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Q29504 (UBA1_RABIT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Ubiquitin-like modifier-activating enzyme 1
Alternative name(s):
Ubiquitin-activating enzyme E1
Gene names
Name:UBA1
Synonyms:UBE1
OrganismOryctolagus cuniculus (Rabbit) [Reference proteome]
Taxonomic identifier9986 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Lagomorpha › Leporidae › Oryctolagus

Protein attributes

Sequence length1058 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding an ubiquitin-E1 thioester and free AMP.

Pathway

Protein modification; protein ubiquitination.

Subunit structure

Monomer By similarity.

Miscellaneous

There are two active sites within the E1 molecule, allowing it to accommodate two ubiquitin moieties at a time, with a new ubiquitin forming an adenylate intermediate as the previous one is transferred to the thiol site.

Sequence similarities

Belongs to the ubiquitin-activating E1 family.

Ontologies

Keywords
   Biological processUbl conjugation pathway
   DomainRepeat
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   PTMAcetylation
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processprotein ubiquitination

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

ligase activity

Inferred from electronic annotation. Source: UniProtKB-KW

small protein activating enzyme activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10581058Ubiquitin-like modifier-activating enzyme 1
PRO_0000194936

Regions

Repeat63 – 1991371-1
Repeat459 – 6111531-2
Nucleotide binding478 – 50730ATP By similarity
Region63 – 6115492 approximate repeats

Sites

Active site6321Glycyl thioester intermediate By similarity

Amino acid modifications

Modified residue131Phosphoserine By similarity
Modified residue211Phosphoserine By similarity
Modified residue241Phosphoserine By similarity
Modified residue461Phosphoserine By similarity
Modified residue551Phosphotyrosine By similarity
Modified residue6711N6-acetyllysine By similarity
Modified residue8001Phosphothreonine By similarity
Modified residue8101Phosphoserine By similarity
Modified residue8161Phosphoserine By similarity
Modified residue8201Phosphoserine By similarity
Modified residue8351Phosphoserine By similarity
Modified residue9801N6-acetyllysine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q29504 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 1196EAC5EEEC06A5

FASTA1,058117,688
        10         20         30         40         50         60 
MSSSPLSKKR RVSGPDPKPG SNCSPAQSVL PQVPSAPTNG MAKNGSEADI DEGLYSRQLY 

        70         80         90        100        110        120 
VLGHEAMKRL QTSSVLVSGL RGLGVEIAKN IILGGVKAVT LHDQGTAQWA DLSSQFYLRE 

       130        140        150        160        170        180 
EDIGKNRAEV SQPRLAELNS YVPVTAYTGP LVEDFLSGFQ VVVLTNSPLE DQLRVGEFCH 

       190        200        210        220        230        240 
SRGIKLVVAD TRGLFGQLFC DFGEEMILTD SNGEQPLSTM VSMVTKDNPG VVTCLDEARH 

       250        260        270        280        290        300 
GFESGDFVSF SEVQGMTELN GNQPIEIKVL GPYTFSICDT SNFSDYIRGG IVSQVKVPKK 

       310        320        330        340        350        360 
ISFKSLSASL AEPDFVMTDF AKFSRPAQLH IGFQALHKFC AQHSRPPRPR NEEDAAELVT 

       370        380        390        400        410        420 
LARAVNSKAS SAVQQDSLDE DLIRNLAFVA AGDLAPINAF IGGLAAQEVM KACSGKFMPI 

       430        440        450        460        470        480 
MQWLYFDALE CLPEDKESLT EDKCLPRQNR YDGQVAVFGS DLQEKLGRQK YFLVGAGAIG 

       490        500        510        520        530        540 
CELLKNFAMI GLGCGENGEI IVTDMDTIEK SNLNRQFLFR PWDVTKLKSD TAAAAVHQMN 

       550        560        570        580        590        600 
PHIRVTSHQN RVGPDTERIY DDDFFQTLDG VANALDNVDA RMYMDRRCVY YRKPLLESGT 

       610        620        630        640        650        660 
LGTKGNVQVV IPFLTESYSS SQDPPEKSIP ICTLKNFPNA IEHTLQWARD EFEGLFKQPA 

       670        680        690        700        710        720 
ENVNQYLTDP KFVERTLRLA GTQPLEVLEA VQRSLVLQLP QSWADCVTWA CHHWHTQYSN 

       730        740        750        760        770        780 
NIRQLLHNFP PDQLTSSGAP FWSGPKRCPH PLTFDVSNPL HLDYVMAAAN LFAQTYGLAG 

       790        800        810        820        830        840 
SQDRAAVATL LQSVQVPEFT PKSGVKIHVS DQELQSANAS VDDSRLEELK ATLPSPDKLP 

       850        860        870        880        890        900 
GFKMYPIDFE KDDDSNFHMD FIVAASNLRA ENYDIPPADR HKSKLIAGKI IPAIATTTAA 

       910        920        930        940        950        960 
VVGLVCLELY KVVQGHRHLD SYKNGFLNLA LPFFGFSEPL AAPRHQYYNQ EWTLWDRFEV 

       970        980        990       1000       1010       1020 
QGLQPNGEEM TLKQFLDYFK TEHKLEITML SQGVSMLYSF FMPAAKLKER LDQPMTEIVS 

      1030       1040       1050 
RVSKRKLGRH VRALVLELCC NDESGEDVEV PYVRYTIR

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References

[1]"Rabbit ubiquitin-activating enzyme E1: cDNA cloning, sequence and expression."
Sun B., Jeyaseelan K., Chung M.C.M., Teo T.S.
Gene 196:19-23(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Heart.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		U58653 mRNA. Translation: AAC48768.1.
RefSeqNP_001075840.1. NM_001082371.2.
UniGeneOcu.6207.

3D structure databases

ProteinModelPortalQ29504.
SMRQ29504. Positions 48-1057.
ModBaseSearch...

Protein-protein interaction databases

IntActQ29504. 7 interactions.
STRING9986.ENSOCUP00000007237.

Proteomic databases

PRIDEQ29504.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID100009225.

Organism-specific databases

CTD7317.

Phylogenomic databases

eggNOGCOG0476.
HOGENOMHOG000167329.
HOVERGENHBG054199.
OrthoDBEOG4QZ7K4.

Enzyme and pathway databases

UniPathwayUPA00143.

Family and domain databases

Gene3D1.10.3240.10. 1 hit.
3.40.50.720. 4 hits.
InterProIPR009036. Molybdenum_cofac_synth_MoeB.
IPR016040. NAD(P)-bd_dom.
IPR000594. ThiF_NAD_FAD-bd.
IPR018965. Ub-activating_enz_e1_C.
IPR023280. Ub-like_act_enz_cat_cys_dom.
IPR000127. UBact_repeat.
IPR019572. Ubiquitin-activating_enzyme.
IPR018075. UBQ-activ_enz_E1.
IPR018074. UBQ-activ_enz_E1_AS.
IPR000011. UBQ/SUMO-activ_enz_E1-like.
[Graphical view]
PfamPF00899. ThiF. 2 hits.
PF09358. UBA_e1_C. 1 hit.
PF10585. UBA_e1_thiolCys. 1 hit.
PF02134. UBACT. 2 hits.
[Graphical view]
PRINTSPR01849. UBIQUITINACT.
SMARTSM00985. UBA_e1_C. 1 hit.
[Graphical view]
SUPFAMSSF69572. MoeB. 2 hits.
TIGRFAMsTIGR01408. Ube1. 1 hit.
PROSITEPS00536. UBIQUITIN_ACTIVAT_1. 1 hit.
PS00865. UBIQUITIN_ACTIVAT_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameUBA1_RABIT
AccessionPrimary (citable) accession number: Q29504
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: November 1, 1996
Last modified: April 3, 2013
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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