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Q29498

- NOS1_SHEEP

UniProt

Q29498 - NOS1_SHEEP

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Protein
Nitric oxide synthase, brain
Gene
NOS1
Organism
Ovis aries (Sheep)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR By similarity.

Catalytic activityi

2 L-arginine + 3 NADPH + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O.

Cofactori

Heme group By similarity.
Binds 1 FAD By similarity.
Binds 1 FMN By similarity.
Tetrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme By similarity.

Enzyme regulationi

Stimulated by calcium/calmodulin. Inhibited by n-Nos-inhibiting protein (PIN) which may prevent the dimerization of the protein. Inhibited by NOSIP By similarity.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi115 – 12612FAD By similarity
Add
BLAST

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. nitric-oxide synthase activity Source: UniProtKB-EC
Complete GO annotation...

GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    Calmodulin-binding, FAD, Flavoprotein, FMN, Heme, Iron, Metal-binding, NADP

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Nitric oxide synthase, brain (EC:1.14.13.39)
    Alternative name(s):
    BNOS
    Constitutive NOS
    NC-NOS
    NOS type I
    Neuronal NOS
    Short name:
    N-NOS
    Short name:
    NNOS
    Peptidyl-cysteine S-nitrosylase NOS1
    Gene namesi
    Name:NOS1
    OrganismiOvis aries (Sheep)
    Taxonomic identifieri9940 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeCaprinaeOvis
    ProteomesiUP000002356: Unplaced

    Subcellular locationi

    Cell membranesarcolemma; Peripheral membrane protein By similarity. Cell projectiondendritic spine By similarity
    Note: In skeletal muscle, NNOS is localized beneath the sarcolemma of fast-twitch muscle fiber by associating with the dystrophin glycoprotein complex. In neurons, enriched in dendritic spines By similarity.

    GO - Cellular componenti

    1. dendritic spine Source: UniProtKB-SubCell
    2. sarcolemma Source: UniProtKB-SubCell
    Complete GO annotation...

    Keywords - Cellular componenti

    Cell membrane, Cell projection, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini‹1 – ›186›186Nitric oxide synthase, brain
    PRO_0000170925Add
    BLAST

    Post-translational modificationi

    Ubiquitinated; mediated by STUB1/CHIP in the presence of Hsp70 and Hsp40 (in vitro) By similarity.

    Keywords - PTMi

    Ubl conjugation

    Interactioni

    Subunit structurei

    Homodimer. Interacts with DLG4; the interaction possibly being prevented by the association between NOS1 and CAPON. Forms a ternary complex with CAPON and RASD1. Forms a ternary complex with CAPON and SYN1. Interacts with ZDHHC23. Interacts with NOSIP; which may impair its synaptic location By similarity. Interacts with HTR4 By similarity. Interacts with SLC6A4 By similarity. Interacts (via N-terminal domain) with DLG4 (via N-terminal tandem pair of PDZ domains) By similarity.

    Structurei

    3D structure databases

    ProteinModelPortaliQ29498.
    SMRiQ29498. Positions 1-186.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini‹1 – 51›51PDZ
    Add
    BLAST

    Sequence similaritiesi

    Belongs to the NOS family.
    Contains 1 PDZ (DHR) domain.

    Family and domain databases

    Gene3Di1.20.990.10. 1 hit.
    2.30.42.10. 1 hit.
    InterProiIPR003097. FAD-binding_1.
    IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
    IPR001478. PDZ.
    IPR017938. Riboflavin_synthase-like_b-brl.
    [Graphical view]
    PfamiPF00667. FAD_binding_1. 1 hit.
    PF00595. PDZ. 1 hit.
    [Graphical view]
    SMARTiSM00228. PDZ. 1 hit.
    [Graphical view]
    SUPFAMiSSF50156. SSF50156. 1 hit.
    SSF63380. SSF63380. 1 hit.
    PROSITEiPS50106. PDZ. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Fragments.

    Q29498-1 [UniParc]FASTAAdd to Basket

    « Hide

    GGAAEQSGLI QAGDIILAVN GQPLVDLSYD SALEVFRGIA SETHVVLILR    50
    GPEGFTTHLE TTFTGDGTPK TIHNVHKKRV SAARLLSRQN LQSPKSSRST 100
    IFVRLHTNGN QELQYQPGDH LGVFPGNHED LVNALIERLE DAPPANQLVK 150
    VELLEERNTA LGVISNWTDE HRLPPCTIFQ AFKYYL 186
    Length:186
    Mass (Da):20,486
    Last modified:May 1, 1999 - v2
    Checksum:i021B7925AE35CB97
    GO

    Non-adjacent residues

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Non-adjacent residuesi73 – 742

    Non-terminal residue

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Non-terminal residuei1 – 11
    Non-terminal residuei186 – 1861

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X99042 Genomic DNA. Translation: CAA67503.1.
    U76739 mRNA. Translation: AAB40706.1.
    UniGeneiOar.522.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X99042 Genomic DNA. Translation: CAA67503.1 .
    U76739 mRNA. Translation: AAB40706.1 .
    UniGenei Oar.522.

    3D structure databases

    ProteinModelPortali Q29498.
    SMRi Q29498. Positions 1-186.
    ModBasei Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 1.20.990.10. 1 hit.
    2.30.42.10. 1 hit.
    InterProi IPR003097. FAD-binding_1.
    IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
    IPR001478. PDZ.
    IPR017938. Riboflavin_synthase-like_b-brl.
    [Graphical view ]
    Pfami PF00667. FAD_binding_1. 1 hit.
    PF00595. PDZ. 1 hit.
    [Graphical view ]
    SMARTi SM00228. PDZ. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50156. SSF50156. 1 hit.
    SSF63380. SSF63380. 1 hit.
    PROSITEi PS50106. PDZ. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Mimmack M.L.
      Submitted (JUL-1996) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-73.
    2. "Effect of hypoxia on the expression pattern of neuronal nitric oxide synthase gene in the sheep fetal brain microvasculature."
      Aguan K., Weiner C.P.
      Submitted (OCT-1996) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 74-186.
      Tissue: Neuron.

    Entry informationi

    Entry nameiNOS1_SHEEP
    AccessioniPrimary (citable) accession number: Q29498
    Secondary accession number(s): P79210
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: May 1, 1999
    Last modified: July 9, 2014
    This is version 107 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

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