Q29AU6 (RUMI_DROPS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
O-glucosyltransferase rumi
EC=2.4.1.-

Gene names

Name:	rumi
ORF Names:	GA16050

Organism

Drosophila pseudoobscura pseudoobscura (Fruit fly) [Reference proteome]

Taxonomic identifier

46245 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Eremoneura › Cyclorrhapha › Schizophora › Acalyptratae › Ephydroidea › Drosophilidae › Drosophilinae › Drosophilini › Drosophilina › Drosophiliti › Drosophila › Sophophora › obscura group › pseudoobscura subgroup › Drosophila pseudoobscura

Protein attributes

Sequence length	409 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Has O-glucosyltransferase activity: catalyzes the reaction that attaches glucose through an O-glycosidic linkage to a conserved serine residue in epidermal growth factor-like repeats. Regulates Notch signaling by glucosylating Notch in the ER, glucosylation is required for the correct folding and cleavage of Notch By similarity. UniProtKB Q8T045
Pathway	Protein modification; protein glycosylation.
Subcellular location	Endoplasmic reticulum lumen By similarity UniProtKB Q8T045.
Sequence similarities	Belongs to the glycosyltransferase 90 family.
Sequence caution	The sequence EAL27253.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Notch signaling pathway
Cellular component	Endoplasmic reticulum
Domain	Signal
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	Notch signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of Notch signaling pathway Inferred from sequence or structural similarity. Source: UniProtKB protein glycosylation in endoplasmic reticulum Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	endoplasmic reticulum lumen Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	glucosyltransferase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	Potential
	Chain	21 – 409	389	O-glucosyltransferase rumi		PRO_0000342688
Regions
	Motif	406 – 409	4	Prevents secretion from ER

Sequences

	Sequence		Length	Mass (Da)
	Q29AU6 [UniParc]. Last modified April 4, 2006. Version 1. Checksum: EC695CBD0699DCE3	FASTA	409	47,314
	10 20 30 40 50 60 MLINVVLIIL LVGLNGKASG QNQGLCSPDE NASCADSSDT QDEFSFNILR KIKKALASYQ 70 80 90 100 110 120 PCSSDANDAN CSCHAAVIKS DLAPYKATGV SRQMIESSAR YGTRYKIYEK RLYREENCMF 130 140 150 160 170 180 PARCQGIEHF LLPLVATLPD MDLVINTRDY PQINMAWGNG AQGPILSFSK TKDHRDIMYP 190 200 210 220 230 240 AWTFWAGGPA TKLHPRGIGR WDLMREKLEK RAAAIPWSQK RELGFFRGSR TSDERDSLIL 250 260 270 280 290 300 LSRRNPELVE AQYTKNQGWK SPKDTLDAPP AGEVSFEDHC KYKYLFNFRG VAASFRLKHL 310 320 330 340 350 360 FLCQSLVFHV GDEWQEFFYD QLKPWVHYVP LKNYPSQQEY EELLTFFRKN DALAQEIAQR 370 380 390 400 GRDFIWQHLR MKDIKCYWRR LLKSYVKLLT YEVQPEDQLI HIQPAKDEL

References

[1]

"Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution." Richards S., Liu Y., Bettencourt B.R., Hradecky P., Letovsky S., Nielsen R., Thornton K., Hubisz M.J., Chen R., Meisel R.P., Couronne O., Hua S., Smith M.A., Zhang P., Liu J., Bussemaker H.J., van Batenburg M.F., Howells S.L. , Scherer S.E., Sodergren E., Matthews B.B., Crosby M.A., Schroeder A.J., Ortiz-Barrientos D., Rives C.M., Metzker M.L., Muzny D.M., Scott G., Steffen D., Wheeler D.A., Worley K.C., Havlak P., Durbin K.J., Egan A., Gill R., Hume J., Morgan M.B., Miner G., Hamilton C., Huang Y., Waldron L., Verduzco D., Clerc-Blankenburg K.P., Dubchak I., Noor M.A.F., Anderson W., White K.P., Clark A.G., Schaeffer S.W., Gelbart W.M., Weinstock G.M., Gibbs R.A. Genome Res. 15:1-18(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MV2-25 / Tucson 14011-0121.94.

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Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CM000070 Genomic DNA. Translation: EAL27253.1. Sequence problems.
RefSeq	XP_001358116.1. XM_001358079.2.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0285031; FBpp0283469; FBgn0076066.
GeneID	4800928.
KEGG	dpo:Dpse_GA16050.
Organism-specific databases
FlyBase	FBgn0076066. Dpse\GA16050.
Phylogenomic databases
eggNOG	NOG248922.
InParanoid	Q29AU6.
KO	K13667.
OMA	CANDETL.
OrthoDB	EOG4RN8QS.
Enzyme and pathway databases
UniPathway	UPA00378.
Family and domain databases
InterPro	IPR006598. LipoPS_modifying. [Graphical view]
Pfam	PF05686. Glyco_transf_90. 1 hit. [Graphical view]
SMART	SM00672. CAP10. 1 hit. [Graphical view]
PROSITE	PS00014. ER_TARGET. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RUMI_DROPS

Accession

Primary (citable) accession number: Q29AU6

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 2008
Last sequence update:	April 4, 2006
Last modified:	May 1, 2013
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project