Reviewed, UniProtKB/Swiss-Prot Q29524 (LIPL_SHEEP)

Names and origin

Protein names

Recommended name:
    Lipoprotein lipase
      Short name=LPL
    EC=3.1.1.34

Gene names

Name:

LPL

Organism

Ovis aries (Sheep)

Taxonomic identifier

9940 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Caprinae › Ovis

Protein attributes

Sequence length	478 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	The primary function of this lipase is the hydrolysis of triglycerides of circulating chylomicrons and very low density lipoproteins (VLDL). The enzyme functions in the presence of apolipoprotein C-2 on the luminal surface of vascular endothelium.
Catalytic activity	Triacylglycerol + H2O = diacylglycerol + a carboxylate.
Subunit structure	Homodimer. Interacts with apolipoprotein C-2. Interacts with GPIHBP1.
Subcellular location	Cell membrane; Lipid-anchor › GPI-anchor.
Post-translational modification	Tyrosine nitrated in vivo after lipopolysaccharide (LPS) challenge, which down-regulates lipoprotein lipase activity By similarity.
Sequence similarities	Belongs to the AB hydrolase superfamily. Lipase family. Contains 1 PLAT domain.

Ontologies

Keywords
Biological process	Lipid degradation
Cellular component	Cell membrane Chylomicron Membrane VLDL
Domain	Signal
Ligand	Heparin-binding
Molecular function	Hydrolase
PTM	Disulfide bond GPI-anchor Glycoprotein Lipoprotein Nitration
Gene Ontology (GO)
Biological process	lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	anchored to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell chylomicron Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW very-low-density lipoprotein particle Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	heparin binding Inferred from electronic annotation. Source: UniProtKB-KW lipoprotein lipase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 27	27	Potential
	Chain	28 – 478	451	Lipoprotein lipase		PRO_0000017781
Regions
	Domain	344 – 467	124	PLAT
	Region	322 – 334	13	Heparin-binding Potential
Sites
	Active site	162	1	Nucleophile
	Active site	186	1	Charge relay system
	Active site	271	1	Charge relay system
Amino acid modifications
	Modified residue	124	1	Nitrated tyrosine By similarity
	Modified residue	130	1	Nitrated tyrosine By similarity
	Modified residue	318	1	Nitrated tyrosine By similarity
	Glycosylation	73	1	N-linked (GlcNAc...) Potential
	Glycosylation	389	1	N-linked (GlcNAc...) Potential
	Disulfide bond	57 ↔ 70		By similarity
	Disulfide bond	246 ↔ 269		By similarity
	Disulfide bond	294 ↔ 313		By similarity
	Disulfide bond	305 ↔ 308		By similarity
	Disulfide bond	448 ↔ 468		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q29524-1 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 10F41EE1FFB9C882	FASTA	478	53,588
	10 20 30 40 50 60 MESKVLLLLA LSVWLQSLTV SRGGLVAADR ITRGKDFRDI ESKFALRTPE DTAEDTCHLI 70 80 90 100 110 120 PGVTESVANC HFNHSSKTFV VIHGWTVTGM YESWVPKLVA ALYKREPDSN VIVVDWLSRA 130 140 150 160 170 180 QQHYPVSAGY TKLVGQDVAK FMNWMADEFN YPLGNVHLLG YSLGAHAAGI AGSLTNKKVN 190 200 210 220 230 240 RITGLDPAGP NFEYAEAPSR LSPDDADFVD VLHTFTRGSP GRSIGIQKPV GHVDIYPNGG 250 260 270 280 290 300 TFQPGCNIGE ALRVIAERGL GDVDQLVKCS HERSVHLFID SLLNEENPSK AYRCNSKEAF 310 320 330 340 350 360 EKGLCLSCRK NRCNNMGYEI NKVRAKRSSK MYLKTRSQMP YKVFHYQVKI HFSGTESNTY 370 380 390 400 410 420 TNQAFEISLY GTVAESENIP FTLPEVSTNK TYSFLLYTEV DIGELLMLKL KWISDSYFSW 430 440 450 460 470 SNWWSSPGFD IGKIRVKAGE TQKKVIFCSR EKMSYLQKGK SPVIFVKCHD KSLNRKSG

References

[1]

"Cloning and sequencing of a full length cDNA encoding ovine lipoprotein lipase." Edwards W.D., Daniels S.W., Page R.A., Volpe C.P., Kille P., Sweeney G.E., Cryer A. Biochim. Biophys. Acta 1172:167-170(1993) [PubMed: 8439555] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Welsh Mountain/Dorset. Tissue: Adipose tissue.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X68308 mRNA. Translation: CAA48384.1.
PIR	S29846.
RefSeq	NP_001009394.1.
UniGene	Oar.13297
3D structure databases
SMR	Q29524. Positions 38-454.
ModBase	Search...
Genome annotation databases
GeneID	443408.
Organism-specific databases
CTD	443408.
Phylogenomic databases
HOVERGEN	Q29524.
Enzyme and pathway databases
BRENDA	3.1.1.34. 271.
Family and domain databases
InterPro	IPR000734. Lipase. IPR008976. Lipase_LipOase. IPR013818. Lipase_N. IPR002330. Lipo_Lipase. IPR001024. LipOase_LH2. IPR016272. Lipoprotein_lipase_LIPH. [Graphical view]
PANTHER	PTHR11610. Lipase. 1 hit. PTHR11610:SF3. Lipase_lipo. 1 hit.
Pfam	PF00151. Lipase. 1 hit. PF01477. PLAT. 1 hit. [Graphical view]
PIRSF	PIRSF000865. Lipoprotein_lipase_LIPH. 1 hit.
PRINTS	PR00822. LIPOLIPASE. PR00821. TAGLIPASE.
SMART	SM00308. LH2. 1 hit. [Graphical view]
TIGRFAMs	TIGR03230. lipo_lipase. 1 hit.
PROSITE	PS00120. LIPASE_SER. 1 hit. PS50095. PLAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LIPL_SHEEP

Accession

Primary (citable) accession number: Q29524

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	February 9, 2010
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)