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Protein

Nitric oxide synthase, brain

Gene

NOS1

Organism
Ovis aries (Sheep)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR (By similarity).By similarity

Catalytic activityi

2 L-arginine + 3 NADPH + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O.

Cofactori

Protein has several cofactor binding sites:

Enzyme regulationi

Stimulated by calcium/calmodulin. Inhibited by n-Nos-inhibiting protein (PIN) which may prevent the dimerization of the protein. Inhibited by NOSIP (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi115 – 126FADBy similarityAdd BLAST12

GO - Molecular functioni

Keywordsi

Molecular functionCalmodulin-binding, Oxidoreductase
LigandFAD, Flavoprotein, FMN, Heme, Iron, Metal-binding, NADP

Names & Taxonomyi

Protein namesi
Recommended name:
Nitric oxide synthase, brain (EC:1.14.13.39)
Alternative name(s):
BNOS
Constitutive NOS
NC-NOS
NOS type I
Neuronal NOS
Short name:
N-NOS
Short name:
NNOS
Peptidyl-cysteine S-nitrosylase NOS1
Gene namesi
Name:NOS1
OrganismiOvis aries (Sheep)
Taxonomic identifieri9940 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeCaprinaeOvis
Proteomesi
  • UP000002356 Componenti: Unplaced

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cell projection, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000170925‹1 – ›186Nitric oxide synthase, brainAdd BLAST›186

Post-translational modificationi

Ubiquitinated; mediated by STUB1/CHIP in the presence of Hsp70 and Hsp40 (in vitro).By similarity

Keywords - PTMi

Ubl conjugation

Interactioni

Subunit structurei

Homodimer. Interacts with DLG4; the interaction possibly being prevented by the association between NOS1 and CAPON. Forms a ternary complex with CAPON and RASD1. Forms a ternary complex with CAPON and SYN1. Interacts with ZDHHC23. Interacts with NOSIP; which may impair its synaptic location (By similarity). Interacts with HTR4 (By similarity). Interacts with SLC6A4 (By similarity). Interacts (via N-terminal domain) with DLG4 (via N-terminal tandem pair of PDZ domains) (By similarity).By similarity

GO - Molecular functioni

Structurei

3D structure databases

ProteinModelPortaliQ29498.
SMRiQ29498.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini‹1 – 51PDZPROSITE-ProRule annotationAdd BLAST›51

Sequence similaritiesi

Belongs to the NOS family.Curated

Family and domain databases

InterProiView protein in InterPro
IPR003097. FAD-binding_1.
IPR001478. PDZ.
IPR036034. PDZ_sf.
IPR017938. Riboflavin_synthase-like_b-brl.
PfamiView protein in Pfam
PF00667. FAD_binding_1. 1 hit.
PF00595. PDZ. 1 hit.
SUPFAMiSSF50156. SSF50156. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEiView protein in PROSITE
PS50106. PDZ. 1 hit.

Sequencei

Sequence statusi: Fragments.

Q29498-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
GGAAEQSGLI QAGDIILAVN GQPLVDLSYD SALEVFRGIA SETHVVLILR
60 70 80 90 100
GPEGFTTHLE TTFTGDGTPK TIHNVHKKRV SAARLLSRQN LQSPKSSRST
110 120 130 140 150
IFVRLHTNGN QELQYQPGDH LGVFPGNHED LVNALIERLE DAPPANQLVK
160 170 180
VELLEERNTA LGVISNWTDE HRLPPCTIFQ AFKYYL
Length:186
Mass (Da):20,486
Last modified:May 1, 1999 - v2
Checksum:i021B7925AE35CB97
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Non-terminal residuei11
Non-adjacent residuesi73 – 74Curated2
Non-terminal residuei1861

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X99042 Genomic DNA. Translation: CAA67503.1.
U76739 mRNA. Translation: AAB40706.1.
UniGeneiOar.522.

Entry informationi

Entry nameiNOS1_SHEEP
AccessioniPrimary (citable) accession number: Q29498
Secondary accession number(s): P79210
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 1, 1999
Last modified: October 25, 2017
This is version 120 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families