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Q29555 (GNMT_PIG) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 78. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glycine N-methyltransferase

EC=2.1.1.20
Gene names
Name:GNMT
OrganismSus scrofa (Pig) [Reference proteome]
Taxonomic identifier9823 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus

Protein attributes

Sequence length292 AA.
Sequence statusFragment.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes the methylation of glycine by using S-adenosylmethionine (AdoMet) to form N-methylglycine (sarcosine) with the concomitant production of S-adenosylhomocysteine (AdoHcy). Possible crucial role in the regulation of tissue concentration of AdoMet and of metabolism of methionine By similarity.

Catalytic activity

S-adenosyl-L-methionine + glycine = S-adenosyl-L-homocysteine + sarcosine.

Subunit structure

Homotetramer.

Subcellular location

Cytoplasm.

Tissue specificity

Abundant in liver.

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. Glycine N-methyltransferase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain‹1 – 292›292Glycine N-methyltransferase
PRO_0000087526

Regions

Region114 – 1152S-adenosyl-L-methionine binding By similarity

Sites

Binding site191S-adenosyl-L-methionine By similarity
Binding site281S-adenosyl-L-methionine By similarity
Binding site311Substrate By similarity
Binding site381S-adenosyl-L-methionine By similarity
Binding site621S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Binding site831S-adenosyl-L-methionine By similarity
Binding site1361S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Binding site1381Substrate By similarity
Binding site1751Substrate By similarity
Binding site2201Substrate By similarity

Amino acid modifications

Modified residue71Phosphoserine By similarity
Modified residue431N6-succinyllysine By similarity
Modified residue1901N6-succinyllysine By similarity
Modified residue1951N6-succinyllysine By similarity
Modified residue2001N6-succinyllysine By similarity

Experimental info

Non-terminal residue11

Sequences

Sequence LengthMass (Da)Tools
Q29555 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: AD460DE3910E0633

FASTA29232,548
        10         20         30         40         50         60 
SVYRTRSLGV AAEGLPDQYA DGEAARVWQL YIGDTRSRTA EYKAWLLGLL RQHGCQRVLD 

        70         80         90        100        110        120 
VACGTGVDSI MLVEEGFSVT SVDASDKMLK YALKERWNRR HEPAFDKWVI EEANWMTLDK 

       130        140        150        160        170        180 
DVPKSPMGGF DAVICQGNSF AHLPDCRGDQ SEHRLALKNI TSMVRSGGLL VIDHRNYDHI 

       190        200        210        220        230        240 
LSTGCAPPGK NIYYKSDLIK DITTSVLTVN NKAHMVTLDY TVQVPGTGHR GSPGLSKFRL 

       250        260        270        280        290 
SYYPHCLASF TELLQTAFGG NGQHSVLGDF KPYKPGQAYI PCYFIHVLRK TD 

« Hide

References

[1]"Mammalian glycine N-methyltransferases. Comparative kinetic and structural properties of the enzymes from human, rat, rabbit and pig livers."
Ogawa H., Gomi T., Fujioka M.
Comp. Biochem. Physiol. 106B:601-611(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Liver.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D13308 mRNA. Translation: BAA02568.1.
RefSeqNP_001103889.2. NM_001110419.2.
UniGeneSsc.16163.

3D structure databases

ProteinModelPortalQ29555.
SMRQ29555. Positions 3-291.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PaxDbQ29555.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID397444.
KEGGssc:397444.

Organism-specific databases

CTD27232.

Phylogenomic databases

eggNOGNOG78825.
HOVERGENHBG051748.
KOK00552.

Enzyme and pathway databases

SABIO-RKQ29555.

Family and domain databases

InterProIPR014369. Gly/Sar_N_MeTrfase.
[Graphical view]
PANTHERPTHR16458. PTHR16458. 1 hit.
PIRSFPIRSF000385. Gly_N-mtase. 1 hit.
PROSITEPS51600. SAM_GNMT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGNMT_PIG
AccessionPrimary (citable) accession number: Q29555
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: March 19, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families