Reviewed, UniProtKB/Swiss-Prot Q29RH3 (NUD12_BOVIN)

Names and origin

Protein names

Recommended name:
    Peroxisomal NADH pyrophosphatase NUDT12
    EC=3.6.1.22
Alternative name(s):
    Nucleoside diphosphate-linked moiety X motif 12
      Short name=Nudix motif 12

Gene names

Name:

NUDT12

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	444 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Hydrolyzes NAD(P)H to NMNH and AMP (2',5'-ADP), and diadenosine diphosphate to AMP. Has also activity towards NAD(P)+, ADP-ribose and diadenosine triphosphate. May act to regulate the concentration of peroxisomal nicotinamide nucleotide cofactors required for oxidative metabolism in this organelle By similarity.
Catalytic activity	NAD+ + H2O = AMP + NMN.
Cofactor	Magnesium or manganese By similarity.
Subcellular location	Peroxisome By similarity.
Sequence similarities	Belongs to the Nudix hydrolase family. NudC subfamily. Contains 2 ANK repeats.

Ontologies

Keywords
Cellular component	Peroxisome
Domain	ANK repeat Repeat
Ligand	Magnesium Manganese Metal-binding NAD NADP
Molecular function	Hydrolase
Gene Ontology (GO)
Cellular component	peroxisome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NAD+ diphosphatase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 444	444	Peroxisomal NADH pyrophosphatase NUDT12		PRO_0000260768
Regions
	Repeat	11 – 40	30	ANK 1
	Repeat	60 – 80	21	ANK 2
	Motif	337 – 358	22	Nudix box
	Motif	442 – 444	3	Microbody targeting signal By similarity
Sites
	Metal binding	352	1	Magnesium or manganese By similarity
	Metal binding	356	1	Magnesium or manganese By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q29RH3-1 [UniParc]. Last modified April 4, 2006. Version 1. Checksum: D9352B6AFCD6F21B	FASTA	444	50,119
	10 20 30 40 50 60 MSSVKRSLNQ EIISQFHYSA AEGDIAKLTA ILSHSPSLLN ETSENGWSAL ICDRSIVNKS 70 80 90 100 110 120 RQTALDIAKF WGYKHIANLL ANAKGGKKPW FLTNEVEECE NYFSKTLLDR KSEKRNNSDW 130 140 150 160 170 180 LLAKESHPAT VYILFSDLNP LVTLGGNKES FQQPEVRLCQ LNYTDIKDYL AQPEKITLIF 190 200 210 220 230 240 LGVELEMKKE FFNYAGEISK EEEDGLVAWF ALGIDTVAAE EFKQRHENCY FLHPPMPALL 250 260 270 280 290 300 QLKEKEAGVV AQARSVLAWH SRYKFCPTCG NATKIEEGGY KRVCLKEDCP SLHGVHNTSY 310 320 330 340 350 360 PRVDPVVIMQ VIHPDGTKCL LGRQKRFPPG MFTCLAGFIE PGETIEDAVR REVEEESGVK 370 380 390 400 410 420 VGHVQYVSCQ PWPMPSSLMI GCLAVAVSTE IKVDKNEIED ARWFTREQVV DVLTKGKQQA 430 440 FFVPPSRAIA HQLIKHWIGM NPNL

References

[1]	NIH - Mammalian Gene Collection (MGC) project Submitted (FEB-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Heart ventricle.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC114173 mRNA. Translation: AAI14174.1.
IPI	IPI00694800.
RefSeq	NP_001040073.1.
UniGene	Bt.17805
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	617720.
KEGG	bta:617720.
Phylogenomic databases
HOVERGEN	Q29RH3.
Enzyme and pathway databases
BRENDA	3.6.1.22. 251.
Family and domain databases
InterPro	IPR002110. ANK. IPR015375. NADH_PPase-like_N. IPR000086. NUDIX_hydrolase_core. IPR015376. Znr_NADH_PPase. [Graphical view]
Gene3D	G3DSA:1.25.40.20. ANK. 1 hit. G3DSA:3.90.79.10. NUDIX_hydrolase. 1 hit.
Pfam	PF00293. NUDIX. 1 hit. PF09296. NUDIX-like. 1 hit. PF09297. zf-NADH-PPase. 1 hit. [Graphical view]
PROSITE	PS50297. ANK_REP_REGION. False negative. PS50088. ANK_REPEAT. False negative. PS00893. NUDIX. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NUD12_BOVIN

Accession

Primary (citable) accession number: Q29RH3

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2006
Last sequence update:	April 4, 2006
Last modified:	June 16, 2009
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)