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Protein

Pyruvate carboxylase, mitochondrial

Gene

PC

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second. Catalyzes in a tissue specific manner, the initial reactions of glucose (liver, kidney) and lipid (adipose tissue, liver, brain) synthesis from pyruvate (By similarity).By similarity

Catalytic activityi

ATP + pyruvate + HCO3- = ADP + phosphate + oxaloacetate.

Cofactori

Protein has several cofactor binding sites:
  • biotinBy similarity
  • Mn2+By similarityNote: Binds 1 Mn2+ ion per subunit.By similarity

Pathwayi: gluconeogenesis

This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.
View all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei152ATPBy similarity1
Binding sitei236ATPBy similarity1
Binding sitei271ATPBy similarity1
Active sitei328By similarity1
Metal bindingi572ManganeseBy similarity1
Binding sitei644SubstrateBy similarity1
Metal bindingi741Manganese; via carbamate groupBy similarity1
Metal bindingi771ManganeseBy similarity1
Metal bindingi773ManganeseBy similarity1
Binding sitei908SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Gluconeogenesis, Lipid biosynthesis, Lipid metabolism

Keywords - Ligandi

ATP-binding, Biotin, Manganese, Metal-binding, Nucleotide-binding, Pyruvate

Enzyme and pathway databases

BRENDAi6.4.1.1. 908.
ReactomeiR-BTA-196780. Biotin transport and metabolism.
R-BTA-70263. Gluconeogenesis.
UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate carboxylase, mitochondrial (EC:6.4.1.1)
Alternative name(s):
Pyruvic carboxylase
Short name:
PCB
Gene namesi
Name:PC
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 29

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL1641351.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 20MitochondrionSequence analysisAdd BLAST20
ChainiPRO_000023986821 – 1178Pyruvate carboxylase, mitochondrialAdd BLAST1158

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei79N6-acetyllysine; alternateBy similarity1
Modified residuei79N6-succinyllysine; alternateBy similarity1
Modified residuei297N6-acetyllysineBy similarity1
Modified residuei319N6-acetyllysineBy similarity1
Modified residuei434N6-acetyllysineBy similarity1
Modified residuei442N6-succinyllysineBy similarity1
Modified residuei661N6-acetyllysineBy similarity1
Modified residuei741N6-carboxylysineBy similarity1
Modified residuei748N6-acetyllysineBy similarity1
Modified residuei992N6-acetyllysineBy similarity1
Modified residuei1003PhosphothreonineBy similarity1
Modified residuei1061N6-acetyllysineBy similarity1
Modified residuei1090N6-acetyllysineBy similarity1
Modified residuei1124N6-acetyllysineBy similarity1
Modified residuei1144N6-biotinyllysinePROSITE-ProRule annotationBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ29RK2.
PeptideAtlasiQ29RK2.
PRIDEiQ29RK2.

Expressioni

Gene expression databases

BgeeiENSBTAG00000019700.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000030027.

Structurei

3D structure databases

ProteinModelPortaliQ29RK2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini36 – 486Biotin carboxylationAdd BLAST451
Domaini156 – 353ATP-graspPROSITE-ProRule annotationAdd BLAST198
Domaini563 – 832Pyruvate carboxyltransferasePROSITE-ProRule annotationAdd BLAST270
Domaini1109 – 1178Biotinyl-bindingPROSITE-ProRule annotationAdd BLAST70

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni571 – 575Substrate bindingBy similarity5

Sequence similaritiesi

Contains 1 ATP-grasp domain.PROSITE-ProRule annotation
Contains 1 biotin carboxylation domain.Curated
Contains 1 biotinyl-binding domain.PROSITE-ProRule annotationCurated
Contains 1 pyruvate carboxyltransferase domain.PROSITE-ProRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiENOG410IU5D. Eukaryota.
COG1038. LUCA.
GeneTreeiENSGT00550000074986.
HOGENOMiHOG000282801.
HOVERGENiHBG008340.
InParanoidiQ29RK2.
KOiK01958.
OMAiYAIQSRV.
OrthoDBiEOG091G00X2.
TreeFamiTF300535.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
3.20.20.70. 1 hit.
3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR005481. BC-like_N.
IPR001882. Biotin_BS.
IPR011764. Biotin_carboxylation_dom.
IPR005482. Biotin_COase_C.
IPR000089. Biotin_lipoyl.
IPR003379. Carboxylase_cons_dom.
IPR005479. CbamoylP_synth_lsu-like_ATP-bd.
IPR009057. Homeodomain-like.
IPR016185. PreATP-grasp_dom.
IPR000891. PYR_CT.
IPR005930. Pyruv_COase.
IPR011054. Rudment_hybrid_motif.
IPR011053. Single_hybrid_motif.
[Graphical view]
PfamiPF02785. Biotin_carb_C. 1 hit.
PF00289. Biotin_carb_N. 1 hit.
PF00364. Biotin_lipoyl. 1 hit.
PF02786. CPSase_L_D2. 1 hit.
PF00682. HMGL-like. 1 hit.
PF02436. PYC_OADA. 1 hit.
[Graphical view]
PIRSFiPIRSF001594. Pyruv_carbox. 1 hit.
SMARTiSM00878. Biotin_carb_C. 1 hit.
[Graphical view]
SUPFAMiSSF51230. SSF51230. 1 hit.
SSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR01235. pyruv_carbox. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
PS50979. BC. 1 hit.
PS00188. BIOTIN. 1 hit.
PS50968. BIOTINYL_LIPOYL. 1 hit.
PS50991. PYR_CT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q29RK2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLKFQTVRGS LRLLAIRRTS TATAASPNVR RLEYKPIKKV MVANRGEIAI
60 70 80 90 100
RVFRACTELG IRTVAVYSEQ DTGQMHRQKA DEAYLIGRGL APVQAYLHIP
110 120 130 140 150
DIIKVAKENN VDAVHPGYGF LSERADFAQA CQDAGVRFIG PSPEVVRKMG
160 170 180 190 200
DKVEARAIAI AAGVPVVPGT DAPITSLHEA HEFSNTYGFP IIFKAAYGGG
210 220 230 240 250
GRGMRVVHSY EELEENYTRA YSEALAAFGN GALFVEKFIE KPRHIEVQIL
260 270 280 290 300
GDQYGNILHL YERDCSIQRR HQKVVEIAPA AHLDPQLRTR LTSDSVKLAK
310 320 330 340 350
QVGYENAGTV EFLVDRHGKH YFIEVNSRLQ VEHTVTEEIT DVDLVHAQIH
360 370 380 390 400
VAEGRSLPDL GLRQENIRIN GCAIQCRVTT EDPARSFQPD TGRIEVFRSG
410 420 430 440 450
EGMGIRLDNA SAFQGAVISP HYDSLLVKVI AHGKDHPTAA TKMSRALAEF
460 470 480 490 500
RVRGVKTNIP FLQNVLNNQQ FLAGTVDTQF IDENPELFQL RPAQNRAQKL
510 520 530 540 550
LHYLGHVMVN GPTTPIPVKA SPSPTDPIVP VVPIGPPPTG FRDILLREGP
560 570 580 590 600
EGFARAVRNH EGLLLMDTTF RDAHQSLLAT RVRTHDLKKI SPYVAHSFNK
610 620 630 640 650
LFSIENWGGA TFDVAMRFLY ECPWRRLQEL RELVPNIPFQ MLLRGANAVG
660 670 680 690 700
YTNYPDNVVF KFCEVAKENG MDIFRVFDSL NYLPNLLLGM EAAGSAGGVV
710 720 730 740 750
EAAISYTGDV SDPSRTKYSL QYYMGLAEEL VRAGTHILCI KDMAGLLKPT
760 770 780 790 800
ACTMLVSSLR DRFPDLPLHI HTHDTSGAGV AAMLACAHAG ADVVDVAADS
810 820 830 840 850
MSGMTSQPSM GALVACTRGT PLDTGVPLER VFDYSEYWEG ARGLYAAFDC
860 870 880 890 900
TATMKSGNSD VYENEIPGGQ YTNLHFQAHS MGLGSKFKEV KKAYVEANQM
910 920 930 940 950
LGDLIKVTPS SKIVGDLAQF MVQNGLTRAE AEAQAEELSF PRSVVEFLQG
960 970 980 990 1000
YIGIPHGGFP EPLRSKVLKD LPRVEGRPGA SLPPLDLQAL EKELTERHGE
1010 1020 1030 1040 1050
EVTPEDVLSA AMYPDVFAHF KDFTATFGPL DSLNTRLFLQ GPKIAEEFEV
1060 1070 1080 1090 1100
ELERGKTLHI KALAISDLNR AGQRQVFFEL NGQLRSILVK DTQAMKEMHF
1110 1120 1130 1140 1150
HPKALKDVKG QIGAPMPGKV IDIKVAAGAK VTKGQPLCVL SAMKMETVVT
1160 1170
SPVEGTVRKV HVTKDMTLEG DDLILEIE
Length:1,178
Mass (Da):129,698
Last modified:July 25, 2006 - v2
Checksum:i15DDD18BC7920D01
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti149M → I in AAO27903 (PubMed:15248600).Curated1
Sequence conflicti157A → T in AAO27903 (PubMed:15248600).Curated1
Sequence conflicti163G → D in AAO27903 (PubMed:15248600).Curated1
Sequence conflicti171D → N in AAO27903 (PubMed:15248600).Curated1
Sequence conflicti439A → D in AAO27903 (PubMed:15248600).Curated1
Sequence conflicti568T → A in AAI14136 (Ref. 2) Curated1
Sequence conflicti592P → L in AAI14136 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY185595 mRNA. Translation: AAO27903.1.
BC114135 mRNA. Translation: AAI14136.1.
RefSeqiNP_808815.2. NM_177946.4.
XP_005227045.1. XM_005226988.3.
XP_005227046.1. XM_005226989.3.
XP_005227047.1. XM_005226990.3.
XP_005227048.1. XM_005226991.3.
XP_005227049.1. XM_005226992.3.
XP_005227050.1. XM_005226993.3.
XP_005227051.1. XM_005226994.3.
XP_015316738.1. XM_015461252.1.
XP_015316739.1. XM_015461253.1.
UniGeneiBt.10147.

Genome annotation databases

EnsembliENSBTAT00000026258; ENSBTAP00000026258; ENSBTAG00000019700.
ENSBTAT00000030039; ENSBTAP00000030027; ENSBTAG00000019700.
GeneIDi338471.
KEGGibta:338471.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY185595 mRNA. Translation: AAO27903.1.
BC114135 mRNA. Translation: AAI14136.1.
RefSeqiNP_808815.2. NM_177946.4.
XP_005227045.1. XM_005226988.3.
XP_005227046.1. XM_005226989.3.
XP_005227047.1. XM_005226990.3.
XP_005227048.1. XM_005226991.3.
XP_005227049.1. XM_005226992.3.
XP_005227050.1. XM_005226993.3.
XP_005227051.1. XM_005226994.3.
XP_015316738.1. XM_015461252.1.
XP_015316739.1. XM_015461253.1.
UniGeneiBt.10147.

3D structure databases

ProteinModelPortaliQ29RK2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000030027.

Chemistry databases

ChEMBLiCHEMBL1641351.

Proteomic databases

PaxDbiQ29RK2.
PeptideAtlasiQ29RK2.
PRIDEiQ29RK2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000026258; ENSBTAP00000026258; ENSBTAG00000019700.
ENSBTAT00000030039; ENSBTAP00000030027; ENSBTAG00000019700.
GeneIDi338471.
KEGGibta:338471.

Organism-specific databases

CTDi5091.

Phylogenomic databases

eggNOGiENOG410IU5D. Eukaryota.
COG1038. LUCA.
GeneTreeiENSGT00550000074986.
HOGENOMiHOG000282801.
HOVERGENiHBG008340.
InParanoidiQ29RK2.
KOiK01958.
OMAiYAIQSRV.
OrthoDBiEOG091G00X2.
TreeFamiTF300535.

Enzyme and pathway databases

UniPathwayiUPA00138.
BRENDAi6.4.1.1. 908.
ReactomeiR-BTA-196780. Biotin transport and metabolism.
R-BTA-70263. Gluconeogenesis.

Miscellaneous databases

PROiQ29RK2.

Gene expression databases

BgeeiENSBTAG00000019700.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
3.20.20.70. 1 hit.
3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR005481. BC-like_N.
IPR001882. Biotin_BS.
IPR011764. Biotin_carboxylation_dom.
IPR005482. Biotin_COase_C.
IPR000089. Biotin_lipoyl.
IPR003379. Carboxylase_cons_dom.
IPR005479. CbamoylP_synth_lsu-like_ATP-bd.
IPR009057. Homeodomain-like.
IPR016185. PreATP-grasp_dom.
IPR000891. PYR_CT.
IPR005930. Pyruv_COase.
IPR011054. Rudment_hybrid_motif.
IPR011053. Single_hybrid_motif.
[Graphical view]
PfamiPF02785. Biotin_carb_C. 1 hit.
PF00289. Biotin_carb_N. 1 hit.
PF00364. Biotin_lipoyl. 1 hit.
PF02786. CPSase_L_D2. 1 hit.
PF00682. HMGL-like. 1 hit.
PF02436. PYC_OADA. 1 hit.
[Graphical view]
PIRSFiPIRSF001594. Pyruv_carbox. 1 hit.
SMARTiSM00878. Biotin_carb_C. 1 hit.
[Graphical view]
SUPFAMiSSF51230. SSF51230. 1 hit.
SSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR01235. pyruv_carbox. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
PS50979. BC. 1 hit.
PS00188. BIOTIN. 1 hit.
PS50968. BIOTINYL_LIPOYL. 1 hit.
PS50991. PYR_CT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPYC_BOVIN
AccessioniPrimary (citable) accession number: Q29RK2
Secondary accession number(s): Q866R1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 13, 2006
Last sequence update: July 25, 2006
Last modified: November 2, 2016
This is version 95 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.