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Reviewed, UniProtKB/Swiss-Prot Q29513 (GNMT_RABIT)

Last modified February 9, 2010. Version 54. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Glycine N-methyltransferase
    EC=2.1.1.20
Gene names
Name: GNMT
OrganismOryctolagus cuniculus (Rabbit)
Taxonomic identifier9986 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus

Protein attributes

Sequence length292 AA.
Sequence statusFragment.
Protein existenceEvidence at transcript level.

General annotation (Comments)

Function

Catalyzes the methylation of glycine by using S-adenosylmethionine (AdoMet) to form N-methylglycine (sarcosine) with the concomitant production of S-adenosylhomocysteine (AdoHcy). Possible crucial role in the regulation of tissue concentration of AdoMet and of metabolism of methionine.

Catalytic activity

S-adenosyl-L-methionine + glycine = S-adenosyl-L-homocysteine + sarcosine.

Subunit structure

Homotetramer.

Subcellular location

Cytoplasm.

Tissue specificity

Abundant in liver.

Sequence similarities

Belongs to the glycine N-methyltransferase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain‹1 – 292›292Glycine N-methyltransferase
PRO_0000087527

Regions

Region115 – 1162S-adenosyl-L-methionine binding By similarity

Sites

Binding site201S-adenosyl-L-methionine By similarity
Binding site291S-adenosyl-L-methionine By similarity
Binding site321Substrate By similarity
Binding site391S-adenosyl-L-methionine By similarity
Binding site631S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Binding site841S-adenosyl-L-methionine By similarity
Binding site1371S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Binding site1391Substrate By similarity
Binding site1761Substrate By similarity
Binding site2201Substrate By similarity

Experimental info

Non-terminal residue11

Sequences

Sequence LengthMass (Da)Tools
Q29513-1 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: CF36EF0379313CDA

FASTA29232,626
        10         20         30         40         50         60 
DSVYRTRSLG VAAEGLPDQY ADGEAARVWQ LYIGDTRSRT SEYKTWLLGL LRQHGCRRVL 

        70         80         90        100        110        120 
DVACGTGVDS IMLVEEGFSV TSVDASDKML KYALKERWNR RHEPAFDKWV IEEANWMTLD 

       130        140        150        160        170        180 
KDVPQPAGGG FDAVICLGNS FAHLPDCKGD QSEHRQALKN IASMVRTGGL LVIDHRNYDH 

       190        200        210        220        230        240 
ILSTLCAPGK NIYYKSDLTK DVTTSVLTVN NKAHMVTLDY TVQVPAAGQD GPPSLSKFRL 

       250        260        270        280        290 
SYYPHCLAPF TEFLRAAFEG KCQHSVLGDF KPYQPGQAYV PCYFIHVLKR TA 

« Hide

References

[1]"Mammalian glycine N-methyltransferases. Comparative kinetic and structural properties of the enzymes from human, rat, rabbit and pig livers."
Ogawa H., Gomi T., Fujioka M.
Comp. Biochem. Physiol. 106B:601-611(1993) [PubMed: 8281755] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Liver.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D13307 mRNA. Translation: BAA02567.1.
UniGeneOcu.2139

3D structure databases

SMRQ29513. Positions 1-292.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ29513.

Genome annotation databases

EnsemblENSOCUT00000003855; ENSOCUP00000003341; ENSOCUG00000003858; Oryctolagus cuniculus. [Genome view]

Phylogenomic databases

eggNOGmaNOG08489.
HOVERGENQ29513.

Enzyme and pathway databases

BRENDA2.1.1.20. 255.

Family and domain databases

InterProIPR014369. Gly_N_MeTrfase.
[Graphical view]
PIRSFPIRSF000385. Gly_N-mtase. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGNMT_RABIT
AccessionPrimary (citable) accession number: Q29513
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: February 9, 2010
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents