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Q297F5 (PNPH_DROPS) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 45. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Purine nucleoside phosphorylase

Short name=PNP
EC=2.4.2.1
Gene names
ORF Names:GA16019
OrganismDrosophila pseudoobscura pseudoobscura (Fruit fly) [Reference proteome]
Taxonomic identifier46245 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora

Protein attributes

Sequence length288 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Purine nucleoside phosphorylase involved in purine salvage By similarity. HAMAP-Rule MF_03155

Catalytic activity

Purine nucleoside + phosphate = purine + alpha-D-ribose 1-phosphate. HAMAP-Rule MF_03155

Pathway

Purine metabolism; purine nucleoside salvage. HAMAP-Rule MF_03155

Subunit structure

Homotrimer By similarity. HAMAP-Rule MF_03155

Subcellular location

Cytoplasm By similarity. Nucleus By similarity HAMAP-Rule MF_03155.

Miscellaneous

Although this enzyme belongs to the family of MTA phosphorylases based on sequence homology, it lacks several conserved amino acids in the substrate binding pocket that confer specificity towards MTA By similarity.

Sequence similarities

Belongs to the PNP/MTAP phosphorylase family. MTAP subfamily.

Ontologies

Keywords
   Biological processPurine salvage
   Cellular componentCytoplasm
Nucleus
   Molecular functionGlycosyltransferase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processpurine ribonucleoside salvage

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

nucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionS-methyl-5-thioadenosine phosphorylase activity

Inferred from electronic annotation. Source: InterPro

phosphorylase activity

Inferred from electronic annotation. Source: InterPro

purine-nucleoside phosphorylase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 288288Purine nucleoside phosphorylase HAMAP-Rule MF_03155
PRO_0000415089

Regions

Region65 – 662Phosphate binding By similarity

Sites

Binding site2011Substrate; via amide nitrogen By similarity
Binding site2021Phosphate By similarity
Site1831Important for substrate specificity By similarity
Site2381Important for substrate specificity By similarity

Sequences

Sequence LengthMass (Da)Tools
Q297F5 [UniParc].

Last modified October 14, 2008. Version 2.
Checksum: BF5C8F6883BC1B7A

FASTA28832,088
        10         20         30         40         50         60 
MENNLAPINE DPFPIKIGII GDADLDTTIS LQDRMEYAVC TPFGKPSDII IEGLIDGVKC 

        70         80         90        100        110        120 
ALLCRNGRLH DIMPTNINYR ANIWAMRKLG CTHILVTHSL SSLREDIMPG DFVVPNDLID 

       130        140        150        160        170        180 
HTTRRAQTFY DGALGSPFGV CHLPMYPAFC ERTRQHLLNA AQELDLATHS KATVLTLEGP 

       190        200        210        220        230        240 
RYSTLAENNI YRKWGADLLS MTLSPEATLA KEAGILYASI GLVTNIECWC ANQPIATTHE 

       250        260        270        280 
IIYVFKNKVE KLQQVLSKAI ANISKEDWSE DILKAKILVC SNFANRNK 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CM000070 Genomic DNA. Translation: EAL28250.2.
RefSeqXP_001359107.2. XM_001359070.2.

3D structure databases

ProteinModelPortalQ297F5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING7237.FBpp0281762.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaFBtr0283324; FBpp0281762; FBgn0076035.
GeneID4802126.
KEGGdpo:Dpse_GA16019.

Organism-specific databases

FlyBaseFBgn0076035. Dpse\GA16019.

Phylogenomic databases

InParanoidQ297F5.
OrthoDBEOG771270.

Enzyme and pathway databases

UniPathwayUPA00606.

Family and domain databases

Gene3D3.40.50.1580. 1 hit.
HAMAPMF_01963. MTAP.
InterProIPR010044. MTAP.
IPR000845. Nucleoside_phosphorylase_d.
IPR001369. PNP/MTAP.
[Graphical view]
PANTHERPTHR11904. PTHR11904. 1 hit.
PfamPF01048. PNP_UDP_1. 1 hit.
[Graphical view]
SUPFAMSSF53167. SSF53167. 1 hit.
ProtoNetSearch...

Entry information

Entry namePNPH_DROPS
AccessionPrimary (citable) accession number: Q297F5
Entry history
Integrated into UniProtKB/Swiss-Prot: January 25, 2012
Last sequence update: October 14, 2008
Last modified: June 11, 2014
This is version 45 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Drosophila

Drosophila: entries, gene names and cross-references to FlyBase