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Q29555

- GNMT_PIG

UniProt

Q29555 - GNMT_PIG

Protein

Glycine N-methyltransferase

Gene

GNMT

Organism
Sus scrofa (Pig)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 80 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Catalyzes the methylation of glycine by using S-adenosylmethionine (AdoMet) to form N-methylglycine (sarcosine) with the concomitant production of S-adenosylhomocysteine (AdoHcy). Possible crucial role in the regulation of tissue concentration of AdoMet and of metabolism of methionine By similarity.By similarity

    Catalytic activityi

    S-adenosyl-L-methionine + glycine = S-adenosyl-L-homocysteine + sarcosine.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei19 – 191S-adenosyl-L-methioninePROSITE-ProRule annotation
    Binding sitei28 – 281S-adenosyl-L-methioninePROSITE-ProRule annotation
    Binding sitei31 – 311SubstratePROSITE-ProRule annotation
    Binding sitei38 – 381S-adenosyl-L-methioninePROSITE-ProRule annotation
    Binding sitei62 – 621S-adenosyl-L-methionine; via carbonyl oxygenPROSITE-ProRule annotation
    Binding sitei83 – 831S-adenosyl-L-methioninePROSITE-ProRule annotation
    Binding sitei136 – 1361S-adenosyl-L-methionine; via carbonyl oxygenPROSITE-ProRule annotation
    Binding sitei138 – 1381SubstratePROSITE-ProRule annotation
    Binding sitei175 – 1751SubstratePROSITE-ProRule annotation
    Binding sitei220 – 2201SubstratePROSITE-ProRule annotation

    GO - Molecular functioni

    1. folic acid binding Source: UniProtKB-KW
    2. glycine binding Source: UniProtKB
    3. glycine N-methyltransferase activity Source: UniProtKB

    GO - Biological processi

    1. S-adenosylmethionine metabolic process Source: UniProtKB

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Ligandi

    Folate-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    SABIO-RKQ29555.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glycine N-methyltransferase (EC:2.1.1.20)
    Gene namesi
    Name:GNMT
    OrganismiSus scrofa (Pig)
    Taxonomic identifieri9823 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
    ProteomesiUP000008227: Unplaced

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini‹1 – 292›292Glycine N-methyltransferasePRO_0000087526Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei7 – 71PhosphoserineBy similarity
    Modified residuei43 – 431N6-succinyllysineBy similarity
    Modified residuei190 – 1901N6-succinyllysineBy similarity
    Modified residuei195 – 1951N6-succinyllysineBy similarity
    Modified residuei200 – 2001N6-succinyllysineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ29555.

    Expressioni

    Tissue specificityi

    Abundant in liver.

    Interactioni

    Subunit structurei

    Homotetramer.

    Structurei

    3D structure databases

    ProteinModelPortaliQ29555.
    SMRiQ29555. Positions 3-291.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni114 – 1152S-adenosyl-L-methionine bindingPROSITE-ProRule annotation

    Sequence similaritiesi

    Belongs to the class I-like SAM-binding methyltransferase superfamily. Glycine N-methyltransferase family.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG78825.
    HOVERGENiHBG051748.
    KOiK00552.

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    InterProiIPR014369. Gly/Sar_N_MeTrfase.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view]
    PANTHERiPTHR16458. PTHR16458. 1 hit.
    PIRSFiPIRSF000385. Gly_N-mtase. 1 hit.
    SUPFAMiSSF53335. SSF53335. 1 hit.
    PROSITEiPS51600. SAM_GNMT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Fragment.

    Q29555-1 [UniParc]FASTAAdd to Basket

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    SVYRTRSLGV AAEGLPDQYA DGEAARVWQL YIGDTRSRTA EYKAWLLGLL    50
    RQHGCQRVLD VACGTGVDSI MLVEEGFSVT SVDASDKMLK YALKERWNRR 100
    HEPAFDKWVI EEANWMTLDK DVPKSPMGGF DAVICQGNSF AHLPDCRGDQ 150
    SEHRLALKNI TSMVRSGGLL VIDHRNYDHI LSTGCAPPGK NIYYKSDLIK 200
    DITTSVLTVN NKAHMVTLDY TVQVPGTGHR GSPGLSKFRL SYYPHCLASF 250
    TELLQTAFGG NGQHSVLGDF KPYKPGQAYI PCYFIHVLRK TD 292
    Length:292
    Mass (Da):32,548
    Last modified:November 1, 1996 - v1
    Checksum:iAD460DE3910E0633
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Non-terminal residuei1 – 11

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D13308 mRNA. Translation: BAA02568.1.
    RefSeqiNP_001103889.2. NM_001110419.2.
    UniGeneiSsc.16163.

    Genome annotation databases

    GeneIDi397444.
    KEGGissc:397444.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D13308 mRNA. Translation: BAA02568.1 .
    RefSeqi NP_001103889.2. NM_001110419.2.
    UniGenei Ssc.16163.

    3D structure databases

    ProteinModelPortali Q29555.
    SMRi Q29555. Positions 3-291.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PaxDbi Q29555.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 397444.
    KEGGi ssc:397444.

    Organism-specific databases

    CTDi 27232.

    Phylogenomic databases

    eggNOGi NOG78825.
    HOVERGENi HBG051748.
    KOi K00552.

    Enzyme and pathway databases

    SABIO-RK Q29555.

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    InterProi IPR014369. Gly/Sar_N_MeTrfase.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view ]
    PANTHERi PTHR16458. PTHR16458. 1 hit.
    PIRSFi PIRSF000385. Gly_N-mtase. 1 hit.
    SUPFAMi SSF53335. SSF53335. 1 hit.
    PROSITEi PS51600. SAM_GNMT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Mammalian glycine N-methyltransferases. Comparative kinetic and structural properties of the enzymes from human, rat, rabbit and pig livers."
      Ogawa H., Gomi T., Fujioka M.
      Comp. Biochem. Physiol. 106B:601-611(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Liver.

    Entry informationi

    Entry nameiGNMT_PIG
    AccessioniPrimary (citable) accession number: Q29555
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 80 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3