Reviewed, UniProtKB/Swiss-Prot Q29C43 (NCASE_DROPS)

Names and origin

Protein names

Recommended name:
    Neutral ceramidase
      Short name=N-CDase
      Short name=NCDase
    EC=3.5.1.23
Alternative name(s):
    Acylsphingosine deacylase
    N-acylsphingosine amidohydrolase

Gene names

Name:	CDase
ORF Names:	GA13191

Organism

Drosophila pseudoobscura pseudoobscura (Fruit fly) [Complete proteome]

Taxonomic identifier

46245 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	704 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Hydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid at an optimal pH of 6.5-7.5. Acts as a key regulator of sphingolipid signaling metabolites by generating sphingosine at the cell surface By similarity.
Catalytic activity	N-acylsphingosine + H2O = a carboxylate + sphingosine.
Subcellular location	Secreted By similarity.
Post-translational modification	N-glycosylated By similarity.
Sequence similarities	Belongs to the neutral ceramidase family.

Ontologies

Keywords
Biological process	Lipid metabolism Sphingolipid metabolism
Cellular component	Secreted
Domain	Signal
Molecular function	Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	sphingolipid metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ceramidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Potential
	Chain	24 – 704	681	Neutral ceramidase		PRO_0000247107
Sites
	Active site	276	1	Nucleophile By similarity
Amino acid modifications
	Glycosylation	230	1	N-linked (GlcNAc...) Potential
	Glycosylation	362	1	N-linked (GlcNAc...) Potential
	Glycosylation	550	1	N-linked (GlcNAc...) Potential
	Glycosylation	598	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q29C43-1 [UniParc]. Last modified April 4, 2006. Version 1. Checksum: 6131255E84E15F38	FASTA	704	78,092
	10 20 30 40 50 60 MAISKIAFLA LIALSGLCGL ASATYKVGVG RADITGPPVE INFMGYANIK QVGRGIHTRV 70 80 90 100 110 120 FARAFVVEDE KGNRVAFVSA DAGMMGYGLK REVIKRLQAR YGNLYHTDNV AISGTHTHGA 130 140 150 160 170 180 PGGFLMHLLY DISILGFVPQ TFEVMAQGLY LCIKRATDNL VDGRIFLSKT TVLNVNINRS 190 200 210 220 230 240 PTSYLRNPEE ERAQYEHDTD KTLTQLRFVD LENNLLGAFN WYAVHATSMN NTNRLVTSDN 250 260 270 280 290 300 VGYAALLLEK EYNPNKMPGK GKFVGAFCSS NLGDVSPNIM GPKCSISGNE CDLLTSRCPA 310 320 330 340 350 360 GEGECFASGP GRDMVESTQI LGQRLADAAL GLLNEQSQES TAREVTGDVR FIHQFVDMPN 370 380 390 400 410 420 YNGSAYNPLS RKIDKIRGCQ PAMGYSFAAG TTDGPGAFSF EQGTTTDNPM WNFVRDFIAT 430 440 450 460 470 480 PTQEDIKCHE PKPILLATGR ATFPYEWQPK IVSDQLLKIG DVIIAAVPCE FTTMAGRRLR 490 500 510 520 530 540 NQIRAAASAA GGLDTEVIIA GLTNIYTSYT VTPEEYQAQR YEAASTIFGP HTHSIYMDVF 550 560 570 580 590 600 ERLTKALMRN ETVEPGPSPP YMNDVMLSLN TGVLFDGHPI NTDFGYVKTQ PEKEYGINDT 610 620 630 640 650 660 VKVTYISGNP RNNLFTEKTY FTVERKINED RWKVAYTDAS WETKMIWHRT NTILGFSDLE 670 680 690 700 IYWNISPQTL PGVYRIRHSG EYKYILGGKY PYEGLSHSFT VKED

References

[1]

"Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution." Richards S., Liu Y., Bettencourt B.R., Hradecky P., Letovsky S., Nielsen R., Thornton K., Hubisz M.J., Chen R., Meisel R.P., Couronne O., Hua S., Smith M.A., Zhang P., Liu J., Bussemaker H.J., van Batenburg M.F., Howells S.L. , Scherer S.E., Sodergren E., Matthews B.B., Crosby M.A., Schroeder A.J., Ortiz-Barrientos D., Rives C.M., Metzker M.L., Muzny D.M., Scott G., Steffen D., Wheeler D.A., Worley K.C., Havlak P., Durbin K.J., Egan A., Gill R., Hume J., Morgan M.B., Miner G., Hamilton C., Huang Y., Waldron L., Verduzco D., Clerc-Blankenburg K.P., Dubchak I., Noor M.A.F., Anderson W., White K.P., Clark A.G., Schaeffer S.W., Gelbart W.M., Weinstock G.M., Gibbs R.A. Genome Res. 15:1-18(2005) [PubMed: 15632085] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MV2-25.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CM000070 Genomic DNA. Translation: EAL26803.1.
RefSeq	XP_001357669.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4800384.
KEGG	dpo:Dpse_GA13191.
Organism-specific databases
FlyBase	FBgn0073228. Dpse\GA13191.
Phylogenomic databases
OMA	Q29C43. LNFTQGT.
Enzyme and pathway databases
BRENDA	3.5.1.23. 190795.
Family and domain databases
InterPro	IPR006823. Ceramidase_alk. [Graphical view]
PANTHER	PTHR12670. Ceramidase_alk. 1 hit.
Pfam	PF04734. Ceramidase_alk. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NCASE_DROPS

Accession

Primary (citable) accession number: Q29C43

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2006
Last sequence update:	April 4, 2006
Last modified:	June 16, 2009
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project