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Protein

Pyruvate carboxylase, mitochondrial

Gene

PC

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second. Catalyzes in a tissue specific manner, the initial reactions of glucose (liver, kidney) and lipid (adipose tissue, liver, brain) synthesis from pyruvate (By similarity).By similarity

Catalytic activityi

ATP + pyruvate + HCO3- = ADP + phosphate + oxaloacetate.

Cofactori

Protein has several cofactor binding sites:
  • biotinBy similarity
  • Mn2+By similarityNote: Binds 1 Mn2+ ion per subunit.By similarity

Pathwayi: gluconeogenesis

This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.
View all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei152ATPBy similarity1
Binding sitei236ATPBy similarity1
Binding sitei271ATPBy similarity1
Active sitei328By similarity1
Metal bindingi572ManganeseBy similarity1
Binding sitei644SubstrateBy similarity1
Metal bindingi741Manganese; via carbamate groupBy similarity1
Metal bindingi771ManganeseBy similarity1
Metal bindingi773ManganeseBy similarity1
Binding sitei908SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase, Multifunctional enzyme
Biological processGluconeogenesis, Lipid biosynthesis, Lipid metabolism
LigandATP-binding, Biotin, Manganese, Metal-binding, Nucleotide-binding, Pyruvate

Enzyme and pathway databases

BRENDAi6.4.1.1 908
ReactomeiR-BTA-196780 Biotin transport and metabolism
R-BTA-70263 Gluconeogenesis
UniPathwayiUPA00138

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate carboxylase, mitochondrial (EC:6.4.1.1)
Alternative name(s):
Pyruvic carboxylase
Short name:
PCB
Gene namesi
Name:PC
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 29

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL1641351

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 20MitochondrionSequence analysisAdd BLAST20
ChainiPRO_000023986821 – 1178Pyruvate carboxylase, mitochondrialAdd BLAST1158

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei35N6-acetyllysineBy similarity1
Modified residuei39N6-acetyllysineBy similarity1
Modified residuei79N6-acetyllysine; alternateBy similarity1
Modified residuei79N6-succinyllysine; alternateBy similarity1
Modified residuei148N6-acetyllysineBy similarity1
Modified residuei152N6-acetyllysineBy similarity1
Modified residuei241N6-acetyllysineBy similarity1
Modified residuei297N6-acetyllysineBy similarity1
Modified residuei319N6-acetyllysineBy similarity1
Modified residuei434N6-acetyllysineBy similarity1
Modified residuei442N6-succinyllysineBy similarity1
Modified residuei589N6-acetyllysineBy similarity1
Modified residuei661N6-acetyllysineBy similarity1
Modified residuei717N6-acetyllysineBy similarity1
Modified residuei741N6-carboxylysineBy similarity1
Modified residuei748N6-acetyllysineBy similarity1
Modified residuei892N6-acetyllysineBy similarity1
Modified residuei969N6-acetyllysineBy similarity1
Modified residuei992N6-acetyllysineBy similarity1
Modified residuei1003PhosphothreonineBy similarity1
Modified residuei1061N6-acetyllysineBy similarity1
Modified residuei1090N6-acetyllysineBy similarity1
Modified residuei1124N6-acetyllysineBy similarity1
Modified residuei1144N6-biotinyllysinePROSITE-ProRule annotationBy similarity1

Post-translational modificationi

Acetylation of Lys-748 might play a role in catalytic activity regulation.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ29RK2
PeptideAtlasiQ29RK2
PRIDEiQ29RK2

Expressioni

Gene expression databases

BgeeiENSBTAG00000019700

Interactioni

Subunit structurei

Homotetramer. Interacts (via the biotin carboxylation domain) with SIRT4.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000030027

Structurei

3D structure databases

ProteinModelPortaliQ29RK2
SMRiQ29RK2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini36 – 486Biotin carboxylationAdd BLAST451
Domaini156 – 353ATP-graspPROSITE-ProRule annotationAdd BLAST198
Domaini563 – 832Pyruvate carboxyltransferasePROSITE-ProRule annotationAdd BLAST270
Domaini1109 – 1178Biotinyl-bindingPROSITE-ProRule annotationAdd BLAST70

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni571 – 575Substrate bindingBy similarity5

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiENOG410IU5D Eukaryota
COG1038 LUCA
GeneTreeiENSGT00900000141164
HOGENOMiHOG000282801
HOVERGENiHBG008340
InParanoidiQ29RK2
KOiK01958
OMAiGQPHGGF
OrthoDBiEOG091G00X2
TreeFamiTF300535

Family and domain databases

Gene3Di3.20.20.70, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR011761 ATP-grasp
IPR005481 BC-like_N
IPR001882 Biotin_BS
IPR011764 Biotin_carboxylation_dom
IPR005482 Biotin_COase_C
IPR000089 Biotin_lipoyl
IPR003379 Carboxylase_cons_dom
IPR005479 CbamoylP_synth_lsu-like_ATP-bd
IPR016185 PreATP-grasp_dom_sf
IPR000891 PYR_CT
IPR005930 Pyruv_COase
IPR011054 Rudment_hybrid_motif
IPR011053 Single_hybrid_motif
PfamiView protein in Pfam
PF02785 Biotin_carb_C, 1 hit
PF00289 Biotin_carb_N, 1 hit
PF00364 Biotin_lipoyl, 1 hit
PF02786 CPSase_L_D2, 1 hit
PF00682 HMGL-like, 1 hit
PF02436 PYC_OADA, 1 hit
PIRSFiPIRSF001594 Pyruv_carbox, 1 hit
SMARTiView protein in SMART
SM00878 Biotin_carb_C, 1 hit
SUPFAMiSSF51230 SSF51230, 1 hit
SSF51246 SSF51246, 1 hit
SSF52440 SSF52440, 1 hit
TIGRFAMsiTIGR01235 pyruv_carbox, 1 hit
PROSITEiView protein in PROSITE
PS50975 ATP_GRASP, 1 hit
PS50979 BC, 1 hit
PS00188 BIOTIN, 1 hit
PS50968 BIOTINYL_LIPOYL, 1 hit
PS50991 PYR_CT, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q29RK2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLKFQTVRGS LRLLAIRRTS TATAASPNVR RLEYKPIKKV MVANRGEIAI
60 70 80 90 100
RVFRACTELG IRTVAVYSEQ DTGQMHRQKA DEAYLIGRGL APVQAYLHIP
110 120 130 140 150
DIIKVAKENN VDAVHPGYGF LSERADFAQA CQDAGVRFIG PSPEVVRKMG
160 170 180 190 200
DKVEARAIAI AAGVPVVPGT DAPITSLHEA HEFSNTYGFP IIFKAAYGGG
210 220 230 240 250
GRGMRVVHSY EELEENYTRA YSEALAAFGN GALFVEKFIE KPRHIEVQIL
260 270 280 290 300
GDQYGNILHL YERDCSIQRR HQKVVEIAPA AHLDPQLRTR LTSDSVKLAK
310 320 330 340 350
QVGYENAGTV EFLVDRHGKH YFIEVNSRLQ VEHTVTEEIT DVDLVHAQIH
360 370 380 390 400
VAEGRSLPDL GLRQENIRIN GCAIQCRVTT EDPARSFQPD TGRIEVFRSG
410 420 430 440 450
EGMGIRLDNA SAFQGAVISP HYDSLLVKVI AHGKDHPTAA TKMSRALAEF
460 470 480 490 500
RVRGVKTNIP FLQNVLNNQQ FLAGTVDTQF IDENPELFQL RPAQNRAQKL
510 520 530 540 550
LHYLGHVMVN GPTTPIPVKA SPSPTDPIVP VVPIGPPPTG FRDILLREGP
560 570 580 590 600
EGFARAVRNH EGLLLMDTTF RDAHQSLLAT RVRTHDLKKI SPYVAHSFNK
610 620 630 640 650
LFSIENWGGA TFDVAMRFLY ECPWRRLQEL RELVPNIPFQ MLLRGANAVG
660 670 680 690 700
YTNYPDNVVF KFCEVAKENG MDIFRVFDSL NYLPNLLLGM EAAGSAGGVV
710 720 730 740 750
EAAISYTGDV SDPSRTKYSL QYYMGLAEEL VRAGTHILCI KDMAGLLKPT
760 770 780 790 800
ACTMLVSSLR DRFPDLPLHI HTHDTSGAGV AAMLACAHAG ADVVDVAADS
810 820 830 840 850
MSGMTSQPSM GALVACTRGT PLDTGVPLER VFDYSEYWEG ARGLYAAFDC
860 870 880 890 900
TATMKSGNSD VYENEIPGGQ YTNLHFQAHS MGLGSKFKEV KKAYVEANQM
910 920 930 940 950
LGDLIKVTPS SKIVGDLAQF MVQNGLTRAE AEAQAEELSF PRSVVEFLQG
960 970 980 990 1000
YIGIPHGGFP EPLRSKVLKD LPRVEGRPGA SLPPLDLQAL EKELTERHGE
1010 1020 1030 1040 1050
EVTPEDVLSA AMYPDVFAHF KDFTATFGPL DSLNTRLFLQ GPKIAEEFEV
1060 1070 1080 1090 1100
ELERGKTLHI KALAISDLNR AGQRQVFFEL NGQLRSILVK DTQAMKEMHF
1110 1120 1130 1140 1150
HPKALKDVKG QIGAPMPGKV IDIKVAAGAK VTKGQPLCVL SAMKMETVVT
1160 1170
SPVEGTVRKV HVTKDMTLEG DDLILEIE
Length:1,178
Mass (Da):129,698
Last modified:July 25, 2006 - v2
Checksum:i15DDD18BC7920D01
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti149M → I in AAO27903 (PubMed:15248600).Curated1
Sequence conflicti157A → T in AAO27903 (PubMed:15248600).Curated1
Sequence conflicti163G → D in AAO27903 (PubMed:15248600).Curated1
Sequence conflicti171D → N in AAO27903 (PubMed:15248600).Curated1
Sequence conflicti439A → D in AAO27903 (PubMed:15248600).Curated1
Sequence conflicti568T → A in AAI14136 (Ref. 2) Curated1
Sequence conflicti592P → L in AAI14136 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY185595 mRNA Translation: AAO27903.1
BC114135 mRNA Translation: AAI14136.1
RefSeqiNP_808815.2, NM_177946.4
XP_005227045.1, XM_005226988.3
XP_005227046.1, XM_005226989.3
XP_005227047.1, XM_005226990.3
XP_005227048.1, XM_005226991.3
XP_005227049.1, XM_005226992.3
XP_005227050.1, XM_005226993.3
XP_005227051.1, XM_005226994.3
XP_015316738.1, XM_015461252.1
XP_015316739.1, XM_015461253.1
UniGeneiBt.10147

Genome annotation databases

EnsembliENSBTAT00000026258; ENSBTAP00000026258; ENSBTAG00000019700
ENSBTAT00000030039; ENSBTAP00000030027; ENSBTAG00000019700
GeneIDi338471
KEGGibta:338471

Similar proteinsi

Entry informationi

Entry nameiPYC_BOVIN
AccessioniPrimary (citable) accession number: Q29RK2
Secondary accession number(s): Q866R1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 13, 2006
Last sequence update: July 25, 2006
Last modified: April 25, 2018
This is version 103 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health