Q29FC9 (OTU1_DROPS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin thioesterase OTU1
EC=3.4.19.12

Gene names

ORF Names:

GA18292

Organism

Drosophila pseudoobscura pseudoobscura (Fruit fly) [Reference proteome]

Taxonomic identifier

46245 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Eremoneura › Cyclorrhapha › Schizophora › Acalyptratae › Ephydroidea › Drosophilidae › Drosophilinae › Drosophilini › Drosophilina › Drosophiliti › Drosophila › Sophophora › obscura group › pseudoobscura subgroup › Drosophila pseudoobscura

Protein attributes

Sequence length	358 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Hydrolase that can remove conjugated ubiquitin from proteins and may therefore play an important regulatory role at the level of protein turnover by preventing degradation By similarity.
Catalytic activity	Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Sequence similarities	Contains 1 C2H2-type zinc finger. Contains 1 OTU domain. Contains 1 ubiquitin-like domain.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Domain	Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Protease Thiol protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular amino acid metabolic process Inferred from sequence or structural similarity. Source: UniProtKB proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	cysteine-type peptidase activity Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 358	358	Ubiquitin thioesterase OTU1		PRO_0000282363
Regions
	Domain	5 – 87	83	Ubiquitin-like
	Domain	161 – 285	125	OTU
	Zinc finger	328 – 352	25	C2H2-type
Sites
	Active site	169	1	By similarity
	Active site	172	1	Nucleophile By similarity
	Active site	352	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q29FC9 [UniParc]. Last modified April 4, 2006. Version 1. Checksum: 87FCB64C93082855	FASTA	358	39,210
	10 20 30 40 50 60 MTGSFSVKLK SKKGQFIVKD LNQNTTLGEL KTRIAQATAI QELQLHVLVG YPPKPLDLSE 70 80 90 100 110 120 NRENQNLKTV GINSGETLIV EEKAGAAGPT STPLASGSGS STMEDDEALA RRLQAEEDAE 130 140 150 160 170 180 HLRQVSSGGS IETGALNIVQ SLEPVISPEE SGPNGNFNGI LLKKVVPADN SCLFTSIRFV 190 200 210 220 230 240 LNGKVDNEGS EMMRHIIAQE VSADTQQYND AVLGKSNSDY CAWIQKADSW GGAIEVSILS 250 260 270 280 290 300 NYYGIEIDVV DIQNAIINRF GEDKNFGLRV FLLFDGIHYD PLYMETQQNS VPATIFPVEE 310 320 330 340 350 MGVYQQAEQI ANEAKSSRQF TNVDKFTLRC MDCDVMLVGQ GQAQEHAKKT GHENFEEI

References

[1]

"Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution." Richards S., Liu Y., Bettencourt B.R., Hradecky P., Letovsky S., Nielsen R., Thornton K., Hubisz M.J., Chen R., Meisel R.P., Couronne O., Hua S., Smith M.A., Zhang P., Liu J., Bussemaker H.J., van Batenburg M.F., Howells S.L. , Scherer S.E., Sodergren E., Matthews B.B., Crosby M.A., Schroeder A.J., Ortiz-Barrientos D., Rives C.M., Metzker M.L., Muzny D.M., Scott G., Steffen D., Wheeler D.A., Worley K.C., Havlak P., Durbin K.J., Egan A., Gill R., Hume J., Morgan M.B., Miner G., Hamilton C., Huang Y., Waldron L., Verduzco D., Clerc-Blankenburg K.P., Dubchak I., Noor M.A.F., Anderson W., White K.P., Clark A.G., Schaeffer S.W., Gelbart W.M., Weinstock G.M., Gibbs R.A. Genome Res. 15:1-18(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MV2-25 / Tucson 14011-0121.94.

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Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CH379067 Genomic DNA. Translation: EAL31326.1.
RefSeq	XP_001354273.1. XM_001354237.2.
3D structure databases
ProteinModelPortal	Q29FC9.
ModBase	Search...
Protein-protein interaction databases
STRING	7237.FBpp0278050.
Protein family/group databases
MEROPS	C85.007.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0279612; FBpp0278050; FBgn0078297.
GeneID	4814165.
KEGG	dpo:Dpse_GA18292.
Organism-specific databases
FlyBase	FBgn0078297. Dpse\GA18292.
Phylogenomic databases
eggNOG	COG5539.
InParanoid	Q29FC9.
KO	K13719.
OMA	FHACAYV.
OrthoDB	EOG4R229M.
Family and domain databases
InterPro	IPR003323. OTU. [Graphical view]
Pfam	PF02338. OTU. 1 hit. [Graphical view]
PROSITE	PS50802. OTU. 1 hit. PS00299. UBIQUITIN_1. False negative. PS50053. UBIQUITIN_2. False negative. PS00028. ZINC_FINGER_C2H2_1. 1 hit. PS50157. ZINC_FINGER_C2H2_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

OTU1_DROPS

Accession

Primary (citable) accession number: Q29FC9

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 3, 2007
Last sequence update:	April 4, 2006
Last modified:	April 3, 2013
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project