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Protein

Atypical kinase COQ8A, mitochondrial

Gene

COQ8A

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Atypical kinase involved in the biosynthesis of coenzyme Q, also named ubiquinone, an essential lipid-soluble electron transporter for aerobic cellular respiration. Its substrate specificity is unclear: does not show any protein kinase activity. Probably acts as a small molecule kinase, possibly a lipid kinase that phosphorylates a prenyl lipid in the ubiquinone biosynthesis pathway, as suggested by its ability to bind coenzyme Q lipid intermediates. Shows an unusual selectivity for binding ADP over ATP.By similarity

Enzyme regulationi

Autoinhibited by the N-terminal domain, containing the KxGQ motif, that completely occludes the typical substrate binding pocket. Nucleotide-binding relieves inhibition.By similarity

Pathwayi: ubiquinone biosynthesis

This protein is involved in the pathway ubiquinone biosynthesis, which is part of Cofactor biosynthesis.By similarity
View all proteins of this organism that are known to be involved in the pathway ubiquinone biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei340ATPBy similarity1
Binding sitei358ATPBy similarity1
Active sitei488Proton acceptorBy similarity1
Binding sitei493ATPBy similarity1
Binding sitei507ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi445 – 448ATPBy similarity4

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Ubiquinone biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00232.

Names & Taxonomyi

Protein namesi
Recommended name:
Atypical kinase COQ8A, mitochondrialCurated (EC:2.7.-.-By similarity)
Alternative name(s):
Chaperone activity of bc1 complex-likeBy similarity
Short name:
Chaperone-ABC1-likeBy similarity
Coenzyme Q protein 8ABy similarity
aarF domain-containing protein kinase 3By similarity
Gene namesi
Name:COQ8ABy similarity
Synonyms:ADCK3By similarity, CABC1By similarity
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 16

Subcellular locationi

  • Mitochondrion By similarity
  • Membrane Sequence analysis; Single-pass membrane protein Sequence analysisBy similarity

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei214 – 230HelicalSequence analysisAdd BLAST17

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 162MitochondrionBy similarityAdd BLAST162
ChainiPRO_0000271794163 – 648Atypical kinase COQ8A, mitochondrialAdd BLAST486

Proteomic databases

PaxDbiQ29RI0.
PRIDEiQ29RI0.

Expressioni

Gene expression databases

BgeeiENSBTAG00000021880.

Interactioni

Subunit structurei

Homodimer; homodimerizes via its transmembrane region. Interacts with the multi-subunit COQ enzyme complex, composed of at least COQ3, COQ4, COQ5, COQ6, COQ7 and COQ9.By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000029167.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini329 – 518Protein kinaseAdd BLAST190

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi276 – 279KxGQ motifBy similarity4
Motifi337 – 340AAAS motifBy similarity4

Domaini

Adopts an atypical protein kinase-like fold: while it adopts a core fold similar to that of well-characterized protein kinase-like domains, a number of features are positioned to inhibit the kinase activity: (1) an atypical AAAS motif in an alanine-rich (A-rich) loop that replaces the canonical glycine-rich (G-rich) nucleotide-binding loop and limits ATP binding by establishing an unusual selectivity for ADP and (2) an N-terminal domain, containing the KxGQ motif, that completely occludes the typical substrate binding pocket. Nucleotide-binding opens the substrate binding pocket and flips the active site from inside the hydrophobic core into a catalytically competent, solvent-exposed posture.By similarity

Sequence similaritiesi

Contains 1 protein kinase domain.Curated

Keywords - Domaini

Transit peptide, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1234. Eukaryota.
COG0661. LUCA.
GeneTreeiENSGT00550000074739.
HOGENOMiHOG000201140.
HOVERGENiHBG061318.
InParanoidiQ29RI0.
KOiK08869.
OMAiYEVKVME.
OrthoDBiEOG091G02P9.
TreeFamiTF300630.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR004147. UbiB_dom.
[Graphical view]
PfamiPF03109. ABC1. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 2 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q29RI0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAMLGDAIM LIKGFVKLTQ AAVETHLQHL GLSGELLMAV RALQSTATEQ
60 70 80 90 100
VGMVFGQVQG QETPEEYYSE SLDDPEGAFH FSGMRAESAS ADVSAASSPE
110 120 130 140 150
QSPPPWAHAA GSEGPAPAYV ASAPFREGGV LGQAASPLGR VNGRLFASPR
160 170 180 190 200
DPFSAPGLQR RVYHQDQSSM GGLTAEDIEK ARQAKARPES KPHKQALSEH
210 220 230 240 250
ARERKVPVTR IGRLANFGGL AVGLGFGALA EVAKKSLRPD DPSGKKAVLD
260 270 280 290 300
SSPFLSEANA ERIVRTLCKV RGAALKLGQM LSIQDDAFIN PHLAKIFDRV
310 320 330 340 350
RQSADFMPLK QMMKTLNNDL GPNWRDKLEY FEERPFAAAS IGQVHLARLK
360 370 380 390 400
GGREVAMKIQ YPGVAQSINS DVNNLMTVLN MSNMLPEGLF PEHLIDVLRR
410 420 430 440 450
ELALECDYQR EAACARRFRE LLKDHPFFYV PEIVDELCSP HVLTTELVSG
460 470 480 490 500
FPLDQAEGLS QEIRNEICYN ILVLCLRELF EFQFMQTDPN WSNFFYDPEL
510 520 530 540 550
HKVALLDFGA TREFDRSFTD LYIQIIRAAA NQDREAVLKK SIEMKFLTGY
560 570 580 590 600
EVKAMEDAHL DAILILGEAF ASEEPFDFGT QSTTEKIHNL IPIMLKHRLV
610 620 630 640
PPPEETYSLH RKMGGSFLIC SKLKARFPCK AMFEEAYSNY CRRQAEQQ
Length:648
Mass (Da):72,082
Last modified:April 4, 2006 - v1
Checksum:i217518E10C95C73D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC114164 mRNA. Translation: AAI14165.1.
BT026249 mRNA. Translation: ABG67088.1.
RefSeqiNP_001039884.1. NM_001046419.2.
XP_010811432.1. XM_010813130.2.
UniGeneiBt.22379.

Genome annotation databases

EnsembliENSBTAT00000029167; ENSBTAP00000029167; ENSBTAG00000021880.
GeneIDi536925.
KEGGibta:536925.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC114164 mRNA. Translation: AAI14165.1.
BT026249 mRNA. Translation: ABG67088.1.
RefSeqiNP_001039884.1. NM_001046419.2.
XP_010811432.1. XM_010813130.2.
UniGeneiBt.22379.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000029167.

Proteomic databases

PaxDbiQ29RI0.
PRIDEiQ29RI0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000029167; ENSBTAP00000029167; ENSBTAG00000021880.
GeneIDi536925.
KEGGibta:536925.

Organism-specific databases

CTDi56997.

Phylogenomic databases

eggNOGiKOG1234. Eukaryota.
COG0661. LUCA.
GeneTreeiENSGT00550000074739.
HOGENOMiHOG000201140.
HOVERGENiHBG061318.
InParanoidiQ29RI0.
KOiK08869.
OMAiYEVKVME.
OrthoDBiEOG091G02P9.
TreeFamiTF300630.

Enzyme and pathway databases

UniPathwayiUPA00232.

Gene expression databases

BgeeiENSBTAG00000021880.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR004147. UbiB_dom.
[Graphical view]
PfamiPF03109. ABC1. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiCOQ8A_BOVIN
AccessioniPrimary (citable) accession number: Q29RI0
Secondary accession number(s): Q0V8G9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 9, 2007
Last sequence update: April 4, 2006
Last modified: November 2, 2016
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.