Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q297F5

- PNPH_DROPS

UniProt

Q297F5 - PNPH_DROPS

Protein

Purine nucleoside phosphorylase

Gene

GA16019

Organism
Drosophila pseudoobscura pseudoobscura (Fruit fly)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 46 (01 Oct 2014)
      Sequence version 2 (14 Oct 2008)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Purine nucleoside phosphorylase involved in purine salvage.UniRule annotation

    Catalytic activityi

    Purine nucleoside + phosphate = purine + alpha-D-ribose 1-phosphate.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei183 – 1831Important for substrate specificityUniRule annotation
    Binding sitei201 – 2011Substrate; via amide nitrogenUniRule annotation
    Binding sitei202 – 2021PhosphateUniRule annotation
    Sitei238 – 2381Important for substrate specificityUniRule annotation

    GO - Molecular functioni

    1. phosphorylase activity Source: InterPro
    2. purine-nucleoside phosphorylase activity Source: UniProtKB-EC
    3. S-methyl-5-thioadenosine phosphorylase activity Source: InterPro

    GO - Biological processi

    1. purine ribonucleoside salvage Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Biological processi

    Purine salvage

    Enzyme and pathway databases

    UniPathwayiUPA00606.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Purine nucleoside phosphorylaseUniRule annotation (EC:2.4.2.1UniRule annotation)
    Short name:
    PNPUniRule annotation
    Gene namesi
    ORF Names:GA16019
    OrganismiDrosophila pseudoobscura pseudoobscura (Fruit fly)
    Taxonomic identifieri46245 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
    ProteomesiUP000001819: Chromosome 2, UP000001819: Partially assembled WGS sequence

    Organism-specific databases

    FlyBaseiFBgn0076035. Dpse\GA16019.

    Subcellular locationi

    Cytoplasm UniRule annotation. Nucleus UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 288288Purine nucleoside phosphorylasePRO_0000415089Add
    BLAST

    Interactioni

    Subunit structurei

    Homotrimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi7237.FBpp0281762.

    Structurei

    3D structure databases

    ProteinModelPortaliQ297F5.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni65 – 662Phosphate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the PNP/MTAP phosphorylase family. MTAP subfamily.UniRule annotation

    Phylogenomic databases

    InParanoidiQ297F5.
    OrthoDBiEOG771270.

    Family and domain databases

    Gene3Di3.40.50.1580. 1 hit.
    HAMAPiMF_01963. MTAP.
    InterProiIPR010044. MTAP.
    IPR000845. Nucleoside_phosphorylase_d.
    IPR001369. PNP/MTAP.
    [Graphical view]
    PANTHERiPTHR11904. PTHR11904. 1 hit.
    PfamiPF01048. PNP_UDP_1. 1 hit.
    [Graphical view]
    SUPFAMiSSF53167. SSF53167. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q297F5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MENNLAPINE DPFPIKIGII GDADLDTTIS LQDRMEYAVC TPFGKPSDII    50
    IEGLIDGVKC ALLCRNGRLH DIMPTNINYR ANIWAMRKLG CTHILVTHSL 100
    SSLREDIMPG DFVVPNDLID HTTRRAQTFY DGALGSPFGV CHLPMYPAFC 150
    ERTRQHLLNA AQELDLATHS KATVLTLEGP RYSTLAENNI YRKWGADLLS 200
    MTLSPEATLA KEAGILYASI GLVTNIECWC ANQPIATTHE IIYVFKNKVE 250
    KLQQVLSKAI ANISKEDWSE DILKAKILVC SNFANRNK 288
    Length:288
    Mass (Da):32,088
    Last modified:October 14, 2008 - v2
    Checksum:iBF5C8F6883BC1B7A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CM000070 Genomic DNA. Translation: EAL28250.2.
    RefSeqiXP_001359107.2. XM_001359070.2.

    Genome annotation databases

    EnsemblMetazoaiFBtr0283324; FBpp0281762; FBgn0076035.
    GeneIDi4802126.
    KEGGidpo:Dpse_GA16019.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CM000070 Genomic DNA. Translation: EAL28250.2 .
    RefSeqi XP_001359107.2. XM_001359070.2.

    3D structure databases

    ProteinModelPortali Q297F5.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 7237.FBpp0281762.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai FBtr0283324 ; FBpp0281762 ; FBgn0076035 .
    GeneIDi 4802126.
    KEGGi dpo:Dpse_GA16019.

    Organism-specific databases

    FlyBasei FBgn0076035. Dpse\GA16019.

    Phylogenomic databases

    InParanoidi Q297F5.
    OrthoDBi EOG771270.

    Enzyme and pathway databases

    UniPathwayi UPA00606 .

    Family and domain databases

    Gene3Di 3.40.50.1580. 1 hit.
    HAMAPi MF_01963. MTAP.
    InterProi IPR010044. MTAP.
    IPR000845. Nucleoside_phosphorylase_d.
    IPR001369. PNP/MTAP.
    [Graphical view ]
    PANTHERi PTHR11904. PTHR11904. 1 hit.
    Pfami PF01048. PNP_UDP_1. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53167. SSF53167. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution."
      Richards S., Liu Y., Bettencourt B.R., Hradecky P., Letovsky S., Nielsen R., Thornton K., Hubisz M.J., Chen R., Meisel R.P., Couronne O., Hua S., Smith M.A., Zhang P., Liu J., Bussemaker H.J., van Batenburg M.F., Howells S.L.
      , Scherer S.E., Sodergren E., Matthews B.B., Crosby M.A., Schroeder A.J., Ortiz-Barrientos D., Rives C.M., Metzker M.L., Muzny D.M., Scott G., Steffen D., Wheeler D.A., Worley K.C., Havlak P., Durbin K.J., Egan A., Gill R., Hume J., Morgan M.B., Miner G., Hamilton C., Huang Y., Waldron L., Verduzco D., Clerc-Blankenburg K.P., Dubchak I., Noor M.A.F., Anderson W., White K.P., Clark A.G., Schaeffer S.W., Gelbart W.M., Weinstock G.M., Gibbs R.A.
      Genome Res. 15:1-18(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: MV2-25 / Tucson 14011-0121.94.

    Entry informationi

    Entry nameiPNPH_DROPS
    AccessioniPrimary (citable) accession number: Q297F5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 25, 2012
    Last sequence update: October 14, 2008
    Last modified: October 1, 2014
    This is version 46 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programDrosophila annotation project

    Miscellaneousi

    Miscellaneous

    Although this enzyme belongs to the family of MTA phosphorylases based on sequence homology, it lacks several conserved amino acids in the substrate binding pocket that confer specificity towards MTA.UniRule annotation

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Drosophila
      Drosophila: entries, gene names and cross-references to FlyBase
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3