Q298P4 (PDE6_DROPS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
cGMP-specific 3',5'-cyclic phosphodiesterase
EC=3.1.4.35

Gene names

Name:	Pde6
ORF Names:	GA20950

Organism

Drosophila pseudoobscura pseudoobscura (Fruit fly)

Taxonomic identifier

46245 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	1267 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Has a role regulating cGMP transport in Malpighian tubule principal cells By similarity. UniProtKB Q9VFI9
Catalytic activity	Guanosine 3',5'-cyclic phosphate + H2O = guanosine 5'-phosphate. UniProtKB Q9VFI9
Cofactor	Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions By similarity.
Subunit structure	Interacts with PrBP By similarity. UniProtKB Q9VFI9
Subcellular location	Cell membrane; Lipid-anchor; Cytoplasmic side By similarity UniProtKB Q9VFI9.
Sequence similarities	Belongs to the cyclic nucleotide phosphodiesterase family. Contains 2 GAF domains.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Repeat
Ligand	Metal-binding cGMP
Molecular function	Hydrolase
PTM	Lipoprotein Methylation Prenylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cGMP metabolic process Inferred from sequence or structural similarity. Source: UniProtKB signal transduction Inferred from electronic annotation. Source: InterPro
Cellular component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3',5'-cyclic-GMP phosphodiesterase activity Inferred from sequence or structural similarity. Source: UniProtKB metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1264	1264	cGMP-specific 3',5'-cyclic phosphodiesterase UniProtKB Q9VFI9		PRO_0000363694
	Propeptide	1265 – 1267	3	Removed in mature form By similarity UniProtKB Q9VFI9		PRO_0000363695
Regions
	Domain	285 – 437	153	GAF 1
	Domain	469 – 653	185	GAF 2
	Compositional bias	24 – 78	55	Ala-rich
	Compositional bias	925 – 945	21	Val-rich
Sites
	Active site	759	1	Proton donor By similarity
	Metal binding	763	1	Divalent metal cation 1 By similarity
	Metal binding	799	1	Divalent metal cation 1 By similarity
	Metal binding	800	1	Divalent metal cation 1 By similarity
	Metal binding	800	1	Divalent metal cation 2 By similarity
	Metal binding	1015	1	Divalent metal cation 1 By similarity
Amino acid modifications
	Modified residue	1264	1	Cysteine methyl ester By similarity UniProtKB Q9VFI9
	Lipidation	1264	1	S-farnesyl cysteine By similarity UniProtKB Q9VFI9

Sequences

	Sequence		Length	Mass (Da)
	Q298P4 [UniParc]. Last modified October 14, 2008. Version 2. Checksum: F2265C74FA0ED90F	FASTA	1,267	140,810
	10 20 30 40 50 60 MHDLGTRQRP LSSSSSSNMT DVSAAAGGAT APAETAATSS SASKPLTNGA NKTSTAMAAP 70 80 90 100 110 120 TATPTTAATA SGAAEAGAIT SVAGISNQVK LEHHHHRQSN NNRPVAPYPP VPAAKPKPTP 130 140 150 160 170 180 TPESKFKSTS REVDVALRPT PARSSTISPG VSIHTQTIQQ ESSSAKPGMS SSSSSAQQDV 190 200 210 220 230 240 DEVARLFEEK PEAFEKWLTE RAPPEALSRL QEFIESRKPL KRPSVTSDLF QQWMSASPTV 250 260 270 280 290 300 QQKSPRSLSN SSASSTLPEC RRHLMDLDEG ELFMELIRDV ANELDIDVLC HKILVNVGLL 310 320 330 340 350 360 THADRGSLFL AKGTPHNKYL VAKLFDVTQK TALKDAVTRA SAEEIIIPFG IGIAGMVAQT 370 380 390 400 410 420 KQMINIKEAY KDARFNCEID LKTGYKTNAI LCMPICNYEG DIIGVAQIIN KTNGCMEFDE 430 440 450 460 470 480 HDVEIFRRYL TFCGIGIQNA QLFEMSVQEY RRNQILLNLA RSIFEEQNNL ECLVTKIMTE 490 500 510 520 530 540 ARELLNCERC SVFLVDLDCC EASHLEKIIE KPNQPEQRPT RAIMPGDSFD EKKMRNRFTV 550 560 570 580 590 600 LFELGGEYQA ASVSRPSKTE LSTSTLAQIA QFVATTGQTV NICDVHEWVR DHNQIRAESE 610 620 630 640 650 660 IDSTQAILCM PIVNAQKVVI GVAQLINKAN GVPFTESDAS IFEAFAIFCG LGIHNTQMYE 670 680 690 700 710 720 NACKLMAKQK VALECLSYHA TASQDQTEKL TQDAIAEAES YNLYSFTFTD FELVDDDTCR 730 740 750 760 770 780 AVLRMFLQCN LVSQFQIPYD VLCRWVLSVR KNYRPVKYHN WRHALNVAQT MFAMLKTGKM 790 800 810 820 830 840 ERFMTDLEIL GLLVACLCHD LDHRGTNNAF QTKTESPLAI LYTTSTMEHH HFDQCVMILN 850 860 870 880 890 900 SEGNNIFQPR TTRTFLLLAR RFLRFDLLPF RAFTRVLRPL VRPLLELDVL ELPDPSRIFL 910 920 930 940 950 960 LTVYLRFVTR TIFLPPPEEE EEDEVVDSVV LVVASVVVVV AASVVLAAEL DVEALSPEDY 970 980 990 1000 1010 1020 RSVMKTVESA ILSTDLAMYF KKRNAFLELV ENGEFDWQGE EKKDLLCGMM MTACDVSAIA 1030 1040 1050 1060 1070 1080 KPWEVQHKVA KLVADEFFDQ GDLEKLQLNT QPVAMMDRER KDELPKMQVG FIDVICLPLY 1090 1100 1110 1120 1130 1140 RVLCDTFPWI TPLYEGTLEN RRNWQDLAEK VEMGLTWIDH DTIDKPVEEF AGCADEEIKD 1150 1160 1170 1180 1190 1200 IEFTVTTLNC NQQAQHGAGA GGDDSHTPEH QRSGSRLSMK KTGALGKAVR SKLSKTLYNS 1210 1220 1230 1240 1250 1260 MDGSKPKTSL KLLESHVSED MDDKSPTSPS QPHAGGSVGR MSASSSTSSA GTVVDKSKKR SKLCSLL

References

[1]

"Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution." Richards S., Liu Y., Bettencourt B.R., Hradecky P., Letovsky S., Nielsen R., Thornton K., Hubisz M.J., Chen R., Meisel R.P., Couronne O., Hua S., Smith M.A., Zhang P., Liu J., Bussemaker H.J., van Batenburg M.F., Howells S.L. , Scherer S.E., Sodergren E., Matthews B.B., Crosby M.A., Schroeder A.J., Ortiz-Barrientos D., Rives C.M., Metzker M.L., Muzny D.M., Scott G., Steffen D., Wheeler D.A., Worley K.C., Havlak P., Durbin K.J., Egan A., Gill R., Hume J., Morgan M.B., Miner G., Hamilton C., Huang Y., Waldron L., Verduzco D., Clerc-Blankenburg K.P., Dubchak I., Noor M.A.F., Anderson W., White K.P., Clark A.G., Schaeffer S.W., Gelbart W.M., Weinstock G.M., Gibbs R.A. Genome Res. 15:1-18(2005) [PubMed: 15632085] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MV2-25 / Tucson 14011-0121.94.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CM000070 Genomic DNA. Translation: EAL27911.2.
RefSeq	XP_001358768.2. XM_001358731.2.
3D structure databases
HSSP	HSSP built from PDB template 2H40 based on UniProtKB O76074.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4801723.
GenomeReviews	Gene locus Pde6 in contig CM000070_GR.
KEGG	dpo:Dpse_GA20950.
Organism-specific databases
FlyBase	FBgn0080939. Dpse\GA20950.
Phylogenomic databases
InParanoid	Q298P4.
OrthoDB	EOG41RN8T.
Family and domain databases
InterPro	IPR003018. GAF. IPR003607. Metal-dep_PHydrolase_HD_dom. IPR023088. PDEase. IPR002073. PDEase_catalytic_dom. IPR023174. PDEase_CS. [Graphical view]
Gene3D	G3DSA:1.10.1300.10. PDEase_catalytic_dom. 2 hits.
KO	K13763.
Pfam	PF01590. GAF. 2 hits. PF00233. PDEase_I. 2 hits. [Graphical view]
PRINTS	PR00387. PDIESTERASE1.
SMART	SM00065. GAF. 2 hits. SM00471. HDc. 1 hit. [Graphical view]
PROSITE	PS00126. PDEASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PDE6_DROPS

Accession

Primary (citable) accession number: Q298P4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 10, 2009
Last sequence update:	October 14, 2008
Last modified:	November 16, 2011
This is version 36 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project