Skip Header

Contribute Send feedback
Read comments (?) or add your own

Q298L4 (SPAST_DROPS) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 44. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Spastin
EC=3.6.4.3
Gene names
Name:spas
ORF Names:GA19274
OrganismDrosophila pseudoobscura pseudoobscura (Fruit fly)
Taxonomic identifier46245 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length788 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

ATP-dependent microtubule severing protein. Stimulates microtubule minus-end depolymerization and poleward microtubule flux in the mitotic spindle. Regulates microtubule stability in the neuromuscular junction synapse By similarity.

Catalytic activity

ATP + H2O = ADP + phosphate.

Subunit structure

Homohexamer. The homohexamer is stabilized by ATP-binding. The homohexamer may adopt a ring conformation through which microtubules pass prior to being severed. Interacts with microtubules By similarity.

Subcellular location

Membrane; Single-pass membrane protein Potential. Cytoplasm › cytoskeleton › centrosome By similarity. Cytoplasm › cytoskeleton By similarity. Chromosome By similarity. Note: Colocalizes with cellular microtubule arrays. Localizes to chromosomes from prometaphase/metaphase to anaphase, and this requires microtubules. Localizes to discrete punctate cytoplasmic foci which may correspond to secretory vesicles By similarity.

Sequence similarities

Belongs to the AAA ATPase family. Spastin subfamily.

Contains 1 MIT domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 788788Spastin
PRO_0000367147

Regions

Transmembrane110 – 12920Helical; Potential
Domain239 – 31577MIT
Nucleotide binding553 – 5608ATP Potential
Region1 – 217217Required for localization to punctate cytoplasmic foci By similarity
Region214 – 788575Sufficient for interaction with microtubules and microtubule severing By similarity
Region471 – 48515Required for interaction with microtubules By similarity

Amino acid modifications

Modified residue4671Phosphothreonine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q298L4 [UniParc].

Last modified April 4, 2006. Version 1.
Checksum: E18E25624D5FD127

FASTA78886,084
        10         20         30         40         50         60 
MVRTKNQSSS SSASSSSHKS PIKSHGGSGS AAAGTAGHPV SRSSSSHRTS IDDRKSATNV 

        70         80         90        100        110        120 
SSSSNRRTTP GSSPDGDGDD DTTTTDDLTP TSTSAPRSAG GPSSVHKQNL YVVSFPIIFL 

       130        140        150        160        170        180 
FNVLRSLIYQ LFCIFRYLYG ASTKVIYRPH RRDCNIEIVV QNNSNNKDQK HQQLTSSQSL 

       190        200        210        220        230        240 
NYPLEVTSGE AASEQQVQQP LPQQRYRALQ PLEMAGANRS GSGYSPGPGD PLLAKQKHHH 

       250        260        270        280        290        300 
RRAFEYISKA LKIDEENEGH KELAIELYRK GIKELEDGIA VDCWSGRGDV WDRAQRLHDK 

       310        320        330        340        350        360 
MQTNLSMARD RLHFLALREE DFQMHRLSLK EEQKPNPSRE QHQKPQKARE AADKPMLTNL 

       370        380        390        400        410        420 
TNDPVKLKTR SSGYGPKNGL TTPRISATAT TPTSSSSLAS GRKLTIGTKR PGNLAVAANK 

       430        440        450        460        470        480 
SQTLPRNLGS KTSVGAVRQP GKTAATPPAV RRQFSSGRNT PPQRSRTPIN NNGASGSGSG 

       490        500        510        520        530        540 
ASTPVVTVKG VEQKLVQLIL DEIVEGGAKV EWTDIAGQEV AKQALQEMVI LPSVRPELFT 

       550        560        570        580        590        600 
GLRAPAKGLL LFGPPGNGKT LLARAVATEC SATFLNISAA SLTSKYVGDG EKLVRALFAV 

       610        620        630        640        650        660 
ARHLQPSIIF IDEVDSLLSE RSSGEHEASR RLKTEFLVEF DGLPGNPDGD RIVVLAATNR 

       670        680        690        700        710        720 
PQELDEAALR RFTKRVYVSL PDEQTRELLL NRLLQKQGSP LDTDALRRLS KITDGYSGSD 

       730        740        750        760        770        780 
LTALAKDAAL EPIRELNVEQ VKCLDINAMR HITEKDFHNS LKRIRRSVAQ QSLSSYEKWS 


SDYGDITI

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CM000070 Genomic DNA. Translation: EAL27941.1.
RefSeqXP_001358798.1. XM_001358761.1.

3D structure databases

ProteinModelPortalQ298L4.
SMRQ298L4. Positions 494-780.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID4801757.
GenomeReviewsGene locus spas in contig CM000070_GR.
KEGGdpo:Dpse_GA19274.

Organism-specific databases

FlyBaseFBgn0079271. Dpse\GA19274.

Phylogenomic databases

InParanoidQ298L4.
OMAFLNISAA.
OrthoDBEOG44XGXW.
PhylomeDBQ298L4.

Family and domain databases

InterProIPR003593. ATPase_AAA+_core.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR007330. MIT.
[Graphical view]
KOK13254.
PfamPF00004. AAA. 1 hit.
PF04212. MIT. 1 hit.
[Graphical view]
SMARTSM00382. AAA. 1 hit.
SM00745. MIT. 1 hit.
[Graphical view]
PROSITEPS00674. AAA. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSPAST_DROPS
AccessionPrimary (citable) accession number: Q298L4
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: April 4, 2006
Last modified: November 16, 2011
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Relevant documents

Drosophila

Drosophila: entries, gene names and cross-references to FlyBase

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents