Reviewed, UniProtKB/Swiss-Prot Q29IM7 (CASP8_DROPS)

Names and origin

Protein names

Recommended name:
    Caspase-8
    EC=3.4.22.61
Alternative name(s):
    Death-related Ced-3/NEDD2-like protein
Cleaved into the following 2 chains:
    1- Recommended name:
            Caspase-8 subunit p15
    2- Recommended name:
            Caspase-8 subunit p10

Gene names

Name:	Dredd
ORF Names:	GA20387

Organism

Drosophila pseudoobscura pseudoobscura (Fruit fly) [Complete proteome]

Taxonomic identifier

46245 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	511 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Effector of the programmed cell death (PCD) activators rpr, grim and W. May play an apoptotic role in the germline as well as soma. Role in immune response, required to resist Gram-negative bacterial infections by regulating Dpt. BG4 interacts with Dredd, BG4 promotes cleavage of Dredd and is necessary and sufficient for enhancing Dredd-induced apoptosis By similarity. UniProtKB Q8IRY7
Catalytic activity	Strict requirement for Asp at position P1 and has a preferred cleavage sequence of (Leu/Asp/Val)-Glu-Thr-Asp-|-(Gly/Ser/Ala).
Subunit structure	Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 15 kDa (caspase-8 subunit p15) and a 10 kDa (caspase-8 subunit p10) subunit. Interacts with the N-terminus of Dredd By similarity.
Subcellular location	Cytoplasm By similarity. UniProtKB Q14790
Sequence similarities	Belongs to the peptidase C14A family.
Sequence caution	The sequence EAL32626.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Apoptosis Immune response
Cellular component	Cytoplasm
Molecular function	Hydrolase Protease Thiol protease
PTM	Zymogen
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	apoptosis Inferred from sequence or structural similarity. Source: UniProtKB defense response to Gram-negative bacterium Inferred from sequence or structural similarity. Source: UniProtKB immune response Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	apoptotic protease activator activity Inferred from sequence or structural similarity. Source: UniProtKB cysteine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro protein binding Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Propeptide	1 – 242	242	By similarity UniProtKB Q8IRY7		PRO_0000271414
	Chain	243 – 405	163	Caspase-8 subunit p15		PRO_0000271415
	Propeptide	406 – 415	10	By similarity UniProtKB Q8IRY7		PRO_0000271416
	Chain	416 – 511	96	Caspase-8 subunit p10		PRO_0000271417
Sites
	Active site	352	1	By similarity UniProtKB Q14790
	Active site	393	1	By similarity UniProtKB Q14790

Sequences

	Sequence		Length	Mass (Da)
	Q29IM7-1 [UniParc]. Last modified April 4, 2006. Version 1. Checksum: 5DA4C77BB7E2C11C	FASTA	511	57,576
	10 20 30 40 50 60 MSGHNILTQL ESIDVLDLPY VERDLNFAQL VSLSFLLYGD EHSTATYILQ KLLVLARATA 70 80 90 100 110 120 SDWPHSDILS QYAKSKPQTW RKYLVEALCI IGARQVLRKL GLCWQELRMH YLPHVGSIRV 130 140 150 160 170 180 HIHPLLKSLY TICEELTLAQ RGRMVLDIKE KNSVGDPLRF YDAEYLEIFL LDWLTRRLIR 190 200 210 220 230 240 LGDFNANGSD VQLLIEYFKF NDLHAQATLL VDTVNAYATS SCSPATPNVL LPMDGNGIAN 250 260 270 280 290 300 ESSSPTAAAS RPRAKNALQL SRENAGIALI INQQEFYRDA NDDYKVYLPP IELPLRNGTD 310 320 330 340 350 360 MDKQRLTNVF SMLGYKVEAH DNLDHLSMLH HIRQACKRSL LHESLVVCIL SHGFEDAVYG 370 380 390 400 410 420 ANSVALRISD IENVLCSYEN LYEKPKLVVI QACQQEDKEN NALPYKINAS TKSPCQYLNM 430 440 450 460 470 480 LRAMSTVPGF PALRHTQTGS WFIQSLCDAI VEHSNSDHIA DILTIVINNV ANKRGNKNET 490 500 510 MVPWTGGALR QHVYFPRTSA TDKVVPDSPG L

References

[1]

"Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution." Richards S., Liu Y., Bettencourt B.R., Hradecky P., Letovsky S., Nielsen R., Thornton K., Hubisz M.J., Chen R., Meisel R.P., Couronne O., Hua S., Smith M.A., Zhang P., Liu J., Bussemaker H.J., van Batenburg M.F., Howells S.L. , Scherer S.E., Sodergren E., Matthews B.B., Crosby M.A., Schroeder A.J., Ortiz-Barrientos D., Rives C.M., Metzker M.L., Muzny D.M., Scott G., Steffen D., Wheeler D.A., Worley K.C., Havlak P., Durbin K.J., Egan A., Gill R., Hume J., Morgan M.B., Miner G., Hamilton C., Huang Y., Waldron L., Verduzco D., Clerc-Blankenburg K.P., Dubchak I., Noor M.A.F., Anderson W., White K.P., Clark A.G., Schaeffer S.W., Gelbart W.M., Weinstock G.M., Gibbs R.A. Genome Res. 15:1-18(2005) [PubMed: 15632085] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MV2-25.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CH379063 Genomic DNA. Translation: EAL32626.1. Sequence problems.
RefSeq	XP_001355567.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4816060.
KEGG	dpo:Dpse_GA20387.
Organism-specific databases
FlyBase	FBgn0080382. Dpse\GA20387.
Phylogenomic databases
OMA	Q29IM7. HIADILT.
Enzyme and pathway databases
BRENDA	3.4.22.61. 190795.
Family and domain databases
InterPro	IPR011600. Pept_C14_cat. IPR001309. Pept_C14_ICE_p20. IPR002138. Pept_C14_p10. IPR002398. Pept_C14_p45. IPR015917. Pept_C14_p45_core. [Graphical view]
PANTHER	PTHR10454. Pept_C14_p45. 1 hit.
Pfam	PF00656. Peptidase_C14. 1 hit. [Graphical view]
PRINTS	PR00376. IL1BCENZYME.
SMART	SM00115. CASc. 1 hit. [Graphical view]
PROSITE	PS50207. CASPASE_P10. 1 hit. PS50208. CASPASE_P20. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CASP8_DROPS

Accession

Primary (citable) accession number: Q29IM7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 9, 2007
Last sequence update:	April 4, 2006
Last modified:	May 5, 2009
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project