Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q29555

- GNMT_PIG

UniProt

Q29555 - GNMT_PIG

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Glycine N-methyltransferase

Gene

GNMT

Organism
Sus scrofa (Pig)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

Catalyzes the methylation of glycine by using S-adenosylmethionine (AdoMet) to form N-methylglycine (sarcosine) with the concomitant production of S-adenosylhomocysteine (AdoHcy). Possible crucial role in the regulation of tissue concentration of AdoMet and of metabolism of methionine (By similarity).By similarity

Catalytic activityi

S-adenosyl-L-methionine + glycine = S-adenosyl-L-homocysteine + sarcosine.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei19 – 191S-adenosyl-L-methioninePROSITE-ProRule annotation
Binding sitei28 – 281S-adenosyl-L-methioninePROSITE-ProRule annotation
Binding sitei31 – 311SubstratePROSITE-ProRule annotation
Binding sitei38 – 381S-adenosyl-L-methioninePROSITE-ProRule annotation
Binding sitei62 – 621S-adenosyl-L-methionine; via carbonyl oxygenPROSITE-ProRule annotation
Binding sitei83 – 831S-adenosyl-L-methioninePROSITE-ProRule annotation
Binding sitei136 – 1361S-adenosyl-L-methionine; via carbonyl oxygenPROSITE-ProRule annotation
Binding sitei138 – 1381SubstratePROSITE-ProRule annotation
Binding sitei175 – 1751SubstratePROSITE-ProRule annotation
Binding sitei220 – 2201SubstratePROSITE-ProRule annotation

GO - Molecular functioni

  1. folic acid binding Source: UniProtKB-KW
  2. glycine binding Source: UniProtKB
  3. glycine N-methyltransferase activity Source: UniProtKB

GO - Biological processi

  1. S-adenosylmethionine metabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Ligandi

Folate-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

SABIO-RKQ29555.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycine N-methyltransferase (EC:2.1.1.20)
Gene namesi
Name:GNMT
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
ProteomesiUP000008227: Unplaced

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini‹1 – 292›292Glycine N-methyltransferasePRO_0000087526Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei7 – 71PhosphoserineBy similarity
Modified residuei43 – 431N6-succinyllysineBy similarity
Modified residuei190 – 1901N6-succinyllysineBy similarity
Modified residuei195 – 1951N6-succinyllysineBy similarity
Modified residuei200 – 2001N6-succinyllysineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ29555.

Expressioni

Tissue specificityi

Abundant in liver.

Interactioni

Subunit structurei

Homotetramer.

Structurei

3D structure databases

ProteinModelPortaliQ29555.
SMRiQ29555. Positions 3-291.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni114 – 1152S-adenosyl-L-methionine bindingPROSITE-ProRule annotation

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. Glycine N-methyltransferase family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG78825.
HOVERGENiHBG051748.
InParanoidiQ29555.
KOiK00552.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR014369. Gly/Sar_N_MeTrfase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PANTHERiPTHR16458. PTHR16458. 1 hit.
PIRSFiPIRSF000385. Gly_N-mtase. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51600. SAM_GNMT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Fragment.

Q29555-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
SVYRTRSLGV AAEGLPDQYA DGEAARVWQL YIGDTRSRTA EYKAWLLGLL
60 70 80 90 100
RQHGCQRVLD VACGTGVDSI MLVEEGFSVT SVDASDKMLK YALKERWNRR
110 120 130 140 150
HEPAFDKWVI EEANWMTLDK DVPKSPMGGF DAVICQGNSF AHLPDCRGDQ
160 170 180 190 200
SEHRLALKNI TSMVRSGGLL VIDHRNYDHI LSTGCAPPGK NIYYKSDLIK
210 220 230 240 250
DITTSVLTVN NKAHMVTLDY TVQVPGTGHR GSPGLSKFRL SYYPHCLASF
260 270 280 290
TELLQTAFGG NGQHSVLGDF KPYKPGQAYI PCYFIHVLRK TD
Length:292
Mass (Da):32,548
Last modified:November 1, 1996 - v1
Checksum:iAD460DE3910E0633
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei1 – 11

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13308 mRNA. Translation: BAA02568.1.
RefSeqiNP_001103889.2. NM_001110419.2.
UniGeneiSsc.16163.

Genome annotation databases

GeneIDi397444.
KEGGissc:397444.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13308 mRNA. Translation: BAA02568.1 .
RefSeqi NP_001103889.2. NM_001110419.2.
UniGenei Ssc.16163.

3D structure databases

ProteinModelPortali Q29555.
SMRi Q29555. Positions 3-291.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PaxDbi Q29555.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 397444.
KEGGi ssc:397444.

Organism-specific databases

CTDi 27232.

Phylogenomic databases

eggNOGi NOG78825.
HOVERGENi HBG051748.
InParanoidi Q29555.
KOi K00552.

Enzyme and pathway databases

SABIO-RK Q29555.

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
InterProi IPR014369. Gly/Sar_N_MeTrfase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view ]
PANTHERi PTHR16458. PTHR16458. 1 hit.
PIRSFi PIRSF000385. Gly_N-mtase. 1 hit.
SUPFAMi SSF53335. SSF53335. 1 hit.
PROSITEi PS51600. SAM_GNMT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Mammalian glycine N-methyltransferases. Comparative kinetic and structural properties of the enzymes from human, rat, rabbit and pig livers."
    Ogawa H., Gomi T., Fujioka M.
    Comp. Biochem. Physiol. 106B:601-611(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Liver.

Entry informationi

Entry nameiGNMT_PIG
AccessioniPrimary (citable) accession number: Q29555
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: October 29, 2014
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3