proteomic databases

PRIDEiQ29RP1.

Interactioni

Subunit structurei

Interacts with FANCD2 and PCNA. Interacts with WDR48 (By similarity).By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000027254.

Structurei

3D structure databases

ProteinModelPortaliQ29RP1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini81 – 783703USPAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase C19 family.Sequence Analysis
Contains 1 USP domain.Curated

Phylogenomic databases

eggNOGiCOG5077.
HOGENOMiHOG000154756.
HOVERGENiHBG017288.
InParanoidiQ29RP1.
KOiK11832.

Family and domain databases

InterProiIPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
[Graphical view]
PfamiPF00443. UCH. 2 hits.
[Graphical view]
PROSITEiPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q29RP1-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPGVIPSESN GLSRGSPSKK NRLSLKFFQK KETKRALDFT DSQENEEKTS
60 70 80 90 100
EYKGSEIDQV VPAAQSSPIN CEKRENLLPF VGLNNLGNTC YLNSILQVLY
110 120 130 140 150
FCPGFKSGVK HLFNIISRKK EALKDEANQK DKGNCKEDSL ASYELICSLQ
160 170 180 190 200
SLIISVEQLQ ASFLLNPEKY TDELATQPRR LLNTLRELNP MYEGYLQHDA
210 220 230 240 250
QEVLQCILGN IQETCQLLKK EEVKNVEDLS TKVEEYQKEE MSDNNSMEMD
260 270 280 290 300
NMRHSEDYKE KLSKGNGKRK SDAEFGNMKK KVKISKEHQS SEENQRQTRS
310 320 330 340 350
KRKAAGDTLE ISHKIIPKHI SENESTRPSQ RKSKVKINWL KSAAKQPSIL
360 370 380 390 400
SKFCSMGKIA TNQGSKGHCK ENEYDLEEDL GKYENDNTTN DCELESPGNN
410 420 430 440 450
DMPVHVNEVK PINKGAEQIG FELVEKLFQG QLVLRTRCLE CESLTERRED
460 470 480 490 500
FQDISVPVQE DELSKVEENS EISPEPKTEM KTLRWAISQF ASVERIVGED
510 520 530 540 550
KYFCENCHHY TEAERSLLFD KMPEVITIHL KCFAASGLEF DCYGGGLSKI
560 570 580 590 600
NTPLLTPLKL SLEEWSTKPT NDSYGLFAVV MHSGITISSG HYTASVKVTD
610 620 630 640 650
LNSLELDKEN FVIDQTCEIG KPEPLNEEEV RGVVENYDNE EVSIRVSGNN
660 670 680 690 700
QPSKVLNKKN VEAIGLLGGQ KSKADYELYN KASNPDKVAS TALPENRNSE
710 720 730 740 750
TNNTNGTDES DSNKESSDQT GINISGFENK ISYVVQSLKE YEGKWLLFDD
760 770 780
SEVKVTEEKD FLNSLSPSTS PTSTPYLLFY KKL
Length:783
Mass (Da):88,459
Last modified:April 4, 2006 - v1
Checksum:i7910D2FC5668E87C
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC114087 mRNA. Translation: AAI14088.1.
RefSeqiNP_001039689.1. NM_001046224.1.
UniGeneiBt.40485.

Genome annotation databases

GeneIDi516328.
KEGGibta:516328.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC114087 mRNA. Translation: AAI14088.1 .
RefSeqi NP_001039689.1. NM_001046224.1.
UniGenei Bt.40485.

3D structure databases

ProteinModelPortali Q29RP1.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9913.ENSBTAP00000027254.

Protein family/group databases

MEROPSi C19.019.

Proteomic databases

PRIDEi Q29RP1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 516328.
KEGGi bta:516328.

Organism-specific databases

CTDi 7398.

Phylogenomic databases

eggNOGi COG5077.
HOGENOMi HOG000154756.
HOVERGENi HBG017288.
InParanoidi Q29RP1.
KOi K11832.

Miscellaneous databases

NextBioi 20872192.

Family and domain databases

InterProi IPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
[Graphical view ]
Pfami PF00443. UCH. 2 hits.
[Graphical view ]
PROSITEi PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. NIH - Mammalian Gene Collection (MGC) project
    Submitted (FEB-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: HerefordImported.
    Tissue: Heart ventricleImported.

Entry informationi

Entry nameiUBP1_BOVIN
AccessioniPrimary (citable) accession number: Q29RP1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: April 4, 2006
Last modified: October 1, 2014
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3