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Q298P4 (PDE6_DROPS) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 49. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
cGMP-specific 3',5'-cyclic phosphodiesterase

EC=3.1.4.35
Gene names
Name:Pde6
ORF Names:GA20950
OrganismDrosophila pseudoobscura pseudoobscura (Fruit fly) [Reference proteome]
Taxonomic identifier46245 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora

Protein attributes

Sequence length1110 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Has a role regulating cGMP transport in Malpighian tubule principal cells By similarity. UniProtKB Q9VFI9

Catalytic activity

Guanosine 3',5'-cyclic phosphate + H2O = guanosine 5'-phosphate. UniProtKB Q9VFI9

Cofactor

Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions By similarity.

Subunit structure

Interacts with PrBP By similarity. UniProtKB Q9VFI9

Subcellular location

Cell membrane; Lipid-anchor; Cytoplasmic side By similarity UniProtKB Q9VFI9.

Sequence similarities

Belongs to the cyclic nucleotide phosphodiesterase family.

Contains 2 GAF domains.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 11071107cGMP-specific 3',5'-cyclic phosphodiesterase
PRO_0000363694
Propeptide1108 – 11103Removed in mature form By similarity
PRO_0000363695

Regions

Domain233 – 385153GAF 1
Domain417 – 601185GAF 2
Compositional bias6 – 6055Ala-rich

Sites

Active site7071Proton donor By similarity
Metal binding7111Divalent metal cation 1 By similarity
Metal binding7471Divalent metal cation 1 By similarity
Metal binding7481Divalent metal cation 1 By similarity
Metal binding7481Divalent metal cation 2 By similarity
Metal binding8581Divalent metal cation 1 By similarity

Amino acid modifications

Modified residue11071Cysteine methyl ester By similarity
Lipidation11071S-farnesyl cysteine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q298P4 [UniParc].

Last modified October 3, 2012. Version 3.
Checksum: 4FB8DECD51B085A6

FASTA1,110123,338
        10         20         30         40         50         60 
MTDVSAAAGG ATAPAETAAT SSSASKPLTN GANKTSTAMA APTATPTTAA TASGAAEAGA 

        70         80         90        100        110        120 
ITSVAGISNQ VKLEHHHHRQ SNNNRPVAPY PPVPAAKPKP TPTPESKFKS TSREPGMSSS 

       130        140        150        160        170        180 
SSSAQQDVDE VARLFEEKPE AFEKWLTERA PPEALSRLQE FIESRKPLKR PSVTSDLFQQ 

       190        200        210        220        230        240 
WMSASPTVQQ KSPRSLSNSS ASSTLPECRR HLMDLDEGEL FMELIRDVAN ELDIDVLCHK 

       250        260        270        280        290        300 
ILVNVGLLTH ADRGSLFLAK GTPHNKYLVA KLFDVTQKTA LKDAVTRASA EEIIIPFGIG 

       310        320        330        340        350        360 
IAGMVAQTKQ MINIKEAYKD ARFNCEIDLK TGYKTNAILC MPICNYEGDI IGVAQIINKT 

       370        380        390        400        410        420 
NGCMEFDEHD VEIFRRYLTF CGIGIQNAQL FEMSVQEYRR NQILLNLARS IFEEQNNLEC 

       430        440        450        460        470        480 
LVTKIMTEAR ELLNCERCSV FLVDLDCCEA SHLEKIIEKP NQPEQRPTRA IMPGDSFDEK 

       490        500        510        520        530        540 
KMRNRFTVLF ELGGEYQAAS VSRPSKTELS TSTLAQIAQF VATTGQTVNI CDVHEWVRDH 

       550        560        570        580        590        600 
NQIRAESEID STQAILCMPI VNAQKVVIGV AQLINKANGV PFTESDASIF EAFAIFCGLG 

       610        620        630        640        650        660 
IHNTQMYENA CKLMAKQKVA LECLSYHATA SQDQTEKLTQ DAIAEAESYN LYSFTFTDFE 

       670        680        690        700        710        720 
LVDDDTCRAV LRMFLQCNLV SQFQIPYDVL CRWVLSVRKN YRPVKYHNWR HALNVAQTMF 

       730        740        750        760        770        780 
AMLKTGKMER FMTDLEILGL LVACLCHDLD HRGTNNAFQT KTESPLAILY TTSTMEHHHF 

       790        800        810        820        830        840 
DQCVMILNSE GNNIFQALSP EDYRSVMKTV ESAILSTDLA MYFKKRNAFL ELVENGEFDW 

       850        860        870        880        890        900 
QGEEKKDLLC GMMMTACDVS AIAKPWEVQH KVAKLVADEF FDQGDLEKLQ LNTQPVAMMD 

       910        920        930        940        950        960 
RERKDELPKM QVGFIDVICL PLYRVLCDTF PWITPLYEGT LENRRNWQDL AEKVEMGLTW 

       970        980        990       1000       1010       1020 
IDHDTIDKPV EEFAGCADEE IKDIEFTVTT LNCNQQAQHG AGAGGDDSHT PEHQRSGSRL 

      1030       1040       1050       1060       1070       1080 
SMKKTGALGK AVRSKLSKTL YNSMDGSKPK TSLKLLESHV SEDMDDKSPT SPSQPHAGGS 

      1090       1100       1110 
VGRMSASSST SSAGTVVDKS KKRSKLCSLL 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CM000070 Genomic DNA. Translation: EAL27911.3.
RefSeqXP_001358768.3. XM_001358731.3.

3D structure databases

ProteinModelPortalQ298P4.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING7237.FBpp0282030.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaFBtr0307989; FBpp0300350; FBgn0080939.
GeneID4801723.
KEGGdpo:Dpse_GA20950.

Organism-specific databases

FlyBaseFBgn0080939. Dpse\GA20950.

Phylogenomic databases

eggNOGNOG270709.
InParanoidQ298P4.
KOK13763.
OrthoDBEOG7RRF69.

Family and domain databases

Gene3D1.10.1300.10. 1 hit.
3.30.450.40. 3 hits.
InterProIPR003018. GAF.
IPR029016. GAF_dom_like.
IPR003607. HD/PDEase_dom.
IPR023088. PDEase.
IPR002073. PDEase_catalytic_dom.
IPR023174. PDEase_CS.
[Graphical view]
PfamPF01590. GAF. 2 hits.
PF00233. PDEase_I. 1 hit.
[Graphical view]
PRINTSPR00387. PDIESTERASE1.
SMARTSM00065. GAF. 2 hits.
SM00471. HDc. 1 hit.
[Graphical view]
SUPFAMSSF55781. SSF55781. 3 hits.
PROSITEPS00126. PDEASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePDE6_DROPS
AccessionPrimary (citable) accession number: Q298P4
Entry history
Integrated into UniProtKB/Swiss-Prot: February 10, 2009
Last sequence update: October 3, 2012
Last modified: June 11, 2014
This is version 49 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Relevant documents

SIMILARITY comments

Index of protein domains and families

Drosophila

Drosophila: entries, gene names and cross-references to FlyBase