Q29558 (MAOX_PIG) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADP-dependent malic enzyme
Short name=NADP-ME
EC=1.1.1.40

Alternative name(s):
Malic enzyme 1

Gene names

Name:

ME1

Organism

Sus scrofa (Pig) [Complete proteome]

Taxonomic identifier

9823 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Suina › Suidae › Sus

Protein attributes

Sequence length	557 AA.
Sequence status	Fragment.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	(S)-malate + NADP+ = pyruvate + CO2 + NADPH.
Cofactor	Divalent metal cations. Prefers magnesium or manganese By similarity.
Subunit structure	Homotetramer.
Subcellular location	Cytoplasm.
Post-translational modification	ISGylated By similarity.
Sequence similarities	Belongs to the malic enzymes family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Metal-binding NADP
Molecular function	Oxidoreductase
PTM	Acetylation Ubl conjugation
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	malate metabolic process Inferred from sequence or structural similarity. Source: UniProtKB response to hormone stimulus Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	cytosol Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	NAD binding Inferred from electronic annotation. Source: InterPro malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+) activity Inferred from sequence or structural similarity. Source: UniProtKB malate dehydrogenase (oxaloacetate-decarboxylating) activity Inferred from electronic annotation. Source: InterPro manganese ion binding Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – 557	›557	NADP-dependent malic enzyme		PRO_0000160194
Sites
	Active site	88	1	Proton donor By similarity
	Active site	159	1	Proton acceptor By similarity
	Metal binding	231	1	Divalent metal cation By similarity
	Metal binding	232	1	Divalent metal cation By similarity
	Metal binding	255	1	Divalent metal cation By similarity
	Binding site	141	1	NADP By similarity
	Binding site	255	1	NADP By similarity
	Binding site	394	1	NADP By similarity
	Site	255	1	Important for activity By similarity
Amino acid modifications
	Modified residue	46	1	N6-acetyllysine By similarity
	Modified residue	70	1	N6-acetyllysine By similarity
Experimental info
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	Q29558 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 40692545F044BA99	FASTA	557	62,006
	10 20 30 40 50 60 GYGLTRIPHL NKDLAFTLEE RQQLNIHGLL PPCFISQDIQ VLRVIKNFER LNSDFDRYLL 70 80 90 100 110 120 LMDLQDRNEK LFYKVLMSDI EKFMPIVYTP TVGLACQQYS LAFRKPRGLF ISIHDRGHVA 130 140 150 160 170 180 SVLNAWPEDV IKAVVVTDGE RILGLGDLGC NGMGIPVGKL ALYTACGGVN PQECLPVILD 190 200 210 220 230 240 VGTENEELLK DPLYIGLRQR RVRGPEYDDF LDEFMEAVSS KYGMNCLIQF EDFANINAFR 250 260 270 280 290 300 LLKKYQNQYC TFNDDIQGTA SVAVAGILAA LRITKNKLSD QTILFQGAGE AALGIAHLIV 310 320 330 340 350 360 MAMEKEGVPK EKAIKKIWLV DSKGLIVKGR AALTNEKEEF AHEHEEMKNL EAIVQDIKPT 370 380 390 400 410 420 ALIGVAAIGG AFSEQILKDM AAFNERPIIF ALSNPTSKAE CTAERGYTLT QGRAIFASGS 430 440 450 460 470 480 PFDPVTLPSG QTLYPGQGNN SYVFPGVALA VVACGLRHIT DKIFLTTAEV IAQQVSDKHL 490 500 510 520 530 540 EEGRLYPPLN TIRDVSLKIA EKIVRDAYQE KTATIYPEPS NKEAFVRSQM YSTDYDQILP 550 DGYSWPEEAQ KIQTKLD

References

[1]	"Swine cytosolic malic enzyme: cDNA cloning, sequencing, and localization." Nunes M., Lahbib-Mansais Y., Geffrotin C., Yerle M., Vaiman M., Renard C. Mamm. Genome 7:815-821(1996) [PubMed: 8875889] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Large white. Tissue: Muscle.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X93016 mRNA. Translation: CAA63599.1.
UniGene	Ssc.16336.
3D structure databases
ProteinModelPortal	Q29558.
SMR	Q29558. Positions 1-541.
ModBase	Search...
Protein-protein interaction databases
STRING	Q29558.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
GeneTree	ENSGT00390000000754.
HOVERGEN	HBG000746.
OrthoDB	EOG44BB1T.
Family and domain databases
InterPro	IPR015884. Malic_enzyme_CS. IPR012301. Malic_N. IPR012302. Malic_NAD-bd. IPR001891. Malic_OxRdtase. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.10380. G3DSA:3.40.50.10380. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF00390. malic. 1 hit. PF03949. Malic_M. 1 hit. [Graphical view]
PIRSF	PIRSF000106. ME. 1 hit.
PRINTS	PR00072. MALOXRDTASE.
SMART	SM00919. Malic_M. 1 hit. [Graphical view]
PROSITE	PS00331. MALIC_ENZYMES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MAOX_PIG

Accession

Primary (citable) accession number: Q29558

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	October 19, 2011
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program