Q29HG0 (PGAM5_DROPS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Serine/threonine-protein phosphatase Pgam5, mitochondrial
EC=3.1.3.16

Alternative name(s):
Phosphoglycerate mutase family member 5 homolog

Gene names

Name:	Pgam5
ORF Names:	GA13269

Organism

Drosophila pseudoobscura pseudoobscura (Fruit fly) [Reference proteome]

Taxonomic identifier

46245 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Eremoneura › Cyclorrhapha › Schizophora › Acalyptratae › Ephydroidea › Drosophilidae › Drosophilinae › Drosophilini › Drosophilina › Drosophiliti › Drosophila › Sophophora › obscura group › pseudoobscura subgroup › Drosophila pseudoobscura

Protein attributes

Sequence length	289 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Displays phosphatase activity for serine/threonine residues, and dephosphorylates and activates Pk92B kinase. Has apparently no phosphoglycerate mutase activity By similarity.
Catalytic activity	A phosphoprotein + H2O = a protein + phosphate.
Subunit structure	Interacts with Pk92B/ASK1 By similarity.
Subcellular location	Mitochondrion outer membrane; Single-pass membrane protein By similarity.
Sequence similarities	Belongs to the phosphoglycerate mutase family. BPG-dependent PGAM subfamily.

Ontologies

Keywords
Cellular component	Membrane Mitochondrion Mitochondrion outer membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein dephosphorylation Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrial outer membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	phosphoprotein phosphatase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 289	289	Serine/threonine-protein phosphatase Pgam5, mitochondrial		PRO_0000288790
Regions
	Transmembrane	7 – 23	17	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	Q29HG0 [UniParc]. Last modified April 4, 2006. Version 1. Checksum: 7F67EB09A3BFC81B	FASTA	289	33,069
	10 20 30 40 50 60 MRKFTAFACG TGAGLLTFYL TKLNEPKAAV HNSWTRSEKP VDPCALWDHN WDLRDPKSLV 70 80 90 100 110 120 KPVKNDLSQE QNRYNSELEK VVPKHARHII LIRHGEYLDV GDTDETHHLT ERGREQAKYT 130 140 150 160 170 180 GKRLCELGIK WDKVIASTMV RAQETADIIL NEIDYEKAKV KNCAFLREGA PIPPQPPVGH 190 200 210 220 230 240 WKPEASQFFR DGARIEAAFR RYFYRAYPDQ TKDSYTLLVG HGNVIRYFVC RALQFPPEAW 250 260 270 280 LRISINHASI TWLTISPSGN VSIKYLGDTG FMPVNHLTNR IPRAAKNVV

References

[1]

"Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution." Richards S., Liu Y., Bettencourt B.R., Hradecky P., Letovsky S., Nielsen R., Thornton K., Hubisz M.J., Chen R., Meisel R.P., Couronne O., Hua S., Smith M.A., Zhang P., Liu J., Bussemaker H.J., van Batenburg M.F., Howells S.L. , Scherer S.E., Sodergren E., Matthews B.B., Crosby M.A., Schroeder A.J., Ortiz-Barrientos D., Rives C.M., Metzker M.L., Muzny D.M., Scott G., Steffen D., Wheeler D.A., Worley K.C., Havlak P., Durbin K.J., Egan A., Gill R., Hume J., Morgan M.B., Miner G., Hamilton C., Huang Y., Waldron L., Verduzco D., Clerc-Blankenburg K.P., Dubchak I., Noor M.A.F., Anderson W., White K.P., Clark A.G., Schaeffer S.W., Gelbart W.M., Weinstock G.M., Gibbs R.A. Genome Res. 15:1-18(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MV2-25 / Tucson 14011-0121.94.

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Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CH379064 Genomic DNA. Translation: EAL31798.1.
RefSeq	XP_001354743.1. XM_001354707.2.
3D structure databases
ProteinModelPortal	Q29HG0.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0284485; FBpp0282923; FBgn0073306.
GeneID	4815173.
KEGG	dpo:Dpse_GA13269.
Organism-specific databases
FlyBase	FBgn0073306. Dpse\GA13269.
Phylogenomic databases
eggNOG	NOG71348.
InParanoid	Q29HG0.
KO	K15637.
OMA	DTGFMPV.
OrthoDB	EOG447D98.
Family and domain databases
InterPro	IPR013078. His_Pase_superF_clade-1. [Graphical view]
Pfam	PF00300. His_Phos_1. 1 hit. [Graphical view]
SMART	SM00855. PGAM. 1 hit. [Graphical view]
PROSITE	PS00175. PG_MUTASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PGAM5_DROPS

Accession

Primary (citable) accession number: Q29HG0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	April 4, 2006
Last modified:	April 3, 2013
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project