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Protein

Glucose-6-phosphate 1-dehydrogenase

Gene

G6PD

Organism
Macropus robustus (Wallaroo) (Euro)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis (By similarity).By similarity

Catalytic activityi

D-glucose 6-phosphate + NADP+ = 6-phospho-D-glucono-1,5-lactone + NADPH.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei72 – 721NADP 1By similarity
Binding sitei147 – 1471NADP 1By similarity
Binding sitei171 – 1711NADP 1; via carbonyl oxygenBy similarity
Binding sitei171 – 1711SubstrateBy similarity
Binding sitei239 – 2391SubstrateBy similarity
Binding sitei258 – 2581SubstrateBy similarity
Active sitei263 – 2631Proton acceptorBy similarity
Binding sitei357 – 3571NADP 2By similarity
Binding sitei360 – 3601SubstrateBy similarity
Binding sitei365 – 3651SubstrateBy similarity
Binding sitei366 – 3661NADP 2By similarity
Binding sitei370 – 3701NADP 2By similarity
Binding sitei393 – 3931NADP 2By similarity
Binding sitei395 – 3951SubstrateBy similarity
Binding sitei487 – 4871NADP 2By similarity
Binding sitei503 – 5031NADP 2By similarity
Binding sitei509 – 5091NADP 2By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi38 – 458NADP 1By similarity
Nucleotide bindingi401 – 4033NADP 2By similarity
Nucleotide bindingi421 – 4233NADP 2By similarity

GO - Molecular functioni

  1. glucose-6-phosphate dehydrogenase activity Source: UniProtKB
  2. NADP binding Source: InterPro

GO - Biological processi

  1. glucose 6-phosphate metabolic process Source: UniProtKB
  2. glucose metabolic process Source: UniProtKB-KW
  3. NADP metabolic process Source: UniProtKB
  4. pentose-phosphate shunt Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Carbohydrate metabolism, Glucose metabolism

Keywords - Ligandi

NADP

Enzyme and pathway databases

UniPathwayiUPA00115; UER00408.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucose-6-phosphate 1-dehydrogenase (EC:1.1.1.49)
Short name:
G6PD
Gene namesi
Name:G6PD
OrganismiMacropus robustus (Wallaroo) (Euro)
Taxonomic identifieri9319 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaMetatheriaDiprotodontiaMacropodidaeMacropus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 515514Glucose-6-phosphate 1-dehydrogenasePRO_0000068084Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei89 – 891N6-acetyllysineBy similarity
Modified residuei171 – 1711N6-acetyllysineBy similarity
Modified residuei403 – 4031N6-acetyllysineBy similarity
Modified residuei432 – 4321N6-acetyllysineBy similarity
Modified residuei497 – 4971N6-acetyllysineBy similarity

Post-translational modificationi

Acetylated by ELP3 at Lys-403; acetylation inhibits its homodimerization and enzyme activity. Deacetylated by SIRT2 at Lys-403; deacetylation stimulates its enzyme activity (By similarity).By similarity

Keywords - PTMi

Acetylation

Proteomic databases

PRIDEiQ29492.

Interactioni

Subunit structurei

Homotetramer; dimer of dimers. Interacts with SIRT2; the interaction is enhanced by H2O2 treatment (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliQ29492.
SMRiQ29492. Positions 28-515.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni201 – 2055Substrate bindingBy similarity

Sequence similaritiesi

Phylogenomic databases

HOVERGENiHBG000856.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00966. G6PD.
InterProiIPR001282. G6P_DH.
IPR019796. G6P_DH_AS.
IPR022675. G6P_DH_C.
IPR022674. G6P_DH_NAD-bd.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR23429. PTHR23429. 1 hit.
PfamiPF02781. G6PD_C. 1 hit.
PF00479. G6PD_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000110. G6PD. 1 hit.
PRINTSiPR00079. G6PDHDRGNASE.
TIGRFAMsiTIGR00871. zwf. 1 hit.
PROSITEiPS00069. G6P_DEHYDROGENASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q29492-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEQVALSRT QVCGILREEL YQGDAFHQSD THIFIIMGAS GDLAKKKIYP
60 70 80 90 100
TIWWLFRDGL LPEDTFIVGY ARSNLTVDDI RKQSEPYFKA TPEENLKLEE
110 120 130 140 150
FFSRNSYVAG QYDEPASFQR LNAHMNSLHH GSQANRLFYL ALPPTVYEAV
160 170 180 190 200
TKNIKETCMS QIGWNRVIVE KPFGKDLQSS DKLSNHISSL FHEDQIYRID
210 220 230 240 250
HYLGKEMVQN LMVLRFGNRI FGPIWNRDNI ACVIFTFKEP FGTLGRGGYF
260 270 280 290 300
DEFGIIRDVM QNHLLQMLCL VAMEKPASTN SDDVRDEKVK VLKCISEVRA
310 320 330 340 350
TDVVLGQYVG NPDGEGEATK GYLDDPTVPR GSTTATFAAV VLYVENERWD
360 370 380 390 400
GVPFILRCGK ALNERKAEVR LQFRDVAGDI FQRQCKRNEL VIRVQPNEAV
410 420 430 440 450
YTKMMTKKPG MFFNPEESEL DLTYGNRYKD VKLPDAYERL ILDVFCGSQM
460 470 480 490 500
HFVRSDELRE AWRIFTPLLH HIEKEKTQPI AYVYGSRGPP EADELMKRVG
510
FQYEGTYKWV NPHKL
Length:515
Mass (Da):59,315
Last modified:January 22, 2007 - v3
Checksum:iD49BA95185A78952
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U13899 mRNA. Translation: AAA76599.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U13899 mRNA. Translation: AAA76599.1.

3D structure databases

ProteinModelPortaliQ29492.
SMRiQ29492. Positions 28-515.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiQ29492.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

HOVERGENiHBG000856.

Enzyme and pathway databases

UniPathwayiUPA00115; UER00408.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00966. G6PD.
InterProiIPR001282. G6P_DH.
IPR019796. G6P_DH_AS.
IPR022675. G6P_DH_C.
IPR022674. G6P_DH_NAD-bd.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR23429. PTHR23429. 1 hit.
PfamiPF02781. G6PD_C. 1 hit.
PF00479. G6PD_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000110. G6PD. 1 hit.
PRINTSiPR00079. G6PDHDRGNASE.
TIGRFAMsiTIGR00871. zwf. 1 hit.
PROSITEiPS00069. G6P_DEHYDROGENASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Full-length cDNA sequence of X-linked G6PD of an Australian marsupial, the wallaroo."
    Loebel D.A., Longhurst T.J., Johnston P.G.
    Mamm. Genome 6:198-201(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Liver.

Entry informationi

Entry nameiG6PD_MACRO
AccessioniPrimary (citable) accession number: Q29492
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 1997
Last sequence update: January 22, 2007
Last modified: March 3, 2015
This is version 85 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.