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Q29498 (NOS1_SHEEP) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 105. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Nitric oxide synthase, brain

EC=1.14.13.39
Alternative name(s):
BNOS
Constitutive NOS
NC-NOS
NOS type I
Neuronal NOS
Short name=N-NOS
Short name=NNOS
Peptidyl-cysteine S-nitrosylase NOS1
Gene names
Name:NOS1
OrganismOvis aries (Sheep) [Complete proteome]
Taxonomic identifier9940 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeCaprinaeOvis

Protein attributes

Sequence length186 AA.
Sequence statusFragments.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR By similarity.

Catalytic activity

2 L-arginine + 3 NADPH + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O.

Cofactor

Heme group By similarity.

Binds 1 FAD By similarity.

Binds 1 FMN By similarity.

Tetrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme By similarity.

Enzyme regulation

Stimulated by calcium/calmodulin. Inhibited by n-Nos-inhibiting protein (PIN) which may prevent the dimerization of the protein. Inhibited by NOSIP By similarity.

Subunit structure

Homodimer. Interacts with DLG4; the interaction possibly being prevented by the association between NOS1 and CAPON. Forms a ternary complex with CAPON and RASD1. Forms a ternary complex with CAPON and SYN1. Interacts with ZDHHC23. Interacts with NOSIP; which may impair its synaptic location By similarity. Interacts with HTR4 By similarity. Interacts with SLC6A4 By similarity. Interacts (via N-terminus domain) with DLG4 (via N-terminus tandem pair of PDZ domains) By similarity.

Subcellular location

Cell membranesarcolemma; Peripheral membrane protein By similarity. Cell projectiondendritic spine By similarity. Note: In skeletal muscle, NNOS is localized beneath the sarcolemma of fast-twitch muscle fiber by associating with the dystrophin glycoprotein complex. In neurons, enriched in dendritic spines By similarity.

Post-translational modification

Ubiquitinated; mediated by STUB1/CHIP in the presence of Hsp70 and Hsp40 (in vitro) By similarity.

Sequence similarities

Belongs to the NOS family.

Contains 1 PDZ (DHR) domain.

Ontologies

Keywords
   Cellular componentCell membrane
Cell projection
Membrane
   LigandCalmodulin-binding
FAD
Flavoprotein
FMN
Heme
Iron
Metal-binding
NADP
   Molecular functionOxidoreductase
   PTMUbl conjugation
   Technical termComplete proteome
Gene Ontology (GO)
   Cellular_componentdendritic spine

Inferred from electronic annotation. Source: UniProtKB-SubCell

sarcolemma

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionmetal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

nitric-oxide synthase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain‹1 – ›186›186Nitric oxide synthase, brain
PRO_0000170925

Regions

Domain‹1 – 51›51PDZ
Nucleotide binding115 – 12612FAD By similarity

Experimental info

Non-adjacent residues73 – 742
Non-terminal residue11
Non-terminal residue1861

Sequences

Sequence LengthMass (Da)Tools
Q29498 [UniParc].

Last modified May 1, 1999. Version 2.
Checksum: 021B7925AE35CB97

FASTA18620,486
        10         20         30         40         50         60 
GGAAEQSGLI QAGDIILAVN GQPLVDLSYD SALEVFRGIA SETHVVLILR GPEGFTTHLE 

        70         80         90        100        110        120 
TTFTGDGTPK TIHNVHKKRV SAARLLSRQN LQSPKSSRST IFVRLHTNGN QELQYQPGDH 

       130        140        150        160        170        180 
LGVFPGNHED LVNALIERLE DAPPANQLVK VELLEERNTA LGVISNWTDE HRLPPCTIFQ 


AFKYYL 

« Hide

References

[1]Mimmack M.L.
Submitted (JUL-1996) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-73.
[2]"Effect of hypoxia on the expression pattern of neuronal nitric oxide synthase gene in the sheep fetal brain microvasculature."
Aguan K., Weiner C.P.
Submitted (OCT-1996) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 74-186.
Tissue: Neuron.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X99042 Genomic DNA. Translation: CAA67503.1.
U76739 mRNA. Translation: AAB40706.1.
UniGeneOar.522.

3D structure databases

ProteinModelPortalQ29498.
SMRQ29498. Positions 1-186.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D1.20.990.10. 1 hit.
2.30.42.10. 1 hit.
InterProIPR003097. FAD-binding_1.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR001478. PDZ.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamPF00667. FAD_binding_1. 1 hit.
PF00595. PDZ. 1 hit.
[Graphical view]
SMARTSM00228. PDZ. 1 hit.
[Graphical view]
SUPFAMSSF50156. SSF50156. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEPS50106. PDZ. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNOS1_SHEEP
AccessionPrimary (citable) accession number: Q29498
Secondary accession number(s): P79210
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 1, 1999
Last modified: April 16, 2014
This is version 105 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families