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Q29PG5

- MOC32_DROPS

UniProt

Q29PG5 - MOC32_DROPS

Protein

Adenylyltransferase and sulfurtransferase MOCS3-2

Gene

GA12041

Organism
Drosophila pseudoobscura pseudoobscura (Fruit fly)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 53 (01 Oct 2014)
      Sequence version 1 (04 Apr 2006)
      Previous versions | rss
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    Functioni

    Plays a central role in 2-thiolation of mcm5S2U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Also essential during biosynthesis of the molybdenum cofactor. Acts by mediating the C-terminal thiocarboxylation of sulfur carriers URM1 and MOCS2A. Its N-terminus first activates URM1 and MOCS2A as acyl-adenylates (-COAMP), then the persulfide sulfur on the catalytic cysteine is transferred to URM1 and MOCS2A to form thiocarboxylation (-COSH) of their C-terminus. The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as nucleophile towards URM1 and MOCS2A. Subsequently, a transient disulfide bond is formed. Does not use thiosulfate as sulfur donor; NFS1 probably acting as a sulfur donor for thiocarboxylation reactions.UniRule annotation

    Catalytic activityi

    ATP + [molybdopterin-synthase sulfur-carrier protein]-Gly-Gly = diphosphate + [molybdopterin-synthase sulfur-carrier protein]-Gly-Gly-AMP.UniRule annotation
    [Molybdopterin-synthase sulfur-carrier protein]-Gly-Gly-AMP + [cysteine desulfurase]-S-sulfanyl-L-cysteine = AMP + [molybdopterin-synthase sulfur-carrier protein]-Gly-NH-CH(2)-C(O)SH + cysteine desulfurase.UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei99 – 991ATP; via amide nitrogenUniRule annotation
    Binding sitei120 – 1201ATPUniRule annotation
    Binding sitei144 – 1441ATPUniRule annotation
    Metal bindingi229 – 2291ZincUniRule annotation
    Metal bindingi232 – 2321ZincUniRule annotation
    Active sitei246 – 2461Glycyl thioester intermediate; for adenylyltransferase activityUniRule annotation
    Metal bindingi304 – 3041ZincUniRule annotation
    Metal bindingi307 – 3071ZincUniRule annotation
    Active sitei408 – 4081Cysteine persulfide intermediate; for sulfurtransferase activityUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi127 – 1315ATPUniRule annotation
    Nucleotide bindingi188 – 1892ATPUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW
    3. molybdopterin-synthase adenylyltransferase activity Source: UniProtKB-EC
    4. molybdopterin-synthase sulfurtransferase activity Source: UniProtKB-EC
    5. thiosulfate sulfurtransferase activity Source: UniProtKB

    GO - Biological processi

    1. enzyme active site formation via L-cysteine persulfide Source: UniProtKB-HAMAP
    2. Mo-molybdopterin cofactor biosynthetic process Source: UniProtKB
    3. tRNA wobble position uridine thiolation Source: InterPro

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Molybdenum cofactor biosynthesis, tRNA processing

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    UniPathwayiUPA00344.
    UPA00988.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Adenylyltransferase and sulfurtransferase MOCS3-2UniRule annotation
    Alternative name(s):
    Molybdenum cofactor synthesis protein 3-2UniRule annotation
    Including the following 2 domains:
    Molybdopterin-synthase adenylyltransferase 2UniRule annotation (EC:2.7.7.80UniRule annotation)
    Alternative name(s):
    Adenylyltransferase MOCS3-2UniRule annotation
    Sulfur carrier protein MOCS2A adenylyltransferase 2UniRule annotation
    Molybdopterin-synthase sulfurtransferase 2UniRule annotation (EC:2.8.1.11UniRule annotation)
    Alternative name(s):
    Sulfur carrier protein MOCS2A sulfurtransferase 2UniRule annotation
    Sulfurtransferase MOCS3-2UniRule annotation
    Gene namesi
    ORF Names:GA12041
    OrganismiDrosophila pseudoobscura pseudoobscura (Fruit fly)
    Taxonomic identifieri46245 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
    ProteomesiUP000001819: Partially assembled WGS sequence

    Organism-specific databases

    FlyBaseiFBgn0072089. Dpse\GA12041.

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytosol Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 451451Adenylyltransferase and sulfurtransferase MOCS3-2PRO_0000369208Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei60 – 601PhosphothreonineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Interactioni

    Protein-protein interaction databases

    STRINGi7237.FBpp0279121.

    Structurei

    3D structure databases

    ProteinModelPortaliQ29PG5.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini353 – 44997RhodaneseUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    In the N-terminal section; belongs to the HesA/MoeB/ThiF family. UBA4 subfamily.UniRule annotation
    Contains 1 rhodanese domain.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0476.
    InParanoidiQ29PG5.
    KOiK11996.
    OMAiTDYVFFC.
    OrthoDBiEOG776SQ3.
    PhylomeDBiQ29PG5.

    Family and domain databases

    Gene3Di3.40.250.10. 1 hit.
    3.40.50.720. 1 hit.
    HAMAPiMF_03049. MOCS3_Uba4.
    InterProiIPR028885. MOCS3/Uba4.
    IPR007901. MoeZ_MoeB.
    IPR009036. Molybdenum_cofac_synth_MoeB.
    IPR016040. NAD(P)-bd_dom.
    IPR001763. Rhodanese-like_dom.
    IPR000594. ThiF_NAD_FAD-bd.
    [Graphical view]
    PfamiPF05237. MoeZ_MoeB. 1 hit.
    PF00581. Rhodanese. 1 hit.
    PF00899. ThiF. 1 hit.
    [Graphical view]
    SMARTiSM00450. RHOD. 1 hit.
    [Graphical view]
    SUPFAMiSSF69572. SSF69572. 1 hit.
    PROSITEiPS50206. RHODANESE_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q29PG5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MIDSEALESE RVKLKRDIAD LRANLNRKEQ CLRELEAAIA AGEDSDEAEE    50
    SSNDMPTPQT KLTNDDIARY SRQLILQDFG VQGQLKLKNS SVLIVGMGGL 100
    GCPAAQYLVA AGCGHLGLID YDEVERSNLH RQILHSEHRC GMSKAESARI 150
    ALLELNSHCQ IRCHSRLINS MNAMHIIRPY DVVLDCSDNV ATRYLLNDAC 200
    VMLRKPLVSG SALKMDGQLT VYGYGQGPCY RCIYPVPPPP EAVTNCGDGG 250
    VLGAVTGIIG AMQALEAIKV IIGLGDVMSG RLLIFDGSSF MFRNIRIRTK 300
    RPNCHVCSAQ PLITELIDYE MFCGMHATDK DNPLDLLEPD QRLEVKEYHQ 350
    KLQSQPHLLL DVRPPAEFEI CQLPRSINVP LSEILDDSYL KRFAKQLEDK 400
    ELPIVLLCRR GNDSQIAVQH ITNRFPAHSI RDLVGGLHAW TGSVDATFPI 450
    Y 451
    Length:451
    Mass (Da):50,086
    Last modified:April 4, 2006 - v1
    Checksum:i31E6FDE5E76538E3
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CH379058 Genomic DNA. Translation: EAL34328.1.
    RefSeqiXP_001357259.1. XM_001357223.2.

    Genome annotation databases

    EnsemblMetazoaiFBtr0280683; FBpp0279121; FBgn0072089.
    GeneIDi4817985.
    KEGGidpo:Dpse_GA12041.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CH379058 Genomic DNA. Translation: EAL34328.1 .
    RefSeqi XP_001357259.1. XM_001357223.2.

    3D structure databases

    ProteinModelPortali Q29PG5.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 7237.FBpp0279121.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai FBtr0280683 ; FBpp0279121 ; FBgn0072089 .
    GeneIDi 4817985.
    KEGGi dpo:Dpse_GA12041.

    Organism-specific databases

    FlyBasei FBgn0072089. Dpse\GA12041.

    Phylogenomic databases

    eggNOGi COG0476.
    InParanoidi Q29PG5.
    KOi K11996.
    OMAi TDYVFFC.
    OrthoDBi EOG776SQ3.
    PhylomeDBi Q29PG5.

    Enzyme and pathway databases

    UniPathwayi UPA00344 .
    UPA00988 .

    Family and domain databases

    Gene3Di 3.40.250.10. 1 hit.
    3.40.50.720. 1 hit.
    HAMAPi MF_03049. MOCS3_Uba4.
    InterProi IPR028885. MOCS3/Uba4.
    IPR007901. MoeZ_MoeB.
    IPR009036. Molybdenum_cofac_synth_MoeB.
    IPR016040. NAD(P)-bd_dom.
    IPR001763. Rhodanese-like_dom.
    IPR000594. ThiF_NAD_FAD-bd.
    [Graphical view ]
    Pfami PF05237. MoeZ_MoeB. 1 hit.
    PF00581. Rhodanese. 1 hit.
    PF00899. ThiF. 1 hit.
    [Graphical view ]
    SMARTi SM00450. RHOD. 1 hit.
    [Graphical view ]
    SUPFAMi SSF69572. SSF69572. 1 hit.
    PROSITEi PS50206. RHODANESE_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution."
      Richards S., Liu Y., Bettencourt B.R., Hradecky P., Letovsky S., Nielsen R., Thornton K., Hubisz M.J., Chen R., Meisel R.P., Couronne O., Hua S., Smith M.A., Zhang P., Liu J., Bussemaker H.J., van Batenburg M.F., Howells S.L.
      , Scherer S.E., Sodergren E., Matthews B.B., Crosby M.A., Schroeder A.J., Ortiz-Barrientos D., Rives C.M., Metzker M.L., Muzny D.M., Scott G., Steffen D., Wheeler D.A., Worley K.C., Havlak P., Durbin K.J., Egan A., Gill R., Hume J., Morgan M.B., Miner G., Hamilton C., Huang Y., Waldron L., Verduzco D., Clerc-Blankenburg K.P., Dubchak I., Noor M.A.F., Anderson W., White K.P., Clark A.G., Schaeffer S.W., Gelbart W.M., Weinstock G.M., Gibbs R.A.
      Genome Res. 15:1-18(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: MV2-25 / Tucson 14011-0121.94.

    Entry informationi

    Entry nameiMOC32_DROPS
    AccessioniPrimary (citable) accession number: Q29PG5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 14, 2009
    Last sequence update: April 4, 2006
    Last modified: October 1, 2014
    This is version 53 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programDrosophila annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme, Reference proteome

    Documents

    1. Drosophila
      Drosophila: entries, gene names and cross-references to FlyBase
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3