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Q29497

- CP17A_SHEEP

UniProt

Q29497 - CP17A_SHEEP

Protein

Steroid 17-alpha-hydroxylase/17,20 lyase

Gene

CYP17A1

Organism
Ovis aries (Sheep)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 91 (01 Oct 2014)
      Sequence version 2 (04 Jan 2005)
      Previous versions | rss
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    Functioni

    Conversion of pregnenolone and progesterone to their 17-alpha-hydroxylated products and subsequently to dehydroepiandrosterone (DHEA) and androstenedione. Catalyzes both the 17-alpha-hydroxylation and the 17,20-lyase reaction. Involved in sexual development during fetal life and at puberty.

    Catalytic activityi

    A C(21)-steroid + (reduced NADPH--hemoprotein reductase) + O2 = a 17-alpha-hydroxy-C(21)-steroid + (oxidized NADPH--hemoprotein reductase) + H2O.
    17-alpha-hydroxyprogesterone = androst-4-ene-3,17-dione + acetaldehyde.

    Cofactori

    Heme group.By similarity

    Enzyme regulationi

    Regulated predominantly by intracellular cAMP levels.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi442 – 4421Iron (heme axial ligand)By similarity

    GO - Molecular functioni

    1. 17-alpha-hydroxyprogesterone aldolase activity Source: UniProtKB-EC
    2. heme binding Source: UniProtKB
    3. iron ion binding Source: InterPro
    4. steroid 17-alpha-monooxygenase activity Source: UniProtKB

    GO - Biological processi

    1. hormone biosynthetic process Source: UniProtKB
    2. progesterone metabolic process Source: UniProtKB
    3. steroid biosynthetic process Source: UniProtKB-UniPathway
    4. steroid metabolic process Source: UniProtKB

    Keywords - Molecular functioni

    Lyase, Monooxygenase, Oxidoreductase

    Keywords - Biological processi

    Steroidogenesis

    Keywords - Ligandi

    Heme, Iron, Metal-binding

    Enzyme and pathway databases

    UniPathwayiUPA00062.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Steroid 17-alpha-hydroxylase/17,20 lyase (EC:1.14.99.9, EC:4.1.2.30)
    Alternative name(s):
    17-alpha-hydroxyprogesterone aldolase
    CYPXVII
    Cytochrome P450 17A1
    Cytochrome P450-C17
    Short name:
    Cytochrome P450c17
    Gene namesi
    Name:CYP17A1
    Synonyms:CYP17
    OrganismiOvis aries (Sheep)
    Taxonomic identifieri9940 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeCaprinaeOvis
    ProteomesiUP000002356: Unplaced

    Subcellular locationi

    Membrane Curated

    GO - Cellular componenti

    1. membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 509509Steroid 17-alpha-hydroxylase/17,20 lyasePRO_0000051942Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliQ29497.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the cytochrome P450 family.Curated

    Phylogenomic databases

    HOVERGENiHBG106944.

    Family and domain databases

    Gene3Di1.10.630.10. 1 hit.
    InterProiIPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    [Graphical view]
    PfamiPF00067. p450. 1 hit.
    [Graphical view]
    PRINTSiPR00463. EP450I.
    PR00385. P450.
    SUPFAMiSSF48264. SSF48264. 1 hit.
    PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q29497-1 [UniParc]FASTAAdd to Basket

    « Hide

    MWVLLAVFLL TLAYLFWPKT KHSGAKYPRS LPSLPLVGSL PFLPRRGQQH    50
    ENFFKLQEKY GPIYSFRLGS KTTVMIGHHQ LAREVLLKKG KEFSGRPKVA 100
    TLDILSDNQK GIAFADHGAH WQLHRKLVLN AFALFKDGNL KLEKIINQEA 150
    NVLCDFLATQ HGQSIDLSEP LSLAVTNIIS FICFNFSFKN EDPALKAIQN 200
    VNDGILEVLG KEVLLDIFPA LKIFPSKAME KMKGCVETRN ELLSEILEKC 250
    QENFTSDSIT NLLHILMQAK VNADNNNTGP EQDSKLLSNR HMLATIADIF 300
    GAGVETTTSV IKWIVAYLLH HPSLKKRIQD SIDQNIGFNR TPTISDRNRL 350
    VLLEATIREV LRIRPVAPML IPHKAIIDSS IGDLTIDKGT DVVVNLWALH 400
    HNEKEWQQPD LFMPERFLDP TGTQLISPSL SYLPFGAGPR SCVGEMLARQ 450
    ELFLFMSRLL QRFNLEIPDD GKLPSLEGNP SLVLQIKPFK VKIEVRQAWK 500
    EAQAEGSTS 509
    Length:509
    Mass (Da):57,308
    Last modified:January 4, 2005 - v2
    Checksum:iD498E639D36A5148
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti210 – 2101G → S in AAA63517. 1 PublicationCurated
    Sequence conflicti464 – 4641N → Y in AAA63517. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L40335 mRNA. Translation: AAA63517.1.
    AF251388 mRNA. Translation: AAF65824.1.
    RefSeqiNP_001009483.1. NM_001009483.1.
    UniGeneiOar.399.

    Genome annotation databases

    GeneIDi493968.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L40335 mRNA. Translation: AAA63517.1 .
    AF251388 mRNA. Translation: AAF65824.1 .
    RefSeqi NP_001009483.1. NM_001009483.1.
    UniGenei Oar.399.

    3D structure databases

    ProteinModelPortali Q29497.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 493968.

    Organism-specific databases

    CTDi 1586.

    Phylogenomic databases

    HOVERGENi HBG106944.

    Enzyme and pathway databases

    UniPathwayi UPA00062 .

    Family and domain databases

    Gene3Di 1.10.630.10. 1 hit.
    InterProi IPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    [Graphical view ]
    Pfami PF00067. p450. 1 hit.
    [Graphical view ]
    PRINTSi PR00463. EP450I.
    PR00385. P450.
    SUPFAMi SSF48264. SSF48264. 1 hit.
    PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and expression of ovine cytochrome P-450 17-alpha hydroxylase/c17-20 lyase."
      Murry B.A., Swart P., Mason J.I.
      Submitted (MAR-1995) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Adrenal cortex.
    2. "Molecular cloning and heterologous expression in E. coli of cytochrome P45017alpha. Comparison of structural and functional properties of substrate-specific cytochromes P450 from different species."
      Gilep A.A., Estabrook R.W., Usanov S.A.
      Biochemistry (Mosc.) 68:86-98(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Adrenal gland.

    Entry informationi

    Entry nameiCP17A_SHEEP
    AccessioniPrimary (citable) accession number: Q29497
    Secondary accession number(s): Q9N0U6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: January 4, 2005
    Last modified: October 1, 2014
    This is version 91 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3