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Q298P4

- PDE6_DROPS

UniProt

Q298P4 - PDE6_DROPS

Protein

cGMP-specific 3',5'-cyclic phosphodiesterase

Gene

Pde6

Organism
Drosophila pseudoobscura pseudoobscura (Fruit fly)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 50 (01 Oct 2014)
      Sequence version 3 (03 Oct 2012)
      Previous versions | rss
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    Functioni

    Has a role regulating cGMP transport in Malpighian tubule principal cells.By similarity

    Catalytic activityi

    Guanosine 3',5'-cyclic phosphate + H2O = guanosine 5'-phosphate.By similarity

    Cofactori

    Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei707 – 7071Proton donorBy similarity
    Metal bindingi711 – 7111Divalent metal cation 1By similarity
    Metal bindingi747 – 7471Divalent metal cation 1By similarity
    Metal bindingi748 – 7481Divalent metal cation 1By similarity
    Metal bindingi748 – 7481Divalent metal cation 2By similarity
    Metal bindingi858 – 8581Divalent metal cation 1By similarity

    GO - Molecular functioni

    1. 3',5'-cyclic-GMP phosphodiesterase activity Source: UniProtKB
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. cGMP metabolic process Source: UniProtKB
    2. signal transduction Source: InterPro

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    cGMP, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    cGMP-specific 3',5'-cyclic phosphodiesteraseBy similarity (EC:3.1.4.35)
    Gene namesi
    Name:Pde6By similarity
    ORF Names:GA20950
    OrganismiDrosophila pseudoobscura pseudoobscura (Fruit fly)
    Taxonomic identifieri46245 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
    ProteomesiUP000001819: Chromosome 2, UP000001819: Partially assembled WGS sequence

    Organism-specific databases

    FlyBaseiFBgn0080939. Dpse\GA20950.

    Subcellular locationi

    Cell membrane By similarity; Lipid-anchor By similarity; Cytoplasmic side By similarity

    GO - Cellular componenti

    1. membrane Source: UniProtKB
    2. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 11071107cGMP-specific 3',5'-cyclic phosphodiesterasePRO_0000363694Add
    BLAST
    Propeptidei1108 – 11103Removed in mature formBy similarityPRO_0000363695

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1107 – 11071Cysteine methyl esterBy similarity
    Lipidationi1107 – 11071S-farnesyl cysteineBy similarity

    Keywords - PTMi

    Lipoprotein, Methylation, Prenylation

    Interactioni

    Subunit structurei

    Interacts with PrBP.By similarity

    Protein-protein interaction databases

    STRINGi7237.FBpp0282030.

    Structurei

    3D structure databases

    ProteinModelPortaliQ298P4.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini233 – 385153GAF 1Add
    BLAST
    Domaini417 – 601185GAF 2Add
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi6 – 6055Ala-richAdd
    BLAST

    Sequence similaritiesi

    Belongs to the cyclic nucleotide phosphodiesterase family.Sequence Analysis
    Contains 2 GAF domains.Sequence Analysis

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiNOG270709.
    InParanoidiQ298P4.
    KOiK13763.
    OrthoDBiEOG7RRF69.

    Family and domain databases

    Gene3Di1.10.1300.10. 1 hit.
    3.30.450.40. 3 hits.
    InterProiIPR003018. GAF.
    IPR029016. GAF_dom_like.
    IPR003607. HD/PDEase_dom.
    IPR023088. PDEase.
    IPR002073. PDEase_catalytic_dom.
    IPR023174. PDEase_CS.
    [Graphical view]
    PfamiPF01590. GAF. 2 hits.
    PF00233. PDEase_I. 1 hit.
    [Graphical view]
    PRINTSiPR00387. PDIESTERASE1.
    SMARTiSM00065. GAF. 2 hits.
    SM00471. HDc. 1 hit.
    [Graphical view]
    SUPFAMiSSF55781. SSF55781. 3 hits.
    PROSITEiPS00126. PDEASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q298P4-1 [UniParc]FASTAAdd to Basket

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    MTDVSAAAGG ATAPAETAAT SSSASKPLTN GANKTSTAMA APTATPTTAA     50
    TASGAAEAGA ITSVAGISNQ VKLEHHHHRQ SNNNRPVAPY PPVPAAKPKP 100
    TPTPESKFKS TSREPGMSSS SSSAQQDVDE VARLFEEKPE AFEKWLTERA 150
    PPEALSRLQE FIESRKPLKR PSVTSDLFQQ WMSASPTVQQ KSPRSLSNSS 200
    ASSTLPECRR HLMDLDEGEL FMELIRDVAN ELDIDVLCHK ILVNVGLLTH 250
    ADRGSLFLAK GTPHNKYLVA KLFDVTQKTA LKDAVTRASA EEIIIPFGIG 300
    IAGMVAQTKQ MINIKEAYKD ARFNCEIDLK TGYKTNAILC MPICNYEGDI 350
    IGVAQIINKT NGCMEFDEHD VEIFRRYLTF CGIGIQNAQL FEMSVQEYRR 400
    NQILLNLARS IFEEQNNLEC LVTKIMTEAR ELLNCERCSV FLVDLDCCEA 450
    SHLEKIIEKP NQPEQRPTRA IMPGDSFDEK KMRNRFTVLF ELGGEYQAAS 500
    VSRPSKTELS TSTLAQIAQF VATTGQTVNI CDVHEWVRDH NQIRAESEID 550
    STQAILCMPI VNAQKVVIGV AQLINKANGV PFTESDASIF EAFAIFCGLG 600
    IHNTQMYENA CKLMAKQKVA LECLSYHATA SQDQTEKLTQ DAIAEAESYN 650
    LYSFTFTDFE LVDDDTCRAV LRMFLQCNLV SQFQIPYDVL CRWVLSVRKN 700
    YRPVKYHNWR HALNVAQTMF AMLKTGKMER FMTDLEILGL LVACLCHDLD 750
    HRGTNNAFQT KTESPLAILY TTSTMEHHHF DQCVMILNSE GNNIFQALSP 800
    EDYRSVMKTV ESAILSTDLA MYFKKRNAFL ELVENGEFDW QGEEKKDLLC 850
    GMMMTACDVS AIAKPWEVQH KVAKLVADEF FDQGDLEKLQ LNTQPVAMMD 900
    RERKDELPKM QVGFIDVICL PLYRVLCDTF PWITPLYEGT LENRRNWQDL 950
    AEKVEMGLTW IDHDTIDKPV EEFAGCADEE IKDIEFTVTT LNCNQQAQHG 1000
    AGAGGDDSHT PEHQRSGSRL SMKKTGALGK AVRSKLSKTL YNSMDGSKPK 1050
    TSLKLLESHV SEDMDDKSPT SPSQPHAGGS VGRMSASSST SSAGTVVDKS 1100
    KKRSKLCSLL 1110
    Length:1,110
    Mass (Da):123,338
    Last modified:October 3, 2012 - v3
    Checksum:i4FB8DECD51B085A6
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CM000070 Genomic DNA. Translation: EAL27911.3.
    RefSeqiXP_001358768.3. XM_001358731.3.

    Genome annotation databases

    EnsemblMetazoaiFBtr0307989; FBpp0300350; FBgn0080939.
    GeneIDi4801723.
    KEGGidpo:Dpse_GA20950.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CM000070 Genomic DNA. Translation: EAL27911.3 .
    RefSeqi XP_001358768.3. XM_001358731.3.

    3D structure databases

    ProteinModelPortali Q298P4.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 7237.FBpp0282030.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai FBtr0307989 ; FBpp0300350 ; FBgn0080939 .
    GeneIDi 4801723.
    KEGGi dpo:Dpse_GA20950.

    Organism-specific databases

    FlyBasei FBgn0080939. Dpse\GA20950.

    Phylogenomic databases

    eggNOGi NOG270709.
    InParanoidi Q298P4.
    KOi K13763.
    OrthoDBi EOG7RRF69.

    Family and domain databases

    Gene3Di 1.10.1300.10. 1 hit.
    3.30.450.40. 3 hits.
    InterProi IPR003018. GAF.
    IPR029016. GAF_dom_like.
    IPR003607. HD/PDEase_dom.
    IPR023088. PDEase.
    IPR002073. PDEase_catalytic_dom.
    IPR023174. PDEase_CS.
    [Graphical view ]
    Pfami PF01590. GAF. 2 hits.
    PF00233. PDEase_I. 1 hit.
    [Graphical view ]
    PRINTSi PR00387. PDIESTERASE1.
    SMARTi SM00065. GAF. 2 hits.
    SM00471. HDc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF55781. SSF55781. 3 hits.
    PROSITEi PS00126. PDEASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution."
      Richards S., Liu Y., Bettencourt B.R., Hradecky P., Letovsky S., Nielsen R., Thornton K., Hubisz M.J., Chen R., Meisel R.P., Couronne O., Hua S., Smith M.A., Zhang P., Liu J., Bussemaker H.J., van Batenburg M.F., Howells S.L.
      , Scherer S.E., Sodergren E., Matthews B.B., Crosby M.A., Schroeder A.J., Ortiz-Barrientos D., Rives C.M., Metzker M.L., Muzny D.M., Scott G., Steffen D., Wheeler D.A., Worley K.C., Havlak P., Durbin K.J., Egan A., Gill R., Hume J., Morgan M.B., Miner G., Hamilton C., Huang Y., Waldron L., Verduzco D., Clerc-Blankenburg K.P., Dubchak I., Noor M.A.F., Anderson W., White K.P., Clark A.G., Schaeffer S.W., Gelbart W.M., Weinstock G.M., Gibbs R.A.
      Genome Res. 15:1-18(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: MV2-25 / Tucson 14011-0121.94.

    Entry informationi

    Entry nameiPDE6_DROPS
    AccessioniPrimary (citable) accession number: Q298P4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 10, 2009
    Last sequence update: October 3, 2012
    Last modified: October 1, 2014
    This is version 50 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programDrosophila annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Drosophila
      Drosophila: entries, gene names and cross-references to FlyBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3