ID   MAOX_PIG                Reviewed;         557 AA.
AC   Q29558;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   16-JUN-2009, entry version 57.
DE   RecName: Full=NADP-dependent malic enzyme;
DE            Short=NADP-ME;
DE            EC=1.1.1.40;
DE   AltName: Full=Malic enzyme 1;
DE   Flags: Fragment;
GN   Name=ME1;
OS   Sus scrofa (Pig).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Laurasiatheria; Cetartiodactyla; Suina; Suidae;
OC   Sus.
OX   NCBI_TaxID=9823;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=Large white; TISSUE=Muscle;
RX   MEDLINE=97032530; PubMed=8875889; DOI=10.1007/s003359900243;
RA   Nunes M., Lahbib-Mansais Y., Geffrotin C., Yerle M., Vaiman M.,
RA   Renard C.;
RT   "Swine cytosolic malic enzyme: cDNA cloning, sequencing, and
RT   localization.";
RL   Mamm. Genome 7:815-821(1996).
CC   -!- CATALYTIC ACTIVITY: (S)-malate + NADP(+) = pyruvate + CO(2) +
CC       NADPH.
CC   -!- COFACTOR: Divalent metal cations. Prefers magnesium or manganese
CC       (By similarity).
CC   -!- SUBUNIT: Homotetramer.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- SIMILARITY: Belongs to the malic enzymes family.
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DR   EMBL; X93016; CAA63599.1; -; mRNA.
DR   UniGene; Ssc.16336; -.
DR   HSSP; P40927; 1GQ2.
DR   HOVERGEN; Q29558; -.
DR   BRENDA; 1.1.1.40; 249.
DR   GO; GO:0005829; C:cytosol; ISS:UniProtKB.
DR   GO; GO:0004473; F:malate dehydrogenase (oxaloacetate-decarbox...; ISS:UniProtKB.
DR   GO; GO:0030145; F:manganese ion binding; ISS:UniProtKB.
DR   GO; GO:0051287; F:NAD or NADH binding; IEA:InterPro.
DR   GO; GO:0006108; P:malate metabolic process; ISS:UniProtKB.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   GO; GO:0009725; P:response to hormone stimulus; ISS:UniProtKB.
DR   InterPro; IPR015884; Malic_enzyme_CS.
DR   InterPro; IPR012301; Malic_N.
DR   InterPro; IPR012302; Malic_NAD_bd.
DR   InterPro; IPR001891; Malic_OxRdtase.
DR   InterPro; IPR016040; NAD(P)-bd_dom.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 1.
DR   Pfam; PF00390; malic; 1.
DR   Pfam; PF03949; Malic_M; 1.
DR   PRINTS; PR00072; MALOXRDTASE.
DR   PROSITE; PS00331; MALIC_ENZYMES; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; Metal-binding; NADP; Oxidoreductase.
FT   CHAIN        <1    557       NADP-dependent malic enzyme.
FT                                /FTId=PRO_0000160194.
FT   ACT_SITE     88     88       Proton donor (By similarity).
FT   ACT_SITE    159    159       Proton acceptor (By similarity).
FT   METAL       231    231       Divalent metal cation (By similarity).
FT   METAL       232    232       Divalent metal cation (By similarity).
FT   METAL       255    255       Divalent metal cation (By similarity).
FT   BINDING     141    141       NADP (By similarity).
FT   BINDING     255    255       NADP (By similarity).
FT   BINDING     394    394       NADP (By similarity).
FT   SITE        255    255       Important for activity (By similarity).
FT   NON_TER       1      1
SQ   SEQUENCE   557 AA;  62006 MW;  40692545F044BA99 CRC64;
     GYGLTRIPHL NKDLAFTLEE RQQLNIHGLL PPCFISQDIQ VLRVIKNFER LNSDFDRYLL
     LMDLQDRNEK LFYKVLMSDI EKFMPIVYTP TVGLACQQYS LAFRKPRGLF ISIHDRGHVA
     SVLNAWPEDV IKAVVVTDGE RILGLGDLGC NGMGIPVGKL ALYTACGGVN PQECLPVILD
     VGTENEELLK DPLYIGLRQR RVRGPEYDDF LDEFMEAVSS KYGMNCLIQF EDFANINAFR
     LLKKYQNQYC TFNDDIQGTA SVAVAGILAA LRITKNKLSD QTILFQGAGE AALGIAHLIV
     MAMEKEGVPK EKAIKKIWLV DSKGLIVKGR AALTNEKEEF AHEHEEMKNL EAIVQDIKPT
     ALIGVAAIGG AFSEQILKDM AAFNERPIIF ALSNPTSKAE CTAERGYTLT QGRAIFASGS
     PFDPVTLPSG QTLYPGQGNN SYVFPGVALA VVACGLRHIT DKIFLTTAEV IAQQVSDKHL
     EEGRLYPPLN TIRDVSLKIA EKIVRDAYQE KTATIYPEPS NKEAFVRSQM YSTDYDQILP
     DGYSWPEEAQ KIQTKLD
//