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Protein

Glycine N-methyltransferase

Gene

GNMT

Organism
Oryctolagus cuniculus (Rabbit)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the methylation of glycine by using S-adenosylmethionine (AdoMet) to form N-methylglycine (sarcosine) with the concomitant production of S-adenosylhomocysteine (AdoHcy). Possible crucial role in the regulation of tissue concentration of AdoMet and of metabolism of methionine.

Catalytic activityi

S-adenosyl-L-methionine + glycine = S-adenosyl-L-homocysteine + sarcosine.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei20 – 201S-adenosyl-L-methioninePROSITE-ProRule annotation
Binding sitei29 – 291S-adenosyl-L-methioninePROSITE-ProRule annotation
Binding sitei32 – 321SubstratePROSITE-ProRule annotation
Binding sitei39 – 391S-adenosyl-L-methioninePROSITE-ProRule annotation
Binding sitei63 – 631S-adenosyl-L-methionine; via carbonyl oxygenPROSITE-ProRule annotation
Binding sitei84 – 841S-adenosyl-L-methioninePROSITE-ProRule annotation
Binding sitei137 – 1371S-adenosyl-L-methionine; via carbonyl oxygenPROSITE-ProRule annotation
Binding sitei139 – 1391SubstratePROSITE-ProRule annotation
Binding sitei176 – 1761SubstratePROSITE-ProRule annotation
Binding sitei220 – 2201SubstratePROSITE-ProRule annotation

GO - Molecular functioni

  1. folic acid binding Source: UniProtKB-KW
  2. glycine binding Source: UniProtKB
  3. glycine N-methyltransferase activity Source: UniProtKB

GO - Biological processi

  1. S-adenosylmethionine metabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Ligandi

Folate-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

SABIO-RKQ29513.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycine N-methyltransferase (EC:2.1.1.20)
Gene namesi
Name:GNMT
OrganismiOryctolagus cuniculus (Rabbit)
Taxonomic identifieri9986 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus
ProteomesiUP000001811: Unplaced

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini‹1 – 292›292Glycine N-methyltransferasePRO_0000087527Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei8 – 81PhosphoserineBy similarity
Modified residuei44 – 441N6-succinyllysineBy similarity
Modified residuei190 – 1901N6-succinyllysineBy similarity
Modified residuei195 – 1951N6-succinyllysineBy similarity
Modified residuei200 – 2001N6-succinyllysineBy similarity

Keywords - PTMi

Phosphoprotein

Expressioni

Tissue specificityi

Abundant in liver.

Interactioni

Subunit structurei

Homotetramer.

Protein-protein interaction databases

STRINGi9986.ENSOCUP00000003341.

Structurei

3D structure databases

ProteinModelPortaliQ29513.
SMRiQ29513. Positions 1-292.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni115 – 1162S-adenosyl-L-methionine bindingPROSITE-ProRule annotation

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. Glycine N-methyltransferase family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG78825.
HOGENOMiHOG000276537.
HOVERGENiHBG051748.
InParanoidiQ29513.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR014369. Gly/Sar_N_MeTrfase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR16458. PTHR16458. 1 hit.
PIRSFiPIRSF000385. Gly_N-mtase. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51600. SAM_GNMT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Fragment.

Q29513-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
DSVYRTRSLG VAAEGLPDQY ADGEAARVWQ LYIGDTRSRT SEYKTWLLGL
60 70 80 90 100
LRQHGCRRVL DVACGTGVDS IMLVEEGFSV TSVDASDKML KYALKERWNR
110 120 130 140 150
RHEPAFDKWV IEEANWMTLD KDVPQPAGGG FDAVICLGNS FAHLPDCKGD
160 170 180 190 200
QSEHRQALKN IASMVRTGGL LVIDHRNYDH ILSTLCAPGK NIYYKSDLTK
210 220 230 240 250
DVTTSVLTVN NKAHMVTLDY TVQVPAAGQD GPPSLSKFRL SYYPHCLAPF
260 270 280 290
TEFLRAAFEG KCQHSVLGDF KPYQPGQAYV PCYFIHVLKR TA
Length:292
Mass (Da):32,626
Last modified:November 1, 1996 - v1
Checksum:iCF36EF0379313CDA
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei1 – 11

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13307 mRNA. Translation: BAA02567.1.
UniGeneiOcu.2139.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13307 mRNA. Translation: BAA02567.1.
UniGeneiOcu.2139.

3D structure databases

ProteinModelPortaliQ29513.
SMRiQ29513. Positions 1-292.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9986.ENSOCUP00000003341.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiNOG78825.
HOGENOMiHOG000276537.
HOVERGENiHBG051748.
InParanoidiQ29513.

Enzyme and pathway databases

SABIO-RKQ29513.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR014369. Gly/Sar_N_MeTrfase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR16458. PTHR16458. 1 hit.
PIRSFiPIRSF000385. Gly_N-mtase. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51600. SAM_GNMT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Mammalian glycine N-methyltransferases. Comparative kinetic and structural properties of the enzymes from human, rat, rabbit and pig livers."
    Ogawa H., Gomi T., Fujioka M.
    Comp. Biochem. Physiol. 106B:601-611(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Liver.

Entry informationi

Entry nameiGNMT_RABIT
AccessioniPrimary (citable) accession number: Q29513
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: January 7, 2015
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.