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Protein

Purine nucleoside phosphorylase

Gene

GA16019

Organism
Drosophila pseudoobscura pseudoobscura (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Purine nucleoside phosphorylase involved in purine salvage.UniRule annotation

Catalytic activityi

Purine nucleoside + phosphate = purine + alpha-D-ribose 1-phosphate.UniRule annotation

Pathway:ipurine nucleoside salvage

This protein is involved in the pathway purine nucleoside salvage, which is part of Purine metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway purine nucleoside salvage and in Purine metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei183 – 1831Important for substrate specificityUniRule annotation
Binding sitei201 – 2011Substrate; via amide nitrogenUniRule annotation
Binding sitei202 – 2021PhosphateUniRule annotation
Sitei238 – 2381Important for substrate specificityUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Purine salvage

Enzyme and pathway databases

UniPathwayiUPA00606.

Names & Taxonomyi

Protein namesi
Recommended name:
Purine nucleoside phosphorylaseUniRule annotation (EC:2.4.2.1UniRule annotation)
Short name:
PNPUniRule annotation
Gene namesi
ORF Names:GA16019
OrganismiDrosophila pseudoobscura pseudoobscura (Fruit fly)
Taxonomic identifieri46245 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
ProteomesiUP000001819 Componentsi: Chromosome 2, Partially assembled WGS sequence

Organism-specific databases

FlyBaseiFBgn0076035. Dpse\GA16019.

Subcellular locationi

  • Cytoplasm UniRule annotation
  • Nucleus UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 288288Purine nucleoside phosphorylasePRO_0000415089Add
BLAST

Interactioni

Subunit structurei

Homotrimer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ297F5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni65 – 662Phosphate bindingUniRule annotation

Sequence similaritiesi

Belongs to the PNP/MTAP phosphorylase family. MTAP subfamily.UniRule annotation

Phylogenomic databases

InParanoidiQ297F5.
OMAiMEGPRFE.
OrthoDBiEOG771270.

Family and domain databases

Gene3Di3.40.50.1580. 1 hit.
HAMAPiMF_01963. MTAP.
InterProiIPR010044. MTAP.
IPR000845. Nucleoside_phosphorylase_d.
IPR001369. PNP/MTAP.
[Graphical view]
PANTHERiPTHR11904. PTHR11904. 1 hit.
PfamiPF01048. PNP_UDP_1. 1 hit.
[Graphical view]
SUPFAMiSSF53167. SSF53167. 1 hit.

Sequencei

Sequence statusi: Complete.

Q297F5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MENNLAPINE DPFPIKIGII GDADLDTTIS LQDRMEYAVC TPFGKPSDII
60 70 80 90 100
IEGLIDGVKC ALLCRNGRLH DIMPTNINYR ANIWAMRKLG CTHILVTHSL
110 120 130 140 150
SSLREDIMPG DFVVPNDLID HTTRRAQTFY DGALGSPFGV CHLPMYPAFC
160 170 180 190 200
ERTRQHLLNA AQELDLATHS KATVLTLEGP RYSTLAENNI YRKWGADLLS
210 220 230 240 250
MTLSPEATLA KEAGILYASI GLVTNIECWC ANQPIATTHE IIYVFKNKVE
260 270 280
KLQQVLSKAI ANISKEDWSE DILKAKILVC SNFANRNK
Length:288
Mass (Da):32,088
Last modified:October 14, 2008 - v2
Checksum:iBF5C8F6883BC1B7A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CM000070 Genomic DNA. Translation: EAL28250.2.
RefSeqiXP_001359107.2. XM_001359070.2.

Genome annotation databases

EnsemblMetazoaiFBtr0283324; FBpp0281762; FBgn0076035.
GeneIDi4802126.
KEGGidpo:Dpse_GA16019.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CM000070 Genomic DNA. Translation: EAL28250.2.
RefSeqiXP_001359107.2. XM_001359070.2.

3D structure databases

ProteinModelPortaliQ297F5.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0283324; FBpp0281762; FBgn0076035.
GeneIDi4802126.
KEGGidpo:Dpse_GA16019.

Organism-specific databases

FlyBaseiFBgn0076035. Dpse\GA16019.

Phylogenomic databases

InParanoidiQ297F5.
OMAiMEGPRFE.
OrthoDBiEOG771270.

Enzyme and pathway databases

UniPathwayiUPA00606.

Family and domain databases

Gene3Di3.40.50.1580. 1 hit.
HAMAPiMF_01963. MTAP.
InterProiIPR010044. MTAP.
IPR000845. Nucleoside_phosphorylase_d.
IPR001369. PNP/MTAP.
[Graphical view]
PANTHERiPTHR11904. PTHR11904. 1 hit.
PfamiPF01048. PNP_UDP_1. 1 hit.
[Graphical view]
SUPFAMiSSF53167. SSF53167. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution."
    Richards S., Liu Y., Bettencourt B.R., Hradecky P., Letovsky S., Nielsen R., Thornton K., Hubisz M.J., Chen R., Meisel R.P., Couronne O., Hua S., Smith M.A., Zhang P., Liu J., Bussemaker H.J., van Batenburg M.F., Howells S.L.
    , Scherer S.E., Sodergren E., Matthews B.B., Crosby M.A., Schroeder A.J., Ortiz-Barrientos D., Rives C.M., Metzker M.L., Muzny D.M., Scott G., Steffen D., Wheeler D.A., Worley K.C., Havlak P., Durbin K.J., Egan A., Gill R., Hume J., Morgan M.B., Miner G., Hamilton C., Huang Y., Waldron L., Verduzco D., Clerc-Blankenburg K.P., Dubchak I., Noor M.A.F., Anderson W., White K.P., Clark A.G., Schaeffer S.W., Gelbart W.M., Weinstock G.M., Gibbs R.A.
    Genome Res. 15:1-18(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MV2-25 / Tucson 14011-0121.94.

Entry informationi

Entry nameiPNPH_DROPS
AccessioniPrimary (citable) accession number: Q297F5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 25, 2012
Last sequence update: October 14, 2008
Last modified: July 22, 2015
This is version 50 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Miscellaneous

Although this enzyme belongs to the family of MTA phosphorylases based on sequence homology, it lacks several conserved amino acids in the substrate binding pocket that confer specificity towards MTA.UniRule annotation

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.