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Q298P4

- PDE6_DROPS

UniProt

Q298P4 - PDE6_DROPS

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Protein

cGMP-specific 3',5'-cyclic phosphodiesterase

Gene

Pde6

Organism
Drosophila pseudoobscura pseudoobscura (Fruit fly)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Has a role regulating cGMP transport in Malpighian tubule principal cells.By similarity

Catalytic activityi

Guanosine 3',5'-cyclic phosphate + H2O = guanosine 5'-phosphate.By similarity

Cofactori

Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei707 – 7071Proton donorBy similarity
Metal bindingi711 – 7111Divalent metal cation 1By similarity
Metal bindingi747 – 7471Divalent metal cation 1By similarity
Metal bindingi748 – 7481Divalent metal cation 1By similarity
Metal bindingi748 – 7481Divalent metal cation 2By similarity
Metal bindingi858 – 8581Divalent metal cation 1By similarity

GO - Molecular functioni

  1. 3',5'-cyclic-GMP phosphodiesterase activity Source: UniProtKB
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. cGMP metabolic process Source: UniProtKB
  2. signal transduction Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

cGMP, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
cGMP-specific 3',5'-cyclic phosphodiesteraseBy similarity (EC:3.1.4.35)
Gene namesi
Name:Pde6By similarity
ORF Names:GA20950
OrganismiDrosophila pseudoobscura pseudoobscura (Fruit fly)
Taxonomic identifieri46245 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
ProteomesiUP000001819: Chromosome 2, UP000001819: Partially assembled WGS sequence

Organism-specific databases

FlyBaseiFBgn0080939. Dpse\GA20950.

Subcellular locationi

Cell membrane By similarity; Lipid-anchor By similarity; Cytoplasmic side By similarity

GO - Cellular componenti

  1. membrane Source: UniProtKB
  2. plasma membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11071107cGMP-specific 3',5'-cyclic phosphodiesterasePRO_0000363694Add
BLAST
Propeptidei1108 – 11103Removed in mature formBy similarityPRO_0000363695

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1107 – 11071Cysteine methyl esterBy similarity
Lipidationi1107 – 11071S-farnesyl cysteineBy similarity

Keywords - PTMi

Lipoprotein, Methylation, Prenylation

Interactioni

Subunit structurei

Interacts with PrBP.By similarity

Protein-protein interaction databases

STRINGi7237.FBpp0282030.

Structurei

3D structure databases

ProteinModelPortaliQ298P4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini233 – 385153GAF 1Add
BLAST
Domaini417 – 601185GAF 2Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi6 – 6055Ala-richAdd
BLAST

Sequence similaritiesi

Belongs to the cyclic nucleotide phosphodiesterase family.Sequence Analysis
Contains 2 GAF domains.Sequence Analysis

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiNOG270709.
InParanoidiQ298P4.
KOiK13763.
OrthoDBiEOG7RRF69.

Family and domain databases

Gene3Di1.10.1300.10. 1 hit.
3.30.450.40. 3 hits.
InterProiIPR003018. GAF.
IPR029016. GAF_dom_like.
IPR003607. HD/PDEase_dom.
IPR023088. PDEase.
IPR002073. PDEase_catalytic_dom.
IPR023174. PDEase_CS.
[Graphical view]
PfamiPF01590. GAF. 2 hits.
PF00233. PDEase_I. 1 hit.
[Graphical view]
PRINTSiPR00387. PDIESTERASE1.
SMARTiSM00065. GAF. 2 hits.
SM00471. HDc. 1 hit.
[Graphical view]
SUPFAMiSSF55781. SSF55781. 3 hits.
PROSITEiPS00126. PDEASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q298P4-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTDVSAAAGG ATAPAETAAT SSSASKPLTN GANKTSTAMA APTATPTTAA
60 70 80 90 100
TASGAAEAGA ITSVAGISNQ VKLEHHHHRQ SNNNRPVAPY PPVPAAKPKP
110 120 130 140 150
TPTPESKFKS TSREPGMSSS SSSAQQDVDE VARLFEEKPE AFEKWLTERA
160 170 180 190 200
PPEALSRLQE FIESRKPLKR PSVTSDLFQQ WMSASPTVQQ KSPRSLSNSS
210 220 230 240 250
ASSTLPECRR HLMDLDEGEL FMELIRDVAN ELDIDVLCHK ILVNVGLLTH
260 270 280 290 300
ADRGSLFLAK GTPHNKYLVA KLFDVTQKTA LKDAVTRASA EEIIIPFGIG
310 320 330 340 350
IAGMVAQTKQ MINIKEAYKD ARFNCEIDLK TGYKTNAILC MPICNYEGDI
360 370 380 390 400
IGVAQIINKT NGCMEFDEHD VEIFRRYLTF CGIGIQNAQL FEMSVQEYRR
410 420 430 440 450
NQILLNLARS IFEEQNNLEC LVTKIMTEAR ELLNCERCSV FLVDLDCCEA
460 470 480 490 500
SHLEKIIEKP NQPEQRPTRA IMPGDSFDEK KMRNRFTVLF ELGGEYQAAS
510 520 530 540 550
VSRPSKTELS TSTLAQIAQF VATTGQTVNI CDVHEWVRDH NQIRAESEID
560 570 580 590 600
STQAILCMPI VNAQKVVIGV AQLINKANGV PFTESDASIF EAFAIFCGLG
610 620 630 640 650
IHNTQMYENA CKLMAKQKVA LECLSYHATA SQDQTEKLTQ DAIAEAESYN
660 670 680 690 700
LYSFTFTDFE LVDDDTCRAV LRMFLQCNLV SQFQIPYDVL CRWVLSVRKN
710 720 730 740 750
YRPVKYHNWR HALNVAQTMF AMLKTGKMER FMTDLEILGL LVACLCHDLD
760 770 780 790 800
HRGTNNAFQT KTESPLAILY TTSTMEHHHF DQCVMILNSE GNNIFQALSP
810 820 830 840 850
EDYRSVMKTV ESAILSTDLA MYFKKRNAFL ELVENGEFDW QGEEKKDLLC
860 870 880 890 900
GMMMTACDVS AIAKPWEVQH KVAKLVADEF FDQGDLEKLQ LNTQPVAMMD
910 920 930 940 950
RERKDELPKM QVGFIDVICL PLYRVLCDTF PWITPLYEGT LENRRNWQDL
960 970 980 990 1000
AEKVEMGLTW IDHDTIDKPV EEFAGCADEE IKDIEFTVTT LNCNQQAQHG
1010 1020 1030 1040 1050
AGAGGDDSHT PEHQRSGSRL SMKKTGALGK AVRSKLSKTL YNSMDGSKPK
1060 1070 1080 1090 1100
TSLKLLESHV SEDMDDKSPT SPSQPHAGGS VGRMSASSST SSAGTVVDKS
1110
KKRSKLCSLL
Length:1,110
Mass (Da):123,338
Last modified:October 3, 2012 - v3
Checksum:i4FB8DECD51B085A6
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CM000070 Genomic DNA. Translation: EAL27911.3.
RefSeqiXP_001358768.3. XM_001358731.3.

Genome annotation databases

EnsemblMetazoaiFBtr0307989; FBpp0300350; FBgn0080939.
GeneIDi4801723.
KEGGidpo:Dpse_GA20950.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CM000070 Genomic DNA. Translation: EAL27911.3 .
RefSeqi XP_001358768.3. XM_001358731.3.

3D structure databases

ProteinModelPortali Q298P4.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 7237.FBpp0282030.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai FBtr0307989 ; FBpp0300350 ; FBgn0080939 .
GeneIDi 4801723.
KEGGi dpo:Dpse_GA20950.

Organism-specific databases

FlyBasei FBgn0080939. Dpse\GA20950.

Phylogenomic databases

eggNOGi NOG270709.
InParanoidi Q298P4.
KOi K13763.
OrthoDBi EOG7RRF69.

Family and domain databases

Gene3Di 1.10.1300.10. 1 hit.
3.30.450.40. 3 hits.
InterProi IPR003018. GAF.
IPR029016. GAF_dom_like.
IPR003607. HD/PDEase_dom.
IPR023088. PDEase.
IPR002073. PDEase_catalytic_dom.
IPR023174. PDEase_CS.
[Graphical view ]
Pfami PF01590. GAF. 2 hits.
PF00233. PDEase_I. 1 hit.
[Graphical view ]
PRINTSi PR00387. PDIESTERASE1.
SMARTi SM00065. GAF. 2 hits.
SM00471. HDc. 1 hit.
[Graphical view ]
SUPFAMi SSF55781. SSF55781. 3 hits.
PROSITEi PS00126. PDEASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution."
    Richards S., Liu Y., Bettencourt B.R., Hradecky P., Letovsky S., Nielsen R., Thornton K., Hubisz M.J., Chen R., Meisel R.P., Couronne O., Hua S., Smith M.A., Zhang P., Liu J., Bussemaker H.J., van Batenburg M.F., Howells S.L.
    , Scherer S.E., Sodergren E., Matthews B.B., Crosby M.A., Schroeder A.J., Ortiz-Barrientos D., Rives C.M., Metzker M.L., Muzny D.M., Scott G., Steffen D., Wheeler D.A., Worley K.C., Havlak P., Durbin K.J., Egan A., Gill R., Hume J., Morgan M.B., Miner G., Hamilton C., Huang Y., Waldron L., Verduzco D., Clerc-Blankenburg K.P., Dubchak I., Noor M.A.F., Anderson W., White K.P., Clark A.G., Schaeffer S.W., Gelbart W.M., Weinstock G.M., Gibbs R.A.
    Genome Res. 15:1-18(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MV2-25 / Tucson 14011-0121.94.

Entry informationi

Entry nameiPDE6_DROPS
AccessioniPrimary (citable) accession number: Q298P4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 10, 2009
Last sequence update: October 3, 2012
Last modified: October 29, 2014
This is version 51 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3