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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

GA17913

Organism
Drosophila pseudoobscura pseudoobscura (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of N7-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N7-methylguanine(46) in tRNA.UniRule annotation

Pathwayi: N(7)-methylguanine-tRNA biosynthesis

This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei70S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Binding sitei148S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Active sitei151UniRule annotation1

GO - Molecular functioni

Keywordsi

Molecular functionMethyltransferase, RNA-binding, Transferase, tRNA-binding
Biological processtRNA processing
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
ORF Names:GA17913
OrganismiDrosophila pseudoobscura pseudoobscura (Fruit fly)
Taxonomic identifieri46245 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000001819 Componenti: Partially assembled WGS sequence

Organism-specific databases

FlyBaseiFBgn0077922. Dpse\GA17913.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003705731 – 247tRNA (guanine-N(7)-)-methyltransferaseAdd BLAST247

Expressioni

Gene expression databases

BgeeiFBgn0077922.

Interactioni

Protein-protein interaction databases

STRINGi7237.FBpp0284887.

Structurei

3D structure databases

SMRiQ29I16.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni93 – 94S-adenosyl-L-methionine bindingUniRule annotation2
Regioni128 – 129S-adenosyl-L-methionine bindingUniRule annotation2
Regioni226 – 228S-adenosyl-L-methionine bindingUniRule annotation3

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

InParanoidiQ29I16.
KOiK03439.
OMAiIGMEIRV.

Family and domain databases

HAMAPiMF_03055. tRNA_methyltr_TrmB_euk. 1 hit.
InterProiView protein in InterPro
IPR029063. SAM-dependent_MTases.
IPR025763. Trm8_euk.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
PANTHERiPTHR23417. PTHR23417. 1 hit.
PTHR23417:SF20. PTHR23417:SF20. 1 hit.
PfamiView protein in Pfam
PF02390. Methyltransf_4. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiView protein in PROSITE
PS51625. SAM_MT_TRMB. 1 hit.

Sequencei

Sequence statusi: Complete.

Q29I16-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVTTTAENEV LTSTSAVTGL PQKRYYRQRA HSNPIADHCF DYPARPEDVD
60 70 80 90 100
WRSLYPSIRA DQEVSFADIG CGYGGFLVTL GELFPEKISI GMEIRVKVSD
110 120 130 140 150
YVLDRITALR QKSSGTGAYQ NIACLRTNAM KYLPNYFRKG QLEKMFFLYP
160 170 180 190 200
DPHFKRAKHK WRIINQALLS EYAYVLRKGG LVYTMTDVED LHNWIVTHMD
210 220 230 240
EHPLYERLDE EEANTDPITP KLYQSSEEGA KVVRNKGDHF LAIFRRL
Length:247
Mass (Da):28,537
Last modified:October 14, 2008 - v2
Checksum:iF7A61C33120A9D48
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH379064 Genomic DNA. Translation: EAL31590.2.
RefSeqiXP_001354537.2. XM_001354501.3.

Genome annotation databases

EnsemblMetazoaiFBtr0286449; FBpp0284887; FBgn0077922.
GeneIDi4814934.
KEGGidpo:Dpse_GA17913.

Similar proteinsi

Entry informationi

Entry nameiTRMB_DROPS
AccessioniPrimary (citable) accession number: Q29I16
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: October 14, 2008
Last modified: August 30, 2017
This is version 64 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families