Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q297F5

- PNPH_DROPS

UniProt

Q297F5 - PNPH_DROPS

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Purine nucleoside phosphorylase

Gene

GA16019

Organism
Drosophila pseudoobscura pseudoobscura (Fruit fly)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Purine nucleoside phosphorylase involved in purine salvage.UniRule annotation

Catalytic activityi

Purine nucleoside + phosphate = purine + alpha-D-ribose 1-phosphate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei183 – 1831Important for substrate specificityUniRule annotation
Binding sitei201 – 2011Substrate; via amide nitrogenUniRule annotation
Binding sitei202 – 2021PhosphateUniRule annotation
Sitei238 – 2381Important for substrate specificityUniRule annotation

GO - Molecular functioni

  1. phosphorylase activity Source: InterPro
  2. purine-nucleoside phosphorylase activity Source: UniProtKB-EC
  3. S-methyl-5-thioadenosine phosphorylase activity Source: InterPro

GO - Biological processi

  1. purine ribonucleoside salvage Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Purine salvage

Enzyme and pathway databases

UniPathwayiUPA00606.

Names & Taxonomyi

Protein namesi
Recommended name:
Purine nucleoside phosphorylaseUniRule annotation (EC:2.4.2.1UniRule annotation)
Short name:
PNPUniRule annotation
Gene namesi
ORF Names:GA16019
OrganismiDrosophila pseudoobscura pseudoobscura (Fruit fly)
Taxonomic identifieri46245 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
ProteomesiUP000001819: Chromosome 2, UP000001819: Partially assembled WGS sequence

Organism-specific databases

FlyBaseiFBgn0076035. Dpse\GA16019.

Subcellular locationi

Cytoplasm UniRule annotation. Nucleus UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
  2. nucleus Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 288288Purine nucleoside phosphorylasePRO_0000415089Add
BLAST

Interactioni

Subunit structurei

Homotrimer.UniRule annotation

Protein-protein interaction databases

STRINGi7237.FBpp0281762.

Structurei

3D structure databases

ProteinModelPortaliQ297F5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni65 – 662Phosphate bindingUniRule annotation

Sequence similaritiesi

Belongs to the PNP/MTAP phosphorylase family. MTAP subfamily.UniRule annotation

Phylogenomic databases

InParanoidiQ297F5.
OrthoDBiEOG771270.

Family and domain databases

Gene3Di3.40.50.1580. 1 hit.
HAMAPiMF_01963. MTAP.
InterProiIPR010044. MTAP.
IPR000845. Nucleoside_phosphorylase_d.
IPR001369. PNP/MTAP.
[Graphical view]
PANTHERiPTHR11904. PTHR11904. 1 hit.
PfamiPF01048. PNP_UDP_1. 1 hit.
[Graphical view]
SUPFAMiSSF53167. SSF53167. 1 hit.

Sequencei

Sequence statusi: Complete.

Q297F5-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MENNLAPINE DPFPIKIGII GDADLDTTIS LQDRMEYAVC TPFGKPSDII
60 70 80 90 100
IEGLIDGVKC ALLCRNGRLH DIMPTNINYR ANIWAMRKLG CTHILVTHSL
110 120 130 140 150
SSLREDIMPG DFVVPNDLID HTTRRAQTFY DGALGSPFGV CHLPMYPAFC
160 170 180 190 200
ERTRQHLLNA AQELDLATHS KATVLTLEGP RYSTLAENNI YRKWGADLLS
210 220 230 240 250
MTLSPEATLA KEAGILYASI GLVTNIECWC ANQPIATTHE IIYVFKNKVE
260 270 280
KLQQVLSKAI ANISKEDWSE DILKAKILVC SNFANRNK
Length:288
Mass (Da):32,088
Last modified:October 14, 2008 - v2
Checksum:iBF5C8F6883BC1B7A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CM000070 Genomic DNA. Translation: EAL28250.2.
RefSeqiXP_001359107.2. XM_001359070.2.

Genome annotation databases

EnsemblMetazoaiFBtr0283324; FBpp0281762; FBgn0076035.
GeneIDi4802126.
KEGGidpo:Dpse_GA16019.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CM000070 Genomic DNA. Translation: EAL28250.2 .
RefSeqi XP_001359107.2. XM_001359070.2.

3D structure databases

ProteinModelPortali Q297F5.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 7237.FBpp0281762.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai FBtr0283324 ; FBpp0281762 ; FBgn0076035 .
GeneIDi 4802126.
KEGGi dpo:Dpse_GA16019.

Organism-specific databases

FlyBasei FBgn0076035. Dpse\GA16019.

Phylogenomic databases

InParanoidi Q297F5.
OrthoDBi EOG771270.

Enzyme and pathway databases

UniPathwayi UPA00606 .

Family and domain databases

Gene3Di 3.40.50.1580. 1 hit.
HAMAPi MF_01963. MTAP.
InterProi IPR010044. MTAP.
IPR000845. Nucleoside_phosphorylase_d.
IPR001369. PNP/MTAP.
[Graphical view ]
PANTHERi PTHR11904. PTHR11904. 1 hit.
Pfami PF01048. PNP_UDP_1. 1 hit.
[Graphical view ]
SUPFAMi SSF53167. SSF53167. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution."
    Richards S., Liu Y., Bettencourt B.R., Hradecky P., Letovsky S., Nielsen R., Thornton K., Hubisz M.J., Chen R., Meisel R.P., Couronne O., Hua S., Smith M.A., Zhang P., Liu J., Bussemaker H.J., van Batenburg M.F., Howells S.L.
    , Scherer S.E., Sodergren E., Matthews B.B., Crosby M.A., Schroeder A.J., Ortiz-Barrientos D., Rives C.M., Metzker M.L., Muzny D.M., Scott G., Steffen D., Wheeler D.A., Worley K.C., Havlak P., Durbin K.J., Egan A., Gill R., Hume J., Morgan M.B., Miner G., Hamilton C., Huang Y., Waldron L., Verduzco D., Clerc-Blankenburg K.P., Dubchak I., Noor M.A.F., Anderson W., White K.P., Clark A.G., Schaeffer S.W., Gelbart W.M., Weinstock G.M., Gibbs R.A.
    Genome Res. 15:1-18(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MV2-25 / Tucson 14011-0121.94.

Entry informationi

Entry nameiPNPH_DROPS
AccessioniPrimary (citable) accession number: Q297F5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 25, 2012
Last sequence update: October 14, 2008
Last modified: October 29, 2014
This is version 47 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Miscellaneous

Although this enzyme belongs to the family of MTA phosphorylases based on sequence homology, it lacks several conserved amino acids in the substrate binding pocket that confer specificity towards MTA.UniRule annotation

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3