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Q29513 (GNMT_RABIT) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 77. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glycine N-methyltransferase

EC=2.1.1.20
Gene names
Name:GNMT
OrganismOryctolagus cuniculus (Rabbit) [Reference proteome]
Taxonomic identifier9986 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus

Protein attributes

Sequence length292 AA.
Sequence statusFragment.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes the methylation of glycine by using S-adenosylmethionine (AdoMet) to form N-methylglycine (sarcosine) with the concomitant production of S-adenosylhomocysteine (AdoHcy). Possible crucial role in the regulation of tissue concentration of AdoMet and of metabolism of methionine.

Catalytic activity

S-adenosyl-L-methionine + glycine = S-adenosyl-L-homocysteine + sarcosine.

Subunit structure

Homotetramer.

Subcellular location

Cytoplasm.

Tissue specificity

Abundant in liver.

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. Glycine N-methyltransferase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain‹1 – 292›292Glycine N-methyltransferase
PRO_0000087527

Regions

Region115 – 1162S-adenosyl-L-methionine binding By similarity

Sites

Binding site201S-adenosyl-L-methionine By similarity
Binding site291S-adenosyl-L-methionine By similarity
Binding site321Substrate By similarity
Binding site391S-adenosyl-L-methionine By similarity
Binding site631S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Binding site841S-adenosyl-L-methionine By similarity
Binding site1371S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Binding site1391Substrate By similarity
Binding site1761Substrate By similarity
Binding site2201Substrate By similarity

Amino acid modifications

Modified residue81Phosphoserine By similarity
Modified residue441N6-succinyllysine By similarity
Modified residue1901N6-succinyllysine By similarity
Modified residue1951N6-succinyllysine By similarity
Modified residue2001N6-succinyllysine By similarity

Experimental info

Non-terminal residue11

Sequences

Sequence LengthMass (Da)Tools
Q29513 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: CF36EF0379313CDA

FASTA29232,626
        10         20         30         40         50         60 
DSVYRTRSLG VAAEGLPDQY ADGEAARVWQ LYIGDTRSRT SEYKTWLLGL LRQHGCRRVL 

        70         80         90        100        110        120 
DVACGTGVDS IMLVEEGFSV TSVDASDKML KYALKERWNR RHEPAFDKWV IEEANWMTLD 

       130        140        150        160        170        180 
KDVPQPAGGG FDAVICLGNS FAHLPDCKGD QSEHRQALKN IASMVRTGGL LVIDHRNYDH 

       190        200        210        220        230        240 
ILSTLCAPGK NIYYKSDLTK DVTTSVLTVN NKAHMVTLDY TVQVPAAGQD GPPSLSKFRL 

       250        260        270        280        290 
SYYPHCLAPF TEFLRAAFEG KCQHSVLGDF KPYQPGQAYV PCYFIHVLKR TA 

« Hide

References

[1]"Mammalian glycine N-methyltransferases. Comparative kinetic and structural properties of the enzymes from human, rat, rabbit and pig livers."
Ogawa H., Gomi T., Fujioka M.
Comp. Biochem. Physiol. 106B:601-611(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Liver.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D13307 mRNA. Translation: BAA02567.1.
UniGeneOcu.2139.

3D structure databases

ProteinModelPortalQ29513.
SMRQ29513. Positions 1-292.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9986.ENSOCUP00000003341.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Phylogenomic databases

eggNOGNOG78825.
HOGENOMHOG000276537.
HOVERGENHBG051748.

Enzyme and pathway databases

SABIO-RKQ29513.

Family and domain databases

InterProIPR014369. Gly/Sar_N_MeTrfase.
[Graphical view]
PANTHERPTHR16458. PTHR16458. 1 hit.
PIRSFPIRSF000385. Gly_N-mtase. 1 hit.
PROSITEPS51600. SAM_GNMT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGNMT_RABIT
AccessionPrimary (citable) accession number: Q29513
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: March 19, 2014
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families