Reviewed, UniProtKB/Swiss-Prot Q29577 (KCRU_PIG)

Names and origin

Protein names

Recommended name:
    Creatine kinase U-type, mitochondrial
    EC=2.7.3.2
Alternative name(s):
    Ubiquitous mitochondrial creatine kinase
      Short name=U-MtCK
    Acidic-type mitochondrial creatine kinase
      Short name=Mia-CK

Gene names

Name:

CKMT1

Organism

Sus scrofa (Pig)

Taxonomic identifier

9823 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Suina › Suidae › Sus

Protein attributes

Sequence length	416 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa By similarity.
Catalytic activity	ATP + creatine = ADP + phosphocreatine.
Subunit structure	Exists as an octamer composed of four MTCK homodimers By similarity.
Subcellular location	Mitochondrion inner membrane; Peripheral membrane protein; Intermembrane side By similarity.
Miscellaneous	Mitochondrial creatine kinase binds cardiolipin By similarity.
Sequence similarities	Belongs to the ATP:guanido phosphotransferase family.

Ontologies

Keywords
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Transit peptide
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Gene Ontology (GO)
Cellular component	extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrial inner membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW creatine kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 39	39	Mitochondrion By similarity
	Chain	40 – 416	377	Creatine kinase U-type, mitochondrial		PRO_0000211983
Regions
	Nucleotide binding	161 – 165	5	ATP By similarity
	Nucleotide binding	353 – 358	6	ATP By similarity
	Region	40 – 63	24	Cardiolipin-binding By similarity
Sites
	Binding site	224	1	ATP By similarity
	Binding site	269	1	ATP By similarity
	Binding site	325	1	ATP By similarity
	Binding site	368	1	ATP By similarity
Experimental info
	Sequence conflict	365 – 366	2	SI → ES in CAA23265. Ref.2
	Sequence conflict	396 – 398	3	CER → LANG in CAA23265. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q29577-1 [UniParc]. Last modified September 22, 2009. Version 2. Checksum: 113919BF6571CF08	FASTA	416	46,950
	10 20 30 40 50 60 MAGPFSRLLS ARPGLRLLAL AGAGSLAAGF LLRPEPIRAA SERRRQYPPS AEYPDLRKHN 70 80 90 100 110 120 NCMASHLTPA VYARLCDKTT PTGWTLDQCI QTGVDNPGHP FIKTVGMVAG DEETYEVFAE 130 140 150 160 170 180 LFDPVIQERH NGYDPRTMKH TTDLDASKIR SGFFDERYVL SSRVRTGRSI RGLSLPPACT 190 200 210 220 230 240 RAERREVERV VVDALSGLKG DLAGRYYRLS EMTEAEQQQL IDDHFLFDKP VSPLLTAAGM 250 260 270 280 290 300 ARDWPDARGI WHNNEKSFLI WVNEEDHTRV ISMEKGGNMK KVFERFCRGL KEVERLIQER 310 320 330 340 350 360 GWEFMWNERL GYILTCPSNL GTGLRAGVHI KLPLLSKDSR FPKILENLRL QKRGTGGVDT 370 380 390 400 410 AATGSIFDIS NLDRLGKSEV ELVQLVIDGV NYLIDCERRL ERGQDIRIPP PLPNKH

References

[1]	Liu G.Y. Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[2]	"Evaluation and characterization of a porcine small intestine cDNA library: analysis of 839 clones." Winteroe A.K., Fredholm M., Davies W. Mamm. Genome 7:509-517(1996) [PubMed: 8672129] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 333-409. Tissue: Small intestine.

Cross-references

Sequence databases
EMBL GenBank DDBJ	EU650786 mRNA. Translation: ACD02423.1. F14801 mRNA. Translation: CAA23265.1.
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	ENSSSCT00000005194; ENSSSCP00000005068; ENSSSCG00000004703; Sus scrofa. [Genome view]
Phylogenomic databases
HOVERGEN	Q29577.
Enzyme and pathway databases
BRENDA	2.7.3.2. 249.
Family and domain databases
InterPro	IPR000749. ATP-guanido_PTrfase. IPR014746. Gln_synth/guanido_kin_cat_dom. [Graphical view]
Gene3D	G3DSA:1.10.135.10. ATP-gua_Ptrans. 1 hit. G3DSA:3.30.590.10. ATP-gua_Ptrans. 1 hit.
PANTHER	PTHR11547. ATP-gua_Ptrans. 1 hit.
Pfam	PF00217. ATP-gua_Ptrans. 1 hit. PF02807. ATP-gua_PtransN. 1 hit. [Graphical view]
PROSITE	PS00112. GUANIDO_KINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KCRU_PIG

Accession

Primary (citable) accession number: Q29577
Secondary accession number(s): B2ZF48

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	September 22, 2009
Last modified:	February 9, 2010
This is version 48 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)