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Q29497

- CP17A_SHEEP

UniProt

Q29497 - CP17A_SHEEP

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Protein
Steroid 17-alpha-hydroxylase/17,20 lyase
Gene
CYP17A1, CYP17
Organism
Ovis aries (Sheep)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Conversion of pregnenolone and progesterone to their 17-alpha-hydroxylated products and subsequently to dehydroepiandrosterone (DHEA) and androstenedione. Catalyzes both the 17-alpha-hydroxylation and the 17,20-lyase reaction. Involved in sexual development during fetal life and at puberty.

Catalytic activityi

A C(21)-steroid + (reduced NADPH--hemoprotein reductase) + O2 = a 17-alpha-hydroxy-C(21)-steroid + (oxidized NADPH--hemoprotein reductase) + H2O.
17-alpha-hydroxyprogesterone = androst-4-ene-3,17-dione + acetaldehyde.

Cofactori

Heme group By similarity.

Enzyme regulationi

Regulated predominantly by intracellular cAMP levels By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi442 – 4421Iron (heme axial ligand) By similarity

GO - Molecular functioni

  1. 17-alpha-hydroxyprogesterone aldolase activity Source: UniProtKB-EC
  2. heme binding Source: UniProtKB
  3. iron ion binding Source: InterPro
  4. steroid 17-alpha-monooxygenase activity Source: UniProtKB

GO - Biological processi

  1. hormone biosynthetic process Source: UniProtKB
  2. progesterone metabolic process Source: UniProtKB
  3. steroid biosynthetic process Source: UniProtKB-UniPathway
  4. steroid metabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Lyase, Monooxygenase, Oxidoreductase

Keywords - Biological processi

Steroidogenesis

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00062.

Names & Taxonomyi

Protein namesi
Recommended name:
Steroid 17-alpha-hydroxylase/17,20 lyase (EC:1.14.99.9, EC:4.1.2.30)
Alternative name(s):
17-alpha-hydroxyprogesterone aldolase
CYPXVII
Cytochrome P450 17A1
Cytochrome P450-C17
Short name:
Cytochrome P450c17
Gene namesi
Name:CYP17A1
Synonyms:CYP17
OrganismiOvis aries (Sheep)
Taxonomic identifieri9940 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeCaprinaeOvis
ProteomesiUP000002356: Unplaced

Subcellular locationi

Membrane Reviewed prediction

GO - Cellular componenti

  1. membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 509509Steroid 17-alpha-hydroxylase/17,20 lyase
PRO_0000051942Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ29497.

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.

Phylogenomic databases

HOVERGENiHBG106944.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q29497-1 [UniParc]FASTAAdd to Basket

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MWVLLAVFLL TLAYLFWPKT KHSGAKYPRS LPSLPLVGSL PFLPRRGQQH    50
ENFFKLQEKY GPIYSFRLGS KTTVMIGHHQ LAREVLLKKG KEFSGRPKVA 100
TLDILSDNQK GIAFADHGAH WQLHRKLVLN AFALFKDGNL KLEKIINQEA 150
NVLCDFLATQ HGQSIDLSEP LSLAVTNIIS FICFNFSFKN EDPALKAIQN 200
VNDGILEVLG KEVLLDIFPA LKIFPSKAME KMKGCVETRN ELLSEILEKC 250
QENFTSDSIT NLLHILMQAK VNADNNNTGP EQDSKLLSNR HMLATIADIF 300
GAGVETTTSV IKWIVAYLLH HPSLKKRIQD SIDQNIGFNR TPTISDRNRL 350
VLLEATIREV LRIRPVAPML IPHKAIIDSS IGDLTIDKGT DVVVNLWALH 400
HNEKEWQQPD LFMPERFLDP TGTQLISPSL SYLPFGAGPR SCVGEMLARQ 450
ELFLFMSRLL QRFNLEIPDD GKLPSLEGNP SLVLQIKPFK VKIEVRQAWK 500
EAQAEGSTS 509
Length:509
Mass (Da):57,308
Last modified:January 4, 2005 - v2
Checksum:iD498E639D36A5148
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti210 – 2101G → S in AAA63517. 1 Publication
Sequence conflicti464 – 4641N → Y in AAA63517. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L40335 mRNA. Translation: AAA63517.1.
AF251388 mRNA. Translation: AAF65824.1.
RefSeqiNP_001009483.1. NM_001009483.1.
UniGeneiOar.399.

Genome annotation databases

GeneIDi493968.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L40335 mRNA. Translation: AAA63517.1 .
AF251388 mRNA. Translation: AAF65824.1 .
RefSeqi NP_001009483.1. NM_001009483.1.
UniGenei Oar.399.

3D structure databases

ProteinModelPortali Q29497.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 493968.

Organism-specific databases

CTDi 1586.

Phylogenomic databases

HOVERGENi HBG106944.

Enzyme and pathway databases

UniPathwayi UPA00062 .

Family and domain databases

Gene3Di 1.10.630.10. 1 hit.
InterProi IPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view ]
Pfami PF00067. p450. 1 hit.
[Graphical view ]
PRINTSi PR00463. EP450I.
PR00385. P450.
SUPFAMi SSF48264. SSF48264. 1 hit.
PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning and expression of ovine cytochrome P-450 17-alpha hydroxylase/c17-20 lyase."
    Murry B.A., Swart P., Mason J.I.
    Submitted (MAR-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Adrenal cortex.
  2. "Molecular cloning and heterologous expression in E. coli of cytochrome P45017alpha. Comparison of structural and functional properties of substrate-specific cytochromes P450 from different species."
    Gilep A.A., Estabrook R.W., Usanov S.A.
    Biochemistry (Mosc.) 68:86-98(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Adrenal gland.

Entry informationi

Entry nameiCP17A_SHEEP
AccessioniPrimary (citable) accession number: Q29497
Secondary accession number(s): Q9N0U6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: January 4, 2005
Last modified: July 9, 2014
This is version 90 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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