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Reviewed, UniProtKB/Swiss-Prot Q29498 (NOS1_SHEEP)

Last modified June 16, 2009. Version 78. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Nitric oxide synthase, brain
    EC=1.14.13.39
Alternative name(s):
    BNOS
    NOS type I
    Neuronal NOS
      Short name=N-NOS
      Short name=NNOS
    Constitutive NOS
    NC-NOS
Gene names
Name: NOS1
OrganismOvis aries (Sheep)
Taxonomic identifier9940 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeCaprinaeOvis

Protein attributes

Sequence length186 AA.
Sequence statusFragments.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at transcript level.

General annotation (Comments)

Function

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter.

Catalytic activity

L-arginine + n NADPH + n H+ + m O2 = citrulline + nitric oxide + n NADP+.

Cofactor

Heme group By similarity.

Binds 1 FAD By similarity.

Binds 1 FMN By similarity.

Tetrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme By similarity.

Enzyme regulation

Stimulated by calcium/calmodulin. Inhibited by n-Nos-inhibiting protein (PIN) which may prevent the dimerization of the protein. Inhibited by NOSIP By similarity.

Subunit structure

Homodimer. Interacts with DLG4; the interaction possibly being prevented by the association between NOS1 and CAPON. Forms a ternary complex with CAPON and RASD1. Forms a ternary complex with CAPON and SYN1. Interacts with ZDHHC23. Interacts with NOSIP; which may impair its synaptic location By similarity. Interacts with HTR4 By similarity.

Subcellular location

Cell membranesarcolemma; Peripheral membrane protein By similarity. Cell projectiondendritic spine By similarity. Note: In skeletal muscle, NNOS is localized beneath the sarcolemma of fast-twitch muscle fiber by associating with the dystrophin glycoprotein complex. In neurons, enriched in dendritic spines By similarity.

Sequence similarities

Belongs to the NOS family.

Contains 1 PDZ (DHR) domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain‹1 – ›186›186Nitric oxide synthase, brain
PRO_0000170925

Regions

Domain‹1 – 51›51PDZ
Nucleotide binding115 – 12612FAD By similarity

Experimental info

Non-adjacent residues73 – 742
Non-terminal residue11
Non-terminal residue1861

Sequences

Sequence LengthMass (Da)Tools
Q29498-1 [UniParc].

Last modified May 1, 1999. Version 2.
Checksum: 021B7925AE35CB97

FASTA18620,486
        10         20         30         40         50         60 
GGAAEQSGLI QAGDIILAVN GQPLVDLSYD SALEVFRGIA SETHVVLILR GPEGFTTHLE 

        70         80         90        100        110        120 
TTFTGDGTPK TIHNVHKKRV SAARLLSRQN LQSPKSSRST IFVRLHTNGN QELQYQPGDH 

       130        140        150        160        170        180 
LGVFPGNHED LVNALIERLE DAPPANQLVK VELLEERNTA LGVISNWTDE HRLPPCTIFQ 


AFKYYL 

« Hide

References

[1]Mimmack M.L.
Submitted (JUL-1996) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE OF 1-73.
[2]"Effect of hypoxia on the expression pattern of neuronal nitric oxide synthase gene in the sheep fetal brain microvasculature."
Aguan K., Weiner C.P.
Submitted (OCT-1996) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE OF 74-186.
Tissue: Neuron.

Cross-references

Sequence databases

X99042 Genomic DNA. Translation: CAA67503.1.
U76739 mRNA. Translation: AAB40706.1.
UniGeneOar.522

3D structure databases

HSSPHSSP built from PDB template 1QAU based on UniProtKB P29476.
SMRQ29498. Positions 1-72, 72-186.
ModBaseSearch...

Phylogenomic databases

HOVERGENQ29498.

Enzyme and pathway databases

BRENDA1.14.13.39. 271.

Family and domain databases

InterProIPR003097. FAD-binding_1.
IPR004030. NO_synthase_oxygenase_reg.
IPR001478. PDZ/DHR/GLGF.
[Graphical view]
PfamPF00667. FAD_binding_1. 1 hit.
PF00595. PDZ. 1 hit.
[Graphical view]
SMARTSM00228. PDZ. 1 hit.
[Graphical view]
PROSITEPS60001. NOS. Partial match.
PS50106. PDZ. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNOS1_SHEEP
AccessionPrimary (citable) accession number: Q29498
Secondary accession number(s): P79210
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 1, 1999
Last modified: June 16, 2009
This is version 78 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents