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Q29577 (KCRU_PIG) Reviewed, UniProtKB/Swiss-Prot

Last modified November 13, 2013. Version 67. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Creatine kinase U-type, mitochondrial

EC=2.7.3.2
Alternative name(s):
Acidic-type mitochondrial creatine kinase
Short name=Mia-CK
Ubiquitous mitochondrial creatine kinase
Short name=U-MtCK
Gene names
Name:CKMT1
OrganismSus scrofa (Pig) [Reference proteome]
Taxonomic identifier9823 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus

Protein attributes

Sequence length416 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa By similarity.

Catalytic activity

ATP + creatine = ADP + phosphocreatine.

Subunit structure

Exists as an octamer composed of four MTCK homodimers By similarity.

Subcellular location

Mitochondrion inner membrane; Peripheral membrane protein; Intermembrane side By similarity.

Miscellaneous

Mitochondrial creatine kinase binds cardiolipin By similarity.

Sequence similarities

Belongs to the ATP:guanido phosphotransferase family.

Contains 1 phosphagen kinase C-terminal domain.

Contains 1 phosphagen kinase N-terminal domain.

Ontologies

Keywords
   Cellular componentMembrane
Mitochondrion
Mitochondrion inner membrane
   DomainTransit peptide
   LigandATP-binding
Nucleotide-binding
   Molecular functionKinase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentmitochondrial inner membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

creatine kinase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 3939Mitochondrion By similarity
Chain40 – 416377Creatine kinase U-type, mitochondrial
PRO_0000211983

Regions

Domain44 – 13188Phosphagen kinase N-terminal
Domain158 – 400243Phosphagen kinase C-terminal
Nucleotide binding161 – 1655ATP By similarity
Nucleotide binding353 – 3586ATP By similarity
Region40 – 6324Cardiolipin-binding By similarity

Sites

Binding site2241ATP By similarity
Binding site2691ATP By similarity
Binding site3251ATP By similarity
Binding site3681ATP By similarity

Experimental info

Sequence conflict365 – 3662SI → ES in CAA23265. Ref.2
Sequence conflict396 – 3983CER → LANG in CAA23265. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q29577 [UniParc].

Last modified September 22, 2009. Version 2.
Checksum: 113919BF6571CF08

FASTA41646,950
        10         20         30         40         50         60 
MAGPFSRLLS ARPGLRLLAL AGAGSLAAGF LLRPEPIRAA SERRRQYPPS AEYPDLRKHN 

        70         80         90        100        110        120 
NCMASHLTPA VYARLCDKTT PTGWTLDQCI QTGVDNPGHP FIKTVGMVAG DEETYEVFAE 

       130        140        150        160        170        180 
LFDPVIQERH NGYDPRTMKH TTDLDASKIR SGFFDERYVL SSRVRTGRSI RGLSLPPACT 

       190        200        210        220        230        240 
RAERREVERV VVDALSGLKG DLAGRYYRLS EMTEAEQQQL IDDHFLFDKP VSPLLTAAGM 

       250        260        270        280        290        300 
ARDWPDARGI WHNNEKSFLI WVNEEDHTRV ISMEKGGNMK KVFERFCRGL KEVERLIQER 

       310        320        330        340        350        360 
GWEFMWNERL GYILTCPSNL GTGLRAGVHI KLPLLSKDSR FPKILENLRL QKRGTGGVDT 

       370        380        390        400        410 
AATGSIFDIS NLDRLGKSEV ELVQLVIDGV NYLIDCERRL ERGQDIRIPP PLPNKH 

« Hide

References

[1]Liu G.Y.
Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[2]"Evaluation and characterization of a porcine small intestine cDNA library: analysis of 839 clones."
Winteroe A.K., Fredholm M., Davies W.
Mamm. Genome 7:509-517(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 333-409.
Tissue: Small intestine.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
EU650786 mRNA. Translation: ACD02423.1.
F14801 mRNA. Translation: CAA23265.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Proteomic databases

PaxDbQ29577.
PRIDEQ29577.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Phylogenomic databases

eggNOGCOG3869.
HOGENOMHOG000232165.
HOVERGENHBG001339.

Family and domain databases

Gene3D1.10.135.10. 1 hit.
3.30.590.10. 1 hit.
InterProIPR022415. ATP-guanido_PTrfase_AS.
IPR022414. ATP-guanido_PTrfase_cat.
IPR022413. ATP-guanido_PTrfase_N.
IPR014746. Gln_synth/guanido_kin_cat_dom.
[Graphical view]
PfamPF00217. ATP-gua_Ptrans. 1 hit.
PF02807. ATP-gua_PtransN. 1 hit.
[Graphical view]
SUPFAMSSF48034. SSF48034. 1 hit.
PROSITEPS00112. PHOSPHAGEN_KINASE. 1 hit.
PS51510. PHOSPHAGEN_KINASE_C. 1 hit.
PS51509. PHOSPHAGEN_KINASE_N. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameKCRU_PIG
AccessionPrimary (citable) accession number: Q29577
Secondary accession number(s): B2ZF48
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: September 22, 2009
Last modified: November 13, 2013
This is version 67 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families