Q29498 (NOS1_SHEEP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nitric oxide synthase, brain
EC=1.14.13.39

Alternative name(s):
BNOS
Constitutive NOS
NC-NOS
NOS type I
Neuronal NOS
Short name=N-NOS
Short name=NNOS
Peptidyl-cysteine S-nitrosylase NOS1

Gene names

Name:

NOS1

Organism

Ovis aries (Sheep)

Taxonomic identifier

9940 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Caprinae › Ovis

Protein attributes

Sequence length	186 AA.
Sequence status	Fragments.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR By similarity.
Catalytic activity	2 L-arginine + 3 NADPH + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O.
Cofactor	Heme group By similarity. Binds 1 FAD By similarity. Binds 1 FMN By similarity. Tetrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme By similarity.
Enzyme regulation	Stimulated by calcium/calmodulin. Inhibited by n-Nos-inhibiting protein (PIN) which may prevent the dimerization of the protein. Inhibited by NOSIP By similarity.
Subunit structure	Homodimer. Interacts with DLG4; the interaction possibly being prevented by the association between NOS1 and CAPON. Forms a ternary complex with CAPON and RASD1. Forms a ternary complex with CAPON and SYN1. Interacts with ZDHHC23. Interacts with NOSIP; which may impair its synaptic location By similarity. Interacts with HTR4 By similarity. Interacts with SLC6A4 By similarity.
Subcellular location	Cell membrane › sarcolemma; Peripheral membrane protein By similarity. Cell projection › dendritic spine By similarity. Note: In skeletal muscle, NNOS is localized beneath the sarcolemma of fast-twitch muscle fiber by associating with the dystrophin glycoprotein complex. In neurons, enriched in dendritic spines By similarity.
Post-translational modification	Ubiquitinated; mediated by STUB1/CHIP in the presence of Hsp70 and Hsp40 (in vitro) By similarity.
Sequence similarities	Belongs to the NOS family. Contains 1 PDZ (DHR) domain.

Ontologies

Keywords
Cellular component	Cell membrane Cell projection Membrane
Ligand	Calmodulin-binding FAD FMN Heme Iron Metal-binding NADP
Molecular function	Oxidoreductase
PTM	Ubl conjugation
Gene Ontology (GO)
Biological_process	nitric oxide biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular_component	dendritic spine Inferred from electronic annotation. Source: UniProtKB-SubCell sarcolemma Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nitric-oxide synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – ›186	›186	Nitric oxide synthase, brain		PRO_0000170925
Regions
	Domain	‹1 – 51	›51	PDZ
	Nucleotide binding	115 – 126	12	FAD By similarity
Experimental info
	Non-adjacent residues	73 – 74	2
	Non-terminal residue	1	1
	Non-terminal residue	186	1

Sequences

	Sequence		Length	Mass (Da)
	Q29498 [UniParc]. Last modified May 1, 1999. Version 2. Checksum: 021B7925AE35CB97	FASTA	186	20,486
	10 20 30 40 50 60 GGAAEQSGLI QAGDIILAVN GQPLVDLSYD SALEVFRGIA SETHVVLILR GPEGFTTHLE 70 80 90 100 110 120 TTFTGDGTPK TIHNVHKKRV SAARLLSRQN LQSPKSSRST IFVRLHTNGN QELQYQPGDH 130 140 150 160 170 180 LGVFPGNHED LVNALIERLE DAPPANQLVK VELLEERNTA LGVISNWTDE HRLPPCTIFQ AFKYYL

References

[1]	Mimmack M.L. Submitted (JUL-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-73.
[2]	"Effect of hypoxia on the expression pattern of neuronal nitric oxide synthase gene in the sheep fetal brain microvasculature." Aguan K., Weiner C.P. Submitted (OCT-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 74-186. Tissue: Neuron.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X99042 Genomic DNA. Translation: CAA67503.1. U76739 mRNA. Translation: AAB40706.1.
UniGene	Oar.522.
3D structure databases
ProteinModelPortal	Q29498.
SMR	Q29498. Positions 1-186.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	1.20.990.10. 1 hit.
InterPro	IPR003097. FAD-binding_1. IPR023173. NADPH_Cyt_P450_Rdtase_dom3. IPR026009. NOS. IPR001478. PDZ. IPR017938. Riboflavin_synthase-like_b-brl. [Graphical view]
PANTHER	PTHR19384:SF5. PTHR19384:SF5. 1 hit.
Pfam	PF00667. FAD_binding_1. 1 hit. PF00595. PDZ. 1 hit. [Graphical view]
SMART	SM00228. PDZ. 1 hit. [Graphical view]
SUPFAM	SSF50156. PDZ. 1 hit. SSF63380. Riboflavin_synthase_like_b-brl. 1 hit.
PROSITE	PS60001. NOS. Partial match. PS50106. PDZ. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NOS1_SHEEP

Accession

Primary (citable) accession number: Q29498
Secondary accession number(s): P79210

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 1, 1999
Last modified:	March 6, 2013
This is version 99 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program