Reviewed, UniProtKB/Swiss-Prot Q29497 (CP17A_SHEEP)

Names and origin

Protein names

Recommended name:
    Steroid 17-alpha-hydroxylase/17,20 lyase
    EC=1.14.99.9
Alternative name(s):
    Cytochrome P450 17A1
    CYPXVII
    P450-C17
      Short name=P450c17

Gene names

Name:	CYP17A1
Synonyms:	CYP17

Organism

Ovis aries (Sheep)

Taxonomic identifier

9940 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Caprinae › Ovis

Protein attributes

Sequence length	509 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Conversion of pregnenolone and progesterone to their 17-alpha-hydroxylated products and subsequently to dehydroepiandrosterone (DHEA) and androstenedione. Catalyzes both the 17-alpha-hydroxylation and the 17,20-lyase reaction. Involved in sexual development during fetal life and at puberty.
Catalytic activity	A steroid + AH2 + O2 = a 17-alpha-hydroxysteroid + A + H2O.
Cofactor	Heme group By similarity.
Enzyme regulation	Regulated predominantly by intracellular cAMP levels By similarity.
Pathway	Lipid metabolism; steroid biosynthesis.
Subcellular location	Membrane Potential. Membrane; Single-pass membrane protein.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Biological process	Steroidogenesis
Cellular component	Membrane
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW steroid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro steroid 17-alpha-monooxygenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 509	509	Steroid 17-alpha-hydroxylase/17,20 lyase		PRO_0000051942
Sites
	Metal binding	442	1	Iron (heme axial ligand) By similarity
Experimental info
	Sequence conflict	210	1	G → S in AAA63517. Ref.1
	Sequence conflict	464	1	N → Y in AAA63517. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q29497-1 [UniParc]. Last modified January 4, 2005. Version 2. Checksum: D498E639D36A5148	FASTA	509	57,308
	10 20 30 40 50 60 MWVLLAVFLL TLAYLFWPKT KHSGAKYPRS LPSLPLVGSL PFLPRRGQQH ENFFKLQEKY 70 80 90 100 110 120 GPIYSFRLGS KTTVMIGHHQ LAREVLLKKG KEFSGRPKVA TLDILSDNQK GIAFADHGAH 130 140 150 160 170 180 WQLHRKLVLN AFALFKDGNL KLEKIINQEA NVLCDFLATQ HGQSIDLSEP LSLAVTNIIS 190 200 210 220 230 240 FICFNFSFKN EDPALKAIQN VNDGILEVLG KEVLLDIFPA LKIFPSKAME KMKGCVETRN 250 260 270 280 290 300 ELLSEILEKC QENFTSDSIT NLLHILMQAK VNADNNNTGP EQDSKLLSNR HMLATIADIF 310 320 330 340 350 360 GAGVETTTSV IKWIVAYLLH HPSLKKRIQD SIDQNIGFNR TPTISDRNRL VLLEATIREV 370 380 390 400 410 420 LRIRPVAPML IPHKAIIDSS IGDLTIDKGT DVVVNLWALH HNEKEWQQPD LFMPERFLDP 430 440 450 460 470 480 TGTQLISPSL SYLPFGAGPR SCVGEMLARQ ELFLFMSRLL QRFNLEIPDD GKLPSLEGNP 490 500 SLVLQIKPFK VKIEVRQAWK EAQAEGSTS

References

[1]	"Cloning and expression of ovine cytochrome P-450 17-alpha hydroxylase/c17-20 lyase." Murry B.A., Swart P., Mason J.I. Submitted (MAR-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Adrenal cortex.
[2]	"Molecular cloning and heterologous expression in E. coli of cytochrome P45017alpha. Comparison of structural and functional properties of substrate-specific cytochromes P450 from different species." Gilep A.A., Estabrook R.W., Usanov S.A. Biokhimiia 68:86-98(2003) [PubMed: 12693981] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Adrenal gland.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L40335 mRNA. Translation: AAA63517.1. AF251388 mRNA. Translation: AAF65824.1.
RefSeq	NP_001009483.1.
UniGene	Oar.399
3D structure databases
HSSP	HSSP built from PDB template 1IO7 based on UniProtKB Q55080.
ModBase	Search...
Genome annotation databases
GeneID	493968.
Organism-specific databases
CTD	493968.
Phylogenomic databases
HOVERGEN	Q29497.
Enzyme and pathway databases
BRENDA	1.14.99.9. 271.
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR017973. Cyt_P450_C. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHER	PTHR19383. Cyt_P450. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00385. P450.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CP17A_SHEEP

Accession

Primary (citable) accession number: Q29497
Secondary accession number(s): Q9N0U6

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	January 4, 2005
Last modified:	October 13, 2009
This is version 65 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)