Reviewed, UniProtKB/Swiss-Prot Q29I03 (T23O_DROPS)

Names and origin

Protein names

Recommended name:
    Tryptophan 2,3-dioxygenase
      Short name=TDO
    EC=1.13.11.11
Alternative name(s):
    Tryptophan pyrrolase
      Short name=Tryptophanase
    Tryptophan oxygenase
      Short name=TRPO
      Short name=TO
    Tryptamin 2,3-dioxygenase
    Protein vermilion

Gene names

Name:	v
ORF Names:	GA15272

Organism

Drosophila pseudoobscura pseudoobscura (Fruit fly) [Complete proteome]

Taxonomic identifier

46245 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	380 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the oxidative cleavage of the L-tryptophan (L-Trp) pyrrole ring By similarity.
Catalytic activity	L-tryptophan + O2 = N-formyl-L-kynurenine.
Cofactor	Binds 2 heme groups per tetramer By similarity.
Pathway	Amino-acid degradation; L-tryptophan degradation via kynurenine pathway; L-kynurenine from L-tryptophan: step 1/2. Pigment biosynthesis; ommochrome biosynthesis.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the tryptophan 2,3-dioxygenase family.

Ontologies

Keywords
Biological process	Tryptophan catabolism
Ligand	Heme Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW tryptophan catabolic process to kynurenine Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	heme binding Inferred from sequence or structural similarity. Source: UniProtKB tryptophan 2,3-dioxygenase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 380	380	Tryptophan 2,3-dioxygenase		PRO_0000360879
Regions
	Region	27 – 31	5	Substrate binding By similarity
	Region	57 – 61	5	Substrate binding By similarity
Sites
	Metal binding	313	1	Iron (heme axial ligand) By similarity
	Binding site	128	1	Substrate By similarity
	Binding site	135	1	Heme By similarity
	Binding site	328	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q29I03-1 [UniParc]. Last modified April 4, 2006. Version 1. Checksum: 8BB09A89BFFE9E54	FASTA	380	44,298
	10 20 30 40 50 60 MSCPYAGNGN DHDDAAVPLS TEVGKIYGEY LMLDKLLDAQ CMLSTEDKRP VHDEHLFIIT 70 80 90 100 110 120 HQAYELWFKQ IIFEFDSIRD MLDAEVIDET KTLEIVKRLN RVVLILKLLV DQVPILETMT 130 140 150 160 170 180 PLDFMDFRKY LAPASGFQSL QFRLIENKLG VLTEQRVRYN QKYSDVFGGD EQALSAIRSS 190 200 210 220 230 240 EYEPSLLELV QRWLERTPGL EESGFNFWKK FQQSVDQFLA AQVEIAMEEP VEQAKNYRLM 250 260 270 280 290 300 DIEKRREVYH SIFDPAVHEA LVKRGDRRFS HRALQGAIMI TFYRDEPRFS QPHQLLTLLM 310 320 330 340 350 360 DIDSLITKWR YNHVIMVQRM IGSQQLGTGG SSGYQYLRST LSDRYKVFLD LFNLSTFLIP 370 380 REAIPPLDET IRKKLVHKSV

References

[1]

"Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution." Richards S., Liu Y., Bettencourt B.R., Hradecky P., Letovsky S., Nielsen R., Thornton K., Hubisz M.J., Chen R., Meisel R.P., Couronne O., Hua S., Smith M.A., Zhang P., Liu J., Bussemaker H.J., van Batenburg M.F., Howells S.L. , Scherer S.E., Sodergren E., Matthews B.B., Crosby M.A., Schroeder A.J., Ortiz-Barrientos D., Rives C.M., Metzker M.L., Muzny D.M., Scott G., Steffen D., Wheeler D.A., Worley K.C., Havlak P., Durbin K.J., Egan A., Gill R., Hume J., Morgan M.B., Miner G., Hamilton C., Huang Y., Waldron L., Verduzco D., Clerc-Blankenburg K.P., Dubchak I., Noor M.A.F., Anderson W., White K.P., Clark A.G., Schaeffer S.W., Gelbart W.M., Weinstock G.M., Gibbs R.A. Genome Res. 15:1-18(2005) [PubMed: 15632085] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MV2-25.

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Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CH379064 Genomic DNA. Translation: EAL31603.1.
RefSeq	XP_001354550.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4814467.
KEGG	dpo:Dpse_GA15272.
Organism-specific databases
FlyBase	FBgn0075296. Dpse\GA15272.
Phylogenomic databases
OMA	MKLILHE.
Family and domain databases
InterPro	IPR004981. Trp_2_3_dOase. [Graphical view]
PANTHER	PTHR10138. Trp_2_3_dOase. 1 hit.
Pfam	PF03301. Trp_dioxygenase. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

T23O_DROPS

Accession

Primary (citable) accession number: Q29I03

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 20, 2009
Last sequence update:	April 4, 2006
Last modified:	November 3, 2009
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project