Reviewed, UniProtKB/Swiss-Prot Q29513 (GNMT_RABIT)

Names and origin

Protein names

Recommended name:
    Glycine N-methyltransferase
    EC=2.1.1.20

Gene names

Name:

GNMT

Organism

Oryctolagus cuniculus (Rabbit)

Taxonomic identifier

9986 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Lagomorpha › Leporidae › Oryctolagus

Protein attributes

Sequence length	292 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the methylation of glycine by using S-adenosylmethionine (AdoMet) to form N-methylglycine (sarcosine) with the concomitant production of S-adenosylhomocysteine (AdoHcy). Possible crucial role in the regulation of tissue concentration of AdoMet and of metabolism of methionine.
Catalytic activity	S-adenosyl-L-methionine + glycine = S-adenosyl-L-homocysteine + sarcosine.
Subunit structure	Homotetramer.
Subcellular location	Cytoplasm.
Tissue specificity	Abundant in liver.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Folate-binding S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Biological process	methionine metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	folic acid binding Inferred from electronic annotation. Source: UniProtKB-KW glycine N-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – 292	›292	Glycine N-methyltransferase		PRO_0000087527
Experimental info
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	Q29513-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: CF36EF0379313CDA	FASTA	292	32,626
	10 20 30 40 50 60 DSVYRTRSLG VAAEGLPDQY ADGEAARVWQ LYIGDTRSRT SEYKTWLLGL LRQHGCRRVL 70 80 90 100 110 120 DVACGTGVDS IMLVEEGFSV TSVDASDKML KYALKERWNR RHEPAFDKWV IEEANWMTLD 130 140 150 160 170 180 KDVPQPAGGG FDAVICLGNS FAHLPDCKGD QSEHRQALKN IASMVRTGGL LVIDHRNYDH 190 200 210 220 230 240 ILSTLCAPGK NIYYKSDLTK DVTTSVLTVN NKAHMVTLDY TVQVPAAGQD GPPSLSKFRL 250 260 270 280 290 SYYPHCLAPF TEFLRAAFEG KCQHSVLGDF KPYQPGQAYV PCYFIHVLKR TA

References

[1]	"Mammalian glycine N-methyltransferases. Comparative kinetic and structural properties of the enzymes from human, rat, rabbit and pig livers." Ogawa H., Gomi T., Fujioka M. Comp. Biochem. Physiol. 106B:601-611(1993) [PubMed: 8281755] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D13307 mRNA. Translation: BAA02567.1.
UniGene	Ocu.2139
3D structure databases
HSSP	HSSP built from PDB template 1XVA based on UniProtKB P13255.
SMR	Q29513. Positions 1-292.
ModBase	Search...
Genome annotation databases
Ensembl	ENSOCUG00000003858. Oryctolagus cuniculus. [Contig view]
Phylogenomic databases
HOVERGEN	Q29513.
Enzyme and pathway databases
BRENDA	2.1.1.20. 255.
Family and domain databases
InterPro	IPR014369. Gly_N_MeTrfase. IPR013217. Methyltransf_12. [Graphical view]
Pfam	PF08242. Methyltransf_12. 1 hit. [Graphical view]
PIRSF	PIRSF000385. Gly_N-mtase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GNMT_RABIT

Accession

Primary (citable) accession number: Q29513

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)