Q29548 (HEXB_PIG) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-hexosaminidase subunit beta
EC=3.2.1.52

Alternative name(s):
65 kDa epididymal boar protein
Beta-N-acetylhexosaminidase subunit beta
Short name=Hexosaminidase subunit B
N-acetyl-beta-glucosaminidase subunit beta

Gene names

Name:

HEXB

Organism

Sus scrofa (Pig) [Reference proteome]

Taxonomic identifier

9823 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Cetartiodactyla › Suina › Suidae › Sus

Protein attributes

Sequence length	531 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Responsible for the degradation of GM2 gangliosides, and a variety of other molecules containing terminal N-acetyl hexosamines, in the brain and other tissues By similarity.
Catalytic activity	Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides.
Subcellular location	Lysosome.
Sequence similarities	Belongs to the glycosyl hydrolase 20 family.
Sequence caution	The sequence CAA63123.1 differs from that shown. Reason: Frameshift at positions 3 and 16.

Ontologies

Keywords
Cellular component	Lysosome
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	lysosome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	beta-N-acetylhexosaminidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Potential
	Chain	22 – 531	510	Beta-hexosaminidase subunit beta		PRO_0000012007
Sites
	Active site	329	1	Proton donor By similarity
Amino acid modifications
	Glycosylation	120	1	N-linked (GlcNAc...) Potential
	Glycosylation	164	1	N-linked (GlcNAc...) Potential
	Glycosylation	301	1	N-linked (GlcNAc...) Potential
	Disulfide bond	67 ↔ 111		By similarity
	Disulfide bond	283 ↔ 334		By similarity
	Disulfide bond	508 ↔ 525		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q29548 [UniParc]. Last modified May 10, 2005. Version 2. Checksum: F55D62D6937E51C5	FASTA	531	61,050
	10 20 30 40 50 60 MEVLPGLLRL LAALVVAERW ARDTSGAESL GLWPLPFAVD ISPRSLHLSP NNFFFGHSPT 70 80 90 100 110 120 SKAGSSCEIL QEAFRRYYDF IFGFYKWHQG SYQLCFGTEL QQLQVHVESE CDTFPSISSN 130 140 150 160 170 180 ESYVLHVKGP EALLRANTVW GALRGLETFS QLIYQDSYGT FTVNESEIID FPRFPHRGIL 190 200 210 220 230 240 IDTGRHFLSV KTIFKTLDAM AFNKFNVLHW HIVDDQSFPY QSINFGVLSS KGSYSLSHVY 250 260 270 280 290 300 TPNDVRMVIE YARIRGIRVM PEFDTPGHSR SWGKGQKDLL TPCYRKQVLS GTFGPINPIL 310 320 330 340 350 360 NTTYNFLSKF FKEISTVFPD EFIHIGGDEV DFDCWASNSE ILQFMQEKGF SQISLNSNLC 370 380 390 400 410 420 TVFKISNMIS AMKKRPIVWQ EAFDGRDKFM PGTVVQVWKI EDYKWEQSLI TKAGFPVILS 430 440 450 460 470 480 APWYLDLISY GQDWKNYYEV EPQDFPGSDK ERKRVLGGEA CLWGEYVDAT NLTPRLWPRA 490 500 510 520 530 SAVGERLWSH KDVRDIHDAY SRLTIHRCRM VRRGIAAEPL FTGYCNHEHR M

References

[1]	"Purification, cloning and sequencing analysis of B-N-acetyl-hexosaminidase from epididymal boar." Syntin P., Okamura N., Guillou F., Dacheux F., Dacheux J.-L. Submitted (OCT-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Epididymis.
[2]	"Characterization and identification of proteins secreted in the various regions of the adult boar epididymis." Syntin P., Dacheux F., Druart X., Gatti J.L., Okamura N., Dacheux J.-L. Biol. Reprod. 55:956-974(1996) [PubMed] [Europe PMC] [Abstract] Cited for: PARTIAL PROTEIN SEQUENCE. Tissue: Epididymis.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X92379 mRNA. Translation: CAA63123.1. Frameshift.
RefSeq	NP_999086.1. NM_213921.1.
UniGene	Ssc.3196.
3D structure databases
ProteinModelPortal	Q29548.
SMR	Q29548. Positions 31-527.
ModBase	Search...
Protein-protein interaction databases
STRING	9823.ENSSSCP00000014965.
Protein family/group databases
CAZy	GH20. Glycoside Hydrolase Family 20.
Proteomic databases
PaxDb	Q29548.
PRIDE	Q29548.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	396958.
KEGG	ssc:396958.
Organism-specific databases
CTD	3074.
Phylogenomic databases
eggNOG	COG3525.
HOGENOM	HOG000157972.
HOVERGEN	HBG005961.
KO	K12373.
OrthoDB	EOG42Z4Q7.
Family and domain databases
Gene3D	3.20.20.80. 1 hit.
InterPro	IPR025705. Beta_hexosaminidase_sua/sub. IPR015883. Glyco_hydro_20_cat-core. IPR015882. Glyco_hydro_20b. IPR013781. Glyco_hydro_catalytic_dom. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Pfam	PF00728. Glyco_hydro_20. 1 hit. PF02838. Glyco_hydro_20b. 1 hit. [Graphical view]
PIRSF	PIRSF001093. B-hxosamndse_ab_euk_. 1 hit.
PRINTS	PR00738. GLHYDRLASE20.
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HEXB_PIG

Accession

Primary (citable) accession number: Q29548

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 10, 2005
Last modified:	May 1, 2013
This is version 85 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program