Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q29513

- GNMT_RABIT

UniProt

Q29513 - GNMT_RABIT

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Glycine N-methyltransferase

Gene

GNMT

Organism
Oryctolagus cuniculus (Rabbit)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Catalyzes the methylation of glycine by using S-adenosylmethionine (AdoMet) to form N-methylglycine (sarcosine) with the concomitant production of S-adenosylhomocysteine (AdoHcy). Possible crucial role in the regulation of tissue concentration of AdoMet and of metabolism of methionine.

Catalytic activityi

S-adenosyl-L-methionine + glycine = S-adenosyl-L-homocysteine + sarcosine.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei20 – 201S-adenosyl-L-methioninePROSITE-ProRule annotation
Binding sitei29 – 291S-adenosyl-L-methioninePROSITE-ProRule annotation
Binding sitei32 – 321SubstratePROSITE-ProRule annotation
Binding sitei39 – 391S-adenosyl-L-methioninePROSITE-ProRule annotation
Binding sitei63 – 631S-adenosyl-L-methionine; via carbonyl oxygenPROSITE-ProRule annotation
Binding sitei84 – 841S-adenosyl-L-methioninePROSITE-ProRule annotation
Binding sitei137 – 1371S-adenosyl-L-methionine; via carbonyl oxygenPROSITE-ProRule annotation
Binding sitei139 – 1391SubstratePROSITE-ProRule annotation
Binding sitei176 – 1761SubstratePROSITE-ProRule annotation
Binding sitei220 – 2201SubstratePROSITE-ProRule annotation

GO - Molecular functioni

  1. folic acid binding Source: UniProtKB-KW
  2. glycine binding Source: UniProtKB
  3. glycine N-methyltransferase activity Source: UniProtKB

GO - Biological processi

  1. S-adenosylmethionine metabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Ligandi

Folate-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

SABIO-RKQ29513.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycine N-methyltransferase (EC:2.1.1.20)
Gene namesi
Name:GNMT
OrganismiOryctolagus cuniculus (Rabbit)
Taxonomic identifieri9986 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus
ProteomesiUP000001811: Unplaced

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini‹1 – 292›292Glycine N-methyltransferasePRO_0000087527Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei8 – 81PhosphoserineBy similarity
Modified residuei44 – 441N6-succinyllysineBy similarity
Modified residuei190 – 1901N6-succinyllysineBy similarity
Modified residuei195 – 1951N6-succinyllysineBy similarity
Modified residuei200 – 2001N6-succinyllysineBy similarity

Keywords - PTMi

Phosphoprotein

Expressioni

Tissue specificityi

Abundant in liver.

Interactioni

Subunit structurei

Homotetramer.

Protein-protein interaction databases

STRINGi9986.ENSOCUP00000003341.

Structurei

3D structure databases

ProteinModelPortaliQ29513.
SMRiQ29513. Positions 1-292.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni115 – 1162S-adenosyl-L-methionine bindingPROSITE-ProRule annotation

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. Glycine N-methyltransferase family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG78825.
HOGENOMiHOG000276537.
HOVERGENiHBG051748.
InParanoidiQ29513.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR014369. Gly/Sar_N_MeTrfase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PANTHERiPTHR16458. PTHR16458. 1 hit.
PIRSFiPIRSF000385. Gly_N-mtase. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51600. SAM_GNMT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Fragment.

Q29513-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
DSVYRTRSLG VAAEGLPDQY ADGEAARVWQ LYIGDTRSRT SEYKTWLLGL
60 70 80 90 100
LRQHGCRRVL DVACGTGVDS IMLVEEGFSV TSVDASDKML KYALKERWNR
110 120 130 140 150
RHEPAFDKWV IEEANWMTLD KDVPQPAGGG FDAVICLGNS FAHLPDCKGD
160 170 180 190 200
QSEHRQALKN IASMVRTGGL LVIDHRNYDH ILSTLCAPGK NIYYKSDLTK
210 220 230 240 250
DVTTSVLTVN NKAHMVTLDY TVQVPAAGQD GPPSLSKFRL SYYPHCLAPF
260 270 280 290
TEFLRAAFEG KCQHSVLGDF KPYQPGQAYV PCYFIHVLKR TA
Length:292
Mass (Da):32,626
Last modified:November 1, 1996 - v1
Checksum:iCF36EF0379313CDA
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei1 – 11

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13307 mRNA. Translation: BAA02567.1.
UniGeneiOcu.2139.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13307 mRNA. Translation: BAA02567.1 .
UniGenei Ocu.2139.

3D structure databases

ProteinModelPortali Q29513.
SMRi Q29513. Positions 1-292.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9986.ENSOCUP00000003341.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Phylogenomic databases

eggNOGi NOG78825.
HOGENOMi HOG000276537.
HOVERGENi HBG051748.
InParanoidi Q29513.

Enzyme and pathway databases

SABIO-RK Q29513.

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
InterProi IPR014369. Gly/Sar_N_MeTrfase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view ]
PANTHERi PTHR16458. PTHR16458. 1 hit.
PIRSFi PIRSF000385. Gly_N-mtase. 1 hit.
SUPFAMi SSF53335. SSF53335. 1 hit.
PROSITEi PS51600. SAM_GNMT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Mammalian glycine N-methyltransferases. Comparative kinetic and structural properties of the enzymes from human, rat, rabbit and pig livers."
    Ogawa H., Gomi T., Fujioka M.
    Comp. Biochem. Physiol. 106B:601-611(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Liver.

Entry informationi

Entry nameiGNMT_RABIT
AccessioniPrimary (citable) accession number: Q29513
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: October 29, 2014
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3