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Protein

Prostacyclin synthase

Gene

PTGIS

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the isomerization of prostaglandin H2 to prostacyclin (= prostaglandin I2).

Catalytic activityi

(5Z,13E)-(15S)-9-alpha,11-alpha-epidioxy-15-hydroxyprosta-5,13-dienoate = (5Z,13E)-(15S)-6,9-alpha-epoxy-11-alpha,15-dihydroxyprosta-5,13-dienoate.

Cofactori

hemeBy similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi441 – 4411Iron (heme axial ligand)By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism, Prostaglandin biosynthesis, Prostaglandin metabolism

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

ReactomeiR-BTA-197264. Nicotinamide salvaging.
R-BTA-211979. Eicosanoids.
R-BTA-2162123. Synthesis of Prostaglandins (PG) and Thromboxanes (TX).

Names & Taxonomyi

Protein namesi
Recommended name:
Prostacyclin synthase (EC:5.3.99.4)
Alternative name(s):
Prostaglandin I2 synthase
Gene namesi
Name:PTGIS
Synonyms:CYP8
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 13

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei1 – 2020HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 500500Prostacyclin synthasePRO_0000051909Add
BLAST

Proteomic databases

PaxDbiQ29626.
PRIDEiQ29626.

Interactioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000023313.

Structurei

3D structure databases

ProteinModelPortaliQ29626.
SMRiQ29626. Positions 23-500.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0684. Eukaryota.
COG2124. LUCA.
GeneTreeiENSGT00550000074551.
HOGENOMiHOG000231026.
HOVERGENiHBG051100.
InParanoidiQ29626.
KOiK01831.
OMAiMCSVKSR.
OrthoDBiEOG7J9VP6.
TreeFamiTF105090.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR024204. Cyt_P450_CYP7A1-type.
IPR002403. Cyt_P450_E_grp-IV.
IPR027286. PTGIS.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PIRSFiPIRSF000047. Cytochrome_CYPVIIA1. 1 hit.
PIRSF500628. PTGIS. 1 hit.
PRINTSiPR00465. EP450IV.
SUPFAMiSSF48264. SSF48264. 1 hit.

Sequencei

Sequence statusi: Complete.

Q29626-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSWAVVFGLL AALLLLLLLT RRRTRRPGEP PLDLGSIPWL GHALEFGKDA
60 70 80 90 100
AGFLTRMKEK HGDIFTVLVG GRHVTVLLDP HSYDAVVWEP RSRLDFHAYA
110 120 130 140 150
VFLMERIFDV QLPHYNPGDE KSKMKPTLLH KELQALTDAM YTNLRTVLLG
160 170 180 190 200
DTVEAGSGWH EMGLLEFSYG FLLRAGYLTQ YGVEAPPHTQ ESQAQDRVHS
210 220 230 240 250
ADVFHTFRQL DLLLPKLARG SLSAGDKDRV GKVKGRLWKL LSPTRLASRA
260 270 280 290 300
HRSRWLESYL LHLEEMGVSE EMQARALVLQ LWATQGNMGP AAFWLLLFLL
310 320 330 340 350
KNPEALAAVR GELETVLLGA EQPISQMTTL PQKVLDSMPV LDSVLSESLR
360 370 380 390 400
LTAAPFITRE VVADLALPMA DGREFSLRRG DRLLLFPFLS PQKDPEIYTD
410 420 430 440 450
PEVFKYNRFL NPDGSEKKDF YKDGKRLKNY SLPWGAGHNQ CLGKGYAVNS
460 470 480 490 500
IKQFVFLVLT QFDLELITPD VDIPEFDLSR YGFGLMQPEH DVPVRYRIRP
Length:500
Mass (Da):56,629
Last modified:November 1, 1996 - v1
Checksum:i3728EEFEAE852403
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti192 – 1921S → N in AAA53674 (PubMed:8074709).Curated
Sequence conflicti353 – 3531A → P in AAA53674 (PubMed:8074709).Curated
Sequence conflicti407 – 4071N → T in AAA53674 (PubMed:8074709).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D30718 mRNA. Translation: BAA06383.1.
L34208 mRNA. Translation: AAA53674.1.
BC120087 mRNA. Translation: AAI20088.1.
S67757 mRNA. Translation: AAB29680.1.
PIRiA53658.
RefSeqiNP_776869.1. NM_174444.1.
UniGeneiBt.5247.

Genome annotation databases

EnsembliENSBTAT00000023313; ENSBTAP00000023313; ENSBTAG00000017537.
GeneIDi282021.
KEGGibta:282021.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D30718 mRNA. Translation: BAA06383.1.
L34208 mRNA. Translation: AAA53674.1.
BC120087 mRNA. Translation: AAI20088.1.
S67757 mRNA. Translation: AAB29680.1.
PIRiA53658.
RefSeqiNP_776869.1. NM_174444.1.
UniGeneiBt.5247.

3D structure databases

ProteinModelPortaliQ29626.
SMRiQ29626. Positions 23-500.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000023313.

Proteomic databases

PaxDbiQ29626.
PRIDEiQ29626.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000023313; ENSBTAP00000023313; ENSBTAG00000017537.
GeneIDi282021.
KEGGibta:282021.

Organism-specific databases

CTDi5740.

Phylogenomic databases

eggNOGiKOG0684. Eukaryota.
COG2124. LUCA.
GeneTreeiENSGT00550000074551.
HOGENOMiHOG000231026.
HOVERGENiHBG051100.
InParanoidiQ29626.
KOiK01831.
OMAiMCSVKSR.
OrthoDBiEOG7J9VP6.
TreeFamiTF105090.

Enzyme and pathway databases

ReactomeiR-BTA-197264. Nicotinamide salvaging.
R-BTA-211979. Eicosanoids.
R-BTA-2162123. Synthesis of Prostaglandins (PG) and Thromboxanes (TX).

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR024204. Cyt_P450_CYP7A1-type.
IPR002403. Cyt_P450_E_grp-IV.
IPR027286. PTGIS.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PIRSFiPIRSF000047. Cytochrome_CYPVIIA1. 1 hit.
PIRSF500628. PTGIS. 1 hit.
PRINTSiPR00465. EP450IV.
SUPFAMiSSF48264. SSF48264. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation and molecular cloning of prostacyclin synthase from bovine endothelial cells."
    Hara S., Miyata A., Yokoyama C., Inoue H., Brugger R., Lottspeich F., Ullrich V., Tanabe T.
    J. Biol. Chem. 269:19897-19903(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
    Tissue: Aorta.
  2. "Molecular cloning and characterization of bovine prostacyclin synthase."
    Pereira B., Wu K.K., Wang L.H.
    Biochem. Biophys. Res. Commun. 203:59-66(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
    Tissue: Aorta.
  3. NIH - Mammalian Gene Collection (MGC) project
    Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Fetal cerebellum.
  4. "Bovine prostacyclin synthase: purification and isolation of partial cDNA."
    Pereira B., Wu K.K., Wang L.H.
    Biochem. Biophys. Res. Commun. 197:1041-1048(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 9-30.
    Tissue: Aorta.

Entry informationi

Entry nameiPTGIS_BOVIN
AccessioniPrimary (citable) accession number: Q29626
Secondary accession number(s): Q0P5G0, Q28841
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: June 8, 2016
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.