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Protein

Succinyl-CoA:3-ketoacid coenzyme A transferase 1, mitochondrial

Gene

OXCT1

Organism
Sus scrofa (Pig)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Key enzyme for ketone body catabolism. Transfers the CoA moiety from succinate to acetoacetate. Formation of the enzyme-CoA intermediate proceeds via an unstable anhydride species formed between the carboxylate groups of the enzyme and substrate.

Catalytic activityi

Succinyl-CoA + a 3-oxo acid = succinate + a 3-oxoacyl-CoA.PROSITE-ProRule annotation2 Publications

Pathwayi: succinyl-CoA degradation

This protein is involved in step 1 of the subpathway that synthesizes acetoacetyl-CoA from succinyl-CoA.
Proteins known to be involved in this subpathway in this organism are:
  1. Succinyl-CoA:3-ketoacid-coenzyme A transferase, Succinyl-CoA:3-ketoacid coenzyme A transferase 1, mitochondrial (OXCT1)
This subpathway is part of the pathway succinyl-CoA degradation, which is itself part of Ketone metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes acetoacetyl-CoA from succinyl-CoA, the pathway succinyl-CoA degradation and in Ketone metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei3445-glutamyl coenzyme A thioester intermediatePROSITE-ProRule annotation1 Publication1

GO - Molecular functioni

  • 3-oxoacid CoA-transferase activity Source: UniProtKB
  • protein homodimerization activity Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Enzyme and pathway databases

BRENDAi2.8.3.5. 6170.
UniPathwayiUPA00929; UER00894.

Names & Taxonomyi

Protein namesi
Recommended name:
Succinyl-CoA:3-ketoacid coenzyme A transferase 1, mitochondrial (EC:2.8.3.5)
Alternative name(s):
3-oxoacid CoA-transferase 1
Somatic-type succinyl-CoA:3-oxoacid CoA-transferase
Short name:
SCOT-s
Gene namesi
Name:OXCT1
Synonyms:OXCT, SCOT
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
Proteomesi
  • UP000008227 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi67C → A: Decreases KM for acetoacetyl-CoA 3-fold. No effect on KM for acetoacetate, succinyl-CoA and succinate. 1 Publication1
Mutagenesisi67C → S: Decreases KM for acetoacetate and acetoacetyl-CoA 2-fold. Increases KM for succinate 2-fold. Decreases KM for succinyl-CoA nearly 4-fold. Strongly reduces catalytic activity. 1 Publication1
Mutagenesisi235C → S: Similar specific activity to wild-type. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 39Mitochondrion1 PublicationAdd BLAST39
ChainiPRO_000000241540 – 520Succinyl-CoA:3-ketoacid coenzyme A transferase 1, mitochondrialAdd BLAST481

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei170PhosphoserineBy similarity1
Modified residuei185N6-succinyllysineBy similarity1
Modified residuei418N6-succinyllysineBy similarity1
Modified residuei421N6-succinyllysineBy similarity1
Modified residuei455N6-succinyllysineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ29551.
PeptideAtlasiQ29551.
PRIDEiQ29551.

Expressioni

Gene expression databases

BgeeiENSSSCG00000016863.
GenevisibleiQ29551. SS.

Interactioni

Subunit structurei

Homodimer.6 Publications

GO - Molecular functioni

  • protein homodimerization activity Source: UniProtKB

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000017870.

Structurei

Secondary structure

1520
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi41 – 44Combined sources4
Helixi46 – 50Combined sources5
Beta strandi58 – 61Combined sources4
Helixi71 – 80Combined sources10
Beta strandi84 – 88Combined sources5
Helixi100 – 104Combined sources5
Beta strandi108 – 114Combined sources7
Helixi120 – 127Combined sources8
Beta strandi130 – 135Combined sources6
Helixi138 – 149Combined sources12
Beta strandi154 – 158Combined sources5
Turni159 – 162Combined sources4
Helixi164 – 167Combined sources4
Beta strandi171 – 174Combined sources4
Beta strandi178 – 183Combined sources6
Beta strandi189 – 192Combined sources4
Beta strandi195 – 201Combined sources7
Beta strandi205 – 216Combined sources12
Helixi225 – 227Combined sources3
Helixi231 – 234Combined sources4
Beta strandi237 – 249Combined sources13
Helixi256 – 258Combined sources3
Helixi263 – 265Combined sources3
Beta strandi268 – 271Combined sources4
Helixi302 – 309Combined sources8
Helixi310 – 312Combined sources3
Beta strandi317 – 321Combined sources5
Helixi325 – 329Combined sources5
Helixi330 – 332Combined sources3
Beta strandi339 – 343Combined sources5
Turni344 – 346Combined sources3
Beta strandi347 – 350Combined sources4
Helixi356 – 358Combined sources3
Beta strandi368 – 370Combined sources3
Beta strandi373 – 379Combined sources7
Helixi382 – 390Combined sources9
Beta strandi395 – 399Combined sources5
Beta strandi402 – 405Combined sources4
Beta strandi413 – 415Combined sources3
Turni416 – 418Combined sources3
Helixi426 – 429Combined sources4
Beta strandi435 – 440Combined sources6
Beta strandi443 – 445Combined sources3
Helixi446 – 448Combined sources3
Beta strandi450 – 455Combined sources6
Beta strandi461 – 464Combined sources4
Beta strandi468 – 470Combined sources3
Beta strandi472 – 479Combined sources8
Turni480 – 482Combined sources3
Beta strandi483 – 489Combined sources7
Helixi495 – 500Combined sources6
Beta strandi502 – 504Combined sources3
Beta strandi507 – 514Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1M3EX-ray2.50A/B/C/D40-520[»]
1O9LX-ray2.40A/B/C/D40-520[»]
1OOYX-ray1.70A/B40-520[»]
1OOZX-ray2.10A/B40-520[»]
1OPEX-ray2.50A/B40-520[»]
2NRBX-ray2.00A/B/C/D40-520[»]
2NRCX-ray2.05A/B/C/D40-520[»]
3K6MX-ray1.50A/B/C/D40-520[»]
3OXOX-ray2.30A/B/C/D/E/F/G/H40-517[»]
SMRiQ29551.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ29551.

Family & Domainsi

Sequence similaritiesi

Belongs to the 3-oxoacid CoA-transferase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG3822. Eukaryota.
COG1788. LUCA.
COG2057. LUCA.
HOVERGENiHBG002310.
InParanoidiQ29551.
KOiK01027.
OrthoDBiEOG091G0D3X.

Family and domain databases

InterProiIPR012792. 3-oxoacid_CoA-transf_A.
IPR012791. 3-oxoacid_CoA-transf_B.
IPR014388. 3-oxoacid_CoA-transferase.
IPR004165. CoA_trans_fam_I.
IPR004164. CoA_transf_AS.
IPR004163. CoA_transf_BS.
[Graphical view]
PANTHERiPTHR13707. PTHR13707. 1 hit.
PfamiPF01144. CoA_trans. 2 hits.
[Graphical view]
PIRSFiPIRSF000858. SCOT-t. 1 hit.
SMARTiSM00882. CoA_trans. 2 hits.
[Graphical view]
TIGRFAMsiTIGR02429. pcaI_scoA_fam. 1 hit.
TIGR02428. pcaJ_scoB_fam. 1 hit.
PROSITEiPS01273. COA_TRANSF_1. 1 hit.
PS01274. COA_TRANSF_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q29551-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAALTLLSSR LRLCASAYRS GGAWSQGCAG YFSTSTRRHT KFYTDAVEAV
60 70 80 90 100
KDIPNGATVL VGGFGLCGIP ENLIGALLKT GVKELTAVSN NAGVDNFGLG
110 120 130 140 150
LLLQSKQIKR MISSYVGENA EFERQYLAGE LEVELTPQGT LAERIRAGGA
160 170 180 190 200
GVPAFYTSTG YGTLVQEGGS PIKYNKDGSI AIASKPREVR EFNGQHFILE
210 220 230 240 250
EAIRGDFALV KAWKADQAGN VTFRKSARNF NLPMCKAAET TVVEVEEIVD
260 270 280 290 300
IGSFAPEDIH IPKIYVHRLV KGEKYEKRIE RLSVRKEEDV KTRSGKLGDN
310 320 330 340 350
VRERIIKRAA LEFEDGMYAN LGIGIPLLAS NFISPNMTVH LQSENGILGL
360 370 380 390 400
GPYPLQNEVD ADLINAGKET VTVLPGASYF SSDESFAMIR GGHVNLTMLG
410 420 430 440 450
AMQVSKYGDL ANWMIPGKLV KGMGGAMDLV SSAKTKVVVT MEHSAKGNAH
460 470 480 490 500
KIMEKCTLPL TGKQCVNRII TEKAVFDVDS KKGLTLIELW EGLTVDDIKK
510 520
STGCDFAVSP KLIPMQQVTT
Length:520
Mass (Da):56,338
Last modified:July 11, 2012 - v2
Checksum:i3DCFE430FE78C222
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti480S → R in AAA31019 (PubMed:1730685).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M80534 mRNA. Translation: AAA31019.1.
CU993811 Genomic DNA. No translation available.
FP017290 Genomic DNA. No translation available.
PIRiA41771.
RefSeqiNP_999103.1. NM_213938.1.
UniGeneiSsc.27309.

Genome annotation databases

GeneIDi396978.
KEGGissc:396978.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M80534 mRNA. Translation: AAA31019.1.
CU993811 Genomic DNA. No translation available.
FP017290 Genomic DNA. No translation available.
PIRiA41771.
RefSeqiNP_999103.1. NM_213938.1.
UniGeneiSsc.27309.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1M3EX-ray2.50A/B/C/D40-520[»]
1O9LX-ray2.40A/B/C/D40-520[»]
1OOYX-ray1.70A/B40-520[»]
1OOZX-ray2.10A/B40-520[»]
1OPEX-ray2.50A/B40-520[»]
2NRBX-ray2.00A/B/C/D40-520[»]
2NRCX-ray2.05A/B/C/D40-520[»]
3K6MX-ray1.50A/B/C/D40-520[»]
3OXOX-ray2.30A/B/C/D/E/F/G/H40-517[»]
SMRiQ29551.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000017870.

Proteomic databases

PaxDbiQ29551.
PeptideAtlasiQ29551.
PRIDEiQ29551.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi396978.
KEGGissc:396978.

Organism-specific databases

CTDi5019.

Phylogenomic databases

eggNOGiKOG3822. Eukaryota.
COG1788. LUCA.
COG2057. LUCA.
HOVERGENiHBG002310.
InParanoidiQ29551.
KOiK01027.
OrthoDBiEOG091G0D3X.

Enzyme and pathway databases

UniPathwayiUPA00929; UER00894.
BRENDAi2.8.3.5. 6170.

Miscellaneous databases

EvolutionaryTraceiQ29551.

Gene expression databases

BgeeiENSSSCG00000016863.
GenevisibleiQ29551. SS.

Family and domain databases

InterProiIPR012792. 3-oxoacid_CoA-transf_A.
IPR012791. 3-oxoacid_CoA-transf_B.
IPR014388. 3-oxoacid_CoA-transferase.
IPR004165. CoA_trans_fam_I.
IPR004164. CoA_transf_AS.
IPR004163. CoA_transf_BS.
[Graphical view]
PANTHERiPTHR13707. PTHR13707. 1 hit.
PfamiPF01144. CoA_trans. 2 hits.
[Graphical view]
PIRSFiPIRSF000858. SCOT-t. 1 hit.
SMARTiSM00882. CoA_trans. 2 hits.
[Graphical view]
TIGRFAMsiTIGR02429. pcaI_scoA_fam. 1 hit.
TIGR02428. pcaJ_scoB_fam. 1 hit.
PROSITEiPS01273. COA_TRANSF_1. 1 hit.
PS01274. COA_TRANSF_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSCOT1_PIG
AccessioniPrimary (citable) accession number: Q29551
Secondary accession number(s): F1SMH7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 11, 2012
Last modified: November 2, 2016
This is version 110 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.