Reviewed, UniProtKB/Swiss-Prot Q29552 (C11B1_PIG)

Names and origin

Protein names

Recommended name:
    Cytochrome P450 11B1, mitochondrial
    EC=1.14.15.4
Alternative name(s):
    CYPXIB1
    P450C11
    Steroid 11-beta-hydroxylase

Gene names

Name:

CYP11B1

Organism

Sus scrofa (Pig)

Taxonomic identifier

9823 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Suina › Suidae › Sus

Protein attributes

Sequence length	503 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Converts 11-deoxycorticosterone to corticosterone and 18-hydroxycorticosterone, and also produces aldosterone. May play a role in the biosynthesis of glucocorticoids as well as mineralocorticoids. In addition to this activity, the 18 or 19-hydroxylation of steroids and the aromatization of androstendione to estrone have also been ascribed to cytochrome P450 XIB.
Catalytic activity	A steroid + reduced adrenal ferredoxin + O(2) = an 11-beta-hydroxysteroid + oxidized adrenal ferredoxin + H(2)O.
Cofactor	Heme group By similarity.
Subcellular location	Mitochondrion membrane.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Biological process	Steroidogenesis
Cellular component	Membrane Mitochondrion
Domain	Transit peptide
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	C21-steroid hormone biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrial membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro steroid 11-beta-monooxygenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 24	24	Mitochondrion By similarity
	Chain	25 – 503	479	Cytochrome P450 11B1, mitochondrial		PRO_0000003602
Sites
	Metal binding	450	1	Iron (heme axial ligand) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q29552-1 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 4C40B1EEAAC49B05	FASTA	503	57,340
	10 20 30 40 50 60 MAIWAKAEAW LAGPWLALNR ARTLGTRAVL APKGVLPFEA IPQFPGKKWM RVLQLWREQG 70 80 90 100 110 120 FENNHLEMHQ TFQELGPIFR FDVGGRNMVL VMLPEDVERC QKVEGLHPQR DVPGPWLAYR 130 140 150 160 170 180 HLRGHKCGVF LLNGPTWRLD RLQLNPGVLS LQAMQKFTPL VDGVARDFSQ ALRARVMQNA 190 200 210 220 230 240 RGSLTLDIKP SIFRYTIEAS NLVLFGERLG LLAHQPNPES LDFIHALEVM FKSTVQLMFM 250 260 270 280 290 300 PRSLSRWTST GTWKEHFEAW DCIFQYANKA IQRLYQELTL GHPWHYSGVV AELLTHANMT 310 320 330 340 350 360 VDAIKANSID LTAGSVDTTA YPLLMTLFEL ARNPEVQQAL RQESLAAAAR ISENPQKAIT 370 380 390 400 410 420 ELPLLRAALK ETLRLYPVGI FLDRCVTSDL VLQNYHIPAG TLVKVLLYSL GRNPAVFARP 430 440 450 460 470 480 ERYHPQRWLD NQGSGTRFPH LAFGFGMRQC LGRRLAQVEM LLLLHHVLKN FLVETLVQED 490 500 IKMIYRFIMT PSTLPLLTFR AIS

References

[1]	"Cloning and expression of cytochrome P450(11 beta) of porcine adrenal cortex." Sun T., Zhao Y., Nonaka Y., Okamoto M. J. Steroid Biochem. Mol. Biol. 52:227-232(1995) [PubMed: 7696143] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Adrenal gland.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D38590 mRNA. Translation: BAA07600.1.
3D structure databases
HSSP	HSSP built from PDB template 1SCC based on UniProtKB P00189.
ModBase	Search...
Phylogenomic databases
HOVERGEN	Q29552.
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHER	PTHR19383. Cyt_P450. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00385. P450.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

C11B1_PIG

Accession

Primary (citable) accession number: Q29552

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	November 25, 2008
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)