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Q29513

- GNMT_RABIT

UniProt

Q29513 - GNMT_RABIT

Protein

Glycine N-methyltransferase

Gene

GNMT

Organism
Oryctolagus cuniculus (Rabbit)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 79 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Catalyzes the methylation of glycine by using S-adenosylmethionine (AdoMet) to form N-methylglycine (sarcosine) with the concomitant production of S-adenosylhomocysteine (AdoHcy). Possible crucial role in the regulation of tissue concentration of AdoMet and of metabolism of methionine.

    Catalytic activityi

    S-adenosyl-L-methionine + glycine = S-adenosyl-L-homocysteine + sarcosine.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei20 – 201S-adenosyl-L-methioninePROSITE-ProRule annotation
    Binding sitei29 – 291S-adenosyl-L-methioninePROSITE-ProRule annotation
    Binding sitei32 – 321SubstratePROSITE-ProRule annotation
    Binding sitei39 – 391S-adenosyl-L-methioninePROSITE-ProRule annotation
    Binding sitei63 – 631S-adenosyl-L-methionine; via carbonyl oxygenPROSITE-ProRule annotation
    Binding sitei84 – 841S-adenosyl-L-methioninePROSITE-ProRule annotation
    Binding sitei137 – 1371S-adenosyl-L-methionine; via carbonyl oxygenPROSITE-ProRule annotation
    Binding sitei139 – 1391SubstratePROSITE-ProRule annotation
    Binding sitei176 – 1761SubstratePROSITE-ProRule annotation
    Binding sitei220 – 2201SubstratePROSITE-ProRule annotation

    GO - Molecular functioni

    1. folic acid binding Source: UniProtKB-KW
    2. glycine binding Source: UniProtKB
    3. glycine N-methyltransferase activity Source: UniProtKB

    GO - Biological processi

    1. S-adenosylmethionine metabolic process Source: UniProtKB

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Ligandi

    Folate-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    SABIO-RKQ29513.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glycine N-methyltransferase (EC:2.1.1.20)
    Gene namesi
    Name:GNMT
    OrganismiOryctolagus cuniculus (Rabbit)
    Taxonomic identifieri9986 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus
    ProteomesiUP000001811: Unplaced

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini‹1 – 292›292Glycine N-methyltransferasePRO_0000087527Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei8 – 81PhosphoserineBy similarity
    Modified residuei44 – 441N6-succinyllysineBy similarity
    Modified residuei190 – 1901N6-succinyllysineBy similarity
    Modified residuei195 – 1951N6-succinyllysineBy similarity
    Modified residuei200 – 2001N6-succinyllysineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Expressioni

    Tissue specificityi

    Abundant in liver.

    Interactioni

    Subunit structurei

    Homotetramer.

    Protein-protein interaction databases

    STRINGi9986.ENSOCUP00000003341.

    Structurei

    3D structure databases

    ProteinModelPortaliQ29513.
    SMRiQ29513. Positions 1-292.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni115 – 1162S-adenosyl-L-methionine bindingPROSITE-ProRule annotation

    Sequence similaritiesi

    Belongs to the class I-like SAM-binding methyltransferase superfamily. Glycine N-methyltransferase family.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG78825.
    HOGENOMiHOG000276537.
    HOVERGENiHBG051748.

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    InterProiIPR014369. Gly/Sar_N_MeTrfase.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view]
    PANTHERiPTHR16458. PTHR16458. 1 hit.
    PIRSFiPIRSF000385. Gly_N-mtase. 1 hit.
    SUPFAMiSSF53335. SSF53335. 1 hit.
    PROSITEiPS51600. SAM_GNMT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Fragment.

    Q29513-1 [UniParc]FASTAAdd to Basket

    « Hide

    DSVYRTRSLG VAAEGLPDQY ADGEAARVWQ LYIGDTRSRT SEYKTWLLGL    50
    LRQHGCRRVL DVACGTGVDS IMLVEEGFSV TSVDASDKML KYALKERWNR 100
    RHEPAFDKWV IEEANWMTLD KDVPQPAGGG FDAVICLGNS FAHLPDCKGD 150
    QSEHRQALKN IASMVRTGGL LVIDHRNYDH ILSTLCAPGK NIYYKSDLTK 200
    DVTTSVLTVN NKAHMVTLDY TVQVPAAGQD GPPSLSKFRL SYYPHCLAPF 250
    TEFLRAAFEG KCQHSVLGDF KPYQPGQAYV PCYFIHVLKR TA 292
    Length:292
    Mass (Da):32,626
    Last modified:November 1, 1996 - v1
    Checksum:iCF36EF0379313CDA
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Non-terminal residuei1 – 11

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D13307 mRNA. Translation: BAA02567.1.
    UniGeneiOcu.2139.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D13307 mRNA. Translation: BAA02567.1 .
    UniGenei Ocu.2139.

    3D structure databases

    ProteinModelPortali Q29513.
    SMRi Q29513. Positions 1-292.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9986.ENSOCUP00000003341.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Phylogenomic databases

    eggNOGi NOG78825.
    HOGENOMi HOG000276537.
    HOVERGENi HBG051748.

    Enzyme and pathway databases

    SABIO-RK Q29513.

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    InterProi IPR014369. Gly/Sar_N_MeTrfase.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view ]
    PANTHERi PTHR16458. PTHR16458. 1 hit.
    PIRSFi PIRSF000385. Gly_N-mtase. 1 hit.
    SUPFAMi SSF53335. SSF53335. 1 hit.
    PROSITEi PS51600. SAM_GNMT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Mammalian glycine N-methyltransferases. Comparative kinetic and structural properties of the enzymes from human, rat, rabbit and pig livers."
      Ogawa H., Gomi T., Fujioka M.
      Comp. Biochem. Physiol. 106B:601-611(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Liver.

    Entry informationi

    Entry nameiGNMT_RABIT
    AccessioniPrimary (citable) accession number: Q29513
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 79 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3