Q29JP8 (FICD_DROPS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Adenosine monophosphate-protein transferase FICD homolog
EC=2.7.7.n1

Gene names

ORF Names:

GA21854

Organism

Drosophila pseudoobscura pseudoobscura (Fruit fly) [Reference proteome]

Taxonomic identifier

46245 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Eremoneura › Cyclorrhapha › Schizophora › Acalyptratae › Ephydroidea › Drosophilidae › Drosophilinae › Drosophilini › Drosophilina › Drosophiliti › Drosophila › Sophophora › obscura group › pseudoobscura subgroup › Drosophila pseudoobscura

Protein attributes

Sequence length	508 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Adenylyltransferase that mediates the addition of adenosine 5'-monophosphate (AMP) to specific residues of target proteins By similarity.
Catalytic activity	ATP + [protein] = diphosphate + [protein]-AMP.
Enzyme regulation	Adenylyltransferase activity is inhibited by the inhibitory helix present at the N-terminus: Glu-261 binds ATP and competes with ATP-binding at Arg-400, thereby preventing adenylyltransferase activity. Activation dissociates ATP-binding from Glu-261, allowing ordered binding of the entire ATP moiety with the alpha-phosphate in an orientation that is productive for accepting an incoming target hydroxyl side chain By similarity.
Subcellular location	Membrane; Single-pass membrane protein Potential.
Domain	The fido domain mediates the adenylyltransferase activity By similarity.
Sequence similarities	Belongs to the fic family. Contains 1 fido domain. Contains 2 TPR repeats.

Ontologies

Keywords
Cellular component	Membrane
Domain	Repeat TPR repeat Transmembrane Transmembrane helix
Ligand	ATP-binding Nucleotide-binding
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein adenylyltransferase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 508	508	Adenosine monophosphate-protein transferase FICD homolog		PRO_0000381788
Regions
	Transmembrane	48 – 70	23	Helical; Potential
	Repeat	132 – 165	34	TPR 1
	Repeat	166 – 200	35	TPR 2
	Domain	311 – 446	136	Fido
	Nucleotide binding	287 – 288	2	ATP By similarity
	Nucleotide binding	394 – 396	3	ATP By similarity
	Motif	257 – 262	6	Inhibitory (S/T)XXXE(G/N) motif
Sites
	Binding site	261	1	ATP By similarity
	Binding site	400	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q29JP8 [UniParc]. Last modified October 14, 2008. Version 2. Checksum: 95E147D1C96A3533	FASTA	508	57,776
	10 20 30 40 50 60 MAMTILHASE KVNAEAEATT CPPTEKVKEE QQQQEQLQHS KTSKRVQFYR FALFFIAGSF 70 80 90 100 110 120 AAFSFHALTS SSSWRLRQLH HLPNAHYLQT REEFAVYSVE ELNAFKEFYD KSISDSVGAS 130 140 150 160 170 180 YSEAEQTNIK EALGALRLAQ DMHLSGKDDK ASRLFEHALA LAPKHPEVLL RYGEFLEHNQ 190 200 210 220 230 240 RNIVLADQYY FQALTLCPSN SEALANRQRT AEVVQTLDER RLQSLDSKRD ALSAIHESSS 250 260 270 280 290 300 ALRRAKKEAY FQHIYHSVGI EGNTMTLAQT RSILETRMAV DGKSIDEHNE ILGMDLAMKY 310 320 330 340 350 360 INASLVQKLE ITIKDILELH RRVLGHVDPI EGGEFRRNQV YVGGHVPPGP GDLALLMQRF 370 380 390 400 410 420 ERWLNSEHSS SLHPVNYAAY AHYKLVHIHP FIDGNGRTSR LLMNTLLMRA GYPPVIIPKQ 430 440 450 460 470 480 QRSKYYHFLK LANEGDIRPF VRFIADCTEK TLDLYLWATS DLPQQIPMLI QTESEAGEQL 490 500 AQMRSPHISA QSASIPEFYE FSGSGFQP

References

[1]

"Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution." Richards S., Liu Y., Bettencourt B.R., Hradecky P., Letovsky S., Nielsen R., Thornton K., Hubisz M.J., Chen R., Meisel R.P., Couronne O., Hua S., Smith M.A., Zhang P., Liu J., Bussemaker H.J., van Batenburg M.F., Howells S.L. , Scherer S.E., Sodergren E., Matthews B.B., Crosby M.A., Schroeder A.J., Ortiz-Barrientos D., Rives C.M., Metzker M.L., Muzny D.M., Scott G., Steffen D., Wheeler D.A., Worley K.C., Havlak P., Durbin K.J., Egan A., Gill R., Hume J., Morgan M.B., Miner G., Hamilton C., Huang Y., Waldron L., Verduzco D., Clerc-Blankenburg K.P., Dubchak I., Noor M.A.F., Anderson W., White K.P., Clark A.G., Schaeffer S.W., Gelbart W.M., Weinstock G.M., Gibbs R.A. Genome Res. 15:1-18(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MV2-25 / Tucson 14011-0121.94.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CH379062 Genomic DNA. Translation: EAL32913.2.
RefSeq	XP_001355854.2. XM_001355818.2.
3D structure databases
ProteinModelPortal	Q29JP8.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0287194; FBpp0285632; FBgn0081839.
GeneID	4816233.
KEGG	dpo:Dpse_GA21854.
Organism-specific databases
FlyBase	FBgn0081839. Dpse\GA21854.
Phylogenomic databases
eggNOG	COG3177.
InParanoid	Q29JP8.
OrthoDB	EOG4XSJ5D.
Family and domain databases
Gene3D	1.10.3290.10. 1 hit. 1.25.40.10. 1 hit.
InterPro	IPR003812. Fido. IPR013026. TPR-contain_dom. IPR011990. TPR-like_helical. [Graphical view]
Pfam	PF02661. Fic. 1 hit. [Graphical view]
SUPFAM	SSF140931. SSF140931. 1 hit.
PROSITE	PS51459. FIDO. 1 hit. PS50005. TPR. False negative. PS50293. TPR_REGION. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FICD_DROPS

Accession

Primary (citable) accession number: Q29JP8

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 1, 2009
Last sequence update:	October 14, 2008
Last modified:	May 29, 2013
This is version 48 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project