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Protein

Glycine N-methyltransferase

Gene

GNMT

Organism
Sus scrofa (Pig)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the methylation of glycine by using S-adenosylmethionine (AdoMet) to form N-methylglycine (sarcosine) with the concomitant production of S-adenosylhomocysteine (AdoHcy). Possible crucial role in the regulation of tissue concentration of AdoMet and of metabolism of methionine (By similarity).By similarity

Catalytic activityi

S-adenosyl-L-methionine + glycine = S-adenosyl-L-homocysteine + sarcosine.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei19S-adenosyl-L-methioninePROSITE-ProRule annotation1
Binding sitei28S-adenosyl-L-methioninePROSITE-ProRule annotation1
Binding sitei31SubstratePROSITE-ProRule annotation1
Binding sitei38S-adenosyl-L-methioninePROSITE-ProRule annotation1
Binding sitei62S-adenosyl-L-methionine; via carbonyl oxygenPROSITE-ProRule annotation1
Binding sitei83S-adenosyl-L-methioninePROSITE-ProRule annotation1
Binding sitei136S-adenosyl-L-methionine; via carbonyl oxygenPROSITE-ProRule annotation1
Binding sitei138SubstratePROSITE-ProRule annotation1
Binding sitei175SubstratePROSITE-ProRule annotation1
Binding sitei220SubstratePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Ligandi

Folate-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

SABIO-RKQ29555.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycine N-methyltransferase (EC:2.1.1.20)
Gene namesi
Name:GNMT
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
Proteomesi
  • UP000008227 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000087526‹1 – 292Glycine N-methyltransferaseAdd BLAST›292

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei7PhosphoserineBy similarity1
Modified residuei31PhosphotyrosineBy similarity1
Modified residuei43N6-succinyllysineBy similarity1
Modified residuei190N6-succinyllysineBy similarity1
Modified residuei195N6-succinyllysineBy similarity1
Modified residuei200N6-succinyllysineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ29555.
PeptideAtlasiQ29555.
PRIDEiQ29555.

Expressioni

Tissue specificityi

Abundant in liver.

Interactioni

Subunit structurei

Homotetramer.

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000001790.

Structurei

3D structure databases

ProteinModelPortaliQ29555.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni114 – 115S-adenosyl-L-methionine bindingPROSITE-ProRule annotation2

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. Glycine N-methyltransferase family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410IF1C. Eukaryota.
ENOG410ZWXN. LUCA.
HOVERGENiHBG051748.
InParanoidiQ29555.
KOiK00552.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR014369. Gly/Sar_N_MeTrfase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR16458. PTHR16458. 1 hit.
PIRSFiPIRSF000385. Gly_N-mtase. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51600. SAM_GNMT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Fragment.

Q29555-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
SVYRTRSLGV AAEGLPDQYA DGEAARVWQL YIGDTRSRTA EYKAWLLGLL
60 70 80 90 100
RQHGCQRVLD VACGTGVDSI MLVEEGFSVT SVDASDKMLK YALKERWNRR
110 120 130 140 150
HEPAFDKWVI EEANWMTLDK DVPKSPMGGF DAVICQGNSF AHLPDCRGDQ
160 170 180 190 200
SEHRLALKNI TSMVRSGGLL VIDHRNYDHI LSTGCAPPGK NIYYKSDLIK
210 220 230 240 250
DITTSVLTVN NKAHMVTLDY TVQVPGTGHR GSPGLSKFRL SYYPHCLASF
260 270 280 290
TELLQTAFGG NGQHSVLGDF KPYKPGQAYI PCYFIHVLRK TD
Length:292
Mass (Da):32,548
Last modified:November 1, 1996 - v1
Checksum:iAD460DE3910E0633
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Non-terminal residuei11

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13308 mRNA. Translation: BAA02568.1.
RefSeqiNP_001103889.2. NM_001110419.2.
UniGeneiSsc.16163.

Genome annotation databases

GeneIDi397444.
KEGGissc:397444.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13308 mRNA. Translation: BAA02568.1.
RefSeqiNP_001103889.2. NM_001110419.2.
UniGeneiSsc.16163.

3D structure databases

ProteinModelPortaliQ29555.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000001790.

Proteomic databases

PaxDbiQ29555.
PeptideAtlasiQ29555.
PRIDEiQ29555.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi397444.
KEGGissc:397444.

Organism-specific databases

CTDi27232.

Phylogenomic databases

eggNOGiENOG410IF1C. Eukaryota.
ENOG410ZWXN. LUCA.
HOVERGENiHBG051748.
InParanoidiQ29555.
KOiK00552.

Enzyme and pathway databases

SABIO-RKQ29555.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR014369. Gly/Sar_N_MeTrfase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR16458. PTHR16458. 1 hit.
PIRSFiPIRSF000385. Gly_N-mtase. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51600. SAM_GNMT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGNMT_PIG
AccessioniPrimary (citable) accession number: Q29555
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: October 5, 2016
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.