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Q297F5

- PNPH_DROPS

UniProt

Q297F5 - PNPH_DROPS

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Protein
Purine nucleoside phosphorylase
Gene
GA16019
Organism
Drosophila pseudoobscura pseudoobscura (Fruit fly)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Purine nucleoside phosphorylase involved in purine salvage By similarity.UniRule annotation

Catalytic activityi

Purine nucleoside + phosphate = purine + alpha-D-ribose 1-phosphate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei183 – 1831Important for substrate specificity By similarity
Binding sitei201 – 2011Substrate; via amide nitrogen By similarity
Binding sitei202 – 2021Phosphate By similarity
Sitei238 – 2381Important for substrate specificity By similarity

GO - Molecular functioni

  1. S-methyl-5-thioadenosine phosphorylase activity Source: InterPro
  2. phosphorylase activity Source: InterPro
  3. purine-nucleoside phosphorylase activity Source: UniProtKB-EC
Complete GO annotation...

GO - Biological processi

  1. purine ribonucleoside salvage Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Purine salvage

Enzyme and pathway databases

UniPathwayiUPA00606.

Names & Taxonomyi

Protein namesi
Recommended name:
Purine nucleoside phosphorylase (EC:2.4.2.1)
Short name:
PNP
Gene namesi
ORF Names:GA16019
OrganismiDrosophila pseudoobscura pseudoobscura (Fruit fly)
Taxonomic identifieri46245 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
ProteomesiUP000001819: Chromosome 2, UP000001819: Partially assembled WGS sequence

Organism-specific databases

FlyBaseiFBgn0076035. Dpse\GA16019.

Subcellular locationi

Cytoplasm By similarity. Nucleus By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 288288Purine nucleoside phosphorylaseUniRule annotation
PRO_0000415089Add
BLAST

Interactioni

Subunit structurei

Homotrimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi7237.FBpp0281762.

Structurei

3D structure databases

ProteinModelPortaliQ297F5.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni65 – 662Phosphate binding By similarity

Sequence similaritiesi

Phylogenomic databases

InParanoidiQ297F5.
OrthoDBiEOG771270.

Family and domain databases

Gene3Di3.40.50.1580. 1 hit.
HAMAPiMF_01963. MTAP.
InterProiIPR010044. MTAP.
IPR000845. Nucleoside_phosphorylase_d.
IPR001369. PNP/MTAP.
[Graphical view]
PANTHERiPTHR11904. PTHR11904. 1 hit.
PfamiPF01048. PNP_UDP_1. 1 hit.
[Graphical view]
SUPFAMiSSF53167. SSF53167. 1 hit.

Sequencei

Sequence statusi: Complete.

Q297F5-1 [UniParc]FASTAAdd to Basket

« Hide

MENNLAPINE DPFPIKIGII GDADLDTTIS LQDRMEYAVC TPFGKPSDII    50
IEGLIDGVKC ALLCRNGRLH DIMPTNINYR ANIWAMRKLG CTHILVTHSL 100
SSLREDIMPG DFVVPNDLID HTTRRAQTFY DGALGSPFGV CHLPMYPAFC 150
ERTRQHLLNA AQELDLATHS KATVLTLEGP RYSTLAENNI YRKWGADLLS 200
MTLSPEATLA KEAGILYASI GLVTNIECWC ANQPIATTHE IIYVFKNKVE 250
KLQQVLSKAI ANISKEDWSE DILKAKILVC SNFANRNK 288
Length:288
Mass (Da):32,088
Last modified:October 14, 2008 - v2
Checksum:iBF5C8F6883BC1B7A
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CM000070 Genomic DNA. Translation: EAL28250.2.
RefSeqiXP_001359107.2. XM_001359070.2.

Genome annotation databases

EnsemblMetazoaiFBtr0283324; FBpp0281762; FBgn0076035.
GeneIDi4802126.
KEGGidpo:Dpse_GA16019.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CM000070 Genomic DNA. Translation: EAL28250.2 .
RefSeqi XP_001359107.2. XM_001359070.2.

3D structure databases

ProteinModelPortali Q297F5.
ModBasei Search...

Protein-protein interaction databases

STRINGi 7237.FBpp0281762.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai FBtr0283324 ; FBpp0281762 ; FBgn0076035 .
GeneIDi 4802126.
KEGGi dpo:Dpse_GA16019.

Organism-specific databases

FlyBasei FBgn0076035. Dpse\GA16019.

Phylogenomic databases

InParanoidi Q297F5.
OrthoDBi EOG771270.

Enzyme and pathway databases

UniPathwayi UPA00606 .

Family and domain databases

Gene3Di 3.40.50.1580. 1 hit.
HAMAPi MF_01963. MTAP.
InterProi IPR010044. MTAP.
IPR000845. Nucleoside_phosphorylase_d.
IPR001369. PNP/MTAP.
[Graphical view ]
PANTHERi PTHR11904. PTHR11904. 1 hit.
Pfami PF01048. PNP_UDP_1. 1 hit.
[Graphical view ]
SUPFAMi SSF53167. SSF53167. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution."
    Richards S., Liu Y., Bettencourt B.R., Hradecky P., Letovsky S., Nielsen R., Thornton K., Hubisz M.J., Chen R., Meisel R.P., Couronne O., Hua S., Smith M.A., Zhang P., Liu J., Bussemaker H.J., van Batenburg M.F., Howells S.L.
    , Scherer S.E., Sodergren E., Matthews B.B., Crosby M.A., Schroeder A.J., Ortiz-Barrientos D., Rives C.M., Metzker M.L., Muzny D.M., Scott G., Steffen D., Wheeler D.A., Worley K.C., Havlak P., Durbin K.J., Egan A., Gill R., Hume J., Morgan M.B., Miner G., Hamilton C., Huang Y., Waldron L., Verduzco D., Clerc-Blankenburg K.P., Dubchak I., Noor M.A.F., Anderson W., White K.P., Clark A.G., Schaeffer S.W., Gelbart W.M., Weinstock G.M., Gibbs R.A.
    Genome Res. 15:1-18(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MV2-25 / Tucson 14011-0121.94.

Entry informationi

Entry nameiPNPH_DROPS
AccessioniPrimary (citable) accession number: Q297F5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 25, 2012
Last sequence update: October 14, 2008
Last modified: June 11, 2014
This is version 45 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Miscellaneous

Although this enzyme belongs to the family of MTA phosphorylases based on sequence homology, it lacks several conserved amino acids in the substrate binding pocket that confer specificity towards MTA By similarity.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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